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Citations for Thermo Fisher :
751 - 800 of 10000+ citations for Recombinant Human GC Protein since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2024Quote: ... 50 μL PCR reactions were prepared as follows: 10 μL GC buffer (ThermoFisher Scientific), 1 μL 10 mM dNTP mix (ThermoFisher Scientific) ...
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bioRxiv - Immunology 2019Quote: ... the wild-type and mutant (C302/305R) recombinant Mul1 proteins were produced in Sf9 cells using the Bac-to-Bac Baculovirus expression system (Invitrogen, Carlsbad, CA, USA) and purified by a nickel-chelating resin (Life Technologies ...
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bioRxiv - Cell Biology 2021Quote: In vitro binding experiments were performed with recombinant GST fusion proteins purified from E.coli and immobilized on glutathione-Sepharose 4B beads (Life Technologies #17-0756-01). Purification of GST fusion proteins was performed as described (Ebnet et al. ...
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bioRxiv - Neuroscience 2020Quote: ... The same recombinant Cdk5/p25 protein used for the MST assays was labeled with biotin using EZ-LinkNHS-PEG4-Biotinylation Kit (Thermo Scientific, Waltham, MA) and excess reagent was removed with a spin desalting column as per the manufacturer’s instruction ...
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bioRxiv - Immunology 2020Quote: The plasmid expressing the soluble pre-fusion version of SARS-CoV-2 S was used to produce the recombinant protein using the FreeStyle™ 293 Expression System (Thermo Fisher Scientific), according to the manufacturer’s instructions ...
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bioRxiv - Biochemistry 2019Quote: ... A concentration range of indicated recombinant proteins was analyzed by thermal melt assays in SEC buffer using SYPRO Orange (Molecular Probes, cat.# S6651) using a Biorad CFX96 Touch Real-Time PCR C1000 System (ramping from 4 to 100°C at 1°C per minute).
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bioRxiv - Microbiology 2020Quote: ... recombinant proteins were purified by affinity chromatography as described previously (27) and loaded on NuPAGE 4-12% BisTris gels (Invitrogen, Carlsbad, CA, USA) to confirm the correct molecular weights and assess their purity.
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bioRxiv - Biochemistry 2024Quote: ... The recombinant SARS-CoV-2 Spike S1-hFc-His tagged protein used for titrations and sorting was purchased from ThermoFisher (RP-876-79). The recombinant neuraminidase (NA ...
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bioRxiv - Cell Biology 2020Quote: ... 50ng/mL recombinant murine EGF (Gibco, PMG8043), 100ng/mL recombinant human Noggin (Peprotech ...
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bioRxiv - Cell Biology 2020Quote: ... 50ng/ml recombinant EGF (Thermo Fisher Scientific), 10% conditioned medium from a cell line producing Noggin (kind gift from Hans Clevers) ...
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bioRxiv - Immunology 2022Quote: ... The recombinant ORF8 (ThermoFisher, Cat. RP-87666) was conjugated with FITC using the FITC conjugation kit (Abcam ...
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bioRxiv - Molecular Biology 2019Quote: ... and RNaseOUT recombinant ribonuclease inhibitor (Invitrogen, 10777019) was added to lysate (1ul per 100ul lysate) ...
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bioRxiv - Genomics 2019Quote: ... 0.25 μL RNaseOUT Recombinant Ribonuclease Inhibitor (Invitrogen), was then added to each well ...
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bioRxiv - Cell Biology 2021Quote: ... Recombinant Cas9 2.0 (Thermo Fisher Scientific, A36498) was mixed with gRNA and incubated for 15 min at room temperature ...
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bioRxiv - Neuroscience 2021Quote: ... Recombinant pSinRep5 and helper plasmid pDH26S (Invitrogen) were transcribed in vitro into capped RNA using the Megascript SP6 kit (Ambion) ...
