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Citations for Thermo Fisher :
7601 - 7650 of 10000+ citations for Parallel Artificial Membrane Permeability Assay PAMPA Kit since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2020Quote: ... These gBlocks were resuspended according to the manufacturer’s protocol and quantified using a Qubit 4 fluorometer with a dsDNA HS assay kit (Q32851 Thermo Fisher), then normalized to 10^8 copies per μL ...
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bioRxiv - Genomics 2021Quote: ... DNA was quantified using a NanoDrop ND8000 spectrophotometer and a Qubit 2.0 fluorometer with the DNA HS assay kit (Life Technologies). DNA quality was examined on agarose gels ...
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bioRxiv - Biochemistry 2020Quote: ... The pellet was re-suspended in 5mL of 1% SDC + 50mM ABC pH 8.5 and protein concentration was measured with Pierce BCA protein assay kit (Thermo Scientific). Samples were then aliquoted into 1.5mL LoBind eppendorf tubes (Sorenson BioScience ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... DNA was quantified using the Qubit HS dsDNA Assay kit on a Qubit 4 fluorometer (Thermo Fisher Scientific, Horsham, UK).
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bioRxiv - Physiology 2021Quote: ... Supernatant (cleared lysates) were used to determine total protein amount using the Pierce BCA Protein Assay Kit (Thermo Fisher Scientific) according to manufacturer’s protocol ...
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bioRxiv - Physiology 2021Quote: ... Supernatant (cleared lysates) were used to determine total protein amount using the Pierce BCA Protein Assay Kit (Thermo Fisher Scientific) according to manufacturer’s protocol ...
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bioRxiv - Physiology 2021Quote: ... Supernatant (cleared lysates) were used to determine total protein amount using the Pierce BCA Protein Assay Kit (Thermo Fisher Scientific) according to manufacturer’s protocol ...
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bioRxiv - Microbiology 2020Quote: ... Purified libraries were quantified with Qubit ssDNA Assay Kit using Invitrogen Qubit 4.0 Fluorometer (Thermo Fisher Scientific, Foster City, CA) and DNA nanoballs were generated by rolling circle amplification of a pool (40 fmol/ reaction) ...
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bioRxiv - Neuroscience 2021Quote: ... Pooled DNA concentration and quality was then assessed by Nanodrop and Qubit dsDNA High Sensitivity assay kit (Thermo Fisher #Q33230). We also ran the pooled PCR products on a 1.0% agarose gel to verify the expected product sizes (∼1-1.1 kb).
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bioRxiv - Cell Biology 2021Quote: ... to the website of online Custom TaqMan® Assay design tool (https://www.thermofisher.com/order/custom-genomic-products/tools/genotyping/) The Custom TaqMan SNP Genotyping Kit (Applied Biosystems #4332073) was ordered and prepared according to manufacturer instructions ...
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bioRxiv - Physiology 2021Quote: ... RNA concentration was quantified using Qubit RNA HS assay kits and a Qubit 2.0 Fluorometer (Thermo Fisher Scientific, Wilmington, DE). Total RNA was stored at -80°C ...
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bioRxiv - Cell Biology 2020Quote: ... Quantification of doublestranded DNA before and after decellularization was done using the Qubit DNA BR Assay kit (Invitrogen, Carlsbad, CA) according to the manufacturer instructions ...
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bioRxiv - Biochemistry 2021Quote: ... Samples were subjected to centrifugation at 14,000 × g and the supernatant was stored at −80 °C or collected for protein quantification using a BCA assay kit according to the manufacturer’s instructions (ThermoFisher 23227). Equal volumes of whole-cell protein extract were subjected to electrophoresis through denaturing 10% bis-Tris polyacrylamide gels and transferred to PVDF membrane ...
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bioRxiv - Neuroscience 2021Quote: ... DNA concentration of each sample was measured using the Qubit® fluorometer with dsDNA BR Assay Kit (Thermo Fisher Scientific).
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bioRxiv - Genomics 2020Quote: ... and the libraries were quantification with Qubit Fluorometer (Qubit™ dsDNA HS Assay Kit, Life Technologies Corporation, Carlsbad, California, USA). Illumina HiSeq 2500 (2 × 150 bp ...
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bioRxiv - Developmental Biology 2021Quote: ... intracellular EdU staining was performed following the manufacturer’s instructions (Click-it plus EdU Alexa fluor 488 flow cytometry assay kit, Invitrogen C10632).
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bioRxiv - Cell Biology 2020Quote: ... Equal loading was ensured by conducting protein concentration measurements with the Pierce BCA Protein Assay Kit (Thermo Scientific, cat# 23225). After SDS-PAGE and western blot transfer ...
