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Citations for Thermo Fisher :
7401 - 7450 of 10000+ citations for Parallel Artificial Membrane Permeability Assay PAMPA Kit since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2024Quote: ... transferred to nitrocellulose membranes (Thermo Fisher, LC2001), and probed with rat anti-HA-peroxidase 1:1000 (Roche 12013819001 ...
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bioRxiv - Developmental Biology 2024Quote: ... 0.2 µm PVDF membrane (Thermo Fisher, 88520) was first equilibrated for 30 seconds in methanol followed by a 20-minute equilibration in 1X transfer buffer (100mL 10X running buffer ...
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bioRxiv - Bioengineering 2024Quote: ... Transfer to a nitrocellulose membrane (Invitrogen IB301002) was performed with the iBlot dry transfer system ...
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bioRxiv - Cancer Biology 2024Quote: ... or Nitrocellulose membrane 0.45 μm (Thermo Fisher).
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bioRxiv - Cell Biology 2024Quote: ... and blotted onto a PVDF membrane (Invitrogen). Following are the antibodies used and the relative dilutions ...
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bioRxiv - Cancer Biology 2024Quote: ... and transferred onto PVDF membranes (Invitrogen, #IB34001). The membranes were then incubated with antibodies diluted in 2% Bovine Serum Albumin (BSA ...
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bioRxiv - Cell Biology 2024Quote: ... or nitrocellulose membranes (Thermo Fisher Scientific, #88018) and analyzed using the indicated primary antibodies and appropriate IRDye-conjugated secondary antibodies ...
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bioRxiv - Neuroscience 2021Quote: ... Membrane proteins were extracted using the Mem-PER Plus Membrane Protein Extraction Kit according to the manufacturer’s instructions (Cat# 89842; ThermoFisher Scientific, Waltham, MA), using a mixture of protease and phosphatase inhibitors (10 μl/ml ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: Membrane-associated proteins were isolated from approximately 100 mg of uterine myometrial tissue using Mem-PER Plus Membrane Protein Extraction Kit (catalog# 89842, ThermoFisher Scientific, Inc.) following manufacturer’s instructions and subjected to immunoblotting with appropriate positive and negative controls (Cat# ...
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bioRxiv - Physiology 2022Quote: ... Extraction of cell membrane and cytoskeleton fractions was carried out using the Subcellular Protein Fractionation Kit for Cultured Cells (Thermo Scientific, #78840) in line with the manual.
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bioRxiv - Plant Biology 2021Quote: ... Equal loading was checked by taking images of the stained PVDF membranes with Pierce Reversible Protein Stain Kit (#24585, Thermo Fisher Scientific).
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bioRxiv - Physiology 2022Quote: ... Protein abundance was normalized to total protein using Pierce™ Reversible Protein Stain Kit for PVDF Membranes (cat 24585; Thermo Fisher Scientific).
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bioRxiv - Molecular Biology 2023Quote: ... The membrane was then washed three times with TBST and the bands were visualized using a chemiluminescent kit for HRP (Thermo Scientific, 34075) according to the manufacturer’s instructions ...
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bioRxiv - Developmental Biology 2022Quote: CM monolayers were loaded with 0.7 times the manufacturer’s suggested amount of the voltage-sensitive dye Fluovolt (FluoVolt™ Membrane Potential Kit (Thermo Fisher Scientific, #F10488)) after three repeated wash steps with Hank’s Balanced Salt Solution (HBSS ...
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bioRxiv - Developmental Biology 2022Quote: ... A loading control was performed by total protein staining using Pierce Reversible Protein Stain Kit for Nitrocellulose Membranes (Thermo Fisher Scientific, 24580). For blocking ...
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bioRxiv - Cell Biology 2021Quote: ... Probes used: Neurochondrin (Assay ID Dm02137509_s1) and Rlp32 (Assay ID Dm02151827_g1) (Thermo Fisher Scientific).
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bioRxiv - Cancer Biology 2020Quote: ... Protein concentrations were measured by bicinchoninic acid assay (BCA protein assay, Thermo Fisher Scientific). Glut-1 ...
