Labshake search
Citations for Thermo Fisher :
7451 - 7500 of 10000+ citations for Biotin Z Antibody Internalization Kit rabbit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Cell Biology 2021Quote: ... were conjugated with Alexa Fluor 647 dye using the Apex Antibody Labelling Kit (Invitrogen, Carlsbad, CA, USA) according to the manufacturer’s protocol ...
-
bioRxiv - Immunology 2021Quote: ... performed intracellular staining with antibodies diluted in eBioscience Foxp3/Transcription Factor kit perm wash (Thermo Fisher Scientific), fixed cells in freshly prepared 2% paraformaldehyde (Electron Microscopy Sciences ...
-
bioRxiv - Microbiology 2022Quote: ... and the antibodies were purified using NAb protein A plus spin kit (Thermo Fisher Scientific, Cat# 89948) according to the manufacturer’s protocol.
-
bioRxiv - Plant Biology 2022Quote: ... Dynabeads coupled with anti-O1 antibody 11759 and 117560) were prepared according to Dynabeads kit (Thermo Fisher) and added to the supernatant ...
-
bioRxiv - Molecular Biology 2020Quote: ... Antibody binding was detected using enhanced chemiluminescence HRP substrate detection kit for standard (Thermo Fisher Scientific, #32106), medium (Thermo Fisher Scientific ...
-
bioRxiv - Immunology 2022Quote: ... IgA antibodies in the BALF were measured using a mouse Total IgA ELISA kit (Thermo Fisher Scientific) according to manufacturer’s instructions with the exception of the coating antigen ...
-
bioRxiv - Neuroscience 2019Quote: ... Affinity purification of the protein-antibody complex was performed using the AminoLink Immobilization Kit (Thermo Scientific #44890). Approximately 6 mg of soluble dNOS exon 16 protein in 2M guanidine in 1X PBS was bound to the agarose beads in the column as antigen ...
-
bioRxiv - Cancer Biology 2020Quote: Durvalumab antibody was covalently immobilized to 4% beaded agarose using AminoLink Plus Immobilization Kit (Thermo Scientific # 44894) according to manufacturer’s instructions (4 mg antibody per 1 ml resin) ...
-
bioRxiv - Cancer Biology 2019Quote: ... Monoclonal β-Actin (AM4302) antibody and EpiJET Bisulfite Conversion Kit were purchased from Thermo Scientific (PA, USA). Monoclonal CSTA antibody (ab10442 ...
-
bioRxiv - Physiology 2021Quote: ... and HRP-linked secondary antibody (ThemoFisher) and visualized with a chemiluminesce detection kit (SuperSignal West Femto; ThermoFisher) and a gel imaging system (ChemiDoc XRS ...
-
bioRxiv - Cell Biology 2022Quote: ... Labelling of EdU was performed after secondary antibody incubation using Click-It EdU Kit (ThermoFisher Scientific #c10337). Nuclear counterstain was performed by 10 sec incubation with Hoechst (Sigma-Aldrich #14533 ...
-
bioRxiv - Cell Biology 2022Quote: ... Labelling of EdU was performed after secondary antibody incubation using Click-It EdU Kit (ThermoFisher Scientific #c10337). Nuclear counterstain was performed by 10 sec incubation with Hoechst (Sigma-Aldrich #14533 ...
-
bioRxiv - Molecular Biology 2022Quote: ... Antibodies were produced by transfecting the IgG1 expression constructs using the ExpiFectamine™ 293 Transfection Kit (ThermoFisher) in Expi293F (ThermoFisher ...
-
bioRxiv - Immunology 2024Quote: ... Single color controls for panel compensation were made using AbC Total Antibody Compensation Bead kit (ThermoFisher Scientific), by staining each control with one of the panel antibodies and using these controls to compensate the flow cytometer experiment prior to running the samples ...
-
bioRxiv - Microbiology 2023Quote: ... IgA antibody levels were determined by using the mouse IgA ELISA kit (Thermofisher, Cat#88-50450-88). The endpoint titers of mouse sera were calculated using the interpolation in GraphPad Prism 9.0 with a cutoff of 2.5 times the Mean-negative.
-
bioRxiv - Cancer Biology 2023Quote: ... Gal-9 and CQ (from the LC3B Antibody Kit for Autophagy, L10382, Invitrogen™, Carlsbad, CA, USA), 5×104 cells (either cell lines or primary patient-derived material ...
-
bioRxiv - Immunology 2023Quote: ... The slides were then treated with poly-HRP conjugated secondary antibody from Tyramide SuperBoost™ kit (Invitrogen) and the developed with tyramide working solution with Alexa Fluor 594 from Tyramide SuperBoost™ kit (Invitrogen ...
