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Citations for Thermo Fisher :
7551 - 7600 of 10000+ citations for Parallel Artificial Membrane Permeability Assay PAMPA Kit since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genomics 2020Quote: ... and the concentration of RNA samples was measured by Qubit RNA HS Assay Kit (Thermo Fisher Scientific, Waltham, MA, USA). DNA-depleted and purified RNA was used to construct the single-stranded circular DNA library with MGIEasy RNA Library preparation reagent set (MGI ...
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bioRxiv - Genomics 2020Quote: ... We then amplified the library using Hi-fidelity KAPA PCR kit (KAPA) for 6 cycles and quantified the library using Qubit high sensitivity DNA assay (Thermofisher). We performed QC using bioanalyzer (Agilent ...
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bioRxiv - Genomics 2020Quote: ... We then amplified the library using Hi-fidelity KAPA PCR kit (KAPA) for 6 cycles and quantified the library using Qubit high sensitivity DNA assay (Thermofisher). We performed QC using bioanalyzer (Agilent ...
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bioRxiv - Neuroscience 2021Quote: ... OCR measurements were normalised to cell number which was quantified using the CyQUANT™ Cell Proliferation Assay Kit (ThermoFisher Scientific). Data was analysed using Wave software (Agilent).
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bioRxiv - Neuroscience 2020Quote: ... samples were fixed with 4% paraformaldehyde and permeabilized with 0.25% Triton X-100 for 15 minutes and then stained for TUNEL following manufacturer’s instructions (Click-iT TUNEL assay kit from Life Technologies). Cells were blocked with 3% BSA for 1 hour and then incubated in primary and secondaries ...
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bioRxiv - Microbiology 2020Quote: ... Enzyme-linked immunosorbent assays (ELISAs) for human IL-8 were performed using the Human IL-8 ELISA Kit (ThermoFisher SCIENTIFIC) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2021Quote: ... according to the manufacturer’s protocol and normalized to total protein concentrations using the Pierce BCA Protein Assay kit (Thermo Scientific).
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bioRxiv - Cell Biology 2021Quote: ... The protein concentration of clarified cell lysate was then measured using the BCA protein assay kit (Thermo Fisher, Cat# 23227). 5 ∼ 20 μg of total protein per lane was separated by SDS-PAGE gel and transferred to PVDF membrane ...
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bioRxiv - Cell Biology 2021Quote: ... The concentration of sc/snRNA-seq sequencing libraries was quantified by Qubit™ ssDNA Assay Kit (Thermo Fisher Scientific, #Q10212). The resulting libraries were sequenced using a DIPSEQ T1 or DIPSEQ T7 sequencers at the China National GeneBank (Shenzhen ...
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bioRxiv - Microbiology 2020Quote: ... with minor alterations including no vigorous mixing steps and final elution in 100μl of nuclease free water and quantified using a Qubit and the HS (high sensitivity) dsDNA assay kit (Thermofisher Scientific), following the manufacturer’s instructions ...
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bioRxiv - Microbiology 2020Quote: ... checked on Agilent DNA1000 chip on Bioanalyzer2100 and quantified by Qubit Fluorometer 2.0 using Qubit dsDNA HS Assay kit (Life Technologies). After obtaining the Qubit concentration for the library and the mean peak size from Bioanalyzer profile ...
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bioRxiv - Cancer Biology 2020Quote: ... organoids were incubated with 2 mM Calcein-AM and 4 mM Ethidium homodimer-1 (LIVE/DEAD assay kit, Molecular Probes) for up to 6h ...
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bioRxiv - Neuroscience 2021Quote: ... Protein from each well was extracted with 0.1% NP-40 PBS solution and quantified with BCA protein assay kit (Thermo Fisher), and data were normalized from protein concentration ...
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bioRxiv - Neuroscience 2020Quote: ... protein concentration in each sample was determined by using the Pierce™ BCA Protein Assay Kit (Thermo Fisher Scientific, 23225). 75µg protein per condition was transferred into new microcentrifuge tubes and 5 µL of the 200 mM TCEP was added to reduce the cysteine residues and the samples were then incubated at 55°C for 1-h ...
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bioRxiv - Immunology 2020Quote: The library pool was quantified using the Quant-iT™ PicoGreen® double-stranded DNA Assay Kit (Thermo Fisher Scientific) to ensure adequate DNA yield for sequencing using the Illumina MiSeq (Illumina ...
