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Citations for Thermo Fisher :
701 - 750 of 10000+ citations for Recombinant Human GC Protein since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2021Quote: ... Solvents were purchased from the following vendors: GC-grade tributylamine 99% (ACROS ORGANICS), LC/MS grade acetic acid Optima (Fisher Chemical) ...
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bioRxiv - Genetics 2019Quote: ... GC corrected LRR of the remaining autosomal SNPs (43,316 Affymetrix, 47,512 Exome Chip). These autosomal SNPs were selected based on the following quality filters ...
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bioRxiv - Genomics 2020Quote: ... Eluted DNA was thermocycled with PfuX7 in Phusion GC Buffer (Thermo Fisher F519L) as follows ...
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bioRxiv - Microbiology 2019Quote: ... GC-MS and HPLC grade solvents were procured from Fisher Scientific (Mumbai, India). Sodium alginate (CAS No ...
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bioRxiv - Plant Biology 2019Quote: ... Samples were then injected into GC-MS (Trace 1310, ISQ LT, Thermo Scientific). The dried non-polar phase was redissolved in 800 μL of chloroform with 50 ppm BHT and 500 μL of 1.25M HCl in methanol ...
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bioRxiv - Genetics 2020Quote: ... PCR was performed using AccuPrime GC-Rich DNA Polymerase (Invitrogen, Carlsbad, CA, USA). PCR was performed following the manufacturer’s instructions with the annealing temperature of 61°C and 35 cycles ...
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bioRxiv - Microbiology 2022Quote: ... The clinical isolate was seeded on GC chocolate agar (Thermo Fisher Scientific, USA) that was supplemented with IsoVitaleX Enrichment (Becton-Dickinson ...
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bioRxiv - Microbiology 2023Quote: ... using a TRACE TR-5 GC Column (part no. 260E113P, Thermo Fisher Scientific) at 30 °C ...
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bioRxiv - Bioengineering 2023Quote: ... GC-MS (Thermo Scientific Trace 1310 coupled to a Thermo Scientific ISQ LT) and a ZBFAME column (Phenomenex ...
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bioRxiv - Synthetic Biology 2023Quote: Derivatized diterpenes were analyzed using a Trace GC Ultra gas chromatograph (Thermo Scientific) coupled to an ATAS Optic 3 injector and an ISQ single quadrupole mass spectrometer (Thermo Scientific ...
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bioRxiv - Microbiology 2023Quote: ... The anti-HSV-1 gC mouse monoclonal antibodies used were T96 (Thermo Scientific), H1413 (Virusys) ...
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bioRxiv - Cell Biology 2024Quote: ... control scrambled si RNA Low GC and Lipofectamine RNAiMAX were from Invitrogen (Thermo). siRNA smart pool of 4 oligos for BMPr2 was from Dharmacon ...
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bioRxiv - Biochemistry 2022Quote: Reactivity of recombinant S proteins from Wuhan and VOC Omicron as well as of recombinant RBD (Wuhan, Delta and Omicron) to large set (>9000) of human proteins was assessed by using protein microarrays (ProtoArray®, Invitrogen, Thermo Fischer). First microarray chips were blocked by incubation for 1 hour at 4 °C with buffer containing ...
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bioRxiv - Cell Biology 2020Quote: ... 25ng/mL recombinant murine EGF (Gibco), 50ng/mL recombinant human HGF (Peprotech) ...
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Redundant and specific roles of cohesin STAG subunits in chromatin looping and transcription controlbioRxiv - Cell Biology 2019Quote: ... and RNaseOUT Recombinant Ribonuclease Inhibitor (Invitrogen) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2019Quote: ... we added recombinant mouse IFNγ (ThermoFisher), 2-deoxyglucose (Abcam) ...
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bioRxiv - Microbiology 2022Quote: ... and RNAseOUT recombinant ribonuclease inhibitor (Invitrogen). Next ...
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bioRxiv - Cancer Biology 2020Quote: ... Recombinant IGF1 (Life Technologies, cat# PHG0071) and picropodophyllin (PPP ...
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bioRxiv - Genomics 2019Quote: ... 50ng/mL recombinant murine EGF (Gibco), and 100ug/mL recombinant human Noggin (Peprotech) ...
