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Citations for Thermo Fisher :
8251 - 8300 of 10000+ citations for Killer cell immunoglobulin like receptor 2DL3 KIR2DL3 Human HEK293 His since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2022Quote: ... cells were FACS sorted directly into 5 or 10μl Single cell lysis solution (SCLS; Single cell lysis kit Invitrogen, cat# 4458235) with DNase I according to manufacturer’s instructions with the exception that the lysis reaction was incubated for 15min at room temperature (RT ...
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bioRxiv - Neuroscience 2024Quote: Intracellular ROS in BV-2 cells was measured by incubating cells with 1 µM cell-permeant 2’,7’-dichlorodihydrofluorescein diacetate (H2DCFDA, Invitrogen, D399) at 37°C for 30 min.
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bioRxiv - Immunology 2024Quote: ... These cells were cultured with purified OVA-specific CD4+ or CD8+ T cells that were stained with a cell proliferation dye (Cell Trace Violet, ThermoFisher Scientific) for three days (100,000 cells/well) ...
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bioRxiv - Microbiology 2023Quote: ... Percentage of mast cells among the peritoneal cell population was determined after staining an aliquot of cells with Hema3 kit (Fisher Scientific).
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bioRxiv - Microbiology 2023Quote: ... bacmids were isolated by isopropanol precipitation and used to transfect Sf9 cells at a cell density of 1 million cells/ml in Sf-900™ II serum-free media (Gibco). First generation baculoviruses (P1 ...
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bioRxiv - Developmental Biology 2023Quote: ... 50 cells were sorted into 10 μL of Single cell lysis buffer supplemented with DNAseI (Single Cell-to-CT Kit ThermoFisher, 4458237) and stored at -80°C ...
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bioRxiv - Immunology 2023Quote: ... cell pellets were re-suspended and red blood cells were removed using Red Blood Cell Lysis Buffer (Thermo Fisher Scientific, A1049201). The total cell count was determined using a cell counting chamber under light microscopy ...
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bioRxiv - Pathology 2023Quote: ... cells were counted and critically assessed for single cell separation and overall cell viability using the Countess 3 FL (Fisher Scientific). Samples were then stained for multiplexing using cell hashing [29] ...
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bioRxiv - Immunology 2023Quote: Spleen cells were prepared for intracellular staining as described under “Flow cytometry and cell sorting” with the following modifications: cells were stained with the Live/Dead Fixable Aqua Dead Cell Stain Kit (Invitrogen, L34966) for 30 min at 4°C (1:500 dilution in PBS ...
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bioRxiv - Cell Biology 2023Quote: ... First, cell detachment was attained using a cell dissociation solution (at 2:2:1 ratio, Cell Dissociation Buffer (Thermo Fisher Scientific): RPMI 1640 ...
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bioRxiv - Developmental Biology 2023Quote: ... The cell pellet was washed twice and lyse the cells with RIPA cell lysis and extraction buffer (Thermo Scientific™, 89900) and 1x Protease Inhibitor cocktail (PIC ...
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bioRxiv - Cancer Biology 2023Quote: ... we changed medium to LG/LF medium without added estrogen and cultured cells overnight before harvesting cells with cell dissociation buffer (Gibco, ThermoFisher Scientific). We resuspended cells at 1 x 105 cells per 100 µl sterile 0.9% NaCl for intracardiac injection ...
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bioRxiv - Biochemistry 2023Quote: ... and used to infect Sf21 insect cells at a cell density of 1.0 x 106 cells/mL in Sf-900 SFM medium (Gibco Life Technologies), shaking at 90 rpm on Sartorius Stedim Biotech “CERTOMAT RM” with a 25mm orbit ...
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bioRxiv - Immunology 2023Quote: ... DCs and CD4+ T cells were washed and resuspended in RPMI supplemented with 2% FCS at a cell density of 1×107 cells/ml and labelled with 7.5μM Cell Tracker Red CMTPX (Thermofisher; Waltham, MA, USA) or 7.5μM vibrant CFDA SE (CFSE ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... was used to detach the cells during passage and the number of live cells was calculated with an automatic cell counter (Countess II, Thermo Fisher) after staining the cells with trypan blue (Sigma ...
