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Citations for Thermo Fisher :
8151 - 8200 of 10000+ citations for Human UFL1 shRNA Plasmid since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2023Quote: ... we initially digested the pNL4-3 plasmid with EcoRI and XhoI and subcloned this fragment into pcDNA3.1/myc-His A (Invitrogen). To generate pcDNA3.1 NL4-3 Env TMTR ...
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bioRxiv - Molecular Biology 2023Quote: ... cells were transfected with 2-5 µg of the indicated plasmid using Lipofectamine 3000 as per manufacturer’s instructions (ThermoFisher). Transfection medium was exchanged to fresh medium and cells were moved to 10 cm plates 4-6 hours post-transfection ...
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bioRxiv - Immunology 2023Quote: ... Expi293F cells were co-transfected with heavy and light chain plasmid using Expifectamine (Thermo Fisher Scientific, Cat No. A14526) per the manufacturer’s protocol ...
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bioRxiv - Microbiology 2023Quote: ... CHIK1 and GFP sequences cloned in the T444T plasmid was transcribed in vitro using the T7 MEGAscript Kit (Invitrogen) following the manufacturer’s instructions ...
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bioRxiv - Developmental Biology 2023Quote: ... 4 colonies were selected for further culturing before cell lysis and plasmid DNA purification using MiniPrep Kit (Thermo Scientific).
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bioRxiv - Biophysics 2023Quote: ... Recombinant bacmids were generated from the plasmids in Table S1 using MAX Efficiency DH10Bac competent cells (Gibco; Cat. 10361012) and transfected into Sf9 insect cells (Gibco ...
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bioRxiv - Cancer Biology 2023Quote: ... Cells were plated onto 13-mm coverslips in 35-mm dishes and transfected at 40% confluency with 1.6 μg of plasmid using Lipofectamine 2000 (Invitrogen).
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bioRxiv - Cell Biology 2023Quote: ... The cells were transfected with plasmid DNAs (1.0 μg each) using Lipofectamine 2000 reagent (11668-019; Thermo Fisher Scientific). After washing with HBSS (14025076 ...
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bioRxiv - Molecular Biology 2023Quote: ... The insertion TRIP12 cDNA and its IDR into the pDest-RFP-C1 plasmid was performed using Gateway strategy (Invitrogen) to generate the pDest-RFP-TRIP12 and pDest-RFP-IDR plasmids.
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bioRxiv - Microbiology 2023Quote: ... Heavy and light cloned plasmids were mixed 1:1 and transfected into Expi293 cells according to manufacture protocol (ThermoFisher). Supernatants were harvested five days later and purified on protein A/G Hi-Trap columns on an AKTA FPLC (Cytiva) ...
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bioRxiv - Microbiology 2023Quote: HeLa or EA.hy926 cells grown to 90% confluency were transfected with plasmid DNA using Lipofectamine 2000 (Invitrogen, Carlsbad, CA) and incubated at 37 °C in a humidified incubator at 5% atmospheric CO2 for 18 to 24 h ...
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bioRxiv - Microbiology 2023Quote: ... the whole plasmid was amplified by using the Thermo Scientific Phusion High-Fidelity DNA-Polymerase (Thermo Fisher Scientific Inc.). Constructs of pDolos were generated with the Gibson assembly method 41 ...
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bioRxiv - Molecular Biology 2023Quote: ... the pc3.1-GFP-tubulin plasmid was transfected into U2OS cells under the standard protocol of Lipofectamine 3000 (L3000, Invitrogen). Briefly ...
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bioRxiv - Molecular Biology 2023Quote: ... The mRNAs for these constructs were synthesized from linearized plasmids using the mMessage mMachine SP6 Kit (Thermo Fisher Scientific). All synthesized RNAs were purified using the RNeasy Mini Kit (Qiagen ...
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bioRxiv - Molecular Biology 2023Quote: 8–10 ng of plasmid pools (pHPSC1142, pHPSC1160, pHPSC1117) were used in PCR using Phusion polymerase (Thermo Fisher F530S) or Phusion Flash High-Fidelity PCR Master Mix (Thermo Fisher F548L) ...
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bioRxiv - Microbiology 2023Quote: ... cells were transfected with 2 μg of plasmid encoding WT S or the indicated mutant using Lipofectamine 2000 (Invitrogen) following the manufacturer’s protocol ...
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bioRxiv - Cell Biology 2023Quote: ... wild type HEK293 or HEK293A (for figure 1A) cells were transfected with the indicated plasmids with Lipofectamine2000 Reagent (Invitrogen) as per manufacturer protocol ...