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bioRxiv - Cell Biology 2023Quote: ... The recombinant vitronectin (A14701SA, Thermo Fisher) was diluted in DPBS at the concentration of 1:200 to get the final concentration of 2.5 mg/ml ...
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bioRxiv - Cancer Biology 2023Quote: ... Recombinant TNFα (Thermo Fisher, cat# PHC3015) was reconstituted at 0.1mg/ml in water ...
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bioRxiv - Cell Biology 2023Quote: ... mouse recombinant Egf (Life Technologies #PMG8043), 100 ng/mL mouse recombinant Noggin (Peprotech #250-38 ...
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bioRxiv - Cancer Biology 2023Quote: ... supplemented with 5% fetal bovine serum except for RWPE-1 and RWPE-2 cell lines which were grown in a keratinocyte serum-free medium supplemented with 5 ng/mL recombinant human epidermal growth factor (EGF) and 50 μg/mL bovine pituitary extract (Gibco™, Invitrogen, Carlsbad, CA, USA). The cell lines were tested for short tandem repeat (STR ...
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bioRxiv - Cancer Biology 2024Quote: ... PWR-1E and PIN cells were cultured in Keratinocyte Serum-Free Medium supplemented with bovine pituitary extract and recombinant human epidermal growth factor (Gibco, Life Technologies, Grand Island, NY). Myc-CaP ...
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bioRxiv - Bioengineering 2022Quote: ... Anti-human IL6 Ab concentrations were quantified using the Quant-it protein assay kit (Life Technologies). Ab conjugates were validated on 10% TBE urea denaturing gels and validated on an agarose gel (Supplementary Figure S8) ...
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bioRxiv - Immunology 2022Quote: ... The secreted fusion protein was purified on a CaptureSelect™ human albumin affinity matrix (Life Technologies), and protein eluted by adding 20 mM Tris and 2.0 M MgCl2 ...
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bioRxiv - Genomics 2022Quote: ... human preadipocytes in a 6-well plate were transfected with 6.25ug Cas9 protein (Fisher Scientific, A36498) and 800ng sgRNAs (IDT) ...
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bioRxiv - Microbiology 2023Quote: Human ACE2 fusion proteins were obtained from transient transfection using the Expi293TM system (Thermo Fisher Scientific). All plasmids used in this study were generated by ATUM Bio and transient transfection was carried out following the manufacturer’s protocol for the ExpiFectamineTM 293 transfection kit (Thermo Fisher Scientific) ...
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bioRxiv - Molecular Biology 2019Quote: ... and pol μ antibodies were affinity purified from rabbit serum by coupling each full-length recombinant protein to AminoLink Coupling Resin (Thermo Scientific) and following manufacturer’s instructions for IgG purification.
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bioRxiv - Microbiology 2020Quote: Recombinant biotinylated proteins (sE, sE-cvD and DIII) were expressed at 28°C using ExpiFectamine™ 293 Transfection Kit (Thermo Fisher Scientific). Cell supernatant was harvested and dialyzed ...
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bioRxiv - Microbiology 2019Quote: ... 125ng of total recombinant protein was separated on 4-20% or 10-20% Novex WedgeWell Tris-Glycine precast gels (Thermofisher, Waltham, MA). For silver stain ...
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bioRxiv - Cell Biology 2020Quote: ... specimens were incubated with 10 nM purified recombinant POLArISact protein or 2-5 nM Alexa Fluor 488 Phalloidin (AF488-phalloidin, A12379, Thermo Fisher Scientific) and 10 mg/ml bovine serum albumin (BSA ...
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bioRxiv - Immunology 2020Quote: His-tagged recombinant mouse CD47 (as well as proteins and antibodies noted later) was labeled using AlexaFluor488 NHS Ester (Succinimidyl Ester, Thermo Fisher Scientific) reconstituted in anhydrous DMSO (dimethylsulfoxide ...
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bioRxiv - Immunology 2020Quote: ... Plates were then incubated with 1μg/ml of biotinylated recombinant ACE2 protein for 1 hour at room temperature followed by incubation with HRP-conjugated streptavidin (Thermo Fisher Scientific) for 1 hour at room temperature ...
