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Citations for Thermo Fisher :
7751 - 7800 of 10000+ citations for 7 Chloromethyl 2 2 dimethyl 2 3 dihydro 1 benzofuran since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2023Quote: ... the sample was incubated for 3 s and blotted for 2 s using the freezing plunger Vitrobot I (Thermo Fisher Scientific, USA). Grids were quickly frozen in liquid ethane cooled by liquid nitrogen and stored in liquid nitrogen until checked ...
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bioRxiv - Microbiology 2024Quote: ... Samples were loaded onto a trapping column (Acclaim PepMap 0.075 mm, 2 cm, C18, 3 μm, 100 A; Thermo Fisher Scientific Corp.) in line with a nano-LC column (Acclaim PepMap RSLC 0.050 mm ...
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bioRxiv - Plant Biology 2024Quote: ... Peptides were loaded on a trapping column (2 cm x 75 µm ID. PepMap C18 3 µm particles, 100 Å pore size, Dionex, Thermo Scientific) and separated either on a 25 cm EASY-Spray column (25 cm x 75 µm ID ...
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bioRxiv - Neuroscience 2024Quote: ... Adult brains were post-fixed for up to 24 hours with 4% paraformaldehyde and cryosectioned after 2-3 days of incubation in 30% sucrose (Thermo Fisher Scientific) in PBS ...
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bioRxiv - Molecular Biology 2024Quote: ... mCherry-LacI-FokI-WT and mCherry-LacI-FokI-D450A expressing vectors 24 were transfected in U2OS 2-6-3 cells using Lipofectamine LTX (Invitrogen; #15338-100) for 24 hours ...
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bioRxiv - Microbiology 2024Quote: ... Samples were injected and concentrated on a trap column (PepMap100 C18, 3 µm, 100 Å, 75 µm i.d. x 2 cm, Thermo Fisher Scientific) equilibrated with 0.05% TFA in water ...
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bioRxiv - Immunology 2024Quote: ... 1% L-Glutamine and plated at a density of 2-3 × 106 cells per well in a round U-bottom Nunclon Delta treated 96-well plates with lids (Thermo Fisher Scientific). Plate was then centrifuged at 500 x g for 5 min at 4 °C before being stimulated with 100 μl of complete media + 1X eBioscience Cell Stimulation Cocktail (containing phorbol 12-myristate 13-acetate (PMA) ...
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bioRxiv - Molecular Biology 2024Quote: ... MEFs and HeLa) or 2 µL of Lipofectamine 2000 (Calu-3 and VeroE6) according to the manufacturer’s recommendation (ThermoFisher and Invitrogen, respectively). For co-transfections ...
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bioRxiv - Synthetic Biology 2024Quote: ... Libraries were grown in 10 mL LB for 2-3 h and plasmids extracted using the GeneJET Plasmid Miniprep Kit (Thermo Fisher Scientific). From each library ...
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bioRxiv - Molecular Biology 2024Quote: ... peptides were concentrated using a PepMap100 C18 pre-column (75 µm x 2 cm, with 3 µm particle size; Thermo Fisher Scientific) and subsequently resolved on an EASY-Spray column (ES903 ...
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bioRxiv - Genetics 2024Quote: TBB-2-associated siRNAs were isolated from mex5p::3xHA::tagRFP::TBB-2;GFP::NRDE-3(*NLS) embryo lysates and incubated with anti-HA magnetic beads (Thermo Scientific #88836). The beads were washed extensively and eluted with HA peptide (Thermo Scientific #26184).
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... RT-qPCR was performed using a Quantstudio 3 Flex real-time PCR machine with 2× TaqMan Universal Master Mix II (Applied Biosystems, #2704844). Commercial primers/probe sets specific for mouse Gpr4 (Mm01322176_s1) ...
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bioRxiv - Neuroscience 2024Quote: ... Retinas were incubated at 4°C for 3 days in PBS containing Alexa Fluor 488–conjugated isolectin GS-IB4 (Griffonia simplicifolia, 2 μg/ml, Invitrogen, Rockford, IL) and anti-laminin primary antibody (LAMA2 ...
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bioRxiv - Cancer Biology 2024Quote: ... Sample digests were acidified with formic acid to a pH of 2-3 prior to desalting using C18 solid phase extraction plates (SOLA, Thermo Fisher Scientific). Desalted peptides were dried in a vacuum-centrifuged and reconstituted in 0.1% formic acid for LC-MS analysis.
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bioRxiv - Biochemistry 2024Quote: ... Immunoprecipitated proteins were eluted three times, each time with 100 µL of elution buffer 2 (Pierce IgG Elution Buffer, pH ∼3 (Thermo Fisher Scientific); 1% (w/v ...