-
bioRxiv - Biochemistry 2023Quote: ... antibody-labeled cells were then mixed with magnetic beads from Dynabeads® FlowComp™ Flexi kit (Invitrogen), at a ratio of 37.5 μL of washed beads per 50 μL of cell suspension ...
-
bioRxiv - Microbiology 2023Quote: ... and the antibodies were purified using NAb protein A plus spin kit (Thermo Fisher Scientific, Cat# 89948) according to the manufacturer’s protocol.
-
bioRxiv - Cancer Biology 2024Quote: ... In-house primary antibody fluorescent conjugates were generated using the Alexa Fluor 647 labelling kit (Invitrogen, A20186) or the Alexa Fluor 555 labelling kit (Invitrogen ...
-
bioRxiv - Biophysics 2020Quote: Oocytes expressing Wild-type Cx30 or Cx30G11R were washed out with cold PBS and then incubated with PBS containing 0.5 mg/mL EZ-LinkTM NHS-Biotin (Thermo Fisher Scientific) for 10 minutes at 4°C ...
-
bioRxiv - Immunology 2021Quote: ... and wild type or IL-7rα-/- (both CD45.2+) were depleted of lineage-positive cells by magnetic separation using Biotin Binder Dynabeads (Thermo Fisher Scientific) after staining with biotinylated antibodies against CD3 ...
-
bioRxiv - Genetics 2021Quote: ... and the amount of Nb127D01-biotin bound to the plate was quantified through the addition of streptavidin-HRP (Thermo Fisher, N100), washing ...
-
bioRxiv - Genomics 2020Quote: ... The DNA overhangs were filled with biotinylated nucleotides by addition of 100 μL pre-mix (25 μL 0.4 mM Biotin-dATP (Thermo Fisher, 19524016), 25 μL 0.4 mM Biotin-dCTP (Thermo Fisher ...
-
bioRxiv - Molecular Biology 2021Quote: ... biotin labelled Hi-C 3.0 DNAs were purified by 20 μl Dynabeads® MyOne™ Streptavidin C1 beads (Thermo Fisher 65002). The biotinylated-DNA on C1 beads was used to perform on-beads library making with NEBNext® Ultra™ II DNA Library Prep Kit for Illumina (NEB ...
-
bioRxiv - Molecular Biology 2020Quote: ... 400 ng of total RNA was labeled with biotin and hybridized to the miRNA 4.0 Array (ThermoFisher Scientific, Waltham, MA, #902412) for 18 hours at 48°C using an Affymetrix® 450 Hybridization Oven (Affymetrix ...
-
bioRxiv - Molecular Biology 2021Quote: ... The 4sU-labelled RNA was thiol-specific biotinylated by addition of 200 μg EZ-link HPDP-biotin (ThermoFisher Scientific, Cat# 21341), biotinylation buffer (10 mM Hepes-KOH pH 7.5 and 1 mM EDTA ...
-
bioRxiv - Molecular Biology 2020Quote: ... Restriction fragments were biotinylated by supplementing the reaction with 60 μl of fill-in mix (0.25 mM biotin-14-dATP (Life Technologies, 19524016), 0.25 mM dCTP ...
-
bioRxiv - Immunology 2022Quote: ... if required by the assay and excessive biotin was removed by size exclusion chromatography using Zeba-Spin desalting columns (7kDa cutoff, Thermo Fisher).
-
bioRxiv - Cell Biology 2019Quote: ... Cells were harvested before the cultures reached OD600 = 1 and were labeled with 8 mg/ml EZ-Link Sulfo-NHS-LC-biotin (ThermoFisher Scientific) for 30 minutes at 4°C ...
-
bioRxiv - Immunology 2019Quote: AF647 and biotin-conjugated anti-mouse FCRL6 (1C3 and 3C1) were generated and labeled by our laboratory (Invitrogen and Thermo Scientific). Anti-VH11 (3H7 (Rowley et al. ...
-
bioRxiv - Immunology 2019Quote: AF647 and biotin-conjugated anti-mouse FCRL6 (1C3 and 3C1) were generated and labeled by our laboratory (Invitrogen and Thermo Scientific). Anti-VH11 (3H7 (Rowley et al. ...
-
bioRxiv - Biochemistry 2019Quote: CMG2-GST (AlexaFluor 488 or biotin) and PA-E733C (AlexaFluor 546) were conjugated on a single reactive cysteine with maleimide-labels (Thermo Fisher) after GST was cleaved by using thrombin ...
-
bioRxiv - Biophysics 2021Quote: ... and biotin-PEG-SVA at a ratio of 99:1 (w/w) (Laysan Bio) in 0.1 M sodium bicarbonate (Thermo Fisher Scientific) for 3 h.10 Excess PEG was rinsed with water ...
