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Citations for Thermo Fisher :
7651 - 7700 of 10000+ citations for 7 Chloromethyl 2 2 dimethyl 2 3 dihydro 1 benzofuran since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2024Quote: ... Slides were subsequently incubated for 2 hours in secondary antibodies (all 1:500; Thermo Fisher Scientific) diluted in FBS ...
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bioRxiv - Neuroscience 2024Quote: ... for 1 L cultures of HEK293F cells (∼2-3x106 cells/mL) in Freestyle 293 media (Gibco) supplemented with 2% FBS (Gibco) ...
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bioRxiv - Microbiology 2024Quote: ... 1 μg of peptides was captured on a 2 cm C18 trap column (Thermo Fisher 164946). Subsequently separation was executed using a 70 min gradient from 8% (v/v ...
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bioRxiv - Pathology 2024Quote: ... for 1-2 hours was followed by chemiluminescent detection by Pierce ECL (32132; Thermo Fisher Scientific) with a ChemiDoc Imaging System (Bio-Rad) ...
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bioRxiv - Microbiology 2024Quote: ... Cells were incubated with 1:1000 dilution of astrovirus type 2 monoclonal antibody (8E7) (Invitrogen, USA) for 1h at RT or overnight at 4°C ...
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bioRxiv - Molecular Biology 2024Quote: ... 1% nonessential amino acids (11140050) and 0.1 mM 2-mercaptoethanol (31350-010) (all Thermo Fisher Scientific), 12 ng/mL LIF (104278 ...
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bioRxiv - Molecular Biology 2024Quote: ... Total DNA (1 µg) was pre-treated with 2 U of Klenow Fragment (Thermo Fisher Scientific) for 15 min at 37 °C ...
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bioRxiv - Cell Biology 2024Quote: The following primary antibodies were used: rat anti-mouse alpha-tubulin (YL1/2, Invitrogen, 1:500), mouse anti-mouse acetylated-tubulin (6-11B-1 ...
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bioRxiv - Genomics 2024Quote: ... and lacking sodium pyruvate and HEPES (4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid) (McCoy‘s 5A, Gibco 16600082), supplemented with 10% fetal bovine serum (Gibco ...
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bioRxiv - Biochemistry 2024Quote: ... were incubated in warm PBS-containing 2 μM JC-1 or 40 μM DHE (Thermo Fisher) for 15 minutes in the dark ...
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bioRxiv - Neuroscience 2024Quote: ... were supplied with 20% (v/v) 2-mercaptoethanol and 1×NuPAGE LDS sample buffer (NP0007; Invitrogen), heated for 7 min at +90 °C ...
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bioRxiv - Bioengineering 2024Quote: ... The gel pieces were digested with 2 ng μL-1 of trypsin (Thermo Fisher Scientific, R001100) in 25 mM NH4HCO3 overnight at 37°C ...
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bioRxiv - Developmental Biology 2024Quote: ... 1 nL of a 2 mg/mL solution of Alexa Fluor 647-conjugated ovalbumin (Invitrogen, O34784) was injected into the extracellular space of embryos at 4 hpf.
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bioRxiv - Molecular Biology 2022Quote: A concentration of 6 × 103 cells was loaded with 5 mM 4-amino-5-methylamino-2’,7’- difluorofluorescein diacetate (DAF-FM, Molecular Probes, Thermo Fisher, Sao Paulo, Brazil) after 72 h of treatment ...
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bioRxiv - Synthetic Biology 2021Quote: ... Isolated B cells were activated at 1 x 106 cells mL-1 for 2 days in IMDM medium (ThermoFisher) with 10% fetal bovine serum ...
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bioRxiv - Genomics 2020Quote: ... 0.5-1×106 proliferating TILs were seeded with 100×106 normal donor peripheral blood mononuclear cells irradiated with 5,000 rad in a 1:1 mixture of TIL media and AIM-2 media (ThermoFisher), supplemented with 30 ng/mL OKT3 (Miltenyi Biotec ...
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bioRxiv - Genomics 2021Quote: ... After 2 days of transduction hNGN2-eGFP media was replaced with 1:1 ratio of Neurobasal A (ThermoFisher, 10888022) and DMEM/F12 medium containing GlutaMAX ...
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bioRxiv - Biochemistry 2020Quote: ... air-drying overnight and incubating with 2 μM TOTO-1 iodide (1 mM solution in DMSO, Thermo Fisher Scientific) for 15 min.
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bioRxiv - Microbiology 2021Quote: ... Previously pelleted spheroplasts were resuspended in 1 mL 10mM 4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid (HEPES) buffer solution (Gibco) and 100 μl were collected as whole spheroplasts ...