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bioRxiv - Microbiology 2024Quote: ... cells were seeded at 50,000 cells per well in 12 well plates by detaching cells using StemPro Accutase Cell Dissociation Reagent (Thermo Fisher Scientific), and seeding in plates coated with Matrigel (Sigma) ...
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bioRxiv - Cancer Biology 2021Quote: ... Cell viability was measured using alamarBlue Cell Viability Reagent (DAL1100, Thermo Fisher Scientific) according to the manufacturer’s instructions.
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bioRxiv - Molecular Biology 2020Quote: Mouse ES cells were dissociated into single cells by incubation with TrypLE (Gibco). The ells were washed and diluted in PBS ...
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bioRxiv - Cancer Biology 2021Quote: ... Seahorse XF Cell culture microplates were treated with Cell Tak (Thermo Fisher Scientific) according to the manufacturer’s instructions and number of viable cells was determined using Countess Automated cell coulter with trypan blue stain before plated in 22 or more replicates at a density of 25000 cells/ well ...
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bioRxiv - Cell Biology 2020Quote: ... Cells were transferred into cell culture media or Hanks’ balanced salt solution (Invitrogen) with 10 mM HEPES (Sigma-Aldrich ...
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bioRxiv - Cell Biology 2020Quote: ... 1×106 cells were plated on cell culture inserts (140640, Thermo Fisher scientific), and 4 h later unadherent cells were removed by medium change ...
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bioRxiv - Cell Biology 2020Quote: Cell number was counted with a Countess II FL Automated Cell Counter (ThermoFisher) at indicated time intervals ...
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bioRxiv - Cell Biology 2020Quote: ... Whenever cells were dissociated into single cells using 0.5 × TrypLE Select (Thermo Fisher) or Accumax (Sigma Aldrich) ...
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bioRxiv - Immunology 2021Quote: ... T cells were labelled with the CellTrace CFSE Cell Proliferation Kit (C34554 ThermoFisher) following manufacturer’s instructions ...
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bioRxiv - Cell Biology 2020Quote: ... The cells were then counted using a Countess II cell counter (Thermo Fisher), and the number of Trypan-positive cells recorded ...
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bioRxiv - Immunology 2021Quote: ... T-cells were labeled with Cell Trace Violet (CTV) dye (Thermo Fisher Scientific) for 20 min at 37°C at a final concentration of 2.5 μM in PBS ...
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bioRxiv - Immunology 2021Quote: ... T-cells were labeled with Cell Trace Violet (CTV) dye (Thermo Fisher Scientific) for 20 min at 37°C at a final concentration of 2.5 μM in PBS ...
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bioRxiv - Immunology 2021Quote: ... cells were incubated before cell surface staining with Hoechst 33342 Fluorescent Stain (Invitrogen) at a final concentration of 1 μg/ml in Dulbecco’s phosphate-buffered saline (DPBS ...
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bioRxiv - Immunology 2021Quote: ... expression on live cells (LIVE/DEAD Fixabable Near-IR Dead Cell Stain; Invitrogen) was confirmed by flow cytometry after 7 days of 0.8 μg/ml puromycin dihydrochloride selection.
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bioRxiv - Immunology 2021Quote: ... cells were labelled with Cell Proliferation Dye eF450 (10 μM, Thermo Fisher Scientific) in PBS for 10 min at 37°C before quenching with FCS-containing media ...
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bioRxiv - Developmental Biology 2021Quote: ... the cell pellet was resuspended in Recovery™ Cell Culture Freezing Medium (Gibco), the solution transferred to cryo vials and then cooled down in CoolCell™ LX Freezing Containers (Merck ...
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bioRxiv - Genetics 2019Quote: ... cells were diluted to 1 cell/100βl in Dulbecco modified Eagle medium (Gibco) containing 20% FBS ...
