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Citations for Thermo Fisher :
7951 - 8000 of 10000+ citations for Killer cell immunoglobulin like receptor 2DL3 KIR2DL3 Human HEK293 His since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2020Quote: ... the cells were stained for living cells using LIVE/DEAD® Fixable Near-IR Dead Cell Stain (L10119, ThermoFisher Scientific) (dilution 1:500 ...
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bioRxiv - Cancer Biology 2020Quote: ... Cancer cells were isolated from the tumor cell pellets and dissociated into single tumor cells with TrypLE Express Enzyme (#12605010, Gibco) containing DNase I at 37 °C for 10 minutes.
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bioRxiv - Genomics 2021Quote: ... Washed cells were immediately counted in a Trypan blue stain-based Cell Countess II Automated Cell Counter (Thermo Scientific #AMQAX1000).
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bioRxiv - Microbiology 2022Quote: ... The percentage of transfected or transduced cells (GFP+ cells) was determined by gating the living cell population based on viability dye staining (Aqua Vivid, Invitrogen). Samples were acquired on an LSRII cytometer (BD Biosciences ...
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bioRxiv - Immunology 2022Quote: ... and 125) with TSt-4 (B cells) or TSt-4/DLL1 stromal cells (T cells) in RPMI (Thermo Fisher Scientific) supplemented with 10% BSA (093001 ...
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bioRxiv - Cancer Biology 2022Quote: ... T cells were then resuspended at 2 × 106 cells/ml in PBS and stained with Cell Trace Violet (1:2000, Invitrogen) for 20 minutes at 37°C ...
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bioRxiv - Cancer Biology 2022Quote: ... before co-culture T cells were resuspended 1×106 cells/mL in 1X PBS containing Celltrace Violet Cell (cat#C34557 from Thermofisher) for 20 minutes at 37°C ...
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bioRxiv - Bioengineering 2022Quote: Murine MC38-RFP adenocarcinoma cells and MC38 hybrid cells (MC38-macrophage hybrid cells)8 were cultured in Dulbecco’s modified eagle medium (DMEM) (Thermo Fisher) supplemented with 10% FBS ...
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bioRxiv - Immunology 2022Quote: ... protein was evaluated in lysate cells and the type of cell death was evaluated in detached cells after treatment with trypsin (Gibco). For treatment experiment ...
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bioRxiv - Cell Biology 2023Quote: ... GFP and FLPo cells were trypsinized and the cell concentration was determined using a Countess Automated Cell Counter (Thermofisher, USA). The final concentration was the average of three reads from 3 different aliquots of cell suspension ...
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bioRxiv - Immunology 2024Quote: ... Red blood cells were lysed in single-cell suspension of bone marrow cells using ACK Lysing Buffer (Thermo Fisher Scientific) and granulocytes were separated from mononuclear cells using 70%/86% Percoll gradient at interphase ...
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Single-cell profiling and zebrafish avatars reveal LGALS1 as immunomodulating target in glioblastomabioRxiv - Cancer Biology 2023Quote: ... a single-cell suspension was created by treating the GBM cells for 3 minutes with accutase (StemPro Accutase Cell Dissociation Reagent, Gibco) at 37°C.
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bioRxiv - Cell Biology 2023Quote: ... Cell division kinetics were carried out by tracking cell divisions of single sorted cells into 60-well plates (ThermoFisher Scientific) for 3 days post-sort ...
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bioRxiv - Cell Biology 2023Quote: ... Adherent cell lines were maintained by first rinsing cells with 1x PBS and then treating cells with TrypLE (ThermoFisher Scientific) for a maximum of 5 minutes at 37℃ before quenching the reaction with complete media ...
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bioRxiv - Cancer Biology 2023Quote: PD-L1 expression on tumor cells: A549 cells incubated with stimulated T cells were also analyzed for PD-L1 expression (APC, Invitrogen) and viability with 7AAD staining.
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bioRxiv - Cancer Biology 2023Quote: ... Cell viability of A2780 cells after CDDP treatment was evaluated by the Live/Dead Cell Stain Kit (InvitrogenTM, ThermoFisher Scientific) by flow cytometry (BD FACS CantoII ...
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bioRxiv - Cell Biology 2023Quote: ... Cells were filtered through a 100 µm cell strainer and red blood cells were lysed using ACK buffer (A1049201, Gibco). Cells were washed in PBS ...
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bioRxiv - Cell Biology 2023Quote: The following antibiotics were used to select cell lines: Puromycin (1 μg/ml for HeLa cells and μg/ml for HEK293T cells; A1113803; Gibco) and Blasticidin (15 μg/ml for HeLa cells and 5 μg/ml for HEK293T cells ...
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bioRxiv - Cancer Biology 2023Quote: ... We generated two LTED variants A and B cells from their corresponding parental cell lines by chronically passaging parental cell lines in phenol red-free IMEM media (Gibco) supplemented with 10% charcoal-stripped bovine serum (CSS ...
