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Citations for Thermo Fisher :
651 - 700 of 10000+ citations for Recombinant Human GC Protein since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2022Quote: ... Recombinant Breg and GFP protein was labeled with membrane non-permeable biotin according to the manufacturer’s instructions (Thermo Fisher Scientific).
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bioRxiv - Genomics 2022Quote: ... Recombinant protein was expressed in 8L culture of Spodoptera frugiperda SF9 cells using the Bac-to-Bac system (Thermo Fisher). Cells were cultured at 27°C ...
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bioRxiv - Plant Biology 2022Quote: ... NiHa-OSR and NoFa-ICL: Fractions containing recombinant protein were pooled and dialyzed using a Slide-A-Lyzer (ThermoFisher Scientific) cassette (20 kDa MWCO ...
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bioRxiv - Plant Biology 2023Quote: ... The recombinant GFP and RPH1 proteins were enriched and purified by affinity chromatography using Ni-NTA Superflow resin (Thermo Scientific). Protein concentrations were calculated based on a standard curve created using bovine serum albumin (BSA ...
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bioRxiv - Plant Biology 2023Quote: ... Recombinant GST-tagged proteins purification were performed according to the manufacturer’s instructions of PierceTM Glutathione Superflow Agarose (Thermo Fisher Scientific), and recombinant MBP-tagged proteins purification were performed according to the manufacturer’s instructions of Amylose Resin (NEB).
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bioRxiv - Biochemistry 2023Quote: Plasmids for recombinant protein expression were constructed using mega primer insertion PCR [64] using the plasmid Champion pET303/CT-His vector (Invitrogen). The double-stranded DNA inserts (Integrated DNA Technologies ...
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bioRxiv - Molecular Biology 2023Quote: Recombinant Rpb4/Rpb7-6His protein (5 μg) was incubated in a 50 μL slurry of HisPur Cobalt Resin (Thermo Scientific) in binding buffer (20 mM Tris [pH 7.5] and 50 mM NaCl ...
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bioRxiv - Cell Biology 2019Quote: ... Log-phase GC-1spg cells were seeded in SlideFlasks (Thermo Fisher Scientific) and infected with Ad-BMP9 or Ad-GFP ...
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bioRxiv - Cell Biology 2020Quote: ... and Stealth RNAi™ siRNA Negative Control Lo GC (Ref: 12935200; Invitrogen). For plasmid transient transfection ...
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bioRxiv - Microbiology 2019Quote: ... Archaeal lipid derivatives were quantified by GC-FID (Thermo Scientific, Bremen Germany) and the carbon isotope composition was measured via GC-IRMS on a Thermo Finnigan Trace GC connected with a DELTA V Plus IRMS system via GC Isolink (Thermo Scientific ...
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bioRxiv - Cell Biology 2021Quote: ... and chloroform - were of GC/MS grade and purchased from Fisher Scientific. The cholesterol standard was purchased from Sigma-Aldrich.
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bioRxiv - Microbiology 2021Quote: Phenanthrene extracts were analyzed using a Trace GC Ultra system (Thermo Scientific) with a 30 x 0.25 mm (0.25 µm thickness ...
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bioRxiv - Neuroscience 2020Quote: ... we designed a synthetic Gdf7 gene with lower GC contents (GeneArt, ThermoFisher). Amplified genes were subcloned into pCAG ...
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bioRxiv - Biochemistry 2020Quote: ... Negative control medium GC duplex (cat no: 462001) was purchased from Invitrogen. For rhomboid knockdowns ...
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bioRxiv - Cell Biology 2020Quote: ... Stealth RNAi Negative Control Medium GC Duplex #2 (Invitrogen, Cat. #12935-112) was used.
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... Extracts were analyzed on a GC Q-Exactive Orbitrap MS (Thermo Scientific) equipped with a Thermo Trace 1300 gas chromatograph and TriPlus RSH Autosampler using chromatographic methods described previously (Elmore et al ...
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bioRxiv - Microbiology 2022Quote: ... The GC was fitted with a 30m TG-5MS column (Thermo Fisher), 0.25 mm diameter with a stationary phase of 0.25 μm ...
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bioRxiv - Genetics 2022Quote: GC-1spg cells (ATCC, CRL-2053) were cultured in DMEM (Gibco, 11965092) supplemented with 10% fetal bovine serum (Corning ...