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bioRxiv - Cell Biology 2023Quote: ... Cells were transfected with plasmids carrying the sgRNAs and were incubated in DMEM supplemented with 15% FBS (Gibco, 10437028), 100 U/ml penicillin and streptomycin ...
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bioRxiv - Biochemistry 2023Quote: ... 1.0 x 106 Sf9 cells were co-transfected with 1.5 μg plasmid DNA and 500 ng linearized Bac10:KO1629 viral DNA using Cellfectin II (Invitrogen) following the manufacturer’s protocol ...
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bioRxiv - Developmental Biology 2024Quote: ... Cas9 RNA was transcribed from the pCS2-nCas9n plasmid (57) using the mMESSAGE mMACHINE™ SP6 Transcription Kit (Invitrogen). sgRNA and Cas9 RNA were diluted in 1x Danieau’s buffer (5mM HEPES (pH 7.6) ...
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bioRxiv - Cell Biology 2024Quote: ... we cloned the zact sequence into the bacterial plasmid pCR 2.1 (TOPO TA cloning kit with One Shot TOP10 chemically competent cells, Invitrogen). TOP10 chemically competent cells were also used in other steps.
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bioRxiv - Biochemistry 2024Quote: ... Cells were grown to 70% confluency in T75 flasks prior to transient transfection with 10 µg of Cdc20_HiBiT_pcDNA3.1(-) plasmid with Lipofectamine 2000 (Invitrogen, ThemoFisher Scientific) according to the manufacturers’ protocol ...
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bioRxiv - Biochemistry 2024Quote: ... Plasmids were introduced into U2OS cells by electroporation using the Neon™ Transfection System 10 μL Kit (ThermoFisher Scientific) and cells seeded on eight-well microscopy slides (Ibidi ...
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bioRxiv - Neuroscience 2024Quote: ... The DNA was amplified using the PureLink™ Expi Endotoxin-Free Maxi Plasmid Purification Kit (Thermo Fisher Scientific, A31231) and the lentiviral production was carried out by iVector facility of the Paris Brain Institute under BioSafety Level 2 (BSL-2 ...
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bioRxiv - Plant Biology 2024Quote: ... To generate GreenGate compatible entry clones we used collection compatible entry plasmids or CloneJET PCR Cloning Kit (Thermo Scientific) according to the manufacturer manual ...
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bioRxiv - Cancer Biology 2024Quote: ... typically 1.5 μg of plasmid was reverse transfected into 300,000 293T cells in a well of a 6-well plate using OptiMEM (Gibco) and FuGENE 6 (Promega) ...
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bioRxiv - Cell Biology 2024Quote: ... Plasmids were extracted from the selected colonies and sequence confirmed through sequencing with M13 primers (Invitrogen; N52002 and N53002).
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bioRxiv - Biochemistry 2024Quote: ... 100 ng dual-fluorescent reporter plasmids with various concentrations of PNAs were co-transfected into cells using lipo3000 (Invitrogen) according to the instructions ...
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bioRxiv - Cell Biology 2024Quote: ... The hcMEC / D3 cells were transfected with HBA1 RNAi plasmid mixed with LipofectamineTM 3000 and P3000TM (Invitrogen / Thermo Fisher) in advance for 24 hours ...
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bioRxiv - Cell Biology 2024Quote: ... The hcMEC / D3 cells were transfected with HBA1 RNAi plasmid mixed with LipofectamineTM 3000 and P3000TM (Invitrogen / Thermo Fisher) in advance for 24 hours ...
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bioRxiv - Microbiology 2024Quote: ... the sequence of the resulting recombinant plasmid was ascertained by Sanger sequencing techniques (Applied Biosystems™ 3130 Genetic Analyser), and the resulting data were decoded by ApE software (v2.0.36 ...
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bioRxiv - Biochemistry 2024Quote: ... CENP-Ufl and 115-C constructs were cloned into pcDNA5 plasmids (75) and were co-transfected with pOG44 (Invitrogen), encoding the Flp recombinase ...
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bioRxiv - Microbiology 2024Quote: ... grown at 37°C and 8% CO2 were prepared and transfected with plasmids according to the manufacturers protocol (ThermoFisher). Briefly ...
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bioRxiv - Molecular Biology 2024Quote: ... This allowed precise positioning of subunits into the pTB207 plasmid using an appropriate mix of FastDigest (ThermoFisher Scientific, USA) restriction enzymes ...