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bioRxiv - Microbiology 2022Quote: ... and 0.2 ng/mL recombinant EGF (Gibco). The transfection of primary NHKs to generate HPV18+ keratinocytes was performed as described previously [86] ...
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bioRxiv - Molecular Biology 2022Quote: ... RNaseOUTTM Recombinant Ribonuclease Inhibitor (Thermo Fisher Scientific) and Protoscript II reverse transcriptase (New England BioLabs ...
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bioRxiv - Physiology 2022Quote: ... Recombinant PIK3CG was purchased from Thermo Fisher.
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bioRxiv - Developmental Biology 2022Quote: ... Recombinant TrueCut Cas9 V2 (Thermo Fisher Scientific) was diluted in resuspension buffer R ...
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bioRxiv - Immunology 2024Quote: ... RNaseOUT™ Recombinant Ribonuclease Inhibitor (10777019, Invitrogen); Deoxynucleotide (dNTP ...
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bioRxiv - Cancer Biology 2024Quote: ... and 0.2 ng/mL recombinant EGF (Gibco). HPV18+ NHKs were generated as described previously [86] ...
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bioRxiv - Cell Biology 2024Quote: ... RNaseOUT™ Recombinant Ribonuclease Inhibitor (Thermo Fisher), Random Hexamer Primers (Thermo Fisher) ...
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bioRxiv - Immunology 2021Quote: ... Protein secretion levels in culture supernatant were quantified via ELISA for human IgG (Invitrogen, 50-112-8849) and human BAFF (R&D Systems ...
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bioRxiv - Genomics 2020Quote: ... Human PDCD1 or PDL1 cell surface protein staining was analyzed using anti-PD-1 (ThermoFisher clone MIH4) or anti-PD-L1 (ThermoFisher clone MIH1 ...
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bioRxiv - Cell Biology 2021Quote: Peak lists were searched against the complete human proteome (UniProt release 2014_08, with 68.049 proteins) using SEQUEST (Proteome Discoverer 1.3, ThermoFisher) as search engine ...
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bioRxiv - Molecular Biology 2021Quote: ... Then recombinant dsDNA fragment (add to > 400 ng/μl final concentration) or recombinant single-strand DNA (ordered from Invitrogen or IDT ...
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bioRxiv - Biochemistry 2020Quote: GC-1spg cells with inducible DAZL expression were maintained in DMEM high glucose medium (ThermoFisher) supplemented with 10% (v/v ...
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bioRxiv - Developmental Biology 2020Quote: ... GCs were collected by filtration of the remaining cells through 40μM cell strainers (Fisher Scientific).
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bioRxiv - Cancer Biology 2021Quote: ... Genotype information of Singapore GC patients was extracted from the .CEL files produced from Affymetrix Genome-Wide Human SNP 6.0 Arrays (GSE31168 ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... the samples were analyzed on TRACE 1300 GC-MS system (Thermo Fisher Scientific, MA, USA) equipped with AI1310 autosampler and Thermo TG-5MS column (length ...
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bioRxiv - Evolutionary Biology 2020Quote: ... Each C-terminally HA tagged GC-C variant was synthesized via gene synthesis (Life Technologies). GC-C was then cloned into the lentiviral transfer vector pUltra (Addgene #24129 ...
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bioRxiv - Microbiology 2021Quote: The samples above were detected using DSQ II single quadrupole GC-MS (Thermo Fisher Company). The injection port temperature of the instrument was maintained at 250 °C ...
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bioRxiv - Cell Biology 2021Quote: ... cells were transfected either with a control siRNA (Stealth Negative Control Medium GC Duplex, Invitrogen) or with siPFKFB4 (Dharmacon Smartpool siGenome D-006764-01/02/04/17 ...
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bioRxiv - Microbiology 2023Quote: ... Concentrations of the gaseous compounds were monitored automatically with a TRACE 1310 GC (Thermo Fisher Scientific ...
