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Citations for Thermo Fisher :
8151 - 8200 of 10000+ citations for Killer cell immunoglobulin like receptor 2DL3 KIR2DL3 Human HEK293 His since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2024Quote: ... Cells are passaged as single cells using TrypLE Express (Thermo Fisher Scientific) and split at a ratio of 1:10 to 1:100 as needed at 70-90% confluence ...
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bioRxiv - Molecular Biology 2024Quote: For cell cultures cells were detached with 0.25% Trypsin-EDTA (Gibco, 25200056) for 5 min at 37°C ...
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bioRxiv - Immunology 2024Quote: ... HAP1 WT cells were labelled with cell trace violet (Thermo Fisher Scientific), HAP1 SPPL3-/- cells with AF350 succinimidyl ester (Invitrogen ...
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bioRxiv - Immunology 2024Quote: ... Molt-4 cells and yac-1 cells were cultured RPMI1640 (Gibco, USA) supplemented with 10% FBS ...
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bioRxiv - Immunology 2024Quote: E0771 cells and MC38 cells were collected using 0.25% Trypsin-EDTA (Gibco). The cells were washed twice with PBS ...
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bioRxiv - Cancer Biology 2024Quote: ... Cells were then treated with 1mL red blood cell lysis buffer (Thermofisher) on ice for 90 seconds ...
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bioRxiv - Cancer Biology 2024Quote: ... Cells were then treated with 1mL red blood cell lysis buffer (Thermofisher) on ice for 90 seconds ...
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bioRxiv - Neuroscience 2024Quote: ... Single cells were dissociated using StemPro Accutase Cell Dissociation Reagent (Life Technologies), filtered through 30 uM and 20 uM filters (Miltenyi Biotec ...
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bioRxiv - Immunology 2024Quote: ... Cells were stained in LIVE/DEAD Fixable Yellow Dead Cell Stain (ThermoFisher) for 30 minutes prior to surface staining ...
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bioRxiv - Biochemistry 2024Quote: ... aliquots of cells were frozen down in Recovery Cell Culture Medium (Gibco) and passaging was continued in regular media supplemented with 200 µg/mL G418 in the absence of pen/strep ...
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bioRxiv - Biophysics 2024Quote: ... All cell quantifications were performed using the Countess Automated Cell Counter (Invitrogen).
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bioRxiv - Biochemistry 2024Quote: ... Cell viability was assessed using the alamarBlue HS Cell Viability Reagent (Invitrogen) and a FLUOstar Optima microplate reader ...
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bioRxiv - Genetics 2024Quote: ... Cells were imaged with EVOS FL Cell Imaging System (Thermo Fisher Scientific).
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bioRxiv - Synthetic Biology 2024Quote: ... Cells were counted using a Countess 3 Automated Cell Counter (Thermo Fisher) by performing four counts on the same chip and using the average cell number to increase seeding precision ...
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bioRxiv - Biochemistry 2024Quote: ... Cells were cultured in ESF 921 insect cell culture medium (Thermo Scientific).
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bioRxiv - Bioengineering 2024Quote: ... Cells were then analysed using Countess™ 3 Automated Cell Counter (Invitrogen) to determine cell survival.
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bioRxiv - Biochemistry 2024Quote: ... cells were dissociated from plate using cell dissociation Buffer (ThermoFisher Scientific, #13150016) and were stained with LIVE/DEAD™ Fixable Violet Dead Cell Stain Kit ...
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bioRxiv - Cell Biology 2024Quote: Cell proliferation was assessed using the PrestoBlue™ Cell Viability Reagent (Invitrogen). Briefly ...
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bioRxiv - Cancer Biology 2024Quote: ... Cell number was counted with Countess II Cell Counter (Thermo Fisher Scientific). A total of 50,000 cells (12,500 cells per sample ...
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bioRxiv - Cancer Biology 2024Quote: ... Tumor digests or cell lines were labeled with cell-trace violet (Thermofisher, 1:4000 in 5 ml PBS ...
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bioRxiv - Cell Biology 2024Quote: ... Cells were dissociated into single cell suspension using TrypLE Express (ThermoFisher Scientific). The last one was incubated with 1% FBS solution to block nonspecific interactions for 30 min at RT with a DyLight 488-conjugated TRA-1-60 antibody (ThermoFisher Scientific ...
