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Citations for Thermo Fisher :
7601 - 7650 of 10000+ citations for Recombinant Human FCGRT & B2M Protein His Avi Strep II tagged Biotinylated since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2021Quote: ... the following streptavidin-tagged fluorochromes were added at optimized ratios to the pMonomer solution: SA-PE (Invitrogen; Cat no S866; Lot no 2129894); SA-APC (Invitrogen ...
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bioRxiv - Immunology 2019Quote: ... was co-transfected with pHR encoding either SNAP-tagged full length CD80 (SNAP-CD80) or CD86 (SNAP-CD86) into HEK293T cells using polyethylenimine (Fisher Scientific, catalog NC1014320). 72-hour post transfection ...
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bioRxiv - Neuroscience 2020Quote: ... the Cav2.3-expressing HEK293 cells were transiently transfected with plasmids for the expression of Myc-tagged KCTD8 (AY615967) or KCTD12b (AL831725) using Lipofectamine 2000 (Invitrogen, Carlsbad, CA, USA) as described earlier (Schwenk et al. ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... Cells were then exposed to Alexa-Fluor 488 or 568-tagged secondary goat anti-rabbit Ig (1:200, Molecular Probes, Eugene, OR, USA) for 30 min at RT ...
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bioRxiv - Molecular Biology 2020Quote: ... All of the UMI-tagged template molecules were added to the reaction containing 2 U Platinum SuperFi DNA Polymerase High Fidelity (Thermo Fisher Scientific, USA), and a final concentration of 1X SuperFi buffer ...
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bioRxiv - Neuroscience 2021Quote: ... Secreted dimeric C-terminally Fc-tagged MDGA1 stably expressed in HEK293T cells was collected in serum-free Opti-MEM (Thermo Fisher Scientific, Inc.). Fc-tagged MDGA1 protein was run on an affinity column packed with Protein-G Plus Agarose fast flow resin (Pierce ...
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bioRxiv - Cell Biology 2021Quote: ... Alexa-Fluor-488- or Alexa-Fluor-568-tagged donkey anti-mouse- or anti-rabbit-immunoglobulin antibodies (A32766, A32790, A10042, A10037, Molecular Probes, 1:200) and Alexa-Fluor-633 goat anti mouse (A-21050 ...
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bioRxiv - Physiology 2022Quote: ... HPAECs were transfected with either control plasmids (empty vector) or 1μg of HA-ubiquitin plasmids (HA-tagged Ubi) using Lipofectamine 3000 (Thermo fisher Scientific, PA, USA) according to the manufacturer’s recommendations ...
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bioRxiv - Neuroscience 2023Quote: ... Cells were washed three times with 1X PBS and incubated with suitable fluorophore tagged secondary antibodies (1: 2000, Invitrogen, San Diego, CA, USA). Cells were washed with 1X PBS before mounting ...
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bioRxiv - Microbiology 2024Quote: ... Detection of the murine-human chimera 72A1 detection was performed using goat anti-mouse IgG (H+L) cross-adsorbed secondary antibody Alexa Fluor™ 488 tagged (Invitrogen #A-11001).
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bioRxiv - Molecular Biology 2024Quote: ... 2008) N-terminally tagged with the V5 epitope was synthesized as a DNA string (GeneArt Strings DNA Fragments, Thermo Fisher Scientific, Schwerte, Germany) and cloned into pcDNA3.1 to obtain pcDNA3.1-V5-RHEB-Q64L ...
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bioRxiv - Cancer Biology 2023Quote: ... Tissues were further incubated for 2 h at room temperature with HRP-tagged secondary antibody of goat anti-mouse (1:1000) (Life Technologies, Thermo Scientific, USA). The sections were then washed with TBST three times ...
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bioRxiv - Neuroscience 2023Quote: ... Slides were washed twice with phosphate-buffered saline for 5 minutes and incubated with Alexa Fluor 555-tagged donkey-anti-goat (Molecular Probes, 1:1000) and Alexa Fluor 647-tagged donkey-anti-rabbit (Molecular Probes ...