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bioRxiv - Molecular Biology 2021Quote: Cells were labelled with LIVE/DEAD™ Aqua Dead Cell Stain Kit (Invitrogen) and then fixed in Intracellular Staining Fixation Buffer (BioLegend ...
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bioRxiv - Microbiology 2021Quote: ... cell proliferation was assessed using the Vybrant MTT Cell Proliferation Assay Kit (Invitrogen). Media was completely removed from cells and replaced with 100 μl of fresh growth media ...
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Reconstitution of prospermatogonial specification in vitro from human induced pluripotent stem cellsbioRxiv - Developmental Biology 2020Quote: ... cells were strained through a 70 µm nylon cell strainer (Thermo Fisher Scientific). The AG+ cells were sorted by FACSAria Fusion (BD Biosciences ...
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bioRxiv - Neuroscience 2021Quote: ... cell concentrations were quantified using the Countess II Automated Cell Counter (Life Technologies) and 10K-16K cells were loaded per single-cell RNA capture reaction ...
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bioRxiv - Molecular Biology 2021Quote: ... Cell suspension is filtered through 100 μm nylon cell strainer (Fisher Scientific #11893402), treated by DNase I (Sigma D4527 ...
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bioRxiv - Cell Biology 2022Quote: ... For live cell imaging experiments cells were imaged in Leibovitz L-15 (Thermofisher) medium supplemented with 10% FBS ...
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bioRxiv - Biochemistry 2019Quote: ... Transient Transfection of HTT in Mammalian Cells: EXPI293F cells (Thermo Fisher, Cat. A14527) were maintained in EXPI293 Expression Medium (Thermo Fisher ...
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bioRxiv - Cell Biology 2020Quote: ... coverslips with cells were mounted in Attofluor Cell chambers (Thermo Fisher Scientific; A7816) in complete CO2-independent medium (Thermo Fisher Scientific ...
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Redundant and specific roles of cohesin STAG subunits in chromatin looping and transcription controlbioRxiv - Cell Biology 2019Quote: ... fixed and stained cells were mounted in an attofluorTM cell chamber (Thermo Fisher) and and 1ml of dSTORM buffer (25mM MEA (Sigma) ...
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bioRxiv - Microbiology 2019Quote: ... 293T cells (ATCC) and NIH 3T3 cells (ATCC) were maintained in DMEM (Invitrogen) supplemented with 10% fetal bovine serum (FBS) ...
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bioRxiv - Immunology 2020Quote: ... The cells were separated into single cell suspension using accutase (Thermo Fisher Scientific) for GSCs and trypsin for the attached astrocytes ...
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bioRxiv - Developmental Biology 2019Quote: ... adherent cells were detached from the well by using Cell Dissociation Buffer (ThermoFisher). Cells were washed with PBS + 1% BSA ...
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bioRxiv - Cancer Biology 2019Quote: ... and cell concentration determined using a Countess II Automated Cell Counter (Thermo Scientific). Single cell library preparation was performed using a Chromium Single Cell 3’ Reagent Kit v3 (10X Genomics ...
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bioRxiv - Cell Biology 2019Quote: ... cells were incubated for 15 min with Cell Mask (1:1000; Thermofisher Switzerland) in PBS before mounting the coverslips with VECTASHIELD with DAPI (Vector Laboratories) ...
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bioRxiv - Bioengineering 2020Quote: ... target cells were pre-labeled with Cell Trace CSFE dye (C34570, Molecular Probes) according to the manufacturer’s instructions.
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bioRxiv - Bioengineering 2019Quote: ... This cell line was derived from CHO-S cells (Gibco, Catalog No. A11364). In order to find the best combination of basal media and feed that could result in optimum cell growth ...
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bioRxiv - Microbiology 2019Quote: ... PGC fibroblast cells were maintained in cell culture media (Knockout DMEM (10829018, Gibco) with 10% ES grade FBS (16141061 ...
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bioRxiv - Microbiology 2019Quote: ... cells were incubated with 30 nM of SYTOX Green Dead Cell Stain (Invitrogen) for 15 minutes ...