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bioRxiv - Cancer Biology 2024Quote: ... One million cells were counted using the cell counter Countess II (Invitrogen) and pelleted for protein extraction with Cell Lysis Buffer (Cell Signaling Technology Catalog No ...
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bioRxiv - Evolutionary Biology 2024Quote: ... We used an automated cell counter (Cell Countess II FL, Thermo Fisher) to quantify the total number of spores ...
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bioRxiv - Evolutionary Biology 2024Quote: ... Cell viability was assessed on a Countess II automated cell counter (ThermoFisher), and an estimated 12,000 cells were loaded onto one lane of a 10x Genomics Chromium Controller ...
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bioRxiv - Cell Biology 2024Quote: ... Dead cells were excluded using Tail Cell Cycle Solution (Invitrogen, Cat# A10798).Following doublet exclusion and single-cell population isolation through forward and side scatter analysis ...
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bioRxiv - Cell Biology 2020Quote: ... Each labeled sample was assayed with the Affymetrix PrimeView human gene expression array read with an Affymetrix GeneChip Scanner 3000 (Affymetrix/Thermo Fisher Scientific, Waltham, MA, USA).
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Dual signaling via interferon and DNA damage response elicits entrapment by giant PML nuclear bodiesbioRxiv - Microbiology 2021Quote: ... cells were isolated from human foreskin tissue and cultivated at 37 °C and 5% CO2 in Eagle’s minimal essential medium (Gibco, Thermo Fisher Scientific Inc., Waltham, MA, USA) containing 7 % fetal calf serum (FCS ...
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bioRxiv - Neuroscience 2020Quote: The gene expression data for the human cerebral cortex used in this study is downloaded from the NCBI Gene Expression Omnibus under the accession number GSE25219 (Affymetrix Human Exon 1.0 ST Array)(43) ...
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bioRxiv - Cancer Biology 2020Quote: Human CD34+ cells were isolated from patient bone marrow samples by erythrocyte lysis buffer treatment, followed by separation with AutoMACs sorter and CD34 Microbead Kit human (MiltenyiBiotech, Germany) and grown in IMDM (Gibco, Thermo Fisher Scientific, CA, USA) supplemented with 2% FBS (Gibco ...
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bioRxiv - Cell Biology 2021Quote: ... Human islets were purified using Ficoll density gradients [31] and cultured in Connaught Medical Research Laboratories (CMRL) 1066 medium (Invitrogen, Life Technologies, Carlsbad, CA, USA) supplemented with 10% fetal calf serum ...
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bioRxiv - Cancer Biology 2022Quote: ... Cas9 over expressing cells were transduced with lentiviruses expressing gRNA with no sequence homology to any region of the human genome (LentiArray™ CRISPR Negative Control Lentivirus, Thermo Fisher Scientific, Cat # A32327).
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... Epi-Life® Growth Medium with 60 µM calcium and Cascade Biologist human keratinocyte growth supplement (HKGS) 100X (S-001–5) were from Life Technologies (Grand Island, NY, USA). The Dulbecco’s phosphate-buffered saline (DPBS) ...
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bioRxiv - Immunology 2022Quote: ... operated by UNICORN 6.4, using HiTrap Protein A columns (Cytiva, catalog no. GE17-5079-01) for human IgG1 and CaptureSelect™ CH1-XL (Thermo Fisher, catalog no. 494346201) for Fab fragments ...
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bioRxiv - Molecular Biology 2020Quote: ... Expression profiling of the kidney biopsies was previously carried out using Affymetrix GeneChips (HumanGenome U133 Array and U133Plus2 Array and Affymetrix Human Gene ST GeneChip 2.1) as reported (35,36) ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... parasites were maintained in vitro essentially as described previously,12 in human O- erythrocytes suspended in HEPES- and GlutamaxTM-supplemented RPMI 1640 (Thermo Fisher Scientific, order number 72400120) to which 11 mM D-glucose ...
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bioRxiv - Microbiology 2020Quote: ... S100 Calcium-binding protein A7 (S100A7) and human β-defensin 2 (DEFB4A) were amplified by using TAQMAN Gene Expression Assays (Applied Biosystems, Foster City, CA). TaqMan probes were grafted with a fluorophore (FAM ...
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bioRxiv - Developmental Biology 2020Quote: ... the ells were transfected with human EC-SOD (hEC-SOD) cDNA inserted into a vector plasmid pcDNA3 (5446 nucleotides; Invitrogen Life Technologies, Carlsbad, CA, USA) as previously described,14 using the FuGENE kit (Roche Diagnostics ...