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bioRxiv - Microbiology 2020Quote: ... The monolayers were then overlaid with 1 to 2 ml of a mixture of 1 part 0.6% agarose (Invitrogen) and 1 part complete Eagle Minimal Essential Medium (VWR ...
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bioRxiv - Microbiology 2021Quote: ... 800 µl of isolation medium (DMEM, supplemented with 2% FBS, 1% penicillin-streptomycin and 1% amphotericin B, ThermoFisher Scientific) was added to each well ...
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bioRxiv - Physiology 2020Quote: ... the medium was switched to DMEM/F12 (1:1) plus 2% New Zealand fetal bovine serum (Thermo Fisher Scientific), hormones ...
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bioRxiv - Immunology 2021Quote: ... at 1:0.5:2 molar ratio using Lipofectamine™ 2000 (0.6 ul per 1 ug DNA; Invitrogen™, 11668019); growth medium was refreshed 16 h after transfection ...
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kinesin recruitment by adapter SKIP on melanosomes is dynamically controlled by LC3B phosphorylationbioRxiv - Cell Biology 2021Quote: ... The tumor was excised and was washed in homogenization buffer (0.25 M sucrose [Merck, CAS 57–50–1], 10 mM HEPES, 1 mM EDTA, 2% antibiotic and antimycotic [ThermoFisher Scientific ...
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bioRxiv - Cell Biology 2021Quote: ... for 1 h at room temperature and stained for 10 min with 4,6-diamidino-2-phenylindole (DAPI; 1 mg/ml stock solution diluted 1:50,000 in PBS; Thermo Fisher) at room temperature with at least four PBS washes in-between ...
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bioRxiv - Immunology 2022Quote: ... resuspended in 1 mL nuclei wash buffer (2% BSA and 0.2LULul−1 RNase inhibitor (Thermo Fisher, cat. no. AM2694) in 1X PBS ...
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bioRxiv - Neuroscience 2023Quote: ... p21) and pAAV target plasmid in a 4:1:1:2 molar ratio by use of Lipofectamine 2000 (ThermoFisher) according to company protocol ...
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bioRxiv - Biochemistry 2024Quote: ... and p976 were packaged in HEK-293T cells at a ratio of 2:1:1 by Lipofectamine 2000 (Invitrogen). The ratio of liposome to DNA was 2:1 ...
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bioRxiv - Neuroscience 2024Quote: ... pCMVR8.74 and pLVX-EF1α-mitoSRAI-IRES-Puro at a 1:1:2 molar mass ratio using Lipofectamine 3000 (Invitrogen). The next day ...
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bioRxiv - Molecular Biology 2023Quote: ... Inoculated cells were incubated for 1 h at 37°C and then overlaid with a 1:1 mixture of 1.2% Oxoid agar and 2× DMEM (Gibco) with 10% (vol/vol ...
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bioRxiv - Developmental Biology 2023Quote: ... washed 1 x 30 min at room temperature in PBSTE-1% BSA supplemented with 2% phalloidin-AF488 (Invitrogen A12379), washed 4 x 30 min at room temperature in PBSTE-1% BSA and washed 2×30’ at room temperature in PBSTE ...
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bioRxiv - Immunology 2022Quote: ... OT-I T-cells (∼400,000 in 1 mL growth media) were loaded with 1 μM Fura-2 AM (F1221, ThermoFisher) for 40 min RT ...
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bioRxiv - Molecular Biology 2023Quote: ... for 1-2 h at room temperature (1:1000) for viral titration or an anti-GFP antibody (A11122, Invitrogen) for measurement of oxGFP-positive cells ...
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bioRxiv - Developmental Biology 2023Quote: ... Cells were passaged with 80-90% confluence at a split ratio of 1:2-1:4 using TrypLE (Gibco) for 8 min at 37°C ...
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bioRxiv - Microbiology 2021Quote: ... The recommended quantity of purified nucleic acids were amplified using real time PCR kits for PCV-2 (LSI VetMAX™ Porcine Circovirus Type 2 Quantification, Thermo Fisher Scientific, MA, USA), Porcine reproductive and respiratory syndrome virus [PRRSV] European and North American strains (LSI VetMAX™ PRRSV EU/NA Real-Time PCR Kit ...
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bioRxiv - Genomics 2020Quote: ... and RNA integrity was estimated by separating 2 μL of each sample on a 2% agarose gel and quantified using a Nanodrop (Thermo Fisher Science, Waltham, MA, USA) and stored at −80⁰C ...
