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Citations for Thermo Fisher :
7951 - 8000 of 10000+ citations for Human UFL1 shRNA Plasmid since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2020Quote: ... was produced in ExpiCHO cells by transfection of 6 x106 cells/ml at 37 °C with 0.8 □g/ml of plasmid DNA using the ExpiCHO expression system transfection kit in ExpiCHO Expression Medium (ThermoFisher). One day later the cells were refed ...
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bioRxiv - Microbiology 2020Quote: Antibody heavy and light plasmids were co-transfected at a 1:1 ratio into HEK293F cells (Thermo Fisher Scientific) using PEI Max 40K (linear polyethylenimine hydrochloride ...
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bioRxiv - Microbiology 2021Quote: ... and 500 ng of plasmids expressing parental S or its derivatives using Lipofectamine 3000 (Thermo Fisher Scientific, Cat# L3000015) or PEI Max (Polysciences ...
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bioRxiv - Cancer Biology 2020Quote: ... mOrange reporter gene) along with non-fluorescent carrier plasmid (700 ng) was transfected into cells using Lipofectamine 3000 (ThermoFisher) according to the manufacturer’s instructions ...
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bioRxiv - Genetics 2022Quote: ... cells were transfected with 100 ng plasmid DNA per well using Lipofectamine 3000 according to manufacturer’s instructions (Invitrogen, L3000008). The position of each cDNA was randomized between experiments to minimize positional effects ...
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bioRxiv - Microbiology 2022Quote: H1299 cells were grown to approximately 70% confluency and reovirus pcDNA3 plasmids were transiently transfected with lipofectamine 2000 (ThermoFisher) at a ratio of 1:2.5 (DNA:Reagent ...
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bioRxiv - Evolutionary Biology 2022Quote: ... The resulting PCR product was used as a megaprimer for whole-plasmid PCR using Phusion DNA polymerase (Thermo Fisher) (Sarkar and Sommer 1990) ...
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bioRxiv - Evolutionary Biology 2022Quote: PCR-amplification of the entire plasmid containing the gene of interest was performed with Phusion DNA polymerase (Thermo Fisher) using two complementary oligonucleotides containing a stretch of overlapping bases with the desired mutation in the middle (Table S2) ...
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bioRxiv - Immunology 2022Quote: ... medium was replaced and cells with stable integration of the plasmid were selected using zeocin for 2 weeks (0.4mg/ml, Invitrogen). Surviving cells were then sorted as single cells in 96-well plates and expanded to generate monoclonal cell lines ...
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bioRxiv - Genetics 2022Quote: ... Capped RNA encoding Cas9 was synthesised from XbaI-linearised pT3TS-nCas9n plasmid using the T3 mMessage mMachine Kit (Ambion). The Poly(A ...
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bioRxiv - Microbiology 2019Quote: 293T cells were transfected with eight pHW2000 plasmids each encoding one of the IAV segments using Lipofectamine 2000 (Invitrogen). Cells were incubated at 37°C ...
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bioRxiv - Cancer Biology 2019Quote: DNA fragments encoding the selected variants and the unmodified RBDwt were cloned into pDONR233 plasmids by Gateway cloning (Invitrogen) as described before 15 ...
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bioRxiv - Developmental Biology 2019Quote: ... 1×105 cells/well were plated in a 24-well dish and transfected at day 1 with 100 ng iOn vector with or without 20 ng of PBase-expressing plasmid (CAG∷hyPBase) using 0.7 μl of Lipofectamine 2000 reagent (Invitrogen). For triple-color labeling experiments ...
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bioRxiv - Biochemistry 2019Quote: ... with 3.75 μg of each plasmid using 27 µl of Lipofectamine LTX according to the manufacturer’s instructions (Life Technologies). The transfected cells were then incubated for 24 h in DMEM supplemented with 10% (by vol ...
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bioRxiv - Cancer Biology 2019Quote: ... Transfections for siRNA used Invitrogen’s Lipofectamine RNAiMAX and for plasmid DNA we used Lipofectamine LTX Plus (ThermoFisher, MA, USA). Cells were treated for 24 hours then refed with fresh growth medium for another 48 hours before collection for protein or growth assay in knockdown experiments ...
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bioRxiv - Evolutionary Biology 2019Quote: LRII reactions were performed by mixing 150 ng of the Entry Clone and 150 ng of expression plasmids (pDEST-DHFR F[1,2]-TRP1 or pDEST-DHFR F[3]-LEU2) according to manufacturer’s instructions (Invitrogen, USA). The reactions were incubated overnight at 25°C and inactivated with proteinase K ...
