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Citations for Thermo Fisher :
7601 - 7650 of 10000+ citations for 5 NITROFURFURYL ALCOHOL since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2022Quote: ... 293T/ACE2 cells were cultured at 37 °C with 5% CO2 in Dulbecco’s modified Eagle’s medium (DMEM, WAKO) containing 10% fetal bovine serum (Gibco) and penicillin/streptomycin (100 U/ml ...
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bioRxiv - Cell Biology 2022Quote: ... 750,000 cells per well in a 6-well plate were transfected using Lipofectamine 2000 (5 μL; Thermo Fisher) as needed ...
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bioRxiv - Cancer Biology 2022Quote: ... for 5 minutes followed by a wash with chilled PBS and blocked with 3% goat serum (Thermo Scientific) for 1h at room temperature ...
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bioRxiv - Cancer Biology 2022Quote: Transformed MEF (5. 105 cells) were resuspended in 100 μl of RPMI medium (Thermo Fisher Scientific; Waltham, MA) and injected subcutaneously into the dorsal area of 4-6 week-old immuno-deficient athymic female mice (Charles River ...
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bioRxiv - Microbiology 2022Quote: ... the media was replaced with selection media containing 1 µg/mL puromycin or 5 µg/mL blasticidin (ThermoFisher) and cells were selected for 48 h before proceeding with experiments.
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bioRxiv - Biochemistry 2022Quote: Cells were cultured at 37°C in 5% CO2 atmosphere in Dulbecco’s modified Eagle medium (DMEM, Life Technologies) supplemented with 10% heat-inactivated fetal bovine serum (FBS ...
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bioRxiv - Biochemistry 2022Quote: ... Streptavidin solution (5 µg/mL) was plated in 50µL in each well on a MaxiSorp (Thermo Fisher Scientific) microtiter plate in 50 mM sodium bicarbonate pH 8.75 ...
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bioRxiv - Neuroscience 2021Quote: ... washed once in TN buffer for 5 min and mounted with Fluoromount-GTM mounting medium with DAPI (Invitrogen).
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bioRxiv - Neuroscience 2021Quote: ... and plasmidic DNA (0.9µg/µL) in a 1:5 ratio were separately pre-incubated with Opti-MEM (Gibco). Lipofectamine 2000 and plasmidic DNA were left to complex for 20 minutes at room temperature and added to the plated BV2 cells in low glucose DMEM supplemented with 5% fetal bovine serum (FBS ...
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bioRxiv - Cell Biology 2020Quote: ... 3-5 μl of purified γ-TuRC was visualized on an SDS-PAGE with SYPRO Ruby stain (ThermoFisher) following the manufacturer’s protocol ...
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bioRxiv - Molecular Biology 2020Quote: ... low ROX (KAPA) was used for qPCR and data were acquired on a QuantStudio 5 (Thermo Fisher Scientific) and analyzed in Prism v8 (GraphPad).
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bioRxiv - Molecular Biology 2020Quote: ... 5% CO2 for 24 h before transient transfection with plasmids encoding Affimer-His constructs using Lipofectamine 2000 (ThermoFisher), as per the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2021Quote: ... or empty vector (pcDNA 5) together with scrambled or Rbfox2 specific siRNAs using Neon Nucleofection System (Thermofisher Scientific) as described previously (Verma et al ...
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bioRxiv - Cell Biology 2022Quote: ... 3T3L1 cells and Lenti-X 293T cells were cultured at 37 °C with 5% CO2 in Dulbecco’s modified Eagle’s medium (DMEM, Invitrogen) containing 10% fetal bovine serum (HyClone ...
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bioRxiv - Cell Biology 2022Quote: ... INS-1 cells were transfected in 6-well plates with 5 nM siRNA using Lipofectamine RNAiMAX (Invitrogen, 13778030) and typically analyzed after 96 hours ...
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bioRxiv - Cell Biology 2022Quote: ... seeded on t25 flasks at 37°C 5% CO2 in DMEM/F12 (1:1) (ThermoFisher Scientific, Cramlington, UK) with 10%FBS ...
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bioRxiv - Cell Biology 2022Quote: ... Cells were maintained at 37°C under 5% CO2 in the following culture medium: DMEM (Dulbecco’s modified Eagle’s medium) Glutamax (InVitroGen) containing 10% of heat inactivated fetal bovine serum ...