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bioRxiv - Genomics 2023Quote: ... 12.5 µl GC-rich Phusion High-fidelity 2× master mix (Thermo Fisher, cat. no. F532S), 1 µM primer P5 ...
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bioRxiv - Cancer Biology 2023Quote: ... Stealth RNAi Negative Control Medium GC (cat. no. 12935-300; Invitrogen; Thermo Fisher Scientific, Inc.) was used as the negative control ...
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bioRxiv - Cancer Biology 2023Quote: ... Stealth RNAi Negative Control Medium GC (cat. no. 12935-300; Invitrogen; Thermo Fisher Scientific, Inc.) was used as the negative control ...
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bioRxiv - Pathology 2024Quote: ... Transfection of GC cell lines was conducted using Lipofectamine RNAiMAX (#13778075, Invitrogen, Waltham, Massachusetts, USA) following previously described methods (25) ...
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bioRxiv - Developmental Biology 2024Quote: ... a stealth RNAi negative control medium GC duplex was purchased from Invitrogen (Carlsbad, CA, USA). Predesigned siRNAs targeting human FLNA (#2316-1 ...
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bioRxiv - Microbiology 2020Quote: ... the recombinant proteins were produced in their respective expression systems according to standard procedures and purified using either Ni-NTA (ThermoFisher Scientific, Waltham, MA, USA) or Strep-Tactin (IBA Lifesciences ...
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bioRxiv - Immunology 2021Quote: ... and 50ng purified viral RNA/DNA was incubated with the recombinant protein at different concentrations for 90min at 37°C in the presence of SUPERase In™ RNase inhibitor (Invitrogen, catalog no. AM2694), and the resulting RNA/DNA was tested for viral copies by qRT-PCR ...
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bioRxiv - Microbiology 2020Quote: ... followed by incubation with rabbit antibodies against SFTSV-N as primary antibodies and HRP-conjugated recombinant protein A/G (Cat. No. 32490, Thermo Scientific, Rockford, IL, USA) as secondary antibodies ...
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bioRxiv - Biochemistry 2023Quote: ... and the bound Arr3_ýC•IP6–Fab7 complexes incubated with HRP-conjugated Pierce recombinant protein L (cat: 32420, Thermofisher, 1:5000 dilution in ELISA buffer) for 30 min ...
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bioRxiv - Microbiology 2023Quote: ... Cells were transfected with 1 μg/ml of recombinant Tat using a commercial transfection reagent Pro-ject Protein Transfection Reagent Kit (Thermo Scientific Cat. No. 89850) following the manufacturer’s instructions ...
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bioRxiv - Biochemistry 2024Quote: ... and the bound NTCP-Fab complexes were incubated with a secondary HRP-conjugated Pierce recombinant protein L (Thermofisher, 1:5000 dilution in ELISA buffer) for 30 min ...
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bioRxiv - Microbiology 2024Quote: ... and human (goat anti-human IgG, ThermoFisher) were also used ...
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Targeted degradation of PCNA outperforms stoichiometric inhibition to result in programed cell deathbioRxiv - Cell Biology 2022Quote: Human embryonic kidney T-REx 293 cells which express the tetracycline repressor protein were purchased from Thermo Fisher Scientific and cultured in Eagle’s Minimum Essential Medium (MEM ...
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bioRxiv - Cell Biology 2022Quote: Human proteins (LIS1-SNAP, or motor domains) were expressed and purified from insect cells (ExpiSf9 cells; Life Technologies) as previously described with minor modifications3,7,20,69 ...
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bioRxiv - Biophysics 2023Quote: ... 77,897 human protein sequences) using the Sequest search engines within the Proteome Discoverer platform (version 2.4, Thermo Scientific). The following search parameters were used ...
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bioRxiv - Genetics 2021Quote: ... H3K4me1 Recombinant Polyclonal Antibody (Cat No. 710795, Invitrogen), normal rabbit and mouse IgG (Santa Cruz Biotechnology)