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bioRxiv - Molecular Biology 2019Quote: ... Rosa26::CreERT2 or Kdm2b-CXXCfl/fl mESCs were transiently co-transfected with 1 μg of Cas9-sgRNA plasmid and 3.5 μg of targeting construct using Lipofectamine 3000 (ThermoFisher). The day after transfection ...
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bioRxiv - Cancer Biology 2019Quote: ... and 8.0 μg transfer plasmid into HEK293T cells in a 10 cm dish with Lipofectamine 2000 reagent (Life Technologies), according to the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2019Quote: Cells (primary neurons or HEK293 cells) were transfected with 1.6 µg/mL of a pIRES2-EGFP plasmid vector (Invitrogen) harbouring the full-length cDNA coding for the human muscle 6-phosphofructo-1-kinase muscle isoform (PFK1-M)3 (accession number ...
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bioRxiv - Pharmacology and Toxicology 2019Quote: ... The cells were transiently transfected with 1 µg plasmid DNA with 2 µL of Lipofectamine 2000 (Thermo Fisher Scientific). 24-hours after transient transfection ...
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bioRxiv - Molecular Biology 2019Quote: ... was added to the plasmid sample prior to resolving it on a 0.7% agarose gel stained with SYBR gold (Invitrogen).
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bioRxiv - Molecular Biology 2020Quote: Purified plasmid DNA (2-3μg) was introduced to ARPE cells via transient transfection using Lipofectamine 3000 transfection reagent (ThermoFisher) or to H7 ESCs using Genejuice transfection reagent (MilliporeSigma ...
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bioRxiv - Cancer Biology 2020Quote: ... shRNA plasmids were co-transfected into HEK293TD cells along with packaging (Δ8.9) and envelope (VSVG) expression plasmids using the Lipofectamine 2000 reagent (Invitrogen) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2020Quote: ... was produced in ExpiCHO cells by transfection of 6 x106 cells/ml at 37 °C with 0.8 μg/ml of plasmid DNA using the ExpiCHO expression system transfection kit in ExpiCHO Expression Medium (ThermoFisher). One day later the cells were refed ...
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bioRxiv - Synthetic Biology 2020Quote: ... necator genomes (and plasmids) were extracted for whole-genome sequencing using the GeneJET Genomic DNA Purification Kit (Thermo Fisher). Samples were sent for library preparation (Nextera ...
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bioRxiv - Cancer Biology 2020Quote: Virus was generated by transfecting 3×106 HEK293T cells with plasmids using Lipofectamine 2000 (Thermo Scientific, Cat. No. 11668019) according to the manufacturer’s protocol ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... Both plasmids were digested with NotI and mRNA was generated with SP6 RNA polymerase using mMessage mMachine kit (Ambion). The mRNA was purified through phenol/isoamylalcohol/chloroform extraction ...
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bioRxiv - Genetics 2021Quote: ... U2OS and U118-derived cell lines stably expressing GFP or GFP-ADAR2 plasmids 26 were grown in standard medium supplemented with 0.5 mg ml−1 G418 (Gibco, Invitrogen). Cells expressing RNWG and RNAG were grown in in standard medium supplemented with 0.5 mg ml−1 G418 (Gibco ...
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bioRxiv - Cell Biology 2021Quote: ... PCR products (with/without stop codon) amplified from coding sequence (CDS) were first cloned in the pDONR207 plasmid (Invitrogen). The resultant entry clones were subsequently transformed into gateway binary vectors ...
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bioRxiv - Biophysics 2020Quote: ... were cloned into the plasmid pCellFree_03 for expression with N-terminal GFP tag.30 Each plasmid (60 nM) was added to Leishmania tarentolae extract31,32 supplemented with RnaseOUT (1:1000, Invitrogen) and the mixture was incubated at 28 °C for 2.5 h.
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bioRxiv - Immunology 2020Quote: ... a mix of PEImax (1 μg/μL) with expression plasmids (312.5 μg/L) in a 3:1 ratio in OptiMEM (Gibco) were added to the cells ...
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bioRxiv - Neuroscience 2021Quote: ... Indels were introduced by transfecting 8×105 cells with 1.5 μg plasmid in 100 μl scale using the Neon Transfection System (ThermoFisher) with one pulse at 1500 millivolts for 30 milliseconds (∼40-70% transfection efficiency) ...
