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Citations for Thermo Fisher :
8201 - 8250 of 10000+ citations for Human UFL1 shRNA Plasmid since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2020Quote: ... pseudokinase domain of mouse and human MLKL were expressed in Sf21 insect cells using the Bac-to-Bac system (ThermoFisher Scientific) and purified using established procedures28 ...
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bioRxiv - Cell Biology 2020Quote: Small interfering RNAs (siRNAs) specifically targeting human CD44 (siRNA IDs s2681 and s2682) were obtained from Life Technologies (Carlsbad, CA, USA). DPSCs (1×105 per well ...
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bioRxiv - Cell Biology 2019Quote: ... cells were subsequently pulsed at 370C for different time points with 25µg/ml Alexa flour-488 or- 568-conjugated human transferrin (Invitrogen, ThermoFisher Scientific) reconstituted in starvation medium and clarified by centrifugation at 15,000 × gmax at 40C for 10 minutes ...
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bioRxiv - Cancer Biology 2020Quote: ... were prepared on the Ion Chef System using the Ion Ampliseq Chef DL8 materials and the Ion AmpliSeq Transcriptome Human Gene Expression Panel Chef-ready Kit (Thermo Fisher). Libraries were then purified via solid phase reversible immobilization (SPRI ...
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bioRxiv - Microbiology 2019Quote: ... Plates were washed 6 times and incubated with horseradish peroxidase (HRP)-conjugated mouse anti-human IgG Fc secondary antibody (Cat# 05-4220; ThermoFisher Scientific) diluted 1:1000 in 100 µl blocking buffer for 1 h at room temperature or 37°C ...
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bioRxiv - Developmental Biology 2020Quote: ... Relative mRNA levels of a panel of inflammation-associated genes were initially screened using a Human Immune Taqman PCR array (4418718, ThermoFisher Scientific) and Taqman Fast Advanced Master Mix ...
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bioRxiv - Neuroscience 2020Quote: Human iPSCs were maintained as feeder-free cells in Essential 8 medium on vitronectin-coated plates (all from Gibco, Carlsbad, CA) (69) ...
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bioRxiv - Developmental Biology 2020Quote: Whole-cell protein extracts were isolated from human cells with conventional RIPA lysis buffer and protein concentration assayed by BCA kit (Thermo Scientific). Blocking was carried out in 10% skim milk in PBST for 1 h ...
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bioRxiv - Cancer Biology 2022Quote: ... RNA samples were used for global gene expression profiling on human Clariom S Assay microarrays (Thermo Fisher Scientific, Wilmington, DE, USA), which include 24351 genes ...
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bioRxiv - Immunology 2022Quote: ... Blots were washed 3x with PBS-T and incubated for 1 h at room temperature with appropriate secondary antibodies: mouse IgGk-BP-HRP (cat# sc-516102, SantaCruz) or goat anti-human-HRP (cat# 31410, Thermo Scientific), diluted 1:10,000 in 5% blocking buffer ...
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bioRxiv - Cell Biology 2022Quote: ... cells were pulsed with either 100µg/ml transferrin from human serum conjugated with Alexa Fluor™ 647(#T23366; Thermo Fisher Scientific) or vehicle (PBS ...
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bioRxiv - Neuroscience 2021Quote: ... Expression levels of Kif11 and APP were then measured by multiplex qRT-PCR using mouse Kif11 and human APP primer probes (Applied Biosystems, Mm01204225_m1 ...
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bioRxiv - Neuroscience 2021Quote: His-tagged human dynamin 1 was expressed using the Bac-to-Bac baculovirus expression system (Thermo Fisher Scientific, Waltham, MA, USA) and purified as described previously (66) ...
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bioRxiv - Neuroscience 2021Quote: ... of human iPSC-induced neurons were plated on completely dry PDL- (100 µg/ml) and laminin-(10 µg/ml; Thermo Fisher) coated 96-well or 6-well plates ...
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bioRxiv - Molecular Biology 2021Quote: ... slides were washed thrice for 5 min in PBS before incubation with Alexa 594 goat-anti rabbit secondary antibody at 1:1,000 and Alexa Fluor 488 goat anti-human IgG(H+L) at 1:500 (both Invitrogen; in PBS) for 2 h at RT ...
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bioRxiv - Bioengineering 2022Quote: The human monocyte cell line THP-1 was purchased from ATCC (TIB-202) and routinely cultured in RPMI 1640 medium (Life Technologies) containing 10% (v/v ...
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bioRxiv - Immunology 2021Quote: ... 100 units penicillin and 100 μg streptomycin per ml) supplemented with 50 U/ml IL-2 and 1 × 106 CD3/CD28-coated Human T-Activator Dynabeads/ml (Life Technologies). The next day ...
