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Citations for Thermo Fisher :
7851 - 7900 of 10000+ citations for 7 Chloromethyl 2 2 dimethyl 2 3 dihydro 1 benzofuran since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2022Quote: ... were grown to a density of 1~2×106 cells/mL in FreeStyle 293 expression medium (ThermoFisher) supplemented with 0.1 mg/mL penicillin-streptomycin antibiotics ...
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bioRxiv - Microbiology 2022Quote: ... and plunge frozen on a Quantifoil 2/1 holey carbon grid using a Vitrobot Mark IV (ThermoFisher). A total of 18,846 movies were recorded using a Titan Krios electron microscope (ThermoFisher ...
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bioRxiv - Developmental Biology 2022Quote: ... Cells were cultured at high density and split every two days using 1-2 min TrypLE (Gibco) incubation ...
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bioRxiv - Neuroscience 2024Quote: ... and 1:600,000 External RNA Controls Consortium ExFold RNA Spike-In Mix 2 (ERCC; Invitrogen, Cat#: 4456739) in nuclease-free water (Thermo Fisher Scientific ...
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bioRxiv - Microbiology 2023Quote: ... The BA.1 and BA.2 supernatants were additionally concentrated using the Intact Virus Precipitation Reagent (Invitrogen). All viral stocks were stored at -80°C until use ...
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bioRxiv - Cell Biology 2024Quote: ... secondary antibody F(ab’)2-goat anti-mouse IgG (H+L) HRP-conjugate (1:5,000 dilution, Invitrogen), and secondary antibody goat anti-rabbit IgG (H+L)-HRP-conjugate (1:10,000 dilution ...
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bioRxiv - Developmental Biology 2024Quote: ... was prepared and added to 426.5 μL 2:1 (vol:vol) 0.1x Tris (Fisher Scientific, cat.# BP152-5)-ethylenediaminetetraacetic acid (EDTA ...
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bioRxiv - Neuroscience 2023Quote: ... primary antibodies for mouse anti-MAP2 (microtubule-associated protein 2; Invitrogen, Catalog No. 13-1500, 1:1000) and guinea pig anti-synapsin1/2 (Synaptic Systems ...
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bioRxiv - Biochemistry 2023Quote: ... and 2) adding 25 μL of N-methyl-N-trimethylsilyltriAuoroacetamide (TMS) with 1% trimethylchlorosilane (Thermo Fisher Scientific) and incubated for 30 minutes at 60°C ...
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bioRxiv - Physiology 2023Quote: ... After 1 hour incubation with 4µM Rhod-2-AM and 100 nM MitoTracker Green (Thermo Fisher Scientific) at 37°C ...
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bioRxiv - Synthetic Biology 2023Quote: ... were coated with biotin-conjugated mouse anti-HA monoclonal antibody (10 μg ml−1; 2-2.2.14, ThermoFisher) in ELISA buffer (25 mM Tris-HCl ...
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bioRxiv - Neuroscience 2023Quote: ... Equilibrated cells were then transfected using DNA/PEI particles at a 1:2 ratio in OptiMEM (Gibco). For con-transfection we used a ratio of 1:3:3:3 of receptor:Gα:Gβ:Gγ using TRUPATH plasmids (18 ...
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bioRxiv - Cell Biology 2024Quote: ... 1 kPa polyacrylamide gels were made using 2 μl of blue-fluorescent beads (200 nm; Thermo Fisher), 18.8 μl of 40% acrylamide solution (cat no ...
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bioRxiv - Microbiology 2022Quote: ... The remainder was stained using FITC-conjugated F(ab’)2-Goat anti-human IgA (Invitrogen; 1/1000) in PBS + 0.1% BSA (Fraction V ...
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Rac1, Rac3 GTPases and TPC2 are required for axonal outgrowth and migration of cortical interneuronsbioRxiv - Neuroscience 2022Quote: ... (2% B27 supplement, 0.5% glucose, 1X Glutamax, 1% Pen/Strep, in Neurobasal medium –phenol red, all Gibco) in a 5% CO2 humidified incubator at 37 °C as previously described (Vidaki et al. ...
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bioRxiv - Biochemistry 2022Quote: ... 1% nonessential amino acids (1140050) and 50μM 2-mercaptoethanol (31350-010) (all Thermo Fisher Scientific unless specified), 1,000 U/ml LIF (ESGRO ESG1107 ...
