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Citations for Thermo Fisher :
7451 - 7500 of 10000+ citations for Recombinant Human FCGRT & B2M Protein His Avi Strep II tagged Biotinylated since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Developmental Biology 2019Quote: ... The fragment was inserted into pCR-Blunt II-TOPO vector (Invitrogen). From there it was excised using the restriction enzymes AscI and NotI and cloned into a GFP reporter vector (pDVattBR ...
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Gene expression in isolated cowpea (Vigna unguiculata L. Walp) cells from meiosis to seed initiationbioRxiv - Plant Biology 2020Quote: ... and used as donors in LR recombination (LR Clonase II; Invitrogen) with pMDC162 (Curtis and Grossniklaus ...
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bioRxiv - Developmental Biology 2019Quote: ... Cell concentration was determined with an automated counter (Countess II, Invitrogen) with Trypan Blue ...
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bioRxiv - Synthetic Biology 2020Quote: ... The AarI (ER1581) restriction enzyme type II was purchased from ThermoFisher scientific ...
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bioRxiv - Cancer Biology 2019Quote: ... Cell concentration and viability were measured using Countess II (Life Technologies). Live cells were re-suspended with Ba/F3 conditional medium to a concentration of 5 cells/mL ...
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bioRxiv - Molecular Biology 2020Quote: ... equipped with Falcon II direct electron detector (Thermo Fisher Scientific, USA) and Cs image corrector (CEOS ...
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bioRxiv - Molecular Biology 2020Quote: ... random-primed and reserve transcribed using SuperScript II Reverse Transcriptase (Invitrogen). The generated cDNA was 3′ end-adenylated and ligated to Illumina Paired-end sequencing adapters and amplified by PCR using HiSeq SR Cluster Kit v4 cBot (Illumina) ...
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bioRxiv - Developmental Biology 2020Quote: ... 5 μg aRNA was used for reverse transcription (Superscript II, Thermofisher) and library PCR (Phusion DNA polymerase ...
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bioRxiv - Molecular Biology 2021Quote: ... random-primed and reserve transcribed using SuperScript II Reverse Transcriptase (Invitrogen). The generated cDNA was 3′ end-adenylated and ligated to Illumina Paired-end sequencing adapters and amplified by PCR using HiSeq SR Cluster Kit v4 cBot (Illumina) ...
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bioRxiv - Neuroscience 2019Quote: ... and counted using the Countess II cell counter (Thermo Fisher Scientific); cell viability was typically above 80%.
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bioRxiv - Microbiology 2020Quote: ... RNA was first transcribed using SuperScript II Reverse Transcriptase (Thermo Fisher), with oligo dT primers and 50ng of input RNA in each reaction ...
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bioRxiv - Microbiology 2020Quote: ... cDNA was generated using SuperScript II reverse transcriptase (Thermo Fisher Scientific) and oligo(dT ...
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bioRxiv - Systems Biology 2021Quote: ... cell concentration and viability (98 %) was determined using Countess II (Invitrogen) according to the manufacturer’s instructions ...
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bioRxiv - Developmental Biology 2020Quote: ... Cells were counted using Countess II Automated Cell Counter (Life Technologies) per manufacturer’s instructions and seeded onto the micropattern at 132,000 – 263,000 cells/cm2 in mTeSR with 10 μM Rho-associated kinase inhibitor (ROCKi Y-27632 ...
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bioRxiv - Synthetic Biology 2020Quote: ... Gateway pDONR221 Vector and LR Clonase II Enzyme Mix (Life Technologies) and the S ...
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bioRxiv - Developmental Biology 2021Quote: ... A collagenase mix was prepared with collagenase II (17101-015, Gibco) at 1000U/mL ...
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bioRxiv - Cancer Biology 2019Quote: ... staining and Countess II automated cell counter (Life Technologies, Carlsbad, USA).
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bioRxiv - Developmental Biology 2019Quote: ... treatment and cDNA synthesis using SuperScript™ II Reverse Transcriptase (Invitrogen) following the manufacturer’s instructions ...
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bioRxiv - Immunology 2021Quote: ... RNA was reverse transcribed using SuperScript II Reverse Transcriptase (Invitrogen; 18064014). cDNA was diluted 1 μg / 100 ul and qPCR was performed on a CFX96 Instrument (Bio-Rad ...
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bioRxiv - Genetics 2021Quote: ... using the Gateway® BP Clonase II Enzyme mix (Invitrogen, USA). The cloned fragments were verified through sequencing and transferred via Gateway recombination into the pMDC32 vector under the control of 35S promoter ...
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bioRxiv - Cell Biology 2020Quote: ... at different time points by a Cell Counter (Invitrogen, Countess II) using the average of 3 replicates.
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bioRxiv - Physiology 2021Quote: ... After reverse transcription (Super-Script II reverse transcriptase, catalog #18064022, Invitrogen), the absolute number of transcripts in each sample was assessed by quantitative TaqMan PCR (Mm01159196_m1 ...
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bioRxiv - Biochemistry 2021Quote: ... Sf9 cells were grown in Sf-900 II SFM media (Gibco) in shaking cultures at 27 °C ...