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bioRxiv - Bioengineering 2023Quote: ... 1 x 107 pfu of mRFP-VP26 tagged virions or 1 x 108 of Yellow-Green fluorescent tagged nanoparticles (160 nm, FluoSpheres™ Carboxylate-Modified Microspheres Catalog #F8811, Thermo Fisher Scientific) were added to the dish ...
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bioRxiv - Developmental Biology 2023Quote: ... the samples were washed with 0.1% PBS-TX three times and treated with Alexa Fluor 488-tagged goat-anti-rabbit IgG antibodies (Thermo Fisher, Cat # A-11008), diluted 1:800 in 0.1% PBS-TX for 6–12 h at 4 °C ...
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bioRxiv - Immunology 2021Quote: ... recombinant S was labeled with Alexa Fluor 7647-NHS ester or biotinylated using the EZ-Link Micro NHS-PEG4-Biotinylation Kit (Thermo Fisher Scientific); excess Alexa Fluor 647 and biotin were removed using 7-kDa Zeba desalting columns (Thermo Fisher Scientific).
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bioRxiv - Genetics 2021Quote: ... RNA was base hydrolyzed for 20 min on ice prior to enrichment of biotinylated nascent RNA using DynaBeads MyOne Streptavidin C1 (Invitrogen, Cat #65001). 3’ RNA adapter ligation was carried out off-bead using 25 pmol adapter ...
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bioRxiv - Microbiology 2021Quote: ... cells were stained with the anti-double-stranded (ds) RNA antibody (J2, Scicons) -previously biotinylated with EZ-Link™ Micro NHS-PEG4-Biotinylation Kit (ThermoFisher 21955) to increase specificity and sensitivity of the assay- and with the APC/Cy7 streptavidin antibody (BioLegend ...
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bioRxiv - Developmental Biology 2021Quote: DNA probes for the protein–DNA pull-down assay were generated by PCR using 5’-biotinylated primers and then conjugated to Dynabeads M-280 Streptavidin (Thermo Fisher Scientific). The recombinant proteins synthesized in HEK293T cells were extracted and incubated with the DNA-probe-conjugated beads in 0.5% NP-40 ...
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bioRxiv - Synthetic Biology 2019Quote: Primers were hybridized to a biotinylated target sequence immobilized on streptavidin-coated magnetic beads (Dynabeads MyOne Streptavidin T1, Thermo Fisher, Waltham, MA) by heating to 70°C for five minutes then 55°C for 15 minutes then 25°C for five minutes in RB (1M sodium chloride ...
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bioRxiv - Biophysics 2020Quote: ... and 1% bovine serum albumin (BSA) and then incubated with biotinylated Epidermal Growth Factor (EGF) complexed to Texas Red™ Streptavidin (ThermoFischer/Molecular Probes Cat ...
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bioRxiv - Neuroscience 2020Quote: ... Cells were washed thrice with ice cold PBS2+ and surface BBS-DAT chimeras were labeled with 120μM biotinylated α-bungarotoxin (α-BTX-b, Thermo Fisher) in PBS2+ ...
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bioRxiv - Molecular Biology 2020Quote: ... The biotinylated DNA fragments were applied to the gel-shift assay by using the LightShift Chemiluminescent EMSA Kit (Thermo Fisher Scientific, US) and Chemiluminescent Nucleic Acid Detection Module (Thermo Fisher Scientific ...
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bioRxiv - Cancer Biology 2021Quote: The cell surface of KP4 cells and MYOF-HA expressing HEK293T cells was biotinylated on ice using 0.5 mg/ml of EZ-Link™ Sulfo-NHS-SS-Biotin (Thermo Scientific: 21331) for 30min ...
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bioRxiv - Biophysics 2020Quote: ... The biotinylated surface was then incubated with FluoSpheres™ NeutrAvidin™-marked microspheres (200nm in diameter, fluorescent in yellow-green, ThermoFisher, F8774) with 1-5uL in 150uL PBS per hydrogel ...