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bioRxiv - Immunology 2021Quote: ... were incubated with 2.105 primary human mast cells (cell line established from a different donor) with or without anti-CD3/CD28-coated beads (Dynabeads™, Gibco) at a T cell:bead ratio of 10:1 in RPMI 10% FCS supplemented with 20 ng.mL−1 SCF ...
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bioRxiv - Immunology 2021Quote: ... BV421 anti-human CD69 together with Live/Dear-near IR (1:1000 in PBS; LD-NIR; ThermoFisher Scientific; Cat no L10119) for exclusion of dead cells ...
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bioRxiv - Immunology 2021Quote: ... The remaining cells were enriched for CD8+ cells with Dynabeads™ Untouched™ Human CD8 T Cells Kit (Thermo Fisher #11348D) following the manufacturer’s protocol ...
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bioRxiv - Genetics 2019Quote: CNVs were detected using the Affymetrix Genome-Wide Human SNP Array 6.0 (Cat No. 901182, ThermoFisher Scientific Inc., Waltham, MA, USA) following the manufacturer’s protocol.
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bioRxiv - Neuroscience 2019Quote: ... Human IgG and IgM was quantified in blood serum (1:1000 dilution) and culture supernatants (DILUTION) using the Human IgG/IgM total ELISA Ready-SET-Go! Kits (Invitrogen/eBioscience). All ELISAs were performed as per manufacturer’s protocols ...
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Neutralizing PD-L1 and PD-L2 Enhances the Efficacy of Immune Checkpoint Inhibitors in Ovarian CancerbioRxiv - Cancer Biology 2020Quote: ... Cells were re-suspended at 100µL/well with 1:1000 diluent with anti-human IgG-Alexa 488 (#A28175, ThermoFisher, Waltham, MA). Plates were incubated for 1 hour at 4°C ...
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bioRxiv - Genomics 2019Quote: ... The digested tissue was mixed with 40 ml of CRB (1% human albumin (CSL Behring) in 40 ml of DPBS -/- (Gibco, #14190094)) and shaken vigorously to liberate the stromal cells ...
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bioRxiv - Immunology 2019Quote: ... MRC5 human fibroblast cell line and A375 human melanoma cell line are cultured at 37°C under 5% CO2 in DMEM medium (Life Technologies) containing 1% glutamine ...
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bioRxiv - Neuroscience 2019Quote: Aß1-40 and Aß1-42 concentrations from cleared supernatants of differentiated iPSCs were quantified by using the Amyloid beta 40/ 42 Human ELISA Kits (#KHB3441 and KHB3481; Thermo Fisher) according to the manufacturer’s recommendations ...
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bioRxiv - Microbiology 2019Quote: ... gondii tachyzoites were cultured in human foreskin fibroblasts (HFF) and in Dulbecco’s modified Eagle’s medium (DMEM) with GlutaMAX (Invitrogen, California, United States) supplemented with 10% fetal bovine serum and maintained at 37°C with 5% CO2.
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bioRxiv - Pathology 2020Quote: ... SMCs were transfected with control siRNA or siRNA targeting the human calpain-2 (Ambion - Silencer Select validated siRNA, Thermo Fisher Scientific) sequences using RNAiMax lipofectamine transfection reagent (Life Technologies ...
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bioRxiv - Cell Biology 2019Quote: ... hiPSC-CMs were reprogrammed from human cardiac fibroblasts as described previously (28) and maintained on Matrigel Membrane Matrix (Thermo Fisher Scientific) in mTeSR™ medium (Stem Cell Technologies ...
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bioRxiv - Systems Biology 2019Quote: ... Human ESCs (WA-01; National Institutes of Health registry #0043) were maintained in Essential 8 medium (Gibco, Grand Island, NY, USA) and 1% penicillin-streptomycin-amphotericin B solution (Lonza ...
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bioRxiv - Immunology 2019Quote: ... The isolated CD8+ T cells were washed and resuspended at a concentration of 1 × 106 cells per ml in completely recon-stituted RPMI supplemented with 50 units/ml IL-2 and 1 × 106 CD3/CD28-coated Human T-Activator Dynabeads (Life Technologies) per ml ...
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bioRxiv - Biochemistry 2019Quote: ... DSF was performed on human BACE1 (1 μg) using 96-well qPCR plates (AB Biosystems, UK) with 20x Sypro Orange (Invitrogen, UK) in 50 mM sodium acetate ...
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bioRxiv - Biochemistry 2019Quote: The human embryonic kidney (HEK) 293FT cells a high transfection efficiency isolate of the HEK293T cells were purchased from ThermoFisher (# R70007), and used without further authentication ...