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bioRxiv - Biochemistry 2023Quote: ... and used to infect Sf21 insect cells at a cell density of 1.0 x 106 cells/mL in Sf-900 SFM medium (Gibco Life Technologies), shaking at 90 rpm on Sartorius Stedim Biotech “CERTOMAT RM” with a 25mm orbit ...
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bioRxiv - Physiology 2024Quote: ... The total cell count (comprising both viable and non-viable cells) was determined utilizing an automated cell counter (Countess, ThermoFisher).
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bioRxiv - Immunology 2024Quote: Single cell suspensions were obtained by detaching the cells from the culture plates using StemPro Accutase Cell Dissociation Reagent (Gibco) according to the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2024Quote: ... Cells were re-suspended at a density of 1×106 cells/ml in cell staining solution (Vybrant CM-Dil cell labelling solution (Invitrogen) diluted 1:250 in Optimem [Gibco] ...
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bioRxiv - Immunology 2024Quote: ... The infected and transduced Lin− cells were cultured and differentiated into B cells on OP9 cells in IMDM (Thermo Fisher) supplemented with 10% fetal bovine serum ...
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bioRxiv - Cell Biology 2024Quote: ... U2-OS cells were trypsinized and cell density was determined using a Countess II Automated Cell Counter (Thermo Fisher Scientific). 2 × 103 U2-OS cells were seeded in each well of a 384-well plate in 32 µl complete culture medium ...
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bioRxiv - Genetics 2024Quote: ... Cells were seeded at a density of 4 × 106 cells per 10-cm BioLite Cell Culture dish (Thermo Fisher Scientific) and transfected the next day using Opti-MEM (Gibco ...
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bioRxiv - Cell Biology 2020Quote: ... Each labeled sample was assayed with the Affymetrix PrimeView human gene expression array read with an Affymetrix GeneChip Scanner 3000 (Affymetrix/Thermo Fisher Scientific, Waltham, MA, USA).
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Dual signaling via interferon and DNA damage response elicits entrapment by giant PML nuclear bodiesbioRxiv - Microbiology 2021Quote: ... cells were isolated from human foreskin tissue and cultivated at 37 °C and 5% CO2 in Eagle’s minimal essential medium (Gibco, Thermo Fisher Scientific Inc., Waltham, MA, USA) containing 7 % fetal calf serum (FCS ...
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bioRxiv - Neuroscience 2020Quote: The gene expression data for the human cerebral cortex used in this study is downloaded from the NCBI Gene Expression Omnibus under the accession number GSE25219 (Affymetrix Human Exon 1.0 ST Array)(43) ...
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bioRxiv - Cancer Biology 2020Quote: Human CD34+ cells were isolated from patient bone marrow samples by erythrocyte lysis buffer treatment, followed by separation with AutoMACs sorter and CD34 Microbead Kit human (MiltenyiBiotech, Germany) and grown in IMDM (Gibco, Thermo Fisher Scientific, CA, USA) supplemented with 2% FBS (Gibco ...
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bioRxiv - Cell Biology 2021Quote: ... Human islets were purified using Ficoll density gradients [31] and cultured in Connaught Medical Research Laboratories (CMRL) 1066 medium (Invitrogen, Life Technologies, Carlsbad, CA, USA) supplemented with 10% fetal calf serum ...
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bioRxiv - Cancer Biology 2022Quote: ... Cas9 over expressing cells were transduced with lentiviruses expressing gRNA with no sequence homology to any region of the human genome (LentiArray™ CRISPR Negative Control Lentivirus, Thermo Fisher Scientific, Cat # A32327).
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... Epi-Life® Growth Medium with 60 µM calcium and Cascade Biologist human keratinocyte growth supplement (HKGS) 100X (S-001–5) were from Life Technologies (Grand Island, NY, USA). The Dulbecco’s phosphate-buffered saline (DPBS) ...
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bioRxiv - Immunology 2022Quote: ... operated by UNICORN 6.4, using HiTrap Protein A columns (Cytiva, catalog no. GE17-5079-01) for human IgG1 and CaptureSelect™ CH1-XL (Thermo Fisher, catalog no. 494346201) for Fab fragments ...
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bioRxiv - Molecular Biology 2020Quote: ... Expression profiling of the kidney biopsies was previously carried out using Affymetrix GeneChips (HumanGenome U133 Array and U133Plus2 Array and Affymetrix Human Gene ST GeneChip 2.1) as reported (35,36) ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... parasites were maintained in vitro essentially as described previously,12 in human O- erythrocytes suspended in HEPES- and GlutamaxTM-supplemented RPMI 1640 (Thermo Fisher Scientific, order number 72400120) to which 11 mM D-glucose ...