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bioRxiv - Bioengineering 2022Quote: Sterol quantification was achieved with a Trace GC Ultra (Thermo Scientific, USA) equipped with a flame ionization detector (GC-FID ...
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bioRxiv - Cancer Biology 2022Quote: ... or recommended Stealth RNAi negative control Med GC (12935-300, Thermofisher scientific).
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bioRxiv - Developmental Biology 2023Quote: ... 1 μl solution was injected into a GC (Trace 1310, Thermo Scientific) in splitless mode equipped with a TG-5MS capillary column (30 m × 0.25 mm × 0.25 μm ...
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bioRxiv - Immunology 2023Quote: ... Analysis: Samples were analyzed with a ThermoFisher Exactive GC (ThermoFisher, Bremen, Germany) mass spectrometer Samples were introduced via Electron Impact (EI ...
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bioRxiv - Immunology 2021Quote: ... and searched against the UniProt Human protein database and RefSeq SARS-CoV-2 protein database using Sequest HT through Proteome Discoverer (Version 2.2) (Thermo Scientific, Bremen, Germany). Precursor and fragment mass tolerance were set to 10 ppm and 0.02 Da ...
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bioRxiv - Immunology 2022Quote: All experiments were performed at 4°C using ProtoArray Human Protein Microarrays (Thermo Fisher Scientific). Microarrays were blocked for 1 h in blocking solution (Thermo Fisher) ...
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bioRxiv - Microbiology 2020Quote: ... HK-2 cells were maintained in keratinocyte serum-free (KSF) media with 0.05 mg/mL bovine pituitary extract and 5 ng/mL human recombinant epidermal growth factor (Gibco - Thermo Fisher Scientific, MA, USA). All culture conditions contained a 5% antibiotic / antimycotic mixed solution (Nacalai Tesque) ...
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bioRxiv - Cancer Biology 2020Quote: ... Recombinant human IFNγ (285-IF) was purchased from R & D Systems (Minneapolis, MN) and mouse IFNγ (BMS326) from eBioscience (Thermo Fisher Scientific, Waltham, MA). The following antibodies were used for Western blot ...
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bioRxiv - Cancer Biology 2023Quote: ... supplemented with 5% fetal bovine serum except for RWPE-1 and RWPE-2 cell lines which were grown in a keratinocyte serum-free medium supplemented with 5 ng/mL recombinant human epidermal growth factor (EGF) and 50 μg/mL bovine pituitary extract (Gibco™, Invitrogen, Carlsbad, CA, USA). The cell lines were tested for short tandem repeat (STR ...
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bioRxiv - Cancer Biology 2024Quote: ... PWR-1E and PIN cells were cultured in Keratinocyte Serum-Free Medium supplemented with bovine pituitary extract and recombinant human epidermal growth factor (Gibco, Life Technologies, Grand Island, NY). Myc-CaP ...
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bioRxiv - Molecular Biology 2021Quote: ... designed using CRISPOR) and 7.5 μg recombinant Cas9 protein (Vienna Biocenter Core Facilities) using the Neon Transfection System (Thermo Fisher Scientific). A week later ...
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bioRxiv - Cancer Biology 2020Quote: ... The phage library was incubated with biotinylated CD40-Fc fusion protein or Her2 recombinant protein (Acro biosystems) for 2 hours at RT and the phage-antigen complex was captured by Dynabeads M280 (Life technologies). The bound phages were eluted by Glycin-HCl (pH 2.2 ...
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bioRxiv - Microbiology 2020Quote: One 96-well plate was coated with 200ng/well of recombinant N protein and another plate was coated with 200ng/well of recombinant 6xHis-tagged SARS-CoV-2 spike protein receptor binding domain (RBD) produced using the FreeStyle 293 Expression System (Thermo Fisher) from BEI Resources (Cat# NR-52366 ...
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bioRxiv - Biochemistry 2020Quote: ... The supernatant was added to 15 mL of glutathione agarose resin (which binds up to 40 mg of purified recombinant GST protein per milliliter of resin) (Thermo Scientific) and incubated on ice (4°C ...
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bioRxiv - Cell Biology 2021Quote: ... The His-tagged recombinant fusion protein was separated by cobalt immobilized metal chelate affinity beads using the HisPur Cobalt Purification Kit (Thermo Scientific) following the procedure described by the manufacturer ...
