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Citations for Thermo Fisher :
7601 - 7650 of 10000+ citations for Biotin Z Antibody Internalization Kit rabbit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2022Quote: ... the sections were incubated with secondary antibody 1:1000 (Alexa Flour 594 gaot anti-rabbit IgG and Alexa Flour 488 goat anti-mouse IgG (Invitrogen #A11037 and #A10680 respectively)) for 1 h at room temperature followed by washing thrice ...
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bioRxiv - Neuroscience 2020Quote: ... Sections were then incubated with one or more of the following primary antibodies (rabbit anti-TH, 1:2000, AB152, EMD Millipore; guinea pig anti-VGLUT2, AB2251, EMD Millipore; chicken anti-GFP, A10262, Invitrogen, Carlsbad, CA) in blocking buffer (overnight ...
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bioRxiv - Microbiology 2021Quote: ... followed by incubation with 1:500 dilution of goat anti-rabbit Alexa Fluor 488 and 1:500 dilution of goat anti-mouse Alexa Fluor 647 antibodies (Thermo Fisher Scientific, Massachusetts, USA) in antibody dilution buffer at room temp for 1 hour ...
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bioRxiv - Biochemistry 2020Quote: ... Samples were then washed three times in PBS and incubated with secondary antibodies diluted in blocking buffer for an hour at room temperature: anti-rabbit Alexa Fluor® 488 nm (ThermoFisher Scientific, Loughborough, UK) and anti-mouse Alexa Fluor® 594 nm (ThermoFisher Scientific ...
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bioRxiv - Cell Biology 2021Quote: ... Secondary antibodies against rabbit primary antibodies were Alexa Fluor 488- (1:400 dilution) or Alexa Fluor 594-coupled (1:200 dilution) anti-rabbit IgG (Invitrogen, #A11034 or #A11037, respectively). Alexa Fluor 488- and Alexa Fluor 594-coupled phalloidin were obtained from Invitrogen (#A12379 and #A12381 ...
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bioRxiv - Neuroscience 2020Quote: ... ∼24.4°C) in a secondary antibody coupled to a green fluorophore (1:200; donkey anti-rabbit AF488, Cat#A21206, Thermo Fisher Scientific, Cambridge, MA). For cholinergic neuronal labeling in vGluT2-tdTomato/GAD67-GFP crossed tissue (Fig ...
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bioRxiv - Neuroscience 2021Quote: ... and then were immune-stained with Alexa Fluor 488 conjugated anti-rabbit secondary antibody (1:200, from donkey, Thermo Fisher Scentific, A-21206, USA). Confocal images were taken under confocal microscopy (Cell Observer SD ...
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bioRxiv - Neuroscience 2021Quote: ... Secondary antibodies were incubated in 1% NGS in wash buffers at 4°C with agitation with a 1:400 dilution at the same durations as the first antibodies (goat anti-mouse-Alexafluor-488, A-11017; goat anti-rabbit-Alexafluor-555, A-21430; ThermoFisher Scientific/Invitrogen, MA, USA). Then ...
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bioRxiv - Neuroscience 2021Quote: ... The following secondary antibodies were applied: Alexa Fluor® 488 and 647 anti-mouse or anti-rabbit IgG (1:1000; Invitrogen, Eugene, OR, USA). After immunostaining ...
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bioRxiv - Developmental Biology 2021Quote: ... incubated with AlexaFluor-555 Goat-anti-Mouse and AlexaFluor-647 Goat-anti-Rabbit Secondary Antibodies (Thermo Fisher Scientific, Waltham, MA, both at 1:500) for 1 hour at room temperature ...
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bioRxiv - Cancer Biology 2021Quote: ... cells were incubated with secondary antibodies (goat anti-mouse Alexa Fluor 568 or goat anti-rabbit Alexa Fluor 488; Thermo Fisher Scientific, 1:500) for 1 h at room temperature ...
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bioRxiv - Developmental Biology 2022Quote: ... Embryos were then washed 4× 2 h in PBT and incubated overnight in PBT supplemented with 1:500 goat anti-mouse or goat anti-rabbit secondary antibodies coupled either to Alexa Fluor 488 or 568 (Invitrogen – Thermo Fisher Scientific, Waltham, MA). Embryos were then washed 4× 2 h in PBT and gradually dehydrated in methanol ...
