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Citations for Thermo Fisher :
7851 - 7900 of 10000+ citations for EnzyFluo ERK Phosphorylation Assay Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Physiology 2023Quote: ... low glucose DMEM with heat-killed FITC-labeled Escherichia coli at 250 µg/ml (strain K-12; reagents from Vybrant phagocytosis assay kit; ThermoFisher Scientific; Cat # E2861) (ThermoFisher Scientific) ± T2R agonist for 15 min at 37°C ...
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bioRxiv - Physiology 2023Quote: ... following the manufacturer’s instructions.18 DNA extracts were quantified on a Qubit4 fluorimeter using the dsDNA HS Assay Kit (Life Technologies; USA) and were stored at −20°C before further analysis ...
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bioRxiv - Microbiology 2023Quote: ... Cell lysates were cleared by centrifugation and protein concentration was quantified using the Qubit™ Protein and Protein Broad Range Assay Kit (ThermoFisher). Protein samples were sent to the UNSW Bioanalytical Mass Spectrometry Facility for liquid chromatography-mass spectrometry (LC-MS/MS ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... Loss of plasma membrane integrity and cytotoxicity during treatments were assessed by measuring the lactate dehydrogenase (LDH) release in the culture medium using Pierce LDH Cytotoxicity Assay Kit (Thermo Scientific) according to manufacturer’s instructions.
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bioRxiv - Molecular Biology 2023Quote: ... All cells were resuspended in FACS buffer and stored on ice until flow cytometry staining of EdU+ cells using the Click-iT Plus EdU Flow cytometry assay kit according to the manufacturer’s instructions (Thermo Fisher Scienticific).
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bioRxiv - Molecular Biology 2023Quote: ... and 24 hpi (to elucidate the effect of SP-A on viral infectivity) was determined using the BCA protein assay kit (Thermo Scientific). Five micrograms of total protein were resolved by SDS-PAGE on a 10% gel under reducing conditions and transferred to PVDF membranes (Bio-Rad) ...
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bioRxiv - Immunology 2023Quote: ... and 1 × 10^6 were processed using Click-iT EdU Pacific Blue Flow Cytometry Assay Kit (Thermo Fisher Scientific Cat. #C10418). The other 1 × 10^6 were subjected to anti-CD3 crosslinking and pERK staining as described below ...
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bioRxiv - Biophysics 2023Quote: ... Protein concentrations in the supernatant were determined using the Pierce Micro BCA Protein Assay kit (Thermo Fisher Scientific, Waltham, MA, USA) according to the manufacturer’s protocol.
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bioRxiv - Immunology 2023Quote: ... A 10 μl supernatant sample was mixed with 90 μl substrate solution containing 0.5 mg/ml of 4-methylumbelliferyl N,N’-diacetyl-β-D -chitobioside in an assay buffer provided as part of the kit and in a 96-well plate (black/clear bottom plate, Thermo Fisher). The reaction was incubated at 37 °C for 30 min and then stopped by adding 100 μl stop solution ...
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bioRxiv - Microbiology 2023Quote: ... Final libraries were quantified using a Qubit 3.0 Fluorometer and associated Qubit 1X dsDNA HS Assay Kit (Thermo Fisher Scientific, USA). Library quality assessment using an Agilent TapeStation system and paired-end sequencing of the Hi-C libraries were performed at the Quadram Institute Bioscience ...
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bioRxiv - Neuroscience 2023Quote: ... Protein levels of the brain tissue were measured using the Pierce BCA Protein Assay Kit (Thermo Fisher Scientific, Waltham, MA, USA).
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bioRxiv - Microbiology 2023Quote: ... according to manufacturer’s instructions and quantified using the fluorescent dye-based Qubit® dsDNA HS Assay Kit (Invitrogen, Carlsbad, California, USA). Samples were equimolarly pooled and sequenced using the Illumina MiSeq platform (Illumina Inc. ...
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bioRxiv - Microbiology 2023Quote: ... followed by HRP-labeled anti-mouse IgG (1:50000 dilution) and quantified with Pierce™ BCA Protein Assay Kit (Life Technologies). GST-PfPP1c and PfPP1c were produced as previously described (Hollin et al. ...
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bioRxiv - Biochemistry 2022Quote: ... The supernatant was collected after centrifuging at 13,000 RPM (10 mins, 4°C) and total protein was estimated by BCA (Pierce BCA Protein Assay Kit, ThermoFisher Scientific, 23225). The supernatant was mixed with 1X Laemmli buffer and boiled at 95°C for 10 minutes ...
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bioRxiv - Cell Biology 2023Quote: ... The quantitative real-time PCR (qPCR) assays were carried out on a StepOnePlus system (Applied Biosystem) using SYBR Green Master Kit (A25918, Thermo Fisher). Primers for qPCR were listed in Table S1 ...