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bioRxiv - Immunology 2020Quote: ... The protein concentration was determined and the antibodies biotinylated by incubating for 30 min at room temperature with EZ-Link NHS-PEG4-Biotinylation reagent (Thermo Fisher Scientific) at a 10:1 biotin-to-protein ratio ...
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bioRxiv - Bioengineering 2021Quote: SARS-CoV-2 S was chemically biotinylated using EZ-Link™ NHS-LC-LC-Biotin following manufacturer instructions (Thermo Fisher, Cat#21343). Vero E6 cells (ATCC ...
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bioRxiv - Developmental Biology 2022Quote: ... The DNA was enriched for fragments containing a ligation junction by capturing biotinylated fragments on MyOne Streptavadin C1 magnetic beads (Thermo Scientific, 65001). The DNA ...
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bioRxiv - Immunology 2021Quote: ... 10,000 Hela-hACE2 cells generated by lentivirus transduction of wild-type Hela cells and enriched by fluorescence-activated cell sorting (FACS) using biotinylated SARS-CoV-2 RBD conjugated with streptavidin-Alexa Fluor 647 (ThermoFisher Scientific, S32357) were added to the mixture with 20 µg/ml Dextran (Sigma ...
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bioRxiv - Cell Biology 2021Quote: ... antibodies overnight at 4°C.The sections were then washed with PBS and incubated with a biotinylated secondary antibody for 15 min from a Histostain Plus kit (Invitrogen, CA, USA). The sections were then washed and incubated with 3 ...
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bioRxiv - Immunology 2021Quote: ... Washed yeast libraries were stained in dark and at 4°C with biotinylated antigens pre-conjugated with SA-PE (Streptavidin, R-Phycoerythrin Conjugate, Thermo Fisher Scientific) or SA-APC (Streptavidin ...
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bioRxiv - Biochemistry 2020Quote: ... and then with the appropriate biotinylated secondary antibodies for 30 minutes followed by streptavidin conjugated to Alexa Fluor 594 (ThermoFisher Scientific, UK) for 15 minutes ...
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bioRxiv - Immunology 2022Quote: ... and citrullinated Histone 4 using biotinylated peptides (Cit3-His4 bio: SG[Cit]GKGGKGLGKGGAKRHGSGSK-biotin) on streptavidin-coated high-capacity pre-blocked plates (Thermo Fisher Scientific) and hIgG1 at 5 μg/ml in RIA buffer (1% BSA ...
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bioRxiv - Bioengineering 2024Quote: ... Capture antibodies (provided in Supplementary Table 1) were biotinylated using the EZ-Link micro Sulfo-NHS-biotinylation kit from (Thermo Fisher Scientific). The biotinylated antibodies were then diluted to 10 μg/mL in a 1 % BSA solution and added to the grids for 15 min ...
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bioRxiv - Physiology 2024Quote: OK cells cultured on permeable supports under static or FSS conditions were washed with cold PBS and the apical surface biotinylated with 1 mg/mL EZ-Link Sulfo-NHS-SS-biotin (Thermo Scientific, 21331) in 0.5 mL TEA-buffered saline (TBS ...
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bioRxiv - Microbiology 2024Quote: Nanobodies specific for PfFIKK13149–561 were selected against biotinylated PfFIKK13149–561 immobilised on either Streptavidin-coated magnetic beads (Dynabeads™ M280-Streptavidin, ThermoFisher Scientific) or Pierce™ NeutrAvidin™ Coated plates (#15123 ThermoFisher Scientific) ...
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bioRxiv - Microbiology 2023Quote: ... These PCR products were purified as previously and biotinylated DNA was incubated for 4 hours with streptavidin beads (Dynabeads® kilobaseBINDER™, Invitrogen) to capture only DNA fragments containing the transposon ...
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bioRxiv - Immunology 2023Quote: ... using a Superdex S75 10/300 column in 1XPBS and chemically biotinylated using EZ-Link™ Sulfo-NHS-Biotin (Thermo Fisher Scientific) following manufacturer’s instruction ...
