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Citations for Thermo Fisher :
8401 - 8450 of 10000+ citations for Killer cell immunoglobulin like receptor 2DL3 KIR2DL3 Human HEK293 His since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2024Quote: ... coli TOP10 cells (Invitrogen), and plated onto agar plates with 100 µg/mL carbenicillin and 0.2% arabinose ...
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bioRxiv - Biochemistry 2024Quote: Expi293F suspension cells (ThermoFisher) were maintained at 0.4x106 cells.mL-1 twice a week in FreeStyle™ 293 Expression Medium (ThermoFisher ...
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bioRxiv - Developmental Biology 2020Quote: ... Single cell suspensions were then evaluated for viability and cell count by using a Countess II FL Automated Cell Counter (Invitrogen, ThermoFisher). Cells were then diluted to a concentration of 600 cells/µl for a target cell range of ∼5000 cells to be individually encapsulated into nanoliter-scale droplets containing reverse transcription reagents and uniquely barcoded Gel Bead-in EMulsions (GEMS) ...
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bioRxiv - Biochemistry 2021Quote: ... The number of total and dead cells was determined at specified time points by co-staining cells with Hoechst 33342 (to identify all cells, Life Technologies) and propidium iodide (to identify dead cells ...
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bioRxiv - Microbiology 2020Quote: ... Cells were transformed in BL-21 cells and IE86-FL-pMALcx5 transformed cells were grown in 1L luria broth (Thermofisher Scientific) containing ampicillin (Sigma-Aldrich ...
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bioRxiv - Cancer Biology 2020Quote: ... MCF7 (4000 cells/well) and HCC70 (4500 cells/well) breast cancer cells were plated in 100 µl of DMEM culture medium (Life Technologies) in 96-well plates (TPP 96 well tissue culture plates from Sigma-Aldrich ...
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bioRxiv - Immunology 2020Quote: Single cell suspension of mouse spleens were prepared by passing cells through 70 μm cell strainers and ACK lysis (Thermo Fisher) prior to resuspension in complete media (αMEM supplemented with 10% FCS ...
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bioRxiv - Immunology 2020Quote: ... and 1 μg DNA per 1 mL cell culture medium at a cell density of 0.8 106 cells/mL in FreeStyle 293 medium (Thermo Fisher Scientific). After 7 days of culture at 37°C and 5% CO2 ...
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bioRxiv - Immunology 2021Quote: ... cells were washed with PBS before being harvested in cells-to-cDNA Cell Lysis Buffer (Ambion Inc, Foster City, CA, USA), and transferred to a PCR plate for cells-to-cDNA synthesis ...
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bioRxiv - Bioengineering 2022Quote: Co-culture cell viability and cell death was measured using the LIVE/DEAD™ Cell Imaging Kit (488/570) (Thermofisher Scientific) according to the manufactorers protocol ...
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bioRxiv - Immunology 2022Quote: ... A viability staining was then performed on the positive fraction containing tetramer-enriched cells by incubating cells with 100μL of LIVE/DEADTM Fixable Aqua Dead Cell Stain kit (ThermoFisher Scientific #L34957) for 15min at 4°C ...
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bioRxiv - Molecular Biology 2022Quote: ... cells were FACS sorted directly into 5 or 10μl Single cell lysis solution (SCLS; Single cell lysis kit Invitrogen, cat# 4458235) with DNase I according to manufacturer’s instructions with the exception that the lysis reaction was incubated for 15min at room temperature (RT ...
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bioRxiv - Immunology 2023Quote: ... DCs and CD4+ T cells were washed and resuspended in RPMI supplemented with 2% FCS at a cell density of 1×107 cells/ml and labelled with 7.5μM Cell Tracker Red CMTPX (Thermofisher; Waltham, MA, USA) or 7.5μM vibrant CFDA SE (CFSE ...
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bioRxiv - Neuroscience 2024Quote: Intracellular ROS in BV-2 cells was measured by incubating cells with 1 µM cell-permeant 2’,7’-dichlorodihydrofluorescein diacetate (H2DCFDA, Invitrogen, D399) at 37°C for 30 min.
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bioRxiv - Immunology 2024Quote: ... These cells were cultured with purified OVA-specific CD4+ or CD8+ T cells that were stained with a cell proliferation dye (Cell Trace Violet, ThermoFisher Scientific) for three days (100,000 cells/well) ...
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bioRxiv - Microbiology 2023Quote: ... bacmids were isolated by isopropanol precipitation and used to transfect Sf9 cells at a cell density of 1 million cells/ml in Sf-900™ II serum-free media (Gibco). First generation baculoviruses (P1 ...