-
bioRxiv - Bioengineering 2020Quote: A cell line derived from Expi293 cells expressing an ER-localized biotin ligase (BirA) gene was grown in Expi293 media (Thermofisher Scientific) supplemented with 100 µM biotin (GoldBio ...
-
bioRxiv - Biochemistry 2020Quote: Biotinylated heparin was prepared by mixing 2 mg of heparin and 2 mg of amine– PEG3–Biotin (Thermo Scientific, Waltham, MA) with 10 mg of sodium cyanoborohydride (NaCNBH3 ...
-
bioRxiv - Neuroscience 2021Quote: ... Washed cells were incubated with PBS2+ containing 2 mg mL−1 Pierce Premium Grade Sulfo-NHS-SS-Biotin (PG82077; Thermo Scientific) for 30 min on ice ...
-
bioRxiv - Neuroscience 2021Quote: ... α-Syn overexpressing neuronal cultures and non-transduced neuronal cultures were washed three times with cold PBS and incubated with sulfo-NHS-biotin (1.5 mg/ml; ThermoFisher Pierce, 21331) for 30 minutes at 4 °C with rocking ...
-
bioRxiv - Cancer Biology 2020Quote: ... which then were cross-linked to polymerizing PAA gels premixes by their biotin tags to streptavidin-conjugated polyacrylamide (Streptavidin-acrylamide, Thermo Fisher). For that 7-10 μL of PAA premix of desired G’ was polymerized in the “sandwich” fashion between an “intermediate” patterned surface and glass-bottom 35 mm Petri dishes (MatTek Corp. ...
-
bioRxiv - Microbiology 2020Quote: ... Unreacted nanobody and sortase was removed with Ni-NTA resin and excess GGGK-biotin was removed using Zeba spin desalting columns (0.5 mL, 7k MWCO, Thermo Fisher Scientific).
-
bioRxiv - Neuroscience 2021Quote: ... N2a cells were washed twice with cold PBS and incubated for 30 min with 0.5 mg/mL EZ-Link Sulfo-NHS-SS-Biotin (Thermo Fisher Scientific) in PBS shaking at 4°C ...
-
bioRxiv - Neuroscience 2020Quote: ... The neurons were incubated with 1.5 mg/mL EZ-Link™ Sulfo-NHS-S-S-Biotin (#21331; Thermo Fisher Scientific, USA) in PBS for 30 minutes at 37°C ...
-
bioRxiv - Genomics 2022Quote: ... resuspended in ice cold PBS+BE (1 mg/mL BSA + 2 mM EDTA) and incubated for an hour at +4°C with Dynabeads Biotin Binder (Thermo Fisher). Cells were repeatedly washed with ice-cold sterile PBS while on magnet ...
-
bioRxiv - Biophysics 2022Quote: The full-length CcdA and CcdA peptides of different lengths (Table S1) were biotinylated with EZ-linkTM NHS-Biotin (ThermoFisher Scientific) as per the manufacturer’s protocol ...
-
bioRxiv - Biochemistry 2022Quote: ... The labelled lysate was then clicked on with biotin-alkyne using copper catalyzed azide-alkyne click chemistry reaction protocol according to manufacturer’s instruction (ThermoFisher Scientific C33372). The biotinylation was detected using IRDye 800CW Streptavidin dye and imaged (Odyssey Image System LICOR).
-
bioRxiv - Neuroscience 2021Quote: ... Two slices were incubated in in chilled ACSF containing 1 mg/ml EZ-link Sulfo-NHS-SS-Biotin (Thermo Fisher Scientific) for 45 minutes ...
-
bioRxiv - Microbiology 2021Quote: ... recombinant SARS-CoV-2 Spike RBD protein (RBD, His Tag) was conjugated with EZ-LinkTM Sulfo-NHS-Biotin (1:3 molar ratio; Thermo Fisher) in Dulbecco’s PBS at room temperature for 30 min ...
-
bioRxiv - Neuroscience 2020Quote: ... Cells were then washed twice with ice cold PBS (4° C) and subsequently transferred to cold PBS containing Ez-link Sulfo-NHS-LC-biotin (1 mg/ml; ThermoFisher Scientific) for 45 min ...
-
bioRxiv - Neuroscience 2021Quote: ... acute hippocampal slices were labeled for 30 min at 4 °C with 1 mg/ml sulfo-NHS-SS biotin (ThermoFisher Scientific). Membranes were prepared and the biotinylated proteins were precipitated with streptavidin agarose resin (ThermoFisher Scientific ...
-
bioRxiv - Neuroscience 2021Quote: ... The collected supernatant was then subjected to dialysis to remove excess free biotin using a Slide-A-Lyzer MINI dialysis device (Thermo Scientific). Proteins were precipitated by adding 9 parts methanol to 1 part lysate ...