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Citations for Thermo Fisher :
8001 - 8050 of 10000+ citations for Human UFL1 shRNA Plasmid since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2021Quote: ... SARS-COV-2.SctΔ19 Wuhan plasmid was amplified by PCR with Phusion DNA polymerase (Thermo Scientific, ref F-549S) and the following primers ...
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bioRxiv - Cell Biology 2019Quote: ... Transfections of transfer vector (pLentiCRISPRv2 SCRA, pLentiCRISPRv2 LAMA5) and packaging plasmids (CMV-VSVg and Delta8.9) were performed using Lipofectamine 2000 (Invitrogen) according to manufacturer’s instructions ...
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bioRxiv - Biochemistry 2021Quote: ... Plasmids (3 µg) were introduced into HEK 293 cells (1×106) by electroporation with Neon electroporator (Thermo Fisher Scientific) according to the manufacturers protocol ...
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bioRxiv - Biochemistry 2021Quote: ... 1.75 µg total plasmid DNA was mixed with 5.5 µl PEI in 150 µl Opti-MEM I Reduced Serum Media (Gibco) and incubated for 30 min at room temperature ...
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bioRxiv - Biochemistry 2021Quote: ... The two plasmids were transiently co-transfected into HEK293F cells in FreeStyle™ 293 Expression Medium (ThermoFisher Scientific, UK). The cells were cultured in suspension phase at 37 °C ...
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bioRxiv - Biochemistry 2021Quote: ... Cells were transfected 24 hr after seeding with 0.75 µg of the bi-fluorescent reporter plasmid using Lipofectamine 3000 (Invitrogen) as per manufacturer’s protocol ...
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bioRxiv - Molecular Biology 2021Quote: Plasmids expressing TSC22D4 alleles and Akt1 were transiently co-transfected to Hepa1-6 cells using Lipofectamine 2000 (Invitrogen, #11668019) according to the manufacturer’s instructions ...
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bioRxiv - Developmental Biology 2021Quote: The full-length coding region of eIF4E-5 was PCR-amplified using the plasmid 4E5-pCR2.1 as a template (Hernández et al., 2005) and subcloned into pRSET (Invitrogen, V35120) to create an expression construct ...
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bioRxiv - Immunology 2020Quote: ... VH and VL plasmids were mixed at 1:2 ratio and were transfected into Expi293F cells (Thermo Fisher Scientific), which were cultured at 37 °C and 8% CO2 for 5 days ...
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bioRxiv - Immunology 2021Quote: ... The plasmid was used for transient transfection of FreeStyle 293F cells using the FreeStyle MAX reagent (Thermo Fisher Scientific). The spike trimer was purified from filtered supernatant on Streptactin XT resin (IBA Lifesciences ...
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bioRxiv - Neuroscience 2021Quote: ... 2018) and pSTC1-Neo DNA plasmids (10:1 ratio) using Lipofectamine LTX & PLUS reagent (Cat. 15338, Thermo Fisher Scientific), with a DNA:Lipofectamine LTX:PLUS reagent ratio of 2.5:6.5:1.5 ...
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bioRxiv - Immunology 2022Quote: ... The plasmids were then transfected into MIA PaCa-2 cells using Lipofectamine 2000 reagent (Invitrogen, cat. no.11668-019). Single cell clones were sorted (GFP) ...
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bioRxiv - Genomics 2022Quote: ... cortical neurons were transfected using 1μg of peGFP-N1 plasmid along with 2 μl per well of Lipofectamine 2000 (Invitrogen) after 12 days in culture ...
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bioRxiv - Biochemistry 2021Quote: ... corresponding pET28aMBP plasmids encoding TconTS enzymes were digested with Eco105I and HindIII Fast Digest restriction enzymes (Thermo Scientific, Germany) to isolate the LD ...
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bioRxiv - Developmental Biology 2021Quote: ... Neuro-2a cells were transfected with wild-type (WT) or mutant (MUT) reporter plasmids by using Lipofectamine 3000 (Invitrogen). At 24 hours post transfection ...
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bioRxiv - Cell Biology 2020Quote: ... 2×106 NP-TTD were transfected with 3 µg plasmid via electroporation using Neon™ Transfection System (MPK1096, Invitrogen) with following parameters ...
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Splicing variation of BMP2K balances endocytosis, COPII trafficking and autophagy in erythroid cellsbioRxiv - Cell Biology 2020Quote: ... The cells were transfected after 24 h with plasmid DNA using Lipofectamine® 2000 Transfection Reagent (Thermo Fisher Scientific) according to the manufacturer’s protocol.