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bioRxiv - Immunology 2020Quote: ... Immune mediator levels in COVID-19 patient plasma samples across acute samples were measured with by Luminex using the Cytokine/Chemokine/Growth Factor 45-plex Human ProcartaPlex™ Panel 1 (ThermoFisher Scientific, #EPX450-12171-901). Data acquisition was performed on FLEXMAP® 3D (Luminex ...
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bioRxiv - Immunology 2021Quote: ... with an N-terminal IL-2 signal peptide for secretion and a Fc part (hinge-CH2-CH3) of human IgG1 for purification was inserted into pFUSE-mIgG1-Fc2 vector (Invitrogen, Thermo Fisher Scientific, MA, USA). The construct (RBD-huFc ...
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bioRxiv - Immunology 2022Quote: ... from normal donors were obtained with an NIH-approved protocol and activated with CD3 and CD28 microbeads at a ratio of 1:3 (Dynabeads Human T-Expander CD3/CD28, Thermo Fisher Scientific, catalog no. 11141D) in AIM-V media containing 40 IU/mL recombinant IL-2 and 5% FBS for 24 hr ...
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bioRxiv - Immunology 2023Quote: ... were seeded in Ultraculture complete medium and partially activated with dynabeads human T-activator CD3/CD28 (final dilution 1:1000) (Thermo Fisher Scientific, Waltham, MA USA) or completely activated with dynabeads human T-activator CD3/CD28 and IL2 2.5 U/ml ...
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bioRxiv - Neuroscience 2023Quote: ... which specifically targets on the phosphorylated Thr188 of murine C/EBPβ (a conservative locus to Thr235 of human C/EBPβ) in eBioscience™ Transcription Factor Staining Buffer Set (Invitrogen, Catalog #00-5523-00). For background signal staining ...
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bioRxiv - Pathology 2024Quote: ... primary dermal fibroblasts (normal, human, adult; PCS-201-012, ATCC, VA, USA) were cultured in Dulbecco’s Modified Eagle medium (DMEM) (Thermo Fisher Scientific Inc., Waltham, MA, USA) supplemented with 10% fetal bovine serum (FBS ...
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bioRxiv - Immunology 2024Quote: The amounts of TNF-α and IL-6 in culture supernatants of LPS or Pam3cys-re-stimulated IS-trained macrophages were quantified using commercial human ELISA kits (Thermo Fisher Scientific, Waltham, MA, USA). Optical density was measured using the Infinite M200 (Tecan ...
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bioRxiv - Neuroscience 2023Quote: ... and growth factors was performed using a customized multiplex bead-based immunoassay - Immune Monitoring 65-Plex Human ProcartaPlex™ Panel (#EPX650-10065-901; ThermoFisher Scientific, Waltham, MA, US) according to manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2023Quote: ... supplemented with 5% fetal bovine serum except for RWPE-1 and RWPE-2 cell lines which were grown in a keratinocyte serum-free medium supplemented with 5 ng/mL recombinant human epidermal growth factor (EGF) and 50 μg/mL bovine pituitary extract (Gibco™, Invitrogen, Carlsbad, CA, USA). The cell lines were tested for short tandem repeat (STR ...
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bioRxiv - Cell Biology 2023Quote: NHK/38 cells and sections of normal human epidermis were stained with a monoclonal anti-hIL-38 antibody (H127C) (14-7385-82, Thermo Fisher Scientific AG, 1/2000), followed by an Alexa Fluor 594-labeled goat anti-mouse IgG2b (115-585-207 ...
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bioRxiv - Genomics 2023Quote: ... 5 U/mL, human Chorionic Gonadotropin-hCG (Chorulon®, Intervet International B. V., Boxmeer, Netherland) and 50 μg/mL gentamicin (Gibco Thermo Fisher, Massachusets - USA). Culture droplets were covered with mineral oil and placed in an incubator at 38.5°C and a humid atmosphere of 5% CO2 in atmospheric air ...
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bioRxiv - Neuroscience 2024Quote: ... The expression of specific proteins in NDEs were quantified by ELISA kits for human P-serine 312-IRS-1 (Life Technologies Corporation, Carlsbad, CA, USA), human P-pan-tyrosine-IRS-1 (Cell Signaling Technology ...
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bioRxiv - Biophysics 2021Quote: ... Coli TOP10 cells (Invitrogen) were used for plasmid propagation and isolation ...