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bioRxiv - Biochemistry 2024Quote: ... Sample digests were acidified with formic acid to a pH of 2-3 before desalting using C18 solid phase extraction plates (SOLA, Thermo Fisher Scientific). Desalted peptides were dried in a vacuum-centrifuged and reconstituted in 0.1% formic acid for liquid chromatography-mass spectrometry analysis.
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bioRxiv - Biochemistry 2024Quote: ... Sample digests were acidified with formic acid to a pH of 2-3 before desalting using C18 solid phase extraction plates (SOLA, Thermo Fisher Scientific). Desalted peptides were dried in a vacuum-centrifuged and reconstituted in 0.1% formic acid for liquid chromatography-mass spectrometry analysis.
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bioRxiv - Biochemistry 2024Quote: ... The clarified lysate (supernatant) was collected and mixed with 2-3 mL 50% slurry of Ni-NTA resin beads (ThermoFisher Scientific, #88221) washed twice in LB-MNA ...
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bioRxiv - Biochemistry 2024Quote: ... Sample digests were acidified with formic acid to a pH of 2-3 before desalting using C18 solid phase extraction plates (SOLA, Thermo Fisher Scientific). Desalted peptides were dried in a vacuum-centrifuged and reconstituted in 0.1% formic acid for liquid chromatography-mass spectrometry analysis.
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bioRxiv - Biochemistry 2024Quote: ... Samples (1µl) were loaded onto a C18 Acclaim PepMap100 trap-column (75 µm ID x 2 cm, 3 µm, 100Å, Thermo Fisher Scientific) for 3.5 minutes at 6 µL/min with 2% ACN ...
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bioRxiv - Cell Biology 2021Quote: ... followed by an anti-mouse secondary antibody (V5: 1 hr, RT, 1/500; Invitrogen, Cat #A21200; FLAG: 2 hr, RT, 1:200; Invitrogen, #A11004) according to the manufacturer instructions ...
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bioRxiv - Microbiology 2023Quote: ... Resuspended bacteria were then lysed (2 × 108 total CFU) by a 1:1 addition of 1% dodecyltrimethylammonium bromide (DTAB) (AC409310250; Fisher Scientific) for 5 min with agitation ...
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bioRxiv - Microbiology 2024Quote: ... 2% B27, 1% N2, 1% MEM non-essential amino acids, 1% penicillin/streptomycin and 0.1% β-mercaptoethanol, all from ThermoFisher Scientific) supplemented with dual SMAD inhibitors (10 µM SB-431542 and 100 nM LDN-193189) ...
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bioRxiv - Immunology 2024Quote: ... were coated with 1 µg/mL (full dose, or diluted to 1/2, 1/4, 1/8) of anti-CD3 antibody (16-0032-81, Thermo Fisher) in PBS at 4 °C overnight ...
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bioRxiv - Immunology 2021Quote: ... PBMCs were incubated for 20 min at 37°C in PBS containing 4,4-difluoro-5,7-dimethyl-4-bora-3a,4a-diaza-s-indacene-3-hexadecanoic acid (BODIPY FL C16; 1 μM; Thermo Fisher Scientific). To determine neutral lipid content ...
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bioRxiv - Cell Biology 2023Quote: CellEvent™ Caspase-3/7 Green Detection Reagent (Invitrogen) (1:500 ...
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bioRxiv - Cell Biology 2022Quote: CellEvent® Caspase 3/7 Green (Thermo Fisher, UK) was used to evaluate cell apoptosis ...
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bioRxiv - Cancer Biology 2023Quote: ... the CellEvent Caspase-3/7 Green Detection kit (Invitrogen) was used with propidium iodide as a viability marker ...
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bioRxiv - Cell Biology 2024Quote: ... The caspase 3/7 substrate activity assay (Thermo Fisher Scientific ...
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bioRxiv - Bioengineering 2024Quote: ... CellEventTM Caspase-3/7 green detection reagent (Invitrogen, C10423) by 1:400 to achieve 5 μM in complete growth medium ...
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bioRxiv - Cancer Biology 2024Quote: ... CellEvent Caspase-3/7 Green Detection Reagent (Invitrogen, #C10423) was added too and Caspase 3/7 positive events ...
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bioRxiv - Developmental Biology 2024Quote: ... and CellEvent caspase 3/7 detection reagent (Thermo Fisher), and incubate for 30 minutes ...