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bioRxiv - Biochemistry 2021Quote: ... using the Gateway LR Clonase II enzyme mix (11791020, Thermo Fisher). The open-reading frames in all constructs were verified by Sanger sequencing.
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bioRxiv - Synthetic Biology 2021Quote: ... 20U of type IIS restriction enzyme (BsaI, BpiI, or BsmBI; ThermoFisher), in T4 ligase buffer at a final concentration of 1X and volume of 20ul ...
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bioRxiv - Cancer Biology 2021Quote: ... using the Gateway LR Clonase II enzyme mix (#11791020) from ThermoFisher. TUBA1B was amplified using PCR (donor plasmid ...
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bioRxiv - Systems Biology 2021Quote: ... using TaqMan Universal Master Mix II no UNG kit (ThermoFisher 4440040), TaqMan Gene Expression Assay (ThermoFisher 4331182) ...
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bioRxiv - Cell Biology 2020Quote: ... into pcDNA6.2/C-EmGFP-DEST using Gateway LR Clonase II (ThermoFisher). TagBFP-C-KDEL (# 49150) ...
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bioRxiv - Microbiology 2021Quote: ... and images were acquired usind a Falcon II (Thermo Fisher, USA) direct detector.
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bioRxiv - Microbiology 2020Quote: ... and cDNA was generated by reverse transcription using Superscript II (Invitrogen). Genes in each sample were amplified using gene-specific primers ...
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bioRxiv - Neuroscience 2021Quote: ... Cell viability was confirmed using Countess-II (Invitrogen, Thermofisher, CAT#: AMQAX1000). Next ...
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bioRxiv - Immunology 2021Quote: ... and enzymatically digested with 0.1 mg*ml− 1 Dispase II (Gibco) dissolved in RPMI ...
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bioRxiv - Microbiology 2021Quote: ... or (ii) immersed in RNAlater (Thermo Fisher Scientific, Waltham, MA, USA) and stored at 4° C ...
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bioRxiv - Molecular Biology 2021Quote: ... S2 cells were transfected with cellfectin II reagent (Thermo Fisher Scientific) according to the manufacturer’s protocol ...
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bioRxiv - Immunology 2020Quote: ... RT-qPCR was performed after reverse transcription (Superscript II, Life Technologies) as described previously using SYBR Green ...
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bioRxiv - Microbiology 2021Quote: ... (ii) TOTO™-1 iodide (T3600, Invitrogen™ Thermo Fisher Scientific); a stock solution was prepared in DMSO (at 1 mM ...
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bioRxiv - Immunology 2020Quote: ... as determined using a Countess II automated hemocytometer (Thermo Fisher Scientific), were used to calculate cell numbers for each sample ...
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bioRxiv - Immunology 2021Quote: ... and counted with Countess II Automated Cell Counter (Thermo Fisher Scientific).
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bioRxiv - Developmental Biology 2020Quote: ... Then 50μl of FastDigest Bgl II restriction enzyme (Thermo Fisher Scientific) was added to the reaction tube and incubated overnight at 37°C with shaking at 250rpm ...
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bioRxiv - Genomics 2021Quote: ... For Smartseq3xpress with Platinum II Taq Hot Start DNA polymerase (Invitrogen), PCR mastermix consisted of (1x Platinum II Taq Master Mix ...
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bioRxiv - Genomics 2020Quote: ... and then 2 μl of SuperScript II reverse transcriptase (ThermoFisher Scientific) was added to each reaction ...
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bioRxiv - Genetics 2020Quote: ... Reversed transcription was performed using SuperScript™ II Reverse Transcriptase (Invitrogen) according to the manufacturers protocol (250 - 1000 ng RNA per reaction) ...
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bioRxiv - Cancer Biology 2020Quote: ... cDNA was synthesized with the SuperScript II Reverse Transcriptase system (Invitrogen) or the TaKaRa PrimeScript™ RT Reagent Kit ...
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bioRxiv - Cell Biology 2021Quote: ... 24 mM sodium bicarbonate and 0.5% AlbuMAX II (Gibco #11021-037). The medium was further complemented with 2 mM choline chloride (Sigma #C7527 ...
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bioRxiv - Immunology 2022Quote: ... Reverse transcription (RT) was performed using Superscript II reverse transcriptase (Invitrogen) according to the manufacturer’s recommendations ...
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bioRxiv - Cell Biology 2022Quote: Cells were counted on a Countess II automated cell counter (ThermoFisher) and 12,000 cells were loaded on to one lane of a 10X Chromium microfluidic chip ...
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bioRxiv - Cell Biology 2022Quote: Spdoptera frugiperda (Sf9) cells were cultured in SF900 II SFM (Gibco) at 27°C with orbital shaking at 120 rpm.
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bioRxiv - Molecular Biology 2022Quote: ... The “Phusion Hot Start II DNA Polymerase” (Thermo Fisher, MA, USA) was used for PCR amplification ...
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bioRxiv - Cancer Biology 2022Quote: ... Phusion Hot Start II DNA polymerase (Thermo Scientific, catalog no.: F549L) was used along with the Phusion HF buffer provided ...
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bioRxiv - Biochemistry 2022Quote: ... Samples in 1X formamide loading buffer (Gel loading buffer II – Ambion) were heated for 5 min at 95 ºC prior to analysis ...