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bioRxiv - Evolutionary Biology 2021Quote: ... The 52 sample libraries were subjected to standard normalization for which quantification was executed with the Qubit dsDNA high sensitivity (HS) Assay Kit and Qubit 2.0 Fluorometer (Life Technologies). Dilutions were performed using an EB-Tween solution (EB ...
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bioRxiv - Genetics 2020Quote: ... The concentration and quality of the resulting DNA were checked using the Nanodrop and Qubit dsDNA high sensitivity assay kit (Invitrogen).
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bioRxiv - Bioengineering 2020Quote: ... Toxicity was assessed at 24 h following MENP administration via a CyQUANT™ lactate dehydrogenase (LDH) assay kit (ThermoFisher Scientific), as well as a CellTiter 96® AQueous One MTS assay ...
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bioRxiv - Immunology 2020Quote: ... cells were stained with extracellular antibodies followed by intracellular staining of EdU using click-iT Plus EdU Alexa Fluor 488 Flow Cytometry Assay Kit (Invitrogen), according to the manufacturer’s instructions.
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bioRxiv - Immunology 2020Quote: ... supernatants were discarded and cells were frozen at -80°C before addition of reagents from the CyQUANT Cell Proliferation Assay kit (ThermoFisher) as indicated by the manufacturer.
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bioRxiv - Microbiology 2021Quote: The concentration of total RNA was determined using the Qubit Fluorometer with the Qubit RNA HS Assay Kit (ThermoFisher Q32852). RNA quality was then assessed on an Agilent Bioanalyzer and the RNA 6000 Pico Kit (Agilent 5067-1513) ...
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bioRxiv - Immunology 2020Quote: ... the cytokine levels were determined by Luminex xMAP technology using Human Custom ProcartaPlex Assay kit (Thermofisher, Cat PPX-04-MXRWEYU) in accordance with the manufacturer’s recommendations ...
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bioRxiv - Molecular Biology 2021Quote: ... The total RNA concentration was measured using a Qubit 1.0 Fluorometer and Qubit RNA BR Assay Kit (Thermo Fisher Scientific), whilst RNA quality and integrity was assessed using a NanoDrop™ 2000 spectrophotometer (Thermo Fisher Scientific ...
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bioRxiv - Immunology 2021Quote: ... NKp46+ and stained as indicated by manufacturer’s protocol with Cell Event Caspase 3/7 Green Flow Cytometry Assay Kit (Molecular Probes). All samples were analyzed using BD Canto FACS instrument ((BD Biosciences ...
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bioRxiv - Microbiology 2021Quote: ... RNA concentrations were measured with the Qubit® RNA HS Assay Kit (Qubit®3.0 Fluorometer, Invitrogen, Waltham, MA, USA.). RNA extracts were cleaned with the MEGAclear kit (Thermo Fisher Scientific ...
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bioRxiv - Immunology 2021Quote: ... Thymocytes were stained for extracellular antigens before EdU detection with click-iT Plus EdU Alexa Fluor 488 Flow Cytometry Assay Kit (Invitrogen), following the manufacturer’s protocol.
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bioRxiv - Immunology 2021Quote: ... and size-selected with SPRIselect beads._scRNA/TCR-seq libraries were quantified using a Qubit dsDNA HS Assay kit (Invitrogen) and a HighSensitivity DNA chip run on a Bioanalyzer 2100 system (Agilent) ...
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bioRxiv - Microbiology 2021Quote: ... Quantification of the PCR amplified library was performed with the InvitrogenTM Quant-iTTM PicoGreenTM dsDNA Assay Kit (Cat. No. P7589, ThermoFisher). 16S rRNA gene sequencing was performed on normalized pooled amplicons using the Illumina MiSeq System using a MiSeq reagent kit v2 (300 cycles ...
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bioRxiv - Immunology 2020Quote: ... and the supernatant was collected to determine the protein concentration using the PierceTM BCA protein Assay kit (Thermo Fisher Scientific). 1x sample SDS buffer was added to the supernatant for electrophoresis ...
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bioRxiv - Immunology 2021Quote: ... Double-stranded RNAs (dsRNAs) against relish or GFP used in the luciferase reporter assay were synthesized using MEGAscript T7 kit (Invitrogen). Primers used for PCR amplification are listed in Supplementary Table 1.
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bioRxiv - Immunology 2021Quote: Protein Digestion and Tandem Mass Tag (TMT) labeling: The Pierce detergent compatible Bradford assay kit from Thermo Fisher (Winnipeg, MB) was used to determine the protein concentration ...
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bioRxiv - Immunology 2020Quote: ... Quality and amount of RNA were determined using the Qubit RNA BR Assay Kit with Qubit 2.0 (Thermo Fisher Scientific) and Agilent RNA 6000 Nano Kit on the Agilent 2100 Bioanalyzer ...