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bioRxiv - Microbiology 2019Quote: ... CR-Fc and DC-SIGN-Fc and then stained with Alexa Fluor® 488 goat anti-human IgG antibody (Life Technologies) diluted 1/200 in PBS + 1% FCS or BB buffer and incubated 30 min on ice ...
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bioRxiv - Molecular Biology 2019Quote: Human atherosclerotic plaque or mouse aortic root specimens were first cut into 8 μm sections using a freezing microtome (Thermo Scientific). For immunofluorescence detection to detect Tf ...
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bioRxiv - Biochemistry 2019Quote: Protein concentration in g ⋅ L−1 of human serum was calculated using PierceTM Bicinchoninic Acid (BCA) Protein Assay Kit (Thermo Fisher Scientific ...
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bioRxiv - Genomics 2019Quote: ... were immortalized with human telomerase reverse transcriptase (hTERT) and cultured in complete AmnioMAX C-100 Basal Medium (ThermoFisher Scientific, Carlsbad, CA) supplemented with 15% AmnioMAX supplement (ThermoFisher Scientific ...
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bioRxiv - Neuroscience 2019Quote: ... and cultured in a 10 cm dish containing irradiated MEFs in human embryonic stem cell (huES) medium containing DMEM-F12 (Life Technologies), knockout 10% serum replacement (Life Technologies) ...
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bioRxiv - Cancer Biology 2019Quote: ... isolated T-cells were cultured at 2×106/mL in R10 media (RPMI 10% heat inactivated FBS) in the presence of 20 μl/ml of Dynabeads Human T-Activator CD3/CD28 (from Life Technologies). These activated primary T cells were tested as for the MJ cells assays in 3 different formats ...
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The tumour microenvironment shapes dendritic cell plasticity in a human organotypic melanoma culturebioRxiv - Immunology 2019Quote: ... A de-epidermized human dermis was obtained by incubating the dermis for one month in PBS containing gentamicin (0.5 mg/ml; Life Technologies, Inc.) and antibiotic/antimycotic (Life Technologies ...
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bioRxiv - Biophysics 2019Quote: Human embryonic kidney cells 293 (HEK293-6E, NRC, Canada) were cultured in FreeStyle F17 expression medium (Thermo Fisher Scientific, Waltham, MA) supplemented with 2 mM L-glutamine (Invitrogen ...
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bioRxiv - Biophysics 2019Quote: HeLa human cervical cancer cells transfected in conjunction with fluorescence ubiquitination cell cycle indicator (FUCCI) cell cycle sensor (Life Technologies, P36237) were kindly donated by Dr ...
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bioRxiv - Cell Biology 2019Quote: The region from −2662 to −1 bp (relative to translation start site) of the human TMEM135 promoter was synthesized by Life Technologies. Additionally ...
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bioRxiv - Microbiology 2020Quote: The spike protein gene from MERS-CoV strain Camel/Qatar/2/2014 (GenBank accession number KJ650098.1) was codon optimized for humans and synthesized by GeneArt (Thermo Fisher Scientific). The synthesized S gene was cloned into a transgene expression plasmid comprising a modified human cytomegalovirus immediate early promoter (CMV promoter ...
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bioRxiv - Genetics 2021Quote: The human lung epithelial cell line Calu-3 (UC Berkeley Cell Culture Facility) was maintained RPMI media supplemented with Glutamax (Thermo Fisher), 10% FBS (HyClone) ...
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bioRxiv - Immunology 2021Quote: ... Plates were then washed and incubated for 1h at room temperature with horseradish peroxidase (HRP)-conjugated goat anti-human IgM (Invitrogen #A18835), goat anti-human IgA (Jackson #109-036-011) ...
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bioRxiv - Genomics 2021Quote: Human embryonic kidney 293T (HEK293T) cells were cultured in DMEM media (TH.Geyer, L0102) supplemented with 10% FBS (Thermo Fisher, 10500-064) and 100 U/ml Penicillin and 100 μg/ml Streptomycin (Thermo Fisher) ...
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bioRxiv - Immunology 2021Quote: ... residues 306–577 of MERS-CoV S with a C-terminal monomeric human IgG Fc-tag and an 8x HisTag (SARS-CoV RBD-SD1) were transiently transfected into FreeStyle293F cells (Thermo Fisher) using polyethylenimine ...
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bioRxiv - Immunology 2021Quote: ... with unconjugated rabbit anti-human phosphorylated NF-κB p65 (Clone: Ser536 93H1) followed by goat anti-rabbit IgG (H+L) Alexa Fluor 488 (Thermo Fisher). For p38 ...
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bioRxiv - Immunology 2021Quote: ... and either left them untreated (hence naive) or activated them ex vivo with Dynabeads Human T-Activator CD3/CD28 (ThermoFisher Scientific), wherein the bead-to-cell ratio was 1:1 ...