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REEP4 is recruited to the inner nuclear membrane by ELYS and promotes nuclear pore complex formationbioRxiv - Cell Biology 2020Quote: ... 1.8×105 cells were seeded in one well of a 24-well plate and transfected the next day with 0.4 µg plasmid DNA using Lipofectamine 2000 (Invitrogen) according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2020Quote: ... along with the pOGG4 (4 µg) plasmid were transfected into cells using 7.5 µL of Lipofectamine 3000 (ThermoFisher, L3000001). All transfections were performed in 6-well culture dishes at 80% confluency ...
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bioRxiv - Microbiology 2021Quote: ... in 6 well plates were transiently transfected with 2μg of either wild-type or mutant SARS-CoV-2 S protein plasmid with Lipofectamine 3000 (Invitrogen) at a ratio of 1:2:2 DNA ...
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bioRxiv - Microbiology 2021Quote: ... 1 μg of linearised plasmid was incubated for 2 h at 37°C with 1U T7 RNA polymerase (ThermoFisher), 5 mM DTT ...
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bioRxiv - Immunology 2020Quote: ... This plasmid was used to transiently transfect freestyle 293-F cells using the FreeStyle MAX reagent (Thermo Fisher Scientific). The spike ectodomain was purified from filtered supernatant on Streptactin XT resin (IBA Lifesciences) ...
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bioRxiv - Molecular Biology 2021Quote: ... Pen/strep were removed from the growth media ∼3 hours prior to transfection of plasmid vectors using Lifofectamine2000 (ThermoFisher) according to the manufacturer’s protocol ...
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bioRxiv - Molecular Biology 2021Quote: ... then co-transfected with 0.25 µg of pcDNA™5/FRT/TO and 2.25 µg of pOG44 Flp recombinase expression plasmid (Invitrogen) using Lipofectamine LTX with Plus Reagent (Invitrogen) ...
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bioRxiv - Microbiology 2020Quote: ... and RLuc RNAs were in vitro synthesized from these plasmid templates using the MEGAscript T7 Transcription Kit (ThermoFisher Scientifc).
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bioRxiv - Microbiology 2020Quote: ... plasmids harbouring target sequences under T7 promoter regulation were in vitro transcribed with MEGAscript RNAi Kit (Thermo Fisher Scientific), purified with ssDNA/RNA Clean and Concentrator (Zymo Research) ...
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bioRxiv - Immunology 2021Quote: ... 100mL cultures of Expi293F cells at a density of 2.5×106 cells/mL were transiently transfected with 50µg each heavy and light chain encoding plasmids and Expifectamine (Invitrogen) per manufacturer’s protocol ...
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bioRxiv - Genomics 2020Quote: ... the point mutation was generated in the RFA1 plasmid from the Molecular Barcoded Yeast ORF library from Thermo Fisher Open Biosystems ...
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bioRxiv - Microbiology 2021Quote: The wild-type and variant Ugandan and Brazilian ZIKV were produced by transfecting 293T cells with 0.5 μg of each plasmid via Lipofectamine 2000 transfection reagent (Invitrogen). Virus-containing supernatants were harvested 48 hrs post transfection ...
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bioRxiv - Microbiology 2021Quote: Cells were transfected with recombinant plasmid p3xFLAG-NS5A or NS5A mutant expression vectors using Lipo3000 (Thermo Fisher Scientific, USA) according to the manufacturer’s directions ...
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bioRxiv - Immunology 2020Quote: ... restriction enzyme was used to remove unmodified plasmids and the PCR products were transformed into Top10 (Thermo Scientific, USA) E.coli for amplification ...
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bioRxiv - Immunology 2020Quote: ... USA) were transfected with 2 plasmids: B45 encoding gp96-Ig (source: University of Miami) and pcDNA™ 3.1(-) (Invitrogen), encoding full-length SARS-CoV-2 protein S gene (Genomic Sequence ...
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bioRxiv - Developmental Biology 2021Quote: Mouse C2C12 cells (ATCC CRL-1772) were transfected with plasmid pGL4.26 BRE2 using Lipofectamine 3000 (Thermo Fisher Scientific, L300015) according to manufacturer’s instructions ...
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bioRxiv - Genomics 2020Quote: ... POT1wt and POT1V29L plasmids were transfected into HEK293T cells at 70–80% confluence using Lipofectamine 2000 (Thermo Fisher Scientific). At 48 hours post-transfection ...