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bioRxiv - Developmental Biology 2022Quote: ... The presence of relevant proteins was visualized using Alexa Fluor secondary antibodies (donkey anti-mouse AF488, anti-rabbit AF555, A-31572, Thermofisher and anti-goat AF647).
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bioRxiv - Neuroscience 2022Quote: ... and then went through a 2 hr incubation with secondary donkey anti-rabbit antibody AlexaFluor 488 (dilution 1:500; #A-21206, Thermo Fisher Scientific, Waltham, MA) at 4°C ...
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bioRxiv - Neuroscience 2019Quote: ... and chicken anti-mCherry (Novus, NBP2-25158, 1:1000) and secondary antibodies used were Alexa488 donkey anti-rabbit (Thermo Fisher, A21206, 1:500-1000) and Cy3 donkey anti-chicken (ImmunoJackson ...
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bioRxiv - Bioengineering 2020Quote: ... the samples were incubated with 2 µg/mL Alexa Fluor 488 labelled Goat anti-Rabbit IgG secondary antibody (ThermoFisher Scientific, stock solution, 2mg/mL) prepared by mixing the stock solution in 0.1% Triton/DPBS at 1/100 and 0.2 mM Hoechst 33342 (ThermoFisher Scientific ...
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bioRxiv - Plant Biology 2021Quote: ... the slides were incubated with secondary goat anti-rabbit or goat anti-rat antibodies conjugated with Alexa Fluor 488 or Alexa 555 (Thermo Fisher, diluted 1:100) in PBS containing 0.01 % acetylated BSA at 37 °C for 2h ...
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bioRxiv - Neuroscience 2020Quote: ... incubated with fluorescent-conjugated secondary antibodies [Alexa Fluor 488 Goat anti-Rabbit IgG (1:500 dilution; catalog # A-11034; Thermo Fisher Scientific, Tokyo, Japan)] diluted with the blocking solution for 2 hours at room temperature ...
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bioRxiv - Bioengineering 2020Quote: ... samples were washed three times with PBS and incubated with goat anti rabbit and goat anti mouse secondary antibodies for (Alexa Fluor 568) 1 h with (Thermo Fisher Scientific, 1:100). After washing ...
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bioRxiv - Systems Biology 2019Quote: ... the membrane was probed overnight at 4°C with the Rabbit anti-TAP polyclonal primary antibody (Thermo Fisher, 1:5000 dilution in blocking solution), which recognizes an epitope at the region between the TEV-cleavage site and His6 ...
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bioRxiv - Immunology 2020Quote: ... Cells were washed 4X in PBST and subsequently incubated with secondary antibodies (goat anti-rabbit IgG 488, Invitrogen, Carlsbad, CA; #A-11034, 1:4000; & goat anti-human 647, Invitrogen #A-21445, 1:4000) for 1 hour at room temperature ...
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bioRxiv - Neuroscience 2019Quote: ... Sections were then incubated at room temperature for 1 h with a goat anti-rabbit AlexaFluor 594-conjugated secondary antibody (Thermo Fisher Scientific, A-11012). Samples were mounted with Fluoromount-G (Fisher Scientific) ...
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bioRxiv - Microbiology 2019Quote: ... Goat anti-mouse Alexa-488 and goat anti-rabbit Alexa-568-conjugated used as secondary antibodies were obtained from Molecular Probes (Eugene, OR, USA) Horseradish peroxidase-conjugated goat anti-rabbit and anti-mouse antibodies were purchased from PerkinElmer Life Sciences (Boston ...
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bioRxiv - Neuroscience 2019Quote: ... followed by 2 hr incubation with secondary antibody (1:200 Alexa 555 anti-rabbit [Invitrogen]; 1:200 Alexa 488 anti-chicken [Invitrogen] made in PBS-T). Following three additional 10 min washes in PBS-T ...