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bioRxiv - Neuroscience 2020Quote: ... Total protein content was determined by Bradford assay or Pierce™ 660nm assay (ThermoFisher). Samples from individual animals were run separately and were not pooled.
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bioRxiv - Microbiology 2023Quote: ... miR-19a (Assay ID: 000395) and miR-92a (Assay ID:000430) (Applied Biosystems, USA), as per manufacturer’s protocol ...
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bioRxiv - Neuroscience 2022Quote: ... Total protein content was determined by Bradford assay or Pierce 660 nm assay (ThermoFisher). Samples from individual animals were analysed separately and were not pooled.
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bioRxiv - Bioengineering 2024Quote: Metabolic Viability Assay Analysis: At selected time points an Alamar Blue assay (Invitrogen, UK) was performed according to the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2024Quote: ... Protein concentration was determined using bicinchoninic acid assay (BCA) assay (#23225, Thermo Fisher Scientific). Equal amounts of protein were separated by 4-12% NuPAGE (Invitrogen ...
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bioRxiv - Genetics 2023Quote: ... Protein concentration was determined by bicinchoninic acid assay (BCA) protein assay (#23227, Life Technologies). Samples were separated using precast 4-12% Bis-Tris polyacrylamide gels (ThermoFisher Scientific ...
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bioRxiv - Cancer Biology 2023Quote: ... Protein concentration was determined by bicinchroninic acid assay (BCA) protein assay (#23227, Life Technologies), after which equal amounts of protein were loaded and separated by polyacrylamide gel electrophoresis ...
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bioRxiv - Genomics 2023Quote: ... DNA sample quantification was done by Qubit assay (dsDNA HR assay, Thermo Fisher Scientific) and quality was determined by Nanodrop A260/280 and A260/230 ratios ...
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bioRxiv - Molecular Biology 2023Quote: ... DNA concentration was measured using the Qubit assay (Qubit dsDNA High Sensitivity assay, ThermoFisher). The ChIP reaction was assembled as follows ...
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bioRxiv - Immunology 2024Quote: The LDH viability assay was performed using Invitrogen CyQUANT LDH Cytotoxicity Assay (Fisher Scientific) according to the manufacturers instructions ...
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bioRxiv - Cancer Biology 2024Quote: Cell viability assay was performed with the MTT colorimetric assay (Thermo Fisher Scientific, M6494). Cell cycle analysis used PI staining (Thermo Fisher Scientific ...
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bioRxiv - Neuroscience 2024Quote: ... 0.5 µl TaqMan Assay RPL13A (Assay-ID: Hs04194366_g1 VIC-MGB, Applied Biosystems; Darmstadt, Germany), 0.5 µl of TaqMan Assay Netrin 1 (Assay-ID ...
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bioRxiv - Microbiology 2020Quote: ... Plant and soil DNA concentrations were determined using a Qubit™ fluorometer with Quant-iT dsDNA HS Assay Kit (Invitrogen). The DNA samples were stored at -20°C until further analysis.
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bioRxiv - Microbiology 2020Quote: ... Measurement of the DNA yield was attempted with Quant-iT PicoGreen dsDNA Assay Kit (Molecular Probes, Life Technologies, Eugene, Or) but accurate quantification was not possible for many of the small samples due to results close to the background fluorescence in the blank controls ...
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bioRxiv - Microbiology 2020Quote: ... Measurement of the DNA yield was attempted with Quant-iT PicoGreen dsDNA Assay Kit (Molecular Probes, Life Technologies, Eugene, Or) but accurate quantification was not possible for many of the small samples due to results close to the background fluorescence in the blank controls ...
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bioRxiv - Biophysics 2021Quote: ... Total protein concentration in the BAL fluid was measured using the Pierce™ BCA assay kit (Thermo Scientific, Rockford, IL), as previously described.6
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bioRxiv - Cancer Biology 2021Quote: ... Bound UHMW DNA was resuspended in elution buffer (EB) and quantified with Qubit dsDNA BR assay kit (ThermoFisher Scientific, Q32850). The final DNA concentration was initially too high (280 ng/µl) ...
