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Citations for Thermo Fisher :
7901 - 7950 of 9091 citations for Recombinant Human SELE Fc His tagged since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2020Quote: ... and target genes were amplified for 12 cycles of PCR using the Ion AmpliSeq Human Gene Expression Core Panel (Thermofisher). This panel contains a pool of 20,802 amplicons (41,604 primers ...
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bioRxiv - Biophysics 2020Quote: Expression was conducted according to the manual of 1-Step Human High-Yield Mini in vitro translation (IVT) kit (Product No. 88891X) distributed by ThermoFisher Scientific (Pierce Biotechnology ...
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bioRxiv - Cancer Biology 2021Quote: ... and Non – metastasis human colon cancer cell line (HT-29) were cultured in Dulbecco’s modified Eagle’s medium (DMEM: Gibco, Thermo Fisher, USA) and metastasis human colon cancer cell line (SW-620 ...
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bioRxiv - Cell Biology 2021Quote: Human ProtoArray® Microarray (v5.0) containing 9483 unique human proteins expressed as GST-fusion in insect cells were purchased from Invitrogen. Arrays were blocked in 4°C cold blocking buffer (50 mM HEPES pH 7.4 ...
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bioRxiv - Molecular Biology 2022Quote: ... samples were washed and stained for flow cytometry using appropriate seconday antibodies: goat anti-human fluorescein isothiocyanate (FITC; ThermoFisher A18812), anti-mouse Alexa Fluor 488 (Cell Signaling Technology 4408S) ...
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bioRxiv - Immunology 2022Quote: ... CD4+ T cells and NK cells were isolated from cryopreserved PBMCs using commercial kits (MagniSort Human CD4+ T Cell Enrichment; Affymetrix, and MagniSort™ Human NK cell Enrichment ...
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bioRxiv - Biochemistry 2022Quote: ... After 3hrs from release of the nocodazole block cells were double transfected with pEGFP-human LA (EGFP-wt LA and mutants) and RFP-HP1α using Lipofectamine 2000 (Invitrogen) in accordance with the manufacturer’s protocol at early passages having 60-70% confluency and were kept in culture for 24 hrs.
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bioRxiv - Cell Biology 2022Quote: The human cDNA encoding zDHHC2 was codon optimised for protein expression in insect cells and synthesized by GeneArt technology (Invitrogen). It was inserted with N and C-terminal tags (His6 and StrepII ...
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bioRxiv - Bioengineering 2022Quote: ... immortalized human dermal fibroblast cell line (Stephens et al., 2004) were cultured in Dulbecco’s modified Eagle medium (DMEM, Gibco™) supplemented with 10% fetal bovine serum (FBS ...
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bioRxiv - Molecular Biology 2022Quote: ... Paraffin-embedded human tissues and hind paws were cut into 5 μm sections with the Microtome HM355S (Thermo Fisher Scientific) and transferred onto microscope slides.
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bioRxiv - Immunology 2022Quote: For RNA extraction from human lung resection material 50∼100 mg of RNAlater-stabilized lung tissue pieces were added to 1ml of Trizol (Invitrogen) and homogenized ...
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bioRxiv - Microbiology 2022Quote: J774.1 mouse macrophages (ATCC) or primary neonatal normal human dermal fibroblasts (Neo-NHDF) (ATCC) were cultured in 10 mL DMEM (Gibco, ThermoFisher) in T75 flasks (VWR ...
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bioRxiv - Microbiology 2022Quote: To evaluate expression levels of A24Rwt and A24Rcd, human 293T cells (105 cells, 12-well plates, triplicates) were transiently transfected using Lipofectamine 2000 (LPF2000, Invitrogen) with 1 µg of pCAGGS expression plasmids encoding C-terminal HA-tagged A24Rwt or A24Rcd ...
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bioRxiv - Bioengineering 2022Quote: ... zsGreen/Oatp1b3) into human embryonic kidney (HEK 293T) cells using Lipofectamine 3000 according to the manufacturer’s lentiviral production protocol (Thermo Fisher Scientific Inc. ...
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bioRxiv - Genomics 2022Quote: Cardiac differentiation of human iPS cells was performed using a stage-based protocol in RPMI-1640 (ThermoFisher Scientific, Waltham, MA) supplemented with 0.5 mg/mL recombinant human albumin (Sigma-Aldrich ...
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bioRxiv - Cell Biology 2022Quote: ... mature human small intestinal colonic organoids grown with the expansion media were digested into single cells using TrypLE Express (ThermoFisher) and seeded into Matrigel-coated 96-well culture plate (Corning ...