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bioRxiv - Cell Biology 2020Quote: ... for single protein expression or (plasmids of interest – 0.2 µg + 0.2 µg, blank DNA – 0.6 µg) using lipofectamine 3000 (Thermo Fisher Scientific) or the Neon electroporator (Invitrogen ...
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bioRxiv - Molecular Biology 2021Quote: ... HEK293 and Huh-7 cells were transfected with plasmid DNAs by standard calcium phosphate precipitation and Lipofectamine 3000 (Invitrogen), respectively.
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bioRxiv - Microbiology 2020Quote: ... For each well 1.5 μl Lipofectamine 200 was mixed with 800 ng plasmid DNA in 100 μl Optimem (Thermofisher). Cells were fixed in 3% FA 18h post transfection.
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bioRxiv - Microbiology 2021Quote: ... A549 (ATCC) cells were transduced as described previously (27) with hACE2 plasmid and cultured in F-12 medium (GIBCO) with 10% FBS ...
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bioRxiv - Molecular Biology 2020Quote: ... 2 μg of plasmid was transfected into HOS cells at 40% confluency using Lipofectamine 3000 transfection reagent (Thermo Fisher) according to manufacturer’s instructions ...
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bioRxiv - Cell Biology 2020Quote: Cells in the 6-well plates were transfected with 2μg plasmid of AMPKα or OGG1 via 6 μl Lipofectamine 2000 (Invitrogen) for 6 hours following the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2020Quote: The transfection of the plasmid DNA and short interfering RNA (siRNA) was performed using polyethylenimine (PEI) and RNAiMAX (Invitrogen), respectively ...
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bioRxiv - Genomics 2020Quote: Low passage SH-SY5Y cells in the undifferentiated growth phase were transfected with the plasmids via Lipofectamine 3000 (Invitrogen) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2021Quote: ... PCR amplicons (532 bp) were purified and subsequently cloned into pCR4-TOPO plasmid by TOPO-TA cloning (Thermo Fisher) for sequencing ...
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bioRxiv - Microbiology 2021Quote: ... The standard curve for lipL32 was generated using a copy of the lipL32 amplicon cloned into pCR2.1-TOPO plasmid (Invitrogen). Normalized copy numbers were compared using an unpaired t test with two-tailed p values and 95% confidence interval (Prism v ...
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bioRxiv - Microbiology 2019Quote: The cells were seeded on cover slips overnight and were transfected with the appropriate plasmids using Lipofectamine 2000 (Invitrogen), according to the manufacturer’s protocol ...
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bioRxiv - Evolutionary Biology 2019Quote: ... or HDHD-NDA-RA-pGL4.27 along with 500 ng pRL-CMV (Renilla reporter plasmid) via electroporation (Neon Transfection System, Invitrogen). Firefly and Renilla luciferase activity were analyzed using the Dual-Glo Luciferase Assay System (Promega ...
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bioRxiv - Immunology 2021Quote: ... and co-transfected with pMD2.G and psPAX2 packaging plasmids into HEK293T cells using polyethylenimine (PEI, Fisher Scientific, #NC1014320). Virus-containing supernatants were harvested at 60-72 h post-transfection ...
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bioRxiv - Evolutionary Biology 2021Quote: ... PCR products with ∼1000 bp were cloned into plasmid vectors using TA Cloning® kit (Life Technologies, Carlsbad, California). Colonies were screened by blue/white selection ...
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bioRxiv - Genomics 2021Quote: ... and the plasmid DNA was sequenced using the BigDyeTM Terminator v3.1 Cycle Sequencing Kit (Applied Biosystems, Thermo Fisher Scientific) and primers listed in Supplementary Table S4.
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bioRxiv - Genomics 2021Quote: ... and the plasmid DNA was sequenced using the BigDyeTM Terminator v3.1 Cycle Sequencing Kit (Applied Biosystems, Thermo Fisher Scientific) and primers listed in Supplementary Table S4.
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bioRxiv - Genomics 2021Quote: ... The Myc-RARaa mRNA was synthesized from this plasmid by in vitro transcription (mMESSAGE mMACHINE sp6 transcription kit, Invitrogen) and injected into fertilized zebrafish eggs ...
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β-amyloid−driven synaptic depression requires PDZ protein interaction at AMPA-receptor subunit GluA3bioRxiv - Neuroscience 2021Quote: ... The resulting pSinRep5 plasmids were used to produce infective Sindbis pseudo viruses according to the manufacturer’s protocol (Invitrogen BV). Sindbis virus infection was achieved by injecting diluted virus into slices 20-52 hours prior to the experiments.