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bioRxiv - Developmental Biology 2023Quote: ... 50 cells were sorted into 10 μL of Single cell lysis buffer supplemented with DNAseI (Single Cell-to-CT Kit ThermoFisher, 4458237) and stored at -80°C ...
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bioRxiv - Immunology 2023Quote: Spleen cells were prepared for intracellular staining as described under “Flow cytometry and cell sorting” with the following modifications: cells were stained with the Live/Dead Fixable Aqua Dead Cell Stain Kit (Invitrogen, L34966) for 30 min at 4°C (1:500 dilution in PBS ...
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bioRxiv - Immunology 2023Quote: ... cell pellets were re-suspended and red blood cells were removed using Red Blood Cell Lysis Buffer (Thermo Fisher Scientific, A1049201). The total cell count was determined using a cell counting chamber under light microscopy ...
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bioRxiv - Pathology 2023Quote: ... cells were counted and critically assessed for single cell separation and overall cell viability using the Countess 3 FL (Fisher Scientific). Samples were then stained for multiplexing using cell hashing [29] ...
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bioRxiv - Cell Biology 2023Quote: ... First, cell detachment was attained using a cell dissociation solution (at 2:2:1 ratio, Cell Dissociation Buffer (Thermo Fisher Scientific): RPMI 1640 ...
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bioRxiv - Developmental Biology 2023Quote: ... The cell pellet was washed twice and lyse the cells with RIPA cell lysis and extraction buffer (Thermo Scientific™, 89900) and 1x Protease Inhibitor cocktail (PIC ...
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bioRxiv - Biochemistry 2023Quote: ... and used to infect Sf21 insect cells at a cell density of 1.0 x 106 cells/mL in Sf-900 SFM medium (Gibco Life Technologies), shaking at 90 rpm on Sartorius Stedim Biotech “CERTOMAT RM” with a 25mm orbit ...
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bioRxiv - Microbiology 2023Quote: ... Percentage of mast cells among the peritoneal cell population was determined after staining an aliquot of cells with Hema3 kit (Fisher Scientific).
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bioRxiv - Microbiology 2024Quote: ... cells were seeded at 50,000 cells per well in 12 well plates by detaching cells using StemPro Accutase Cell Dissociation Reagent (Thermo Fisher Scientific), and seeding in plates coated with Matrigel (Sigma) ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... was used to detach the cells during passage and the number of live cells was calculated with an automatic cell counter (Countess II, Thermo Fisher) after staining the cells with trypan blue (Sigma ...
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bioRxiv - Pathology 2024Quote: Total cellular RNA was extracted from sorted lymphocyte samples (20,000-200,000 cells/sample) or single cell suspensions (108 cells) using TRIzol® Reagent (Invitrogen #15596-018). Total RNA was purified using the RNeasy MinElute Cleanup Kit (Qiagen #74204) ...
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bioRxiv - Cancer Biology 2024Quote: ... single-cell suspensions were stained with Hoechst (staining of all cells) and propidium iodide (staining of dead cells) (Thermo Fisher Scientific) to distinguish live and dead cells according to the manufacturer’s specifications ...
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bioRxiv - Cancer Biology 2024Quote: Mononuclear cells isolated from the LLC1 single-cell suspension were stained with Live/dead fixable green dead cell stain (Invitrogen, L23101) and fluorochrome-labeled monoclonal antibodies ...
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bioRxiv - Immunology 2021Quote: To investigate a link between fkbpl gene expression and psoriasis a search was performed on the NCBI GEO database to obtain mRNA microarray results and identified GSE14905 from skin biopsy samples (Affymetrix Human Genome U133 Plus 2.0 Array) which compared a non-disease control cohort and a diseased cohort ...
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bioRxiv - Immunology 2022Quote: ... samples were resuspended in binding buffer containing a 1:100 dilution of mouse anti-human-IgG1-Fc conjugated to Alexa Fluor 488 (Thermo Fisher Scientific, Rockford, IL; A-10631) and incubated for 30 minutes at room temperature in the dark ...
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bioRxiv - Neuroscience 2022Quote: ... were incubated with 100 ng/μL of mouse anti-human CD171 (L1CAM, neural adhesion protein) biotinylated antibody (clone 5G3, eBioscience/Thermo Fisher Scientific; Catalog # 13-1719-82); mouse anti-human GLAST (ACSA-1 ...