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bioRxiv - Cancer Biology 2021Quote: ... harvested and processed using the Click-iT™ EdU Alexa Fluor™ 647 Flow Cytometry Assay Kit (ThermoFisher Scientific #C10424) following manufacturer instructions ...
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bioRxiv - Cell Biology 2020Quote: Cell proliferation was assessed using the Click-iT™ Plus EdU Alexa Fluor™ 488 Flow Cytometry Assay Kit (ThermoFisher) following the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2020Quote: Cell proliferation was assessed using the Click-iT™ Plus EdU Alexa Fluor™ 488 Flow Cytometry Assay Kit (ThermoFisher) as described above ...
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bioRxiv - Cell Biology 2020Quote: Total RNA was obtained from cells isolated from organoid assays using the PicoPure RNA Isolation Kit (Cat#KIT0204; Applied Biosystems), following the manufacturers’ protocols ...
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bioRxiv - Cell Biology 2020Quote: ... The concentration and quality of extracted RNA were evaluated using the Quant-iT RiboGreen RNA Assay Kit (Thermo Fisher Scientific) and the RNA 6000 Pico Chip (Agilent Technologies) ...
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bioRxiv - Cell Biology 2020Quote: ... Total concentration of protein in lysate was quantified using the Pierce™ BCA Protein Assay Kit (Thermo Fisher Scientific, 23225) measured at 562 nm and compared to a standard curve ...
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bioRxiv - Molecular Biology 2021Quote: ... The concentrations of libraries were quantified using Qubit dsDNA HS Assay Kit and Qubit 2.0 Fluorometer (Thermo Fisher Scientific, USA) and size validated using Agilent 2100 Bioanalyzer (Agilent ...
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bioRxiv - Developmental Biology 2021Quote: ... RNA integrity was determined by agarose electrophoresis and concentration measured using Qubit RNA Assay Kit in a Qubit 2.0 Fluorometer (Life Technologies, Invitrogen). cDNA libraries were synthetized from 1 µg of total RNA and sequenced using a HiSeq-3000 platform (Illumina ...
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bioRxiv - Developmental Biology 2021Quote: ... EdU incorporation was also analyzed using Click-iT™ EdU Alexa Fluor™ 488 Flow Cytometry Assay Kit (Invitrogen, C10420) after 24h incubation with 10μM EdU ...
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bioRxiv - Developmental Biology 2020Quote: Cell cycle phase distribution analysis was conducted using Click-iT EdU Alexa Fluor 647 Flow Cytometry Assay Kit (Life Technologies) and FxCycle Violet (Life Technologies ...
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bioRxiv - Developmental Biology 2020Quote: ... EdU incorporation assays were performed with the Click-iT® EdU Alexa Fluor® 647 Imaging Kit (C10339, Life Technologies) according to the manufacturer’s protocol ...
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bioRxiv - Genomics 2020Quote: ... μl of DNA samples were normalized to a concentration of 10 ng/μl using Quant-iT Picogreen dsDNA Assay Kit (Invitrogen) for precision quantification ...
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bioRxiv - Microbiology 2022Quote: ... DNA extracted from seed samples was quantified with Qubit™dsDNA BR Assay Kit (ThermoFisher Scientific, Waltham, MA, United States) and verified with Polymerase Chain Reaction (PCR) ...
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bioRxiv - Cell Biology 2022Quote: ... Supernatants and lysates were assayed for LDH using an LDH colorimetric assay kit as per the manufacturer’s instructions (Invitrogen C20300). hMDMs were seeded at 500 000 cells per well in 12-well plates and differentiated as described above ...
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bioRxiv - Cell Biology 2022Quote: ... The cells were washed once with PBS then lysed in lysis buffer provided in the LDH assay kit (Invitrogen C20300). Supernatants and lysates were assayed for LDH using an LDH colorimetric assay kit as per the manufacturer’s instructions (Invitrogen C20300) ...
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bioRxiv - Neuroscience 2020Quote: ... The cDNA concentration was measured using a Qubit DNA Assay Kit in a Qubit 3.0 Fluorometer (Life Technologies, CA, USA). DNA fragment size was tested using an Agilent Bioanalyzer 2100 system (Agilent Technologies ...