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bioRxiv - Immunology 2022Quote: ... supplemented with 5% heat-inactivated fetal bovine serum (FBS) and 1X Penicillin-Streptomycin solution (Thermo Fisher Scientific, USA). SARS-CoV-2 clinical isolates (D614G variant ...
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ArchR: An integrative and scalable software package for single-cell chromatin accessibility analysisbioRxiv - Genomics 2020Quote: ... MCF10A cells were ordered from ATCC and cultured in DMEM/F12 containing 5% horse serum (Thermo Fisher 16050130), 0.02 ug/ml human EGF (PeproTech AF-100-15) ...
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bioRxiv - Genomics 2019Quote: ... and supplemented with 5% fetal bovine serum (FBS) and 250 μg/ml Geneticin G418 (ThermoFisher Scientific, MA, USA). For differentiation ...
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bioRxiv - Genomics 2020Quote: ... Real-time quantitative polymerase chain reaction (PCR) was performed on QuantStudio 5 Real-Time PCR Instrument (Applied Biosystems). All experiments were done in triplicate with SYBR-GreenER pPCR SuperMix (Life Technologies) ...
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bioRxiv - Immunology 2021Quote: ... we measured the proliferation capacity of NK92 cells by an EdU (5- ethynyl-2’-deoxyuridine; Invitrogen, California, USA) proliferation assay ...
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bioRxiv - Microbiology 2020Quote: ... Cells were stained with DAPI at 5 μg/ml and mounted with Slow-Fade mounting medium (Molecular Probes) or Prolong Diamond (Thermo Fisher Scientific).
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bioRxiv - Neuroscience 2019Quote: Individual 5 dpf larvae were placed into each well of a 24-well tissue culture plate (Fisher Scientific) and transferred into a Zebrabox imaging chamber (Viewpoint) ...
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bioRxiv - Immunology 2019Quote: ... A maximum of 5×103 live CD3+CD4+ T cells was sorted into 100 μL of RNAlater (Ambion) using a FACSAria III (BD Biosciences) ...
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bioRxiv - Biochemistry 2019Quote: ... Reduction of disulfide bonds was performed with 5 mM Tris(2-carboxyethyl)phosphine (TCEP) Bond-breaker (Thermo Scientific) at RT for 1 h ...
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bioRxiv - Immunology 2019Quote: ... counted and plated at a density of 5×105 cells/well (24 well-plates) in RPMI 1640 (Gibco) 10% FBS (Dutcher) ...
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bioRxiv - Neuroscience 2019Quote: ... cDNA was synthesized by reverse transcription from 5 μg RNA using First Strand cDNA Syntesis Kit (Thermo Scientific). RTqPCR was performed on a 7500 Fast System (Applied Biosystems ...
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bioRxiv - Physiology 2020Quote: Weaned mice were ear-notched and samples were incubated in lysis buffer (100 mM Tris HCl pH 8.5, 5 mM EDTA, 200 mM NaCl, 0.2% SDS, 0.14 mg/mL Proteinase K, Ambion™) for 2 hours at 55°C under agitation (700 rpm) ...
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bioRxiv - Microbiology 2019Quote: ... RNA (5 ng µl−1) was reverse transcribed using the High Capacity cDNA Reverse Transcription Kit (Applied Biosystems). 1 µl of cDNA was used as a template in a 10 µl qRT-PCR reaction performed with Power SYBR reagent (Applied Biosystems) ...
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bioRxiv - Cell Biology 2019Quote: ... One million cells were suspended in 5 milliliters of tenogenic growth media comprising high glucose DMEM (Thermo Scientific), 10% Cellect™ Silver fetal bovine serum (MP Biomedicals) ...
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bioRxiv - Physiology 2020Quote: ... Larvae were sampled and placed in a liquid scintillation vial with 5 ml of EcoScint cocktail (Fisher Scientific). Samples were counted in a Beckman (Brea ...
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bioRxiv - Molecular Biology 2020Quote: Modified 5’ RLM RACE was performed according to the manufacturer’s instructions (FirstChoice™ RLM-RACE kit, ThermoFisher Scientific). In order to detect premature miRNAs ...
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bioRxiv - Biochemistry 2020Quote: ... and an Acclaim PepMap 100 trap column (100 μm × 2 cm, nanoViper, C18, 5 μm, 100Å; Thermo Scientific) were used to separate tryptic peptides by increasing concentrations of 80% acetonitrile (can ...