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bioRxiv - Cell Biology 2021Quote: ... sgRNA/Cas9 and homology repair template plasmids were co-transfected using the Neon™ transfection system (Thermo Fisher Scientific). Transfection was performed according to the manufacturer’s guidelines (for HeLa cells ...
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bioRxiv - Cell Biology 2019Quote: ... cells were transfected at least 24 hours after seeding with 500-800 ng of plasmid using Lipofectamine 2000 (ThermoFisher), according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2021Quote: ... 1×106 cells were transfected with 1ug of PX459 gRNA2_CTCF_KO/Cas9 and 1ug of PX459 gRNA6_CTCF_KO/Cas9 plasmids using Lipofectamine 2000 (Thermo Fisher Scientific, 11668019) according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2021Quote: ... 3×105 cells were transfected with 0.5ug of PX459 CTCF_KO_gRNA3/Cas9 and 1ug of PX459 CTCF_KO_gRNA10/Cas9 plasmids using Lipofectamine 2000 (Thermo Fisher Scientific, 11668019) according to the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2020Quote: ... For overexpression of miR-7704 the MDA-MB-231 cells were grown in six-well plates and transfected for 48h with pPRIME-miR-7704-CMV-GFP-FF3 plasmid (5.5 µg/well) using Lipofectamine-2000 reagent (ThermoFisher). As control ...
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bioRxiv - Cancer Biology 2022Quote: ... cells were transfected with 5 µg of pCS2P+dkk1GFP plasmid in using Lipofectamine 2000 (Thermo Fisher Scientific, MA, USA). Dkk1GFP CM were collected on the 2nd ...
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bioRxiv - Cell Biology 2022Quote: ... 2007) and DsRed2-Mito (Tanaka Bio) plasmids using a ratio of 7:3 using Lipofectamine LTX Plus (ThermoFisher, 15338100) for 5 hours ...
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bioRxiv - Cell Biology 2022Quote: ... CRISPR-Cas9 nickases and the donor plasmid were transfected by electroporation (Neon Transfection System, Thermo Fisher Scientific, Waltham, MA) instead of a polymer-mediated transfection reagent.
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bioRxiv - Molecular Biology 2022Quote: ... HeLa RAD54KO cells were transfected with 3μg of RAD54-GFP or eGFP plasmid per well in a 6-well plate using Lipofectamine LTX PLUS (Invitrogen). Stable cell lines were generated by selecting with neomycin (800μg/ml ...
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bioRxiv - Cancer Biology 2022Quote: ... Dilute 2.5μg of lentiviral expression plasmid and 2.5 μg of Lenti-Pac HIV mix into 200μl of Opti-MEM® I (Invitrogen). In a separate tube diluting 15 μl of EndoFectin Lenti into 200μl of Opti-MEM I ...
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bioRxiv - Biochemistry 2022Quote: ... HEK293 cell lines were transfected with pOG44 and pcDNA5/FRT/TO plasmids harboring respective FLAG constructs using Lipofectamine3000 (Invitrogen) as transfection reagent according to the manufacturer’s instructions ...
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bioRxiv - Biochemistry 2022Quote: The expression plasmids of soluble spike ectodomain proteins were constructed by DNA fragments synthesized at GeneArt (Thermo Fisher Scientific) followed by cloning into the phCMV3 vector by Gibson assembly ...
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bioRxiv - Cell Biology 2022Quote: ... and these plasmids were combined with the appropriate destination vectors (see Table 4) in LR Clonase II reactions (Invitrogen) according to manufacturer instructions ...
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bioRxiv - Cell Biology 2022Quote: ... was transfected into Lenti-x 293T cells with 2:1 (psPAX2/pMD2G) plasmids using Lipofectamine 2000 (Thermo Fisher # 12566014) at a ratio of 1:1 (Lipofectamine 2000/nucleic acid (μg) ...
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bioRxiv - Cell Biology 2022Quote: ... U2OS or JMYKO-1A cells cultured in 12-well plates were transfected with 2-5µg of linearized plasmid encoding GFP or GFP-JMY derivatives using LipofectamineLTX (Invitrogen). 24h later ...
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bioRxiv - Developmental Biology 2022Quote: ... and pGL4.74-Renilla luciferase plasmids in a ratio of 1:1:0.1 (4 μg/6-well dishes) by using Lipofectamine LTX PLUSTM (Invitrogen). The luciferase activity was assayed using the Dual-Luciferase Reporter Assay Kit (Promega) ...