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bioRxiv - Bioengineering 2024Quote: Engineered cardiac tissues and hiPSC-cardiomyocytes cultured with patient IgG were fixed and incubated with secondary anti-human IgG (Thermo Fisher Scientific, cat. no. A-11013) to visualize cellular autoantibody targets ...
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bioRxiv - Molecular Biology 2023Quote: ... Evaluation of proliferation in tissues using antibodies against Ki67 or BrdU (anti-human Ki67 and anti- BrdU monoclonal antibodies; Thermo-Fisher Scientific and BD BioSciences, USA, respectively) was performed as previously described {GarciaMelendrez:GKcY05su } ...
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bioRxiv - Cell Biology 2023Quote: ... HUVECs were grown to ∼80% confluence followed by transfection with 100 nM human ERG Silencer Select (ID #s4811) or nontargeting siRNA oligos (Life Technologies: ERG #4392420 and nontargeting #AM4635) using lipofectamine RNAiMAX (Life Technologies ...
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bioRxiv - Immunology 2023Quote: ... recombinant human IL-3 (R&D, Cat. 203-IL-050/CF, 25 ng mL-1) or c) human M-CSF recombinant protein (Invitrogen, Cat. PHC9501, 100 ng mL-1). A partial medium change was performed every 48 hours with 2x cytokine composites to replenish cytokines ...
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bioRxiv - Cancer Biology 2023Quote: ... The primary anti-human CD38 antibody was applied at a dilution of 1:250 and incubated overnight at 4°C (Thermo Scientific clone RM388 Cat# MA5-36061). After washing with PBS ...
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bioRxiv - Molecular Biology 2024Quote: Human fibroblasts were seeded in a 24-well plate and stimulated with tumor necrosis factor-α (TNF-α) (5 ng/ml; Life Technologies, Landsmeer, the Netherlands) plus lymphotoxin α1β2 (50 ng/ml ...
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bioRxiv - Cancer Biology 2024Quote: ... cells were transduced with lentiviruses expressing Sg RNA with no sequence homology to any region of the human genome (LentiArray™ CRISPR Negative Control Lentivirus, Thermo Fisher Scientific, Cat No# A32327). Vehicle-treated knockouts were normalized to vehicle-treated negative control Sg RNA whereas ...
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bioRxiv - Cancer Biology 2021Quote: ... Cell viability was measured using alamarBlue Cell Viability Reagent (DAL1100, Thermo Fisher Scientific) according to the manufacturer’s instructions.
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bioRxiv - Molecular Biology 2020Quote: Mouse ES cells were dissociated into single cells by incubation with TrypLE (Gibco). The ells were washed and diluted in PBS ...
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bioRxiv - Cancer Biology 2021Quote: ... Seahorse XF Cell culture microplates were treated with Cell Tak (Thermo Fisher Scientific) according to the manufacturer’s instructions and number of viable cells was determined using Countess Automated cell coulter with trypan blue stain before plated in 22 or more replicates at a density of 25000 cells/ well ...
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bioRxiv - Cell Biology 2020Quote: ... Cells were transferred into cell culture media or Hanks’ balanced salt solution (Invitrogen) with 10 mM HEPES (Sigma-Aldrich ...
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bioRxiv - Cell Biology 2020Quote: ... 1×106 cells were plated on cell culture inserts (140640, Thermo Fisher scientific), and 4 h later unadherent cells were removed by medium change ...
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bioRxiv - Cell Biology 2020Quote: Cell number was counted with a Countess II FL Automated Cell Counter (ThermoFisher) at indicated time intervals ...
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bioRxiv - Cell Biology 2020Quote: ... Whenever cells were dissociated into single cells using 0.5 × TrypLE Select (Thermo Fisher) or Accumax (Sigma Aldrich) ...
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bioRxiv - Immunology 2021Quote: ... T cells were labelled with the CellTrace CFSE Cell Proliferation Kit (C34554 ThermoFisher) following manufacturer’s instructions ...
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bioRxiv - Cell Biology 2020Quote: ... The cells were then counted using a Countess II cell counter (Thermo Fisher), and the number of Trypan-positive cells recorded ...
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bioRxiv - Immunology 2021Quote: ... T-cells were labeled with Cell Trace Violet (CTV) dye (Thermo Fisher Scientific) for 20 min at 37°C at a final concentration of 2.5 μM in PBS ...
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bioRxiv - Immunology 2021Quote: ... T-cells were labeled with Cell Trace Violet (CTV) dye (Thermo Fisher Scientific) for 20 min at 37°C at a final concentration of 2.5 μM in PBS ...