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bioRxiv - Immunology 2020Quote: ... Quality and amount of RNA were determined using the Qubit RNA BR Assay Kit with Qubit 2.0 (Thermo Fisher Scientific) and Agilent RNA 6000 Nano Kit on the Agilent 2100 Bioanalyzer ...
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bioRxiv - Genomics 2021Quote: ... Resulting cDNA (average 45 ng/replicate) was checked for quality by Qubit dsDNA HS Assay Kit (Thermo Fisher Scientific #Q32854) and Agilent Bioanalyzer High Sensitivity Kit (Agilent #5067-4626) ...
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bioRxiv - Genomics 2020Quote: ... genomic DNA samples were serially diluted and then quantified using the Qubit™ dsDNA BR Assay Kit (Invitrogen, Carlsbad, CA) and the Qubit™ dsDNA HS Assay Kit (Invitrogen ...
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bioRxiv - Genomics 2021Quote: ... Apoptosis staining was performed using the CellEvent Caspase-3/7 Green Flow Cytometry Assay Kit (Thermo Fisher Scientific, Waltham, MA). Stained cell suspensions were measured with the BD LSRFortessa Cell Analyzer (BD Biosciences ...
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bioRxiv - Genomics 2020Quote: ... and quantified the purified amplicon using the Qubit dsDNA High Sensitivity assay kit on a Qubit 4.0 instrument (Life Technologies). We estimated amplicon fragment sizes on a LabChip GX Touch (Perkin Elmer ...
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bioRxiv - Genomics 2020Quote: ... Libraries were quantified using the Qubit dsDNA High Sensitivity assay kit on a Qubit 4.0 instrument (Life Technologies, California, USA). We analysed the fragment sizes using a LabChip GX Touch (Perkin Elmer ...
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bioRxiv - Microbiology 2020Quote: ... The concentration of double strand cDNA was evaluated (Table 1) using the Qubit® dsDNA HS Assay Kit (Life Technologies). For six DNA samples ...
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bioRxiv - Synthetic Biology 2022Quote: Total protein concentration in the purified samples was determined with the Pierce Rapid Gold BCA Protein Assay kit (ThermoFisher A53225) by comparing absorbance signal at 480 nm to a BSA standard curve ranging from 0-2000 μg/ml using manufacturer’s instructions ...
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Neuron-astrocyte metabolic coupling facilitates spinal plasticity and maintenance of persistent painbioRxiv - Neuroscience 2022Quote: Quality control: The amplified cDNA was subjected to concentration measurement using the Qubit dsDNA HS Assay kit (Thermo Fisher, Q32854) and a quality check using a bioanalyzer (Agilent High Sensitivity DNA Kit ...
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bioRxiv - Cell Biology 2023Quote: The oskar RNA used for the reconstitution assay was uncapped and synthesized in vitro using the MEGAscript T7 Transcription Kit (Ambion). The RNA was transcribed from a linearized plasmid DNA template containing an ~529-nt region of the osk 3’UTR previously defined as region 2+3 that includes the oocyte entry signal (OES ...
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bioRxiv - Molecular Biology 2022Quote: ... Immunoprecipitation was performed on 150 μg of chromatin quantified using the Pierce™ BCA Protein Assay Kit (Thermo Fisher Scientific) with 60 μl of Protein-A/G Dynabeads and 3.5 μl of anti-GFP (Thermo Fisher #A11122 ...
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bioRxiv - Neuroscience 2023Quote: ... proteins were extracted in cold RIPA lysis buffer and supernatant was used to determine protein concentration using the Pierce BCA protein assay kit (Thermofisher). 50 μg protein was loaded on Mini-PROTEAN TGX Stain-Free Gels before transfer to Nitrocellulose membranes (Biorad) ...
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bioRxiv - Microbiology 2023Quote: ... Extraction blanks were run with each round of DNA extractions and all returned no detectable nucleic acids using the maximum amount of blank sample (20μL) via the Qubit dsDNA High Sensitivity assay kit (ThermoFisher Scientific). For all 78 samples ...
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bioRxiv - Microbiology 2023Quote: ... The library was quantified using a Qubit™ double stranded DNA (High Sensitivity) Assay Kit (#Q32866, Life Technologies Ltd, UK) with the Qubit® 2.0 Fluorometer (Life Technologies Ltd ...
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bioRxiv - Neuroscience 2023Quote: ... Supernatant was collected and protein concentration determined by Pierce™ BCA Protein Assay Kit (Thermo Fisher Sci., Cat. no: 23225).
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bioRxiv - Neuroscience 2023Quote: ... The supernatant was collected and protein concentration was quantified using a Pierce BCA protein assay kit (Thermo Fisher Scientific, USA) according to manufacturer instructions ...