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bioRxiv - Cancer Biology 2022Quote: ... 1×106 Fh1 fl/fl or 2×106 Fh1 -/- cells were plated onto 15-cm dishes (Nunc) and allowed to reach 80% confluency ...
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bioRxiv - Developmental Biology 2022Quote: ... the cells were resuspended in 1 ml DMEM containing 2 μl Hoechst 33342 (62249, Thermo Fisher Scientific), 2 μl Zombie Aqua dye (423101 ...
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bioRxiv - Cell Biology 2022Quote: ... and aliquots of 1 × 106 cells were placed into 2-mL cryovials (Thermo Fisher Scientific, Waltham, USA). Cells were pelleted by centrifugation for 5 min at 160 x g ...
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bioRxiv - Neuroscience 2022Quote: ... washed and incubated for 2 h with Alexa Fluor 594-conjugated secondary antibody (1:1,000; Life Technologies). After a final washing step ...
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bioRxiv - Microbiology 2022Quote: ... 2 μL 20U/μl SuperaseIn and 1 μL 200 U/μL SuperScript III Reverse Transcriptase (Life Technologies). First-strand cDNA was synthesized by incubating the sample for 10 minutes at 25°C ...
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bioRxiv - Microbiology 2022Quote: ... 2 μL 20U/μl SuperaseIn and 1 μL 200 U/μL SuperScript III Reverse Transcriptase (Life Technologies). First-strand cDNA was synthesized by incubating the sample for 10 minutes at 25°C ...
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bioRxiv - Physiology 2023Quote: ... pH 7.5) and incubated for 2 hours with secondary antibodies (1:1000, Thermo fisher Scientific, MA, USA) conjugated with fluorescent dyes ...
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bioRxiv - Neuroscience 2022Quote: ... Sections were counterstained with 4’,6-diamino-2-phenylindole dihydrochloride (DAPI, 1:10000 in PBS, Thermo Scientific) and mounted with a cover glass using Fluoromount (Diagnostic BioSystems ...
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bioRxiv - Cell Biology 2022Quote: ... and incubated with AlexaFluor488-conjugated secondary antibody diluted in 2 % BSA (1:500, Invitrogen, Thermo Fisher Scientific) against the primary antibody for 40 minutes at RT ...
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bioRxiv - Cell Biology 2022Quote: ... and incubated with AlexaFluor488-conjugated secondary antibody diluted in 2 % BSA (1:500, Invitrogen, Thermo Fisher Scientific) against the primary antibody for 40 minutes at RT ...
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bioRxiv - Microbiology 2023Quote: ... stained with 1:1000 Alexa Fluor 488 labeled goat anti-rabbit IgG 2° Ab (Invitrogen, A-11070) with blocking buffer and incubated for 1 h at RT before washing again 3X with PBS.
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bioRxiv - Bioengineering 2024Quote: ... to a glass slide and sticking 1-2 stacked circular cryolabels (Fisher Scientific, Cat# 03-391-102) in the center of the spacer ...
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bioRxiv - Biophysics 2024Quote: ... and concentrated to 1-2 mg/mL using a PierceTM concentrator (3K molecular weight cutoff; ThermoFisher Scientific). 50 μg of protein was loaded in each well of a 4-20% precast polyacrylamide gel (BioRad) ...
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bioRxiv - Physiology 2024Quote: ... Fluorescently conjugated secondary antibodies were applied in 1:2 primary/secondary molar ratios (Life Technologies, Carlsbad, CA). Labeled samples were incubated in EasyIndex (LifeCanvas Technologies ...
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bioRxiv - Neuroscience 2024Quote: ... the sections were incubated for 2 hours with Alexa Fluor 649 anti-rabbit (1:500, Invitrogen, USA). Afterward ...
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bioRxiv - Synthetic Biology 2024Quote: ... F(ab’)2 goat anti-mouse secondary antibody (Alexa Fluor 647; Thermo Fisher, A-21237, 1:500) was added in 0.1% saponin in PBS and incubated with the cells for 20 min at 4°C ...