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bioRxiv - Cancer Biology 2023Quote: ... The concentration of the Cetuximab antibody was calculated using a Pierce™ BCA Protein Assay Kit (ThermoFisher Scientific, Waltham, MA, USA). The loading ratios and conjugation efficiencies of the two dyes were subsequently calculated using the obtained concentrations of the dyes and protein (antibody) ...
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bioRxiv - Cell Biology 2023Quote: ... Lysate protein content was normalized for each fraction using a BCA protein assay kit (Prometheus) and samples were reduced with 4x NuPAGE LDS Sample Buffer (Life Technologies) containing 10%β-Mercaptoethanol ...
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bioRxiv - Cancer Biology 2023Quote: ... and click reaction to fluorescently label incorporated EdU with AF488 following the instruction from the Click-iT Plus EdU Flow Cytometry Assay Kit (ThermoFisher; C10632).
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bioRxiv - Genomics 2022Quote: ... DNA concentration and molecule length were evaluated using the Qubit dsDNA BR Assay kit on the Qubit Fluorometer (Thermo Fisher Scientific) and the Genomic DNA Screen Tape on the Agilent 2200 TapeStation system (Agilent Technologies) ...
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bioRxiv - Genomics 2022Quote: ... and RNA quantity was confirmed using the Qubit™ RNA High Sensitivity Assay kit (Invitrogen™, Thermo Fisher Scientific, Waltham, MA) on the Qubit™ 4 Fluorometer (Invitrogen™ ...
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bioRxiv - Developmental Biology 2023Quote: ... Supernatants were collected and protein concentration was determined using a Pierce™ BCA Protein Assay Kit (Cat#: 23225, Thermo Fisher scientific) according to manufacturer’s instructions ...
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bioRxiv - Genomics 2023Quote: ... DNA quantity was determined using a Qubit dsDNA HS Assay kit in conjunction with a Qubit 2.0 Fluorometer as directed by the manufacturer (Life Technologies, USA).
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bioRxiv - Cell Biology 2023Quote: ... samples were collected by centrifugation (11,000 ×g, 20 min) and their protein content was determined (Thermo Pierce BCA Protein Assay kit, ThermoFisher, Cat# 23225). Proteins (∼30 µg/lane ...
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bioRxiv - Immunology 2023Quote: ... the Click reaction was performed using the Click-iT™ Plus OPP Alexa Fluor 647 Protein Synthesis Assay Kit (ThermoFisher, C10458) at RT for 30 mins ...
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bioRxiv - Developmental Biology 2023Quote: ... HUH7.5 cells were incubated at 37 °C for 2 hours with 10 µM EdU using the Click-iT EdU Assay Kit (Thermofisher, C10418) according to the manufacturer’s recommendations.
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bioRxiv - Developmental Biology 2023Quote: ... Protein lysate from each sample was collected and the concentration of the protein was estimated using Pierce™ BCA Protein Assay Kit (ThermoFisher). For each sample ...
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bioRxiv - Evolutionary Biology 2022Quote: ... and all negatives were checked for DNA concentration on a Qubit 2.0 Fluorometer using the Qubit dsDNA HS assay kit (ThermoFisher Scientific, USA), and for fragment size on a Fragment Analyzer using the HS NGS Fragment kit (1–6000 bp ...
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bioRxiv - Plant Biology 2023Quote: ... EMSA assays were performed according to the manufacturer’s instructions for a LightShift Chemiluminescent EMSA Kit (Thermo Fisher Scientific, Waltham, MA, USA). The migration of the biotin-labeled probes was detected using ECL substrate (Thermo Fisher Scientific ...
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bioRxiv - Genetics 2023Quote: ... The concentration of the liberated soluble protein was assessed with the Pierce BCA protein assay kit (Thermo Fisher, Waltham, MA, USA), and equal amounts of protein (180-250 µg ...
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bioRxiv - Immunology 2023Quote: ... Protein concentration of the supernatant was determined by following manufacturers instruction for the Pierce BCA Protein Assay Kit (ThermoFisher-Scientific #23227). Once total protein concentration was determined ...
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bioRxiv - Biochemistry 2023Quote: Purity of all proteins was verified by SDS-PAGE and concentration was determined using the Pierce BCA Protein Assay Kit (Thermo Scientific).
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bioRxiv - Biochemistry 2023Quote: Purity of all proteins was verified by SDS-PAGE and concentration was determined using the Pierce BCA Protein Assay Kit (Thermo Scientific).
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bioRxiv - Cancer Biology 2023Quote: ... using the Agilent Bioanalyzer to determine sample RNA integrity number (RIN) and quantified by the Qubit RNA HS assay kit (Life Technologies). All samples had RIN values > 9.7 ...