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bioRxiv - Bioengineering 2023Quote: Passage 3 hMSCs from 4 donors chosen randomly (2 male; B, G, and 2 female; C, D) were thawed and cultured in T175 flasks (Thermo Fisher Scientific, Hampton, New Hampshire USA) supplemented with RoosterNourishTM-MSC expansion medium (RoosterBio ...
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bioRxiv - Microbiology 2024Quote: Luria-Bertani (LB) broth and agar (tryptone 10 g/L, yeast extract 5 g/L, sodium chloride 10 g/L, Fisher Scientific BP1426-2 and BP1425-2) were routinely used for growth of E ...
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bioRxiv - Cell Biology 2020Quote: ... Secondary antibodies used were F(ab’)2 goat anti-rabbit Alexa Fluor 568 (Molecular Probes, 1:1000), goat anti-mouse Alexa Fluor 488 (Molecular Probes 1:1000).
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bioRxiv - Immunology 2021Quote: ... T-cells were stimulated with Dynabeads Human T-Activator CD3/CD28 (ratio 1:2, Thermo Fisher Scientific) in the presence of PBMC-derived CECs (ratio 1:2 ...
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bioRxiv - Neuroscience 2021Quote: ... the sections were incubated for 2 hours in Alexa 647-conjugated donkey anti-rabbit (1:2000; ThermoFisher), 3 times washed in PBS and mounted by Vectashield (Vector Laboratories ...
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bioRxiv - Cancer Biology 2020Quote: ... in a slide box humidified using wet paper towels (Fisher Scientific Cat # 03-448-1 or -2) for 30 minutes at room temperature ...
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bioRxiv - Cell Biology 2020Quote: ... a 1:1000 dilution of DAPI (4’,6-diamidino-2-phenylindole, dihydrochloride, Molecular Probes; D1306, Molecular Probes)/PBS solution or 5 mg/ml Hoescht (Invitrogen)/PBS solution was carried out for 15 min at RT ...
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bioRxiv - Cell Biology 2020Quote: ... a 1:1000 dilution of DAPI (4’,6-diamidino-2-phenylindole, dihydrochloride, Molecular Probes; D1306, Molecular Probes)/PBS solution or 5 mg/ml Hoescht (Invitrogen)/PBS solution was carried out for 15 min at RT ...
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Bidirectional neuronal migration coordinates retinal morphogenesis by preventing spatial competitionbioRxiv - Developmental Biology 2021Quote: ... Organoids were incubated for 2 days with primary antibodies (Anti-GFP at 1:100, A11120, Thermo Fisher Scientific ...
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bioRxiv - Neuroscience 2022Quote: ... Secondary antibodies used were goat anti-guinea pig Alexa 488 (VGlut1/2, Invitrogen A-11073, 1:200) and goat anti-rabbit Alex 594 (PSD-95 ...
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bioRxiv - Cell Biology 2022Quote: ... 1 kPa polyacrylamide gels were made using 2 μL of blue fluorescent beads (200 nm; Thermo Fisher), 18.8 μL of 40% acrylamide solution (cat no ...
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bioRxiv - Neuroscience 2021Quote: ... centrifuged and suspended in MACS buffer containing 2 mM EDTA and 1% FBS in PBS 1x (Invitrogen). Microglia were isolated with anti-CD11b conjugated magnetic microbeads (130-049-601 ...
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bioRxiv - Molecular Biology 2021Quote: The purified RNA was initially dephosphorylated in 1 x FastAP buffer containing 2 U FastAP (Thermo Scientific) and 20 U RiboLock (Invitrogen) ...
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bioRxiv - Molecular Biology 2021Quote: ... 200 µL of 2X loading buffer (1:2 diluted 4X lithium dodecyl sulfate sample buffer [Thermo Fisher NP0007] ...
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bioRxiv - Cell Biology 2022Quote: ... A 1 ml aliquot was treated with 2 μM tetramethylrhodamine methyl ester perchlorate (TMRE) (Molecular Probes, USA) and incubated at 37°C for 30 min ...
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bioRxiv - Biochemistry 2020Quote: ... and 2 mM DTT were incubated at a 1:100 molar ratio with either BS3 (Thermo Fisher) or BS2G (Thermo Fisher ...
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Therapy-induced lipid uptake and remodeling underpin ferroptosis hypersensitivity in prostate cancerbioRxiv - Cancer Biology 2020Quote: ... and Nuclear DNA was counterstained with 1 µg/ml 4’,6-diamidino-2-phenylindole (DAPI, Thermo Fisher). Alternatively ...
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bioRxiv - Bioengineering 2020Quote: The graphene-FET was immersed in 2-methoxyethanol solution containing 1 mM PBASE (Thermo Fisher Scientific Inc.) for 1 hour ...