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bioRxiv - Microbiology 2021Quote: PG-degrading activity of human and hen egg white lysozyme was determined on a fluorescein-labelled Micrococcus lysodeiticus cell wall substrate using the EnzChek Lysozyme Assay Kit (ThermoFisher), in which one unit is defined as the amount of enzyme required to produce a 0.001 units per minute change in the absorbance at 450 nm of at pH 6.24 and 25°C ...
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bioRxiv - Microbiology 2021Quote: ... DNA quantity for short read sequencing was measured using Quant-iT™ PicoGreen™ dsDNA Assay Kit (ThermoFisher Scientific, USA). DNA was stored in −20°C until library preparation.
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bioRxiv - Molecular Biology 2022Quote: ... 40ul of sample was recovered and 2ul were used to measure the concentration using Qubit dsDNA HS Assay kit (ThermoFisher). The rest of the sample was used for P7 tagmentation by mixing the following reaction ...
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bioRxiv - Microbiology 2022Quote: ... trichloroacetic acid was performed as described previously 37 and protein concentration was determined by BCA protein assay kit (Thermo scientific). Protein samples were separated by 4– 12% gradient SDS-PAGE (GenScript ...
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bioRxiv - Cell Biology 2022Quote: ... Total protein concentrations in cell lysates were measured using the Pierce™ bicinchoninic acid (BCA) assay kit (23225, Thermo Fisher). 10-15 μg of proteins were separated by SDS-PAGE and immunoblotted using the following antibodies ...
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bioRxiv - Developmental Biology 2022Quote: ... with High Sensitivity D5000 ScreenTape and library concentrations were measured with Qubit dsDNA High Sensitivity assay kit (Thermo Fisher Scientific). The eight libraries (one for each mouse ...
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bioRxiv - Microbiology 2022Quote: ... DNA was eluted into 18 µL TE buffer and quantified using the Qubit dsDNA HS Assay kit (Thermo Fisher Scientific). This in vitro degraded DNA was then used to generate DNA sequencing libraries.
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bioRxiv - Microbiology 2022Quote: ... DNA was eluted into 18 µL TE buffer and quantified using the Qubit dsDNA HS Assay kit (Thermo Fisher Scientific). DNA isolated from wild-type Serratia with CRISPR targeting (pPF1467 ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... AChE activity was normalized by protein concentration as determined by a Coomassie (Bradford) protein assay kit (Thermo Scientific, Waltham, MA) and compared to 0.5% DMSO exposed samples (set at 100% activity) ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... ChE activity was normalized by protein concentration as determined by a Coomassie (Bradford) protein assay kit (Thermo Scientific, Waltham, MA) and compared to vehicle-exposed samples (set at 100% activity) ...
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bioRxiv - Genetics 2022Quote: ... pH 9.0) and the concentration of the lysate was determined by using a Pierce BCA Protein Assay Kit (ThermoFisher Scientific). Subsequently ...
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bioRxiv - Genomics 2022Quote: ... and quantified using a Qubit™ dsDNA HS Assay Kit on a Qubit™ 3 Fluorometer (Thermo Fisher Scientific, 2017) using the manufacturers’ respective protocols ...
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bioRxiv - Immunology 2022Quote: In situ-hybridization on lungs was performed as previously described (71) using the ViewRNA™ ISH Tissue Assay Kit (Invitrogen by Thermo Fisher Scientific ...
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bioRxiv - Biochemistry 2022Quote: ... Lysates were clarified by centrifugation at 20,800 g at 4°C for 10 min and supernatant protein concentrations quantified using the Bradford assay kit (ThermoFisher, #23236)
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bioRxiv - Plant Biology 2020Quote: ... Stranded RNAseq libraries were constructed using 2ug of total RNA quantified using the Qubit RNA HS assay kit (Invitrogen, USA) with the Illumina TruSeq Stranded Total RNA with Ribo-Zero Plant (Illumina ...
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bioRxiv - Cell Biology 2020Quote: ... The values obtained were normalised to total mg DNA per well as measured by Quant-iT(tm) PicoGreen(tm) dsDNA Assay Kit (Invitrogen). For mouse adipocytes ...
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bioRxiv - Genetics 2020Quote: ... amplicons were quantified using a Qubit dsDNA HS kit or BR assay (Ion Torrent and MinION, respectively; ThermoFisher Scientific, USA). For Ion Torrent ...
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bioRxiv - Evolutionary Biology 2020Quote: ... DNA was quantified with a Qubit fluorometer using the Qubit™ dsDNA BR Assay kit (Thermo Fisher Scientific, MA, USA) prior library preparation ...