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bioRxiv - Microbiology 2020Quote: ... S100 Calcium-binding protein A7 (S100A7) and human β-defensin 2 (DEFB4A) were amplified by using TAQMAN Gene Expression Assays (Applied Biosystems, Foster City, CA). TaqMan probes were grafted with a fluorophore (FAM ...
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bioRxiv - Developmental Biology 2020Quote: ... the ells were transfected with human EC-SOD (hEC-SOD) cDNA inserted into a vector plasmid pcDNA3 (5446 nucleotides; Invitrogen Life Technologies, Carlsbad, CA, USA) as previously described,14 using the FuGENE kit (Roche Diagnostics ...
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bioRxiv - Immunology 2020Quote: ... Immune mediator levels in COVID-19 patient plasma samples across acute samples were measured with by Luminex using the Cytokine/Chemokine/Growth Factor 45-plex Human ProcartaPlex™ Panel 1 (ThermoFisher Scientific, #EPX450-12171-901). Data acquisition was performed on FLEXMAP® 3D (Luminex ...
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bioRxiv - Immunology 2021Quote: ... with an N-terminal IL-2 signal peptide for secretion and a Fc part (hinge-CH2-CH3) of human IgG1 for purification was inserted into pFUSE-mIgG1-Fc2 vector (Invitrogen, Thermo Fisher Scientific, MA, USA). The construct (RBD-huFc ...
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bioRxiv - Neuroscience 2023Quote: ... and growth factors was performed using a customized multiplex bead-based immunoassay - Immune Monitoring 65-Plex Human ProcartaPlex™ Panel (#EPX650-10065-901; ThermoFisher Scientific, Waltham, MA, US) according to manufacturer’s instructions ...
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bioRxiv - Pathology 2024Quote: ... primary dermal fibroblasts (normal, human, adult; PCS-201-012, ATCC, VA, USA) were cultured in Dulbecco’s Modified Eagle medium (DMEM) (Thermo Fisher Scientific Inc., Waltham, MA, USA) supplemented with 10% fetal bovine serum (FBS ...
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bioRxiv - Immunology 2024Quote: The amounts of TNF-α and IL-6 in culture supernatants of LPS or Pam3cys-re-stimulated IS-trained macrophages were quantified using commercial human ELISA kits (Thermo Fisher Scientific, Waltham, MA, USA). Optical density was measured using the Infinite M200 (Tecan ...
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bioRxiv - Immunology 2022Quote: ... from normal donors were obtained with an NIH-approved protocol and activated with CD3 and CD28 microbeads at a ratio of 1:3 (Dynabeads Human T-Expander CD3/CD28, Thermo Fisher Scientific, catalog no. 11141D) in AIM-V media containing 40 IU/mL recombinant IL-2 and 5% FBS for 24 hr ...
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bioRxiv - Neuroscience 2023Quote: ... which specifically targets on the phosphorylated Thr188 of murine C/EBPβ (a conservative locus to Thr235 of human C/EBPβ) in eBioscience™ Transcription Factor Staining Buffer Set (Invitrogen, Catalog #00-5523-00). For background signal staining ...
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bioRxiv - Cancer Biology 2023Quote: ... supplemented with 5% fetal bovine serum except for RWPE-1 and RWPE-2 cell lines which were grown in a keratinocyte serum-free medium supplemented with 5 ng/mL recombinant human epidermal growth factor (EGF) and 50 μg/mL bovine pituitary extract (Gibco™, Invitrogen, Carlsbad, CA, USA). The cell lines were tested for short tandem repeat (STR ...
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bioRxiv - Cell Biology 2023Quote: NHK/38 cells and sections of normal human epidermis were stained with a monoclonal anti-hIL-38 antibody (H127C) (14-7385-82, Thermo Fisher Scientific AG, 1/2000), followed by an Alexa Fluor 594-labeled goat anti-mouse IgG2b (115-585-207 ...
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bioRxiv - Genomics 2023Quote: ... 5 U/mL, human Chorionic Gonadotropin-hCG (Chorulon®, Intervet International B. V., Boxmeer, Netherland) and 50 μg/mL gentamicin (Gibco Thermo Fisher, Massachusets - USA). Culture droplets were covered with mineral oil and placed in an incubator at 38.5°C and a humid atmosphere of 5% CO2 in atmospheric air ...
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bioRxiv - Immunology 2023Quote: ... were seeded in Ultraculture complete medium and partially activated with dynabeads human T-activator CD3/CD28 (final dilution 1:1000) (Thermo Fisher Scientific, Waltham, MA USA) or completely activated with dynabeads human T-activator CD3/CD28 and IL2 2.5 U/ml ...
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bioRxiv - Neuroscience 2024Quote: ... The expression of specific proteins in NDEs were quantified by ELISA kits for human P-serine 312-IRS-1 (Life Technologies Corporation, Carlsbad, CA, USA), human P-pan-tyrosine-IRS-1 (Cell Signaling Technology ...