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bioRxiv - Bioengineering 2021Quote: ... S1-RBD and ACE2 recombinant proteins were expressed in Expi293 cells for 5 days following transient transfection using Expifectamine 293 Transfection Kit (Thermo Fisher Scientific). Cell cultures were centrifuged at 4500g for 40 min ...
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bioRxiv - Molecular Biology 2022Quote: ... 0.4 picomol of folded reporter mRNA was mixed with 20 picomol of recombinant protein and 200 picomol of UltraPure BSA (Thermo Fisher # AM2618) in binding buffer (10 mM Tris-HCl ...
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bioRxiv - Molecular Biology 2022Quote: ... 28 fmol of folded reporter mRNA was mixed with 0-10 picomol of recombinant protein and 100 picomol of UltraPure BSA (Thermo Fisher # AM2618) on ice for 1 hr ...
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bioRxiv - Microbiology 2023Quote: Concentration of recombinant proteins were determined by measuring absorbance at 280 nm using a NanoDrop 1000 and/or NanoDrop One (Thermo Fisher Scientific) considering the extinction coefficient and the molecular weight calculated with the ProtParam tool (http://www.expasy.org/tools/protparam.html).
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bioRxiv - Immunology 2023Quote: ... the microtiter plates were washed 5 × with PBS-T and then incubated for 1 hour at room temperature with 2 μg/ml of biotinylated recombinant protein G (PierceTM, Thermo Fisher Scientific) for recognition of both bat and human IgG antibodies ...
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bioRxiv - Cancer Biology 2023Quote: ... Single guide RNAs (sgRNAs) were incubated together with recombinant Cas9 (TrueCut Cas9 Protein v2, 5ug/ul, Thermo Fisher Scientific, cat. no. A36499) for 15 min at room temperature ...
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bioRxiv - Genomics 2023Quote: ... Integrity of the GST tag was subsequently checked via western blotting of the purified recombinant protein using anti-GST antibodies (Invitrogen 71-7500) [1:1,500 dilution] ...
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bioRxiv - Cell Biology 2023Quote: ... and Alt-R® CRISPR-Cas9 tracrRNA Atto550 labeled, IDT) and recombinant Cas9 protein (Alt-R® S. p. HiFi Cas9 Nuclease V3, IDT) using Lipofectamine™ CRISPRMAX (Invitrogen) and cultured for 48 h in the presence of an HDR enhancer (final concentration 20 μM ...
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bioRxiv - Microbiology 2023Quote: ... AcGFP-JEVcore recombinant protein and Alexa Fluor 594 or 555-conjugated antibody (2 mg/mL, Thermo Fisher Scientific, as an injection marker) were microinjected into the cytoplasm at a final concentration of 1 mg/mL ...
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bioRxiv - Cell Biology 2024Quote: ... Expression of the recombinant LZTR1 proteins was confirmed by Western blot analysis using an anti-His tag monoclonal rabbit antibody (Thermo Fisher Scientific). Following confirmation of expression ...
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bioRxiv - Microbiology 2020Quote: Extracts were analyzed for phenanthrene content with a Trace GC Ultra system (Thermo Scientific) equipped with a 30 x 0.25 mm (0.25 µm thickness ...
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bioRxiv - Genomics 2022Quote: ... 150 nL of reverse transcription GC tagging mix (0.7 U RNAseOUT (Invitrogen, 10777-019), 1.17x first strand buffer ...
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bioRxiv - Immunology 2020Quote: ... GC-derived B cells were sorted in bulk into lysis/binding buffer (Life technologies)
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bioRxiv - Immunology 2021Quote: Decreasing number of GC B cells were cultured in 250 μl of OptiMEM (GIBCO) supplemented with 10% heat-inactivated FBS (GIBCO) ...
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bioRxiv - Genomics 2022Quote: ... DG extracts were analyzed on a Trace 1310 GC (Thermo Fisher, Waltham, MA USA) equipped with a flame-ionization detector (FID ...
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bioRxiv - Plant Biology 2024Quote: ... then redissolved in 100 μL hexane for GC-MS (ISQ-7000, Thermo Scientific, USA). The GC run sequence starts with an initial hold at 110 °C for 2 minutes ...