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bioRxiv - Immunology 2019Quote: ... and infected intravaginally with 5×105 FFU of PRVABC59 ZIKV in 15 µL of HBSS (Gibco, Thermo Fisher). Mock-infected mice received 15 µL of HBSS intravaginally ...
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bioRxiv - Immunology 2019Quote: ... and infected intravaginally with 5×105 FFU of PRVABC59 ZIKV in 15 µL of HBSS (Gibco, Thermo Fisher). Mock-infected mice received 15 µL of HBSS intravaginally ...
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bioRxiv - Biophysics 2019Quote: ... Nick translation was initiated by the addition of 5 µl of 250 µM biotin-11-dUTP (ThermoFisher Scientific) and 1.5 U of E.coli DNA polymerase (New England Biolabs ...
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bioRxiv - Molecular Biology 2019Quote: ... YSM tissues from day 5 embryos were treated with collagenase (collagenase type 4, 17104019, ThermoFisher, Waltham, MA, USA) to partially digest the extracellular matrix and facilitate cell isolation [17] ...
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bioRxiv - Cell Biology 2019Quote: ... The membrane was blocked with 5% BSA (GenDEPOT, A0100) in 1% TBST (Fisher Scientific, BP2471-1, BP337-100) overnight ...
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bioRxiv - Microbiology 2019Quote: ... DNA was removed from 5 µg of total RNA using the TURBO DNase kit (Invitrogen, Carlsbad, CA, USA).
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bioRxiv - Microbiology 2019Quote: One milliliter of monomeric capsid (5 mg/ml) was dialyzed in SnakeSkin dialysis tubing 10K MWCO (Thermo Scientific) using a buffer that is high in salt and contains a reducing agent (buffer 1 ...
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bioRxiv - Immunology 2019Quote: ... were then infected with EV71 viral isolates at MOI 5 in serum-free Iscove’s Modified Dulbecco’s Medium (IMDM; Gibco) (50) ...
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bioRxiv - Microbiology 2019Quote: Ligation reactions were performed with a 1:5 ratio of vector:insert using T4 DNA Ligase (15224017, ThermoFisher Scientific) in a 20ul reaction as per manufacturer’s protocol ...
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bioRxiv - Cell Biology 2019Quote: ... The cells were allowed to attach overnight prior to transfection of plasmid DNAs (0.1-0.2 μg/well) using Lipofectamine 2000 (2-5 μL/well; Invitrogen) within a small volume of Opti-MEM (200 μL ...
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bioRxiv - Immunology 2019Quote: Bacterial cultures were incubated O/N in a 5 μM solution of Draq5 Fluorescent Probe Solution (ThermoFisher Scientific). Bacterial suspensions were washed 3 times with PBS before RAW264.7 macrophages were infected.
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bioRxiv - Cancer Biology 2019Quote: ... The cell suspension was filtered through a 70 μm cell strainer and centrifuged at 1200 rpm for 5 min and the pellet was re-suspended in 0.9 M sucrose in 0.5 x HBSS (Gibco). After further centrifugation for 20 min at 2000 rpm ...
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bioRxiv - Plant Biology 2019Quote: ... Total RNA samples from approximately 5 x 108 cells were extracted using mirVana™ miRNA Isolation Kit (Ambion). RNA concentration was measured by measuring absorbance at 260nm using the Eppendorf Biospectrometer ...
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bioRxiv - Bioengineering 2019Quote: ... HS-27a and HS-5 cell lines were cultured in RPMI 1640 (Life Technologies, Villebon-sur-Yvette, France) and hMSCs and MS-5 in MEM Alpha (Life Technologies) ...
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bioRxiv - Genomics 2019Quote: ... and washed in Buffer F (50 mM Tris-HCl pH 8.0, 40% glycerol, 5 mM MgCl2, 0.5 mM DTT, 4 u/mL RNase inhibitor [SUPERaseIN, Ambion]). Washed permeabilized cells were finally resuspended in Buffer F at a density of 1×106 cells/30 μL and immediately frozen in liquid nitrogen ...
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bioRxiv - Cell Biology 2019Quote: ... 293-T-REx™ cells were cultured in the presence of 5 µg/mL blasticidin (Gibco; #A11139-03) to maintain the pcDNA™6/TR plasmid carrying the ORF for tetracycline (Tet ...