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bioRxiv - Microbiology 2023Quote: ... DNA was quantified using a Qubit 3 fluorometer in combination with dsDNA BR or HS assay kits (ThermoFisher Scientific, Frankfurt, Germany). DNA integrity was checked through spectrophotometry and DNA agarose gel electrophoresis ...
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bioRxiv - Plant Biology 2023Quote: ... The quality and quantity of the total RNA were checked using a Qubit RNA HS Assay Kit and Qubit 2.0 Fluorometer (Thermo Fisher Scientific) before sequencing ...
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bioRxiv - Microbiology 2023Quote: ... The mean concentration of DNA in each extract was estimated from three reads obtained with Qubit dsDNA BR Assay Kit (ThermoFisher Scientific).
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ALS iPSC-derived microglia and motor neurons respond to astrocyte-targeted IL-10 and CCL2 modulationbioRxiv - Neuroscience 2023Quote: Calcium flux was measured in MNs seeded in a 96 well plate using the Fluo-4 NW Calcium Assay Kit (ThermoFisher, #F36206) per the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2023Quote: The concentration of the 3C libraries was measured with the Qubit™ dsDNA HS Assay Kit (Themo Fisher Scientific™, #Q32854) using the Qubit 2.0 fluorometer (ThermoFisher Scientific™) ...
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bioRxiv - Evolutionary Biology 2023Quote: ... We used the Qubit dsDNA HS Assay Kit to quantify DNA concentrations based on the standard protocol on a Qubit 3.0 fluorometer (Thermo Fisher Scientific). Then ...
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bioRxiv - Immunology 2023Quote: ... and urine samples were stored at -80°C and analyzed to determine urinary protein concentration by a Bradford assay using the Pierce detergent compatible kit (#23246, ThermoFisher Scientific). The 24-hr urine protein amount was calculated based on 24-hr urine volume and total urinary protein concentration.
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bioRxiv - Microbiology 2023Quote: ... Final DNA concentration was quantified using Qubit dsDNA HS assay kits in a Qubit 4.0 Fluorometer (Invitrogen, Thermo Fisher Scientific, USA). Metagenomic sequencing libraries were constructed using the Nextera DNA Flex library preparation kit (Illumina Inc. ...
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bioRxiv - Neuroscience 2023Quote: ... and cDNA was quantified using a Qubit 1xdsDNA HS assay kit (cat#: Q33231) on a QuBit 4.0 fluorometer (Invitrogen, ThermoFisher Scientific). Libraries with unique indices were then pooled in equimolar ratios before sequencing.
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bioRxiv - Molecular Biology 2024Quote: ... Total protein concentration in the supernatant was determined using the Micro BCA protein assay kit (Thermo Scientific, Waltham, MA; Cat.: 23235). In the same supernatant ...
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bioRxiv - Neuroscience 2023Quote: ... Cells were lysed at 4°C with RIPA buffer and protein concentration was determined with the Micro kit BCA Protein Assay (Thermo Scientific, Courtaboeuf ...
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bioRxiv - Genomics 2024Quote: ... DNA quantity was checked with fluorometry on the DS-11 FX instrument (DeNovix) with the Qubit dsDNA HS Assay Kit (ThermoFisher Scientific) and sizes were examined on a 2100 Bioanalyzer (Agilent Technologies ...
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bioRxiv - Biochemistry 2023Quote: ... Protein concentration was measured to be 0.1 mg/ml by the BCA protein assay kit (Thermo Fisher Scientific Inc., Waltham, MA). To make cryo-specimen of Fab fragment ...
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bioRxiv - Microbiology 2023Quote: ... DNA concentration of eluates was assessed using a DS-11 FX+ Spectrophotometer (Denovix) using a Qubit™ dsDNA High Sensitivity Assay Kit (Invitrogen). 200 ng of each individually extracted sample was used to pool by cage (4 samples sent for sequencing).
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bioRxiv - Molecular Biology 2023Quote: ... The SMRTBell libraries were quality inspected and quantified on a Femto Pulse and a Qubit fluorimeter with Qubit dsDNA HS reagent Assay kit (Life Technologies). A ready-to-sequence SMRTBell Polymerase Complex was created using a Binding Kit 3.0 (PacBio ...
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bioRxiv - Microbiology 2023Quote: ... The extracted DNA was quantified using the Qubit™ dsDNA BR Assay Kit (Cat. No. Q32850, ThermoFisher Scientific, Waltham, MA, USA) and its purity was determined with a NanoDrop spectrophotometer (Table S-4) ...
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bioRxiv - Microbiology 2023Quote: ... Extracted RNA was quantified using the Qubit RNA high sensitivity (HS) Assay Kit on the Qubit Fluorometer v3.0 (Thermo Fisher Scientific) and stored at −80°C prior to library construction and sequencing ...