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bioRxiv - Biochemistry 2022Quote: ... and then with the appropriate biotinylated secondary antibodies for 30 minutes followed by streptavidin conjugated to Alexa Fluor 594 (ThermoFisher Scientific, UK) for 15 minutes ...
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bioRxiv - Immunology 2023Quote: ... was biotinylated with 80-fold molar excess of NHS-PEG4-Biotin solution from the EZ-Link™ NHS-PEG4-Biotin kit (ThermoFisher, #A39259). Excess biotin was removed by buffer exchange of protein into sterile 1X PBS using Zeba™ Spin Desalting Columns ...
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bioRxiv - Microbiology 2023Quote: ... LC-MS/MS analysis of the biotinylated peptides was performed on an EASY-nLC 1200 UHPLC connected to an Orbitrap Fusion mass spectrometer (Thermo Fisher Scientific) as described previously67 ...
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bioRxiv - Biochemistry 2023Quote: The ibpA 5’ UTR-gfp mRNA produced from CUGA®7 in vitro transcription kit (Nippon Gene) was attached to a 3’-terminal biotinylated nucleotide using Pierce™ RNA 3’ End Biotinylation kit (Thermo Scientific). The biotin-labeled mRNA (0.1 µM ...
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bioRxiv - Neuroscience 2023Quote: ... holo-Tf was biotinylated for two hours on ice with an EZ-LinkTM Sulfo-NHS-LC-LC-Biotin kit (Thermo Fisher Scientific) and then buffer exchanged into PBS with a 0.5 mL Pierce Protein Concentrator PES column with a 10K MWCO to remove unreacted biotin ...
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bioRxiv - Neuroscience 2024Quote: ... The viruses in the bottom chamber were also detected by dot blot on a nitrocellulose membrane with a biotinylated anti-AAV9 antibody (Thermo Fisher, 7103332100).
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bioRxiv - Neuroscience 2023Quote: ... Tissue was washed in three 10-min PBS exchanges and underwent tyramine signal amplification by treating with PBT comprising 0.005% hydrogen peroxide and 0.5% borate-buffered biotinylated (Sulfo-NHS-LC biotin; 21335, Thermo Fisher Scientific, Waltham, MA) tyramine (T90344 ...
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bioRxiv - Neuroscience 2024Quote: ... Only those sections incubated with biotinylated secondary antibodies were next incubated with Streptavidin-Alexa Fluor 488 conjugate 1:1000 (ThermoFisher scientific, S32354) in PBS with 0.1% Triton X100 ...
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bioRxiv - Biochemistry 2024Quote: ... The desthio-biotinylated mCNPase and hCNPase were immobilised for 2 h at RT on streptavidin-coated flat-bottom 96-well plates (Thermo Fisher Scientific). The wells were washed three times 10 min with PBS and blocked using 5% skim milk in PBS-T for 3 h at RT ...
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bioRxiv - Molecular Biology 2024Quote: DNA probes were biotinylated at the 3’terminal using Biotin 11-dUTP (Thermo: R0081) and terminal deoxynucleotidyl transferase TdT enzyme (Thermo scientific: EP0161) in the presence of CaCl2 containing 5X reaction buffer ...
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bioRxiv - Molecular Biology 2024Quote: ... 50 pmol of synthetic biotinylated RNA or single-stranded DNA (poly(dA) or poly(dG)) were loaded onto the Pierce Streptavidin Magnetic Bead (Thermo Scientific, #20164), and an experimentally normalised 5 µg of human recombinant TG2 was used (this resulted in readily detectable signal after the pull-down).
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bioRxiv - Molecular Biology 2024Quote: ... The final products were size-selected one last time and rRNA depletion was performed using custom-made biotinylated oligos(38) (Table 3) together with MyOne Streptavidin C1 DynaBeads (Thermo Fisher Scientific). Purified RPFs were then used to synthesize cDNA with SuperScript III (Thermo Fisher Scientific) ...