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bioRxiv - Biophysics 2021Quote: ... The sections were rinsed in TBST and further incubated with the secondary antibody of goat anti-mouse FITC (1:500) or goat anti-rabbit Alexa Fluor-647(1:500) (Life Technologies, Thermo Scientific, USA) or goat anti-Guinea pig Alexa Fluor 555(1:500)for 2 h at room temperature in a humid chamber ...
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bioRxiv - Cancer Biology 2021Quote: ... Alexa Fluor488- and 594-conjugated anti-mouse and anti-rabbit secondary antibodies and AlexaFluor488-or Alexa594-conjugated peanut agglutinin (PNA) were purchased from Molecular Probes (Eugene, OR, USA) or from Invitrogen (Carlsbad ...
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bioRxiv - Cancer Biology 2021Quote: ... antibodies for 12 h at 4 °C followed by horseradish peroxidase (HRP)-conjugated goat anti-rabbit IgG (1/500; Invitrogen, Thermo Fisher Scientific, Inc.). After a PBS wash ...
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bioRxiv - Immunology 2020Quote: ... Membranes were developed with either Alexa Fluor® 660 anti-mouse IgG or Alexa Fluor® 680 anti-rabbit IgG antibodies (Thermo Fisher Scientific) and imaged using a LI-COR Odyssey Imager.
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bioRxiv - Microbiology 2021Quote: ... sc-376764) and anti-PbGAPDH rabbit polyclonal serum (1:100 dilution) or anti-mouse CD3 rat monoclonal antibody (Invitrogen, 14-0032-82; 1;100 dilution) for 16 h at 4°C ...
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bioRxiv - Genomics 2021Quote: ... overnight at 4° C followed by goat anti-rabbit Alexa fluor 594 secondary antibody (1:1000 in PBS with 1% (w/v) BSA) (Invitrogen, Carlsbad, CA, cat. A1102) incubation for 1 hr at room temperature ...
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bioRxiv - Neuroscience 2022Quote: ... slides were rinsed with 0.025% Triton X-100/1xTBS 2 × 5 min and incubated in goat anti-rabbit AlexaFlour488 secondary antibody (1:1000, Life Technologies, #A27034, Carlsbad, CA) at RT for at least 2 h followed by 4’,6-diamidino-2-phenylindol (DAPI ...
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bioRxiv - Neuroscience 2022Quote: ... sections were incubated at room temperature for 1 h with secondary antibodies Alexa Fluor 568 goat anti-mouse IgG and Alexa Fluor 488 goat anti-rabbit IgG (1:500; Invitrogen Life Technologies, Rockford, IL, USA), counterstained with Hoechst 33258 (1:1,500 ...
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bioRxiv - Cancer Biology 2022Quote: ... cells were incubated for 90 min at room temperature with secondary antibodies diluted in PBS/1% BSA: Alexa488 goat anti-rabbit (Life Technologies # A11070; 1/1200) or Alexa594 goat anti-mouse (Life Technologies # A11020 ...
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bioRxiv - Cancer Biology 2022Quote: Membranes were incubated for one hour in secondary antibody diluted in 5% NFDM TBST at a concentration of 80 ng/ml: Goat anti-rabbit or goat anti-mouse horseradish peroxidase (HRP)-conjugated secondary antibody (31460 and 31460 respectively, Thermo Fisher Scientific, Waltham, MA). Clarity Western ECL (Biorad ...
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bioRxiv - Cancer Biology 2022Quote: ... Secondary antibody incubation was conducted using Alexa Fluor 488 and goat anti-rabbit IgG (H+L) (Thermo Fisher Scientific, Waltham, MA, USA; A-11008) at a 1:1,000 dilution ...
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bioRxiv - Bioengineering 2022Quote: ... Fluorophore-conjugated secondary antibody staining (anti-rabbit AlexaFluor® 488, anti-mouse AlexaFluor® 594, anti-mouse AlexaFluor® 647; Molecular Probes; 1:800) was performed at room temperature for 1 h in the dark ...
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bioRxiv - Cell Biology 2022Quote: ... Slides were washed three times with PBST before incubation with Alexa Fluor® 488 donkey anti-rabbit IgG secondary antibody (Invitrogen, Carlsbad, CA, USA), for 1 hr at RT ...