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bioRxiv - Immunology 2022Quote: ... Globin mRNA was depleted from a portion of each total RNA sample using the GLOBINclear™-Human kit (Thermo Fisher). Following the removal of globin transcripts transcriptome profiles were generated via mRNA sequencing ...
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bioRxiv - Cell Biology 2022Quote: Human and mouse lung samples were inflated with a 4% w/v paraformaldehyde (PFA) solution in PBS (14190250, Thermo Fisher) then placed in 4% PFA overnight for fixation ...
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bioRxiv - Bioengineering 2022Quote: ... The xeno-free epidermal bioink was formulated with 2 x 106/ mL human KCs in 500 μL of EpiLife medium (#MEPI500CA; ThermoFisher) supplemented with HKSdaFREE and HKGE (AvantBio ...
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bioRxiv - Microbiology 2022Quote: Human monocytes/macrophages U937 and Thp1 were cultured in RPMI-Roswell Park Memorial Institute media supplemented with 10% FBS (Gibco) at 37°C in a humidified incubator (Panasonic ...
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bioRxiv - Molecular Biology 2022Quote: Wild type and MED14-dTAG human colorectal carcinoma HCT-116 cells [25] were grown in RPMI 1640 medium (Gibco, 21875034) supplemented with 10% FBS (Gibco ...
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bioRxiv - Bioengineering 2022Quote: Human iPSC-derived Brain Microvascular Endothelial-like cells were treated with 25 μg/mL fluorescent transferrin conjugate (Thermo Fisher Scientific) and incubated at 37oC for 30 min ...
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bioRxiv - Molecular Biology 2022Quote: ... Cells were washed 3 times with PBS and incubated for 1 hour with Alexa 488 nm or Alexa 594 nm conjugated secondary antibodies specific for human and rat IgG (Invitrogen) diluted 1:2,000 in 3% BSA in PBS and containing 1 μg/ml DAPI ...
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bioRxiv - Molecular Biology 2022Quote: Human embryonic kidney 293T (HEK 293T) cells were maintained in Dulbecco’s modified Eagle’s medium (DMEM; Gibco™, Cat no. #12800017) supplemented with 10% (v/v ...
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bioRxiv - Immunology 2022Quote: ... The abundance of select cytokines was then measured in 50μL of the supernatants with a Human Cytokine Magnetic 10-Plex Panel (ThermoFisher, LHC0001M) according to the instructions of the manufacturer ...
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bioRxiv - Cancer Biology 2022Quote: ... the ST1 cells were transfected with NICD pCMV Neo/intracellular domain of human Notch1 (NIC-1) plasmid using Lipofectamine 200 (Invitrogen), and then were harvested 48 hours after transfection for western blot and in vitro angiogenesis assay.
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bioRxiv - Cancer Biology 2022Quote: ... as well as NCM-356 (ATCC CVCL-D875) non-transformed human colonic mucosal cells were maintained in McCoy’s media (Thermo Scientific) supplemented with 10% FBS (Gemini Bio-Products) ...
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bioRxiv - Immunology 2020Quote: DNA encoding antibody variable domains was amplified by PCR from the pHEN1 phage display vector and cloned into a human IgG1 expression vector based on pCEP4 (Invitrogen). After validation of the cloning by Sanger sequencing ...
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bioRxiv - Neuroscience 2020Quote: The human cells SH-SY5Y were grown in D-MEM (Dulbecco’s Modified Eagle medium; Gibco®, Life technologies Paisley, UK) supplemented with 10% fetal bovine serum and 1% of penicillin/streptomycin solution (100 U/mL) ...
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bioRxiv - Neuroscience 2020Quote: The human cells SH-SY5Y were grown in D-MEM (Dulbecco’s Modified Eagle medium; Gibco®, Life technologies Paisley, UK) supplemented with 10% fetal bovine serum and 1% of penicillin/streptomycin solution (100 U/mL) ...
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bioRxiv - Neuroscience 2020Quote: RNA from human cerebral organoids was extracted according to the manufacturer’s protocol supplied with TRIzol reagent (15596018, Thermo Fisher Scientific). The concentration and purity of the RNA samples were measured using Spectrophotometer (Beckman Coulter).
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bioRxiv - Biochemistry 2021Quote: ... the cells in each well were transfected with 20 pmol siRNA targeting to human XPO1 (GenePharma, Shanghai, China) using lipofectamine 2000 (Invitrogen). At 6 h after transfection ...
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bioRxiv - Molecular Biology 2020Quote: Efficiency of all qPCR probes was tested by using a simplex 2-fold serial dilution of Quantigene commercial reference human RNA (Invitrogen) or DNA (Promega ...