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bioRxiv - Cancer Biology 2020Quote: ... Cells were treated for with varying concentrations of BCNU (0 to 2 mM) for an additional 48 h before incubation with Calcein AM dye (2 μM) (Invitrogen; Cat# C1430, Grand Island, NY) at 25°C for 30 m ...
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bioRxiv - Genomics 2021Quote: ... RNA Polymerase II 8WG16 (Covance #MM2-126R, 2 µg/ChIP) and Phospho-RNA pol II CTD (Ser5) (Thermo Fisher Scientific #MA1-46093, 2 µg/ChIP). ChIP assays for qPCR quantification were performed using primers shown in Table 2 ...
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bioRxiv - Neuroscience 2020Quote: ... qPCR was performed for 2 genes of interest and 2 internal controls (Suppl Figure S1) using Taqman® gene expression assays (Thermo Fisher, Foster City CA) and Taqman® Fast Universal Master Mix (ThermoFisher ...
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bioRxiv - Immunology 2020Quote: ... Cells were starved in FCS-free DMEM for 2 hours prior to experiments and labeled during the last 20 minutes of starvation with 2 µM CellTracker™ Red CMTPX and 2 µg/ml Hoechst 33342 (both from Molecular Probes, Eugene, OR, USA). A gap was made in the cell monolayers of every well using an HTS Scratcher (Peira ...
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bioRxiv - Microbiology 2020Quote: ... and RNA integrity was determined by separating 2 μL of each sample on a 2% agarose gel and quantified using a Nanodrop (Thermo Fisher Science, Waltham, MA, USA) and stored at -80°C until further use ...
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bioRxiv - Immunology 2020Quote: ... were calculated using RQ-Min = 2-(ΔΔCt + T *SD(ΔCt)) and RQ-Max = 2-(ΔΔCt - T *SD(ΔCt)) from triplicate wells as suggested by Applied Biosystems (manufacturer of qPCR machines). Results shown are representative of multiple independent experiments with similar results.
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bioRxiv - Neuroscience 2022Quote: ... both proximal and distal chambers were washed with HibE imaging medium (Hibernate E [BrainBits] supplemented with 2% B-27, 2 mM GlutaMAX, 100 U/ml penicillin and 100 µg/ml streptomycin [Thermo Fisher Scientific/Gibco; 15140–122]) twice while maintaining fluidic isolation ...
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bioRxiv - Neuroscience 2024Quote: ... To precipitate the RNA, 250 μL of 2-propanol (AppliChem, Darmstadt, Germany) and 2 μL of GlycoBlue Coprecipitant (15 mg/mL) (Thermo Fisher Scientific, Waltham, MA, USA) were added to each sample ...
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bioRxiv - Microbiology 2024Quote: ... for 2 min before separation on an Easy-Spray™ C18 nano column (75 µm x 150 mm, 2 µm, Thermo Fisher Scientific, Waltham, MA) with a 107 min gradient at a flowrate of 0.3 µL/min ...
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bioRxiv - Cell Biology 2020Quote: Total cellular mRNA was isolated from cells grown in 2-D and 3-D culture conditions using the GeneJET RNA purification kit (Thermo Fisher Scientific). For analysis of germ layer markers ...
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bioRxiv - Cell Biology 2020Quote: An aliquot of each sample equivalent to 3 μg was loaded onto a trap column (Acclaim PepMap 100 pre-column, 75 μm × 2 cm, C18, 3 μm, 100 Å, Thermo Scientific) connected to an analytical column (Acclaim PepMap RSLC column C18 2 μm ...
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bioRxiv - Biochemistry 2022Quote: ... The trap column used for the nano HPLC was a 2 cm × 75 μm capillary column packed with 3 μm C18-silica particles (Thermo Fisher Scientific) and the separation column was a 12.5 cm × 75 μm capillary column packed with 3 μm C18-silica particles (Nikkyo Technos Co. ...
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bioRxiv - Molecular Biology 2021Quote: ... Peptide mixtures were loaded on a C18 Acclaim PepMap100 trap-column (75 μm ID x 2 cm, 3 μm, 100Å, Thermo Fisher Scientific) for 3.5 minutes at 5 μL/min with 2% Acetonitrile MS grade (Sigma Aldrich ...
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bioRxiv - Cell Biology 2022Quote: ... Cells were washed 3 times in PBST and mounted in 4′,6-diamidino-2-phenylindole (DAPI)-containing ProLong Anti-fade Diamond mountant (Thermo Fisher Scientific).