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bioRxiv - Microbiology 2020Quote: ... The quantity and quality of the RNA was assessed using the Qubit (Qubit RNA BR Assay Kit, Invitrogen, CA, USA) and the Agilent 2100 Bioanalyzer system (Agilent RNA 6000 Nano Kit ...
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bioRxiv - Genetics 2020Quote: ... EdU was added to the SC culture medium with a final concentration of 10 mM and the incorporation assay was detected by Click-iT EdU kit (Invitrogen) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2020Quote: ... DNA purity and concentration were assessed using a spectrophotometer (NanoDrop™) and Qubit® dsDNA HS Assay Kit (Invitrogen™) #Q32854) ...
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bioRxiv - Molecular Biology 2020Quote: ... cells were processed following Click-iT™ EdU Alexa Fluor™ 488 Flow Cytometry Assay Kit manufacturer’s manual (ThermoFisher #C10420), but instead of saponin-based permeabilization and wash reagent ...
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bioRxiv - Molecular Biology 2020Quote: ... The ChIP assay including crosslinking and harvest of cells were carried out using the Pierce Magnetic ChIP Kit (Thermo Scientific) according to the manufacturer’s protocol except that sonication was carried out on a Covaris S2 ultrasonicator (settings ...
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bioRxiv - Neuroscience 2021Quote: ... Total protein content of the samples was measured with the bicinchoninic acid assay (kit from Pierce, Thermofisher Scientific, Göteborg, Sweden). Activity was expressed as mU/mg protein ...
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bioRxiv - Neuroscience 2020Quote: ... Cytokine levels were normalized to the total protein concentration in the lysate measured with BCA protein assay kit (Thermo Scientific). NO levels were analyzed using Griess Reagent Kit for Nitrite Determination (G-7921 ...
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bioRxiv - Cancer Biology 2020Quote: ... The cell culture supernatant was collected and used for quantifying IL-8 secretion using the ELISA assay kit (# 88-8086-88, ThermoFisher). The assay was performed following the manufacturer’s protocol ...
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bioRxiv - Immunology 2020Quote: ... EdU and BrdU incorporation into DNA was assayed using the Click-iT Plus EdU Alexa Fluor 647 Flow Cytometry Assay Kit (Invitrogen) and FITC BrdU Flow Kit (BD) ...
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bioRxiv - Cell Biology 2020Quote: De novo protein synthesis was measured using Click-IT HPG Alexa Fluor 488 protein synthesis assay kit (Thermo Fisher Scientific) as per company instructions ...
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bioRxiv - Immunology 2021Quote: ... RNA concentration was measured using the Qubit RNA Assay Kit in a Qubit 2.0 Flurometer (Life Technologies, Carlsbad, CA, USA). RNA integrity was assessed using the RNA Nano 6000 Assay Kit of the Agilent Bioanalyzer 2100 system (Agilent Technologies ...
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bioRxiv - Physiology 2021Quote: ... In-situ hybridization imaging of RAGE was conducted using the QuantiGene ViewRNA ISH Tissue 1-plex Assay kit and procedure from Affymetrix. We performed the assay using a modified 1-plex protocol for Fast Blue Detection using Type 1 Mouse AGER probe and Type 1 Mouse GAPDH probe (Affymetrix) ...
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bioRxiv - Molecular Biology 2020Quote: ... The DNA concentration was measured on a Qubit 3.0 fluorometer with the Qubit dsDNA HS assay kit (Thermo Fisher Scientific) and the DNA quality was measured by gel electrophoresis on an Agilent 2200 Tapestation using Genomic Screentapes (Agilent Technologies).
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bioRxiv - Neuroscience 2020Quote: hiPSC-derived neuron and astrocyte co-cultures were loaded with Fluo 4 direct (calcium assay kit by Thermo Fisher Scientific) for 30 minutes in 37 °C ...
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bioRxiv - Genomics 2020Quote: ... The concentration of each DNA extract was determined using a Qubit dsDNA HS Assay Kit (Life Technologies, Carlsbad, CA, USA). We aimed to sequence each pool to 50X as per recommendations of Schlötterer et al ...
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bioRxiv - Microbiology 2021Quote: ... The concentration of dsDNA in each miniprep was measured using the Qubit-4 fluorometer and the associated dsDNA high sensitivity assay kit (Invitrogen). Then ...
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bioRxiv - Microbiology 2021Quote: ... We measured the concentration of dsDNA in each miniprep using the Qubit-4 fluorometer and the associated dsDNA high sensitivity assay kit (Invitrogen). For each sample with a SpyCas9-expressing plasmid ...