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bioRxiv - Immunology 2020Quote: ... and SARS-CoV-2 and the N-terminal peptidase domain of human ACE2 (residues Ser19-Asp615) were expressed using the Bac-to-Bac baculovirus system (Invitrogen) as previously described 18,19,37-39 ...
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bioRxiv - Biochemistry 2020Quote: In vitro 3D epidermal skin equivalents were generated as previously described.[28] Human neonatal keratinocytes (HEKn, ThermoFisher Scientific, Loughborough, UK) were maintained as per manufacturer’s instructions ...
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bioRxiv - Microbiology 2020Quote: We purified polyclonal IgG from human plasma samples using Pierce™ Protein G Ultra Link™ Resin (Thermo Fisher Scientific) following the manufacturer’s protocol ...
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bioRxiv - Cell Biology 2021Quote: ... Fibrin formation was visualized by spiking whole blood with human fibrinogen conjugated to Alexa Fluor 647 (Cat. No. F35200, Invitrogen, used at final concentration of 7.5 μg/mL) ...
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bioRxiv - Cancer Biology 2021Quote: ... PLK2 in a pDONR223 vector was obtained from human ORFeome (Open Biosystems-Horizon Discovery) and was cloned into pCW57.1 using the Gateway recombination reaction (Life Technologies) and verified by sequencing prior to transfection ...
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bioRxiv - Neuroscience 2020Quote: ... and the SH-SY5Y human neuroblastoma cell line was maintained in 10% FBS Minimum Essential/F12 medium (MEM/F12, Gibco). Both cells’ media included 1% antibiotic/antimycotic mix (Gibco) ...
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bioRxiv - Microbiology 2021Quote: ADAP1CRISPR and CtrlCRISPR TM were left resting or stimulated with Dynabeads human T-activator anti-TCR/anti-CD28 (Gibco, 11132) (1 bead/cell ...
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bioRxiv - Microbiology 2021Quote: ... and the N-terminal peptidase domain of human ACE2 (residues Ser19–Asp615) were expressed using the Bac-to-Bac baculovirus system (Invitrogen) as described previously21 ...
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bioRxiv - Immunology 2020Quote: ... Acquired DDA .raw files were searched against the human UniProtKB/SwissProt database (v2012_07) using Byonic (Protein Metrics) embedded in Proteome Discoverer (ThermoFisher Scientific) to obtain peptide sequence information ...
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bioRxiv - Microbiology 2021Quote: Individual plasmids encoding 11 human genes or empty control vector were transfected individually into U-87 MG cells with Lipofectamine 3000 (Invitrogen) 48 hours prior to infection with VEEV-TC-83-nLuc.
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bioRxiv - Microbiology 2021Quote: Huh-7 cells seeded in 6 well plates were transfected with human WWOX-specific or random siRNAs (DHARMACON) at a final concentration of 50nM per well using Lipofectamine 2000 reagent (INVITROGEN). At 24 hours post siRNA transfection ...
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bioRxiv - Microbiology 2022Quote: ... Primary antibodies were detected by probing with biotin-conjugated anti-rat antibody (1:1,000) and Alexa 488-conjugated anti-human antibody (1:1,000) followed by Alexa 594-conjugated streptavidin (Invitrogen, 1:1000). Slides were then stained with 1ug/mL DAPI ...
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bioRxiv - Microbiology 2022Quote: ... gondii parasites of the type I RH strain Δku80/Δhxgprt genetic background (ATCC PRA-319) were grown in human foreskin fibroblasts (HFFs, ATCC SCRC-1041) maintained in DMEM (GIBCO) supplemented with 3% inactivated fetal calf serum (IFS ...
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bioRxiv - Genetics 2022Quote: ... Genotyping was done using Axiom™ Human Genotyping SARS-CoV-2 Research Array as instructed by the manufacturer (Thermo Scientific).
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bioRxiv - Immunology 2022Quote: A recombinant fragment of human SP-D (rfhSP-D) was expressed under bacteriophage T7 promoter in Escherichia coli BL21 (λDE3) pLysS (Invitrogen), transformed with for plasmid containing cDNA sequences for neck ...
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bioRxiv - Immunology 2022Quote: Human bronchial epithelial cells (BEAS-2B cells, ATCC® CRL-9609™) were cultured in LHC-9 medium (Life Technologies) and human lung fibroblast (MRC-5 cells ...
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bioRxiv - Immunology 2022Quote: ... were generously provided by Anita Koshy (University of Arizona) and maintained by serial passage through human foreskin fibroblast (HFF) monolayers in parasite culture medium (DMEM [Gibco] ...