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bioRxiv - Immunology 2024Quote: ... 250 ng plasmids encoding SARS-CoV-2 spike mutants or ACE2 variants were packaged in Lipofectamine 2000 (Life technologies) and transfected for 24 hours ...
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bioRxiv - Neuroscience 2024Quote: ... The pNL4-3 and EcoHIV/NDK plasmid amplification was accomplished using Stbl3 competent cells (Thermo Fisher Scientific, Cat# C737303). A total of 50 µg of proviral plasmid was transfected into 107 HEK293T/17 cells for virus production using Lipofectamine 2000 (Thermo Fisher Scientific ...
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bioRxiv - Molecular Biology 2024Quote: ... This plasmid was used to prepare baculovirus according to standard protocols (Bac-to-Bac Baculovirus Expression System, Thermo Fisher). Detection of gp64 was used to determine baculovirus titer (Expression Systems) ...
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bioRxiv - Molecular Biology 2024Quote: ... This plasmid was used to prepare baculovirus according to standard protocols (Bac-to-Bac Baculovirus Expression System, Thermo Fisher). For expression ...
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bioRxiv - Biochemistry 2024Quote: ... 0.6 μg 3xMyc-Ubiquilin was co-transfected with 5.4 μg pOG44 at a 9:1 ratio of pOG44 to plasmid in Opti-MEM (Gibco) using 3 μL Lipofectamine 2000 (Invitrogen) ...
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bioRxiv - Cell Biology 2024Quote: ... MDA-MB-231 cells were co-transfected with pXPR_001-sgRab21 and pUC57-Rab21 HDR plasmids in 1:4 ratio using Lipofectamine 3000 (Invitrogen). Five days post-transfection ...
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bioRxiv - Neuroscience 2024Quote: ... Cells were plated at a density of 5.7 × 105 cells per well in a 6-well plate overnight in complete media before adding 1 μg of plasmid DNA and incubating with 3.5 μL Lipofectamine 2000 (ThermoFisher) for 20 min ...
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bioRxiv - Developmental Biology 2024Quote: ... Subsequently, the plasmids were recombined into the pAW vector (DGRC, stock 1127) using Gateway LR-clonase II (Thermo Scientific). For the ExoA-mCherry-nGFP plasmid ...
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bioRxiv - Genetics 2024Quote: ... and the resulting entry vector was recombined into the plasmid p2GW7L using Gateway LR Clonase II (Thermo-Fisher Scientific). To generate the constructs pMBR1:MBR1 and pMBR1wet:MBR1wet ...
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bioRxiv - Cancer Biology 2024Quote: Lentiviruses were generated by co-transfecting lentivector plasmids of interest together with packaging vectors pCMVR8.74 and pMD2.G using Opti-MEM (Gibco) and Lipofectamine 2000 reagent (Invitrogen ...
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bioRxiv - Cell Biology 2024Quote: ... we purified the sgRNA construct and the repair template plasmids with the PureLink Quick PCR purification Kit (Invitrogen, #K310001) and co-injected them into N2 animals with the pRF4 [rol-6 (su1006)] and Podr-1::dsRed selection markers ...
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bioRxiv - Molecular Biology 2024Quote: ... to 70% confluence were transfected with 3.5µg of pAm-Cyan and Wt-LC-pAm-Cyan plasmids (Barrera-Vázquez et al., 2019) using Lipofectamine 2000 (Thermofisher) in MEM medium (Gibco ...
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bioRxiv - Molecular Biology 2024Quote: ... Purification of plasmids and PCR products was performed using commercially available kits (Thermo Fisher Scientific and A&A Biotechnology). Transformation of E ...
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bioRxiv - Immunology 2024Quote: ... The two plasmids were co-transfected into Expi293 cells using ExpiFectamine293™ transfection reagents (Thermo Fisher Scientific, Waltham, MA), following manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2020Quote: ... pcDNA-TruB1 was generated by inserting the ORF of human TruB1 with a Flag sequence into pcDNA.3.1(+) (Thermo Fisher Scientific) at the Nhe1 and EcoR1 sites ...
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bioRxiv - Cell Biology 2019Quote: ... and SqCC/Y1 cells and primary keratinocytes were routinely maintained in EpiLife medium (with 60 mM calcium concentration) (MEPI500CA) supplemented with Human Keratinocyte Growth Supplement (HKGS; ThermoFisher Scientific). For the experiments ...