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bioRxiv - Developmental Biology 2024Quote: ... or 32°C in E3 medium supplemented with 0.2 mM 1-phenyl-2-thiourea (10107703, Acros Organics) from 8 hpf to prevent pigmentation ...
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bioRxiv - Neuroscience 2024Quote: ... Approximately 2 mg of protein sample was digested by adding trypsin (Thermo Scientific, 50:1 w/w) and incubated at 650 rpm for 6–18 h at 37°C ...
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bioRxiv - Microbiology 2024Quote: ... cells were overlaid with 0.8% agarose in DMEM supplemented with 2% NBCS and 1% antibiotic-antimycotic (Gibco). Cells were incubated at 37 °C for 48 h (MAYV) ...
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bioRxiv - Microbiology 2024Quote: ... cleared using BABB (benzyl alcohol (#1.09626.1000, Supelco, USA): benzyl benzoate (#10654752, Thermo Fisher Scientific, USA (1:2)) and imaged as follows ...
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bioRxiv - Neuroscience 2024Quote: ... Cultures were maintained in NGM (Neurobasal-A Medium, 2% B-27, and 1% Glutamax, from Gibco, ThermoFisher Scientific ...
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bioRxiv - Neuroscience 2024Quote: ... 2 M urea in 50 mM Tris-HCl (pH 7.5) with 1 mM dithiothreitol (DTT, Thermo Fisher) and 5 µg/ml trypsin (Thermo Fisher ...
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bioRxiv - Neuroscience 2024Quote: ... and the plasmid piggyBacTransposase (Systems Bioscience) in a ratio of 8:2:1 using lipofectamine 3000 (Invitrogen). Two days after transfection ...
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bioRxiv - Neuroscience 2024Quote: ... Nuclei were stained for 5 minutes with 1 µM 4’,6-diamidino-2-phenylindole dihydrochloride (DAPI; Invitrogen), coverslips were mounted on glass slides with mounting medium (Dako) ...
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bioRxiv - Biochemistry 2024Quote: ... was grown to a density of 2 × 106 cells ml−1 in Expi293 expression media (Gibco #A1435101) at 37 °C ...
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bioRxiv - Biochemistry 2024Quote: ... DNA of PP2Ac and B55 (2:1 ratio) were diluted in Opti-MEM Reduced Serum Medium (ThermoFisher). Similarly ...
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bioRxiv - Bioengineering 2024Quote: ... 2 µL mL-1 egg lecithin (5 mM in EtOH) and 1x chemical defined lipid concentrate (Gibco). After incubation for 2 days ...
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bioRxiv - Biochemistry 2024Quote: ... the right lungs were immersed in 2 mL RPMI medium with 1 mg/mL collagenase II (Gibco Life Technologies ...
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bioRxiv - Biophysics 2024Quote: ... 4-(2-hydroxyethyl)-1-piparazineethanesulphonic acid (HEPES) and tris(hydroxymethyl)aminomethane (Tris) were purchased from Fisher Scientific.
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bioRxiv - Cell Biology 2024Quote: ... The Maxima SYBR Green qPCR master mix kit [1:2] including ROX [0.18µM] (Thermo Fisher Scientific, K0252) was mixed with forward and reverse primers [268 nM] and cDNA [12.5 ng] in a final volume of 14 µL ...
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bioRxiv - Developmental Biology 2024Quote: ... batches of 4 embryos were digested in 40µl of a solution of 2:1 0.25% Trypsin (Gibco): Accutase (StemCell Technologies ...
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bioRxiv - Immunology 2023Quote: ROS was detected by mixing Chloromethyl-H2DCFDA dye (1µM; Invitrogen, #C6827) with bone marrow harvested from mice 21 days post-irradiation ...
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bioRxiv - Cancer Biology 2020Quote: Apoptosis was measured by caspase-3/7 staining using CellEvent® Caspase-3/7 Green ReadyProbes® Reagent (ThermoFisher Scientific Inc., cat# R37111). CHLA20 and NGP cells were grown until confluence on 6-well plates with six days of treatment with DMSO or GSK591 ...
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bioRxiv - Cancer Biology 2024Quote: ... 48hrs after transfection the cells were fixed and stained for activated Caspase-3/7 using Apoptosis CellEvent™ Caspase-3/7 Green Detection Reagent (Thermo Fisher Scientific) according to manufacturers’ instructions ...