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bioRxiv - Microbiology 2020Quote: ... recombinant SARS-CoV-2 spike protein was conjugated with EZ-Link™ Sulfo-NHS-Biotin (1:3 molar ratio; Thermo Fisher) in Dulbecco’s PBS at room temperature for 30 min ...
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bioRxiv - Pathology 2021Quote: ... His-tag recombinant SARS-CoV-2 Spike Protein S1/S2 (S-ECD) (1 ng/ml; ThermoFisher Scientific; aa11-1208; RP-87680) or Flag-tag recombinant SARS-CoV-2 Spike RBD (1 ng/ml ...
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bioRxiv - Immunology 2021Quote: Monoclonal antibodies or recombinant SARS-CoV-2 RBD + SBD1 or NTD proteins were transfected in FreeStyle 293F suspension cells (Life Technologies) using PEIMAX transfection reagent (Polysciences) ...
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bioRxiv - Immunology 2021Quote: Thermal melt analysis of the recombinant proteins was performed in triplicate in 96-well plates in a QuantStudio 7 Flex real time PCR machine (Applied Biosystems). Each well contained 2-4 μg protein in buffer (25mM TRIS pH 8 ...
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bioRxiv - Molecular Biology 2022Quote: ... Then 3 μg of purified recombinant protein was added with 4 μl of RiboLock RNase Inhibitor (Thermo Fisher Scientific, Waltham, MA). The mixture was incubated at room temperature for 1 hour ...
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bioRxiv - Immunology 2023Quote: ... SARS-CoV-2 Spike RBD 319-541 recombinant protein (OBA0101, Ovodan Biotech) was expressed in 25 mL culture using the Expi293F expression system (#A14635; ThermoFisher Scientific) according to manufacturer’s instructions ...
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bioRxiv - Bioengineering 2023Quote: ... The cells were treated with the appropriate concentration of recombinant proteins and YO-PRO (Thermo Fisher Scientific, diluted at 1:2000) and incubated at 37°C ...
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bioRxiv - Biochemistry 2023Quote: Recombinant trimeric spike proteins (5 µg/mL) were plated in 50 µl in each well on a MaxiSorp (Thermo Fisher Scientific) microtiter plate in 1xPBS and left to incubate for at least 1 h at room temperature ...
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bioRxiv - Genetics 2023Quote: ... The presence of recombinant CENH3 proteins on the membrane was always verified by detection with the HisG epitope tag antibody (Thermo Fisher Scientific ...
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bioRxiv - Immunology 2023Quote: ... designed using CRISPOR) and 7.5 μg recombinant Cas9 protein (Vienna Biocenter Core Facilities) using the Neon Transfection System (Thermo Fisher Scientific). A week later ...
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bioRxiv - Molecular Biology 2023Quote: About 3 ml of bleed 3 was diluted in 7 ml of sterile 1X PBS and passed through 0.2 μM filter and incubated with recombinant protein G sepharose 4B beads (Life Technologies 101242) at 4℃ overnight ...
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bioRxiv - Developmental Biology 2024Quote: ... 293 cells were transfected with plasmids encoding His-tagged recombinant proteins as described in the Plasmids section using lipofectamine 2000 (Invitrogen, #11668019) following the manufacturer’s instructions (four 100 mm plates each plasmid) ...
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bioRxiv - Microbiology 2024Quote: ... Purity of the recombinant PfPKG proteins was assessed by SDS-PAGE using NuPAGETM Novex® 3-8 % Tris-Acetate gels (Invitrogen) and expression was confirmed by Western blot analysis.
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bioRxiv - Cancer Biology 2021Quote: ... The materials used for the solvents were: GC-grade Tributylamine 99% (ACROS ORGANICS), LC/MS grade acetic acid Optima (Fisher Chemical) ...
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bioRxiv - Microbiology 2021Quote: ... Solvents were purchased from the following vendors: GC-grade Tributylamine 99% (ACROS ORGANICS), LC/MS grade acetic acid Optima (Fisher Chemical) ...
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bioRxiv - Bioengineering 2020Quote: ... Acetate and propionate were measured using a GC-FID (Thermo Fisher Scientific, USA) following previously described methods (Gao et al. ...
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bioRxiv - Pathology 2021Quote: ... Solvents were purchased from the following vendors: GC-grade Tributylamine 99% (ACROS ORGANICS), LC/MS grade acetic acid Optima (Fisher Chemical) ...