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bioRxiv - Cell Biology 2022Quote: ... IgG and anti-streptavidin treated PS DAAM-particles were stained with donkey anti-rabbit-Alexa Fluor-647 antibodies (Invitrogen, A31573, 1:1000 in PBS) for 30 min ...
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bioRxiv - Developmental Biology 2022Quote: ... The next day the membranes were incubated with HRP-conjugated secondary antibodies Goat anti-Rabbit IgG (H+L) (1:5000; Thermo Fisher Scientific, Cat# 31466) or Goat anti-Mouse IgG (H+L ...
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bioRxiv - Cell Biology 2022Quote: ... primary antibody at a 1:500 dilution in 1% BSA for 90 min at room temperature or 24 hr at 4°C and was followed by incubation with Alexa Fluor 488 goat anti-rabbit secondary antibody (Invitrogen A11008; 4 μg/ml) in 0.2% BSA for 60 min at room temperature ...
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bioRxiv - Microbiology 2022Quote: ... and Alexa Fluor™ 488 Goat anti-Rabbit IgG (H+L) Highly Cross-Adsorbed Secondary Antibody (1:500; Thermo Fisher Scientific cat. No. A11034). Neurons were simultaneously stained with 4=,6-diamidino-2-phenylindole (DAPI ...
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bioRxiv - Plant Biology 2022Quote: ... They were then treated with secondary antibody solution (1 : 200 of Alexa Fluor 488 goat anti-rabbit IgG; Molecular Probes, in the blocking buffer) for 2 hours at 37 °C and washed three times ...
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bioRxiv - Molecular Biology 2023Quote: ... The following secondary antibodies were used for immunofluorescence: Goat α Mouse or Goat α Rabbit labelled with either Alexa 488 or Alexa 647 (Invitrogen; 1:1,000).
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bioRxiv - Microbiology 2022Quote: ... and washed 3 times with PBS before incubation for 1 h with goat anti-rabbit IgG secondary antibody Alexa fluor 594 (Thermo Fisher Scientific, Waltham, MA), and 100 ng/mL DAPI (Sigma-Aldrich) ...
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bioRxiv - Neuroscience 2023Quote: ... 2 % GS and the secondary antibodies (anti-mouse IgG, 1:500, AF488, #A10680, Thermo Fisher Scientific, USA; anti-rabbit IgG, 1:1000, AF546, #A11010, Thermo Fisher Scientific, USA) was added and incubated for 2 h at 4 °C ...
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bioRxiv - Neuroscience 2023Quote: ... Cells were centrifuged and washed 2 times in cold flow buffer and resuspended in donkey-rabbit AF647 secondary antibody (Life Technologies, A31573, 1:1000) and DAPI (Biovision ...
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bioRxiv - Neuroscience 2022Quote: ... the sections were incubated for 1 hour in goat anti-rabbit or goat anti-mouse AlexaFluor488/546 secondary antibodies (Invitrogen Life Technologies, Carlsbad, CA). Sections were mounted and examined using an LSM 710 confocal microscope (Zeiss ...
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bioRxiv - Neuroscience 2024Quote: ... Slice were then incubated overnight at 4°C in blocking solution containing a rabbit anti-Parvalbumin (PV) antibody (1:400, cat # PA1933, Thermo Fisher Scientific, Waltham, MA). Slices were then washed and incubated blocking solution containing a secondary antibody donkey anti-rabbit Alexa568 diluted to 1:1000 (Abcam ...
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bioRxiv - Cell Biology 2024Quote: ... Sections were washed three times with 1X PBS followed by incubation with IgG-conjugated secondary antibodies (goat anti-mouse IgG overnight and Alexa Fluor™ 488 goat anti-rabbit IgG for 4 h (both from ThermoFisher Scientific, 1:1000). Sections were washed three times as above and followed by 1 h incubation with Alexa Fluor™594 Tyramide SuperBoost for ERG staining ...
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bioRxiv - Neuroscience 2024Quote: ... This was followed by a 1 h room temperature incubation with a horseradish peroxidase-conjugated rabbit anti-goat secondary antibody (1:3000; Thermo Fisher Scientific, Waltham, MA). The membranes were developed using Clarity Western ECL (Bio-Rad Laboratories ...