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Citations for Thermo Fisher :
7501 - 7550 of 10000+ citations for Biotin Z Antibody Internalization Kit rabbit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2021Quote: ... Typhimurium was grown in BHI broth until OD600:1 and then biotinylated with EZ-link NHS-Biotin reagent (Thermo Fisher Scientific). After washing ...
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bioRxiv - Microbiology 2020Quote: ... recombinant SARS-CoV-2 spike protein was conjugated with EZ-Link™ Sulfo-NHS-Biotin (1:3 molar ratio; Thermo Fisher) in Dulbecco’s PBS at room temperature for 30 min ...
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bioRxiv - Microbiology 2021Quote: ... cells were washed once with PBS (pH 8) and incubated with 1 mg/ml of EZ-link Sulfo-NHS-biotin (Thermo Fisher) in PBS (pH 8 ...
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bioRxiv - Cell Biology 2022Quote: ... Five hundred ng of high-quality RNA was hybridized to biotin and subsequently onto Affymetrix Mouse Genome 430 2.0 GeneChips according to the manufacturer’s protocols (900497, Applied Biosystems, Waltham, MA). Thereafter ...
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bioRxiv - Bioengineering 2019Quote: ... the GVs were biotinylated by incubating them for 1 hour with a 10,000-fold molar excess of sulfo-NHS-biotin (Thermo Fisher Scientific). Then ...
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bioRxiv - Molecular Biology 2019Quote: ... the slides were washed in 2X SSC and biotin was detected with Streptavidin AF 488 (ThermoFisher S-32354; Lot# 1571714, 1977355). Slides were mounted and counterstained in Vector shield mounting medium with DAPI (Vector laboratories ...
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Gatekeeper helix activates Golgi SM protein Sly1 and directly mediates close-range vesicle tetheringbioRxiv - Cell Biology 2020Quote: ... content mixing FRET reporters were then added (500 µL of 4 mg/mL solution of R-phycoerythrin-biotin conjugate, or 296 µL of 2mg/mL Alexa-Streptavidin; both reagents from Thermo/Molecular Probes). SNARE stocks in SNARE elution buffer with β-OG were then added to a final molar ratio of 1:600 (each Q-SNARE ...
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bioRxiv - Immunology 2020Quote: ... Cells were stained with human MR1-5-OP-RU tetramer for 40 minutes at RT in the dark prior to the addition of anti-bovine CD8-biotin (Clone ILA51) and Near-Infrared Fixable LIVE/DEAD stain (Invitrogen or Molecular Probes) for 20 minutes at 4 °C ...
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bioRxiv - Cell Biology 2019Quote: ... The cell surface was biotinylated by incubating cells for 10 min with 1 mg of EZ-Link-Sulfo-NHS-LC-biotin (Thermo Scientific) in Sorensen-Sorbitol buffer at pH 8 on ice ...
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bioRxiv - Bioengineering 2019Quote: GV clusters were prepared by first biotinylating purified GVs with 105 molar excess of EZ-Link Sulfo-NHS-LC-biotin (Thermo Scientific) in PBS for 4 hours ...
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bioRxiv - Cell Biology 2019Quote: ... The cell surface was then biotinylated by incubating cells for 3 min with 30 mg of EZ-Link-Sulfo-NHS-LC-Biotin (Thermo Scientific) in Sorensen-Sorbitol buffer at pH 8 on ice ...
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bioRxiv - Neuroscience 2020Quote: Rodent astrocytes were plated in 6 well plates at a density of 500,000 cells/well and live cells were treated with EZ-LinkTMNHS-Biotin reagent (Thermo Scientific, 20217). Cells were then homogenized and pelleted ...
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bioRxiv - Evolutionary Biology 2021Quote: ... cells were washed twice with ice-cold PBS2+ (with Ca2+ and Mg2+) and incubated with 0.5 mg/ml EZ-Link Sulfo NHS-Biotin (Thermo Fisher Scientific) for 30 min on ice ...
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bioRxiv - Biochemistry 2021Quote: Protein content in cell culture lysates and eluates from biotin/streptavidin or acyl-RAC mediated protein purification was determined by the BCA assay (Pierce, Thermofisher Scientific) and mixed with 4x reducing Laemmli buffer to yield the indicated protein amounts in 1x Laemmli buffer ...
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bioRxiv - Physiology 2020Quote: ... TsA-201 cells transiently expressing Nav1.5 constructs were supplied with 0.5 mg/mL EZ-link™ Sulfo-NHS-SS-Biotin (Thermo Fisher Scientific) in PBS for 15 min at 4°C ...
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bioRxiv - Cancer Biology 2021Quote: ... and incubated in click reaction buffer (10 mM sodium-l-ascorbate, 20 μM biotin azide [Life Technologies], and 2 mM CuSO4) for 2h at room temperature ...
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bioRxiv - Developmental Biology 2020Quote: ... Biotinylation of cell surface proteins using a non-cell permeable and thiol-cleavable probe was initiated by incubating cells with 0.4 mM EZ-Link Sulfo-NHS-SS-Biotin (Thermo Fisher Scientific) in DPBS+ buffer for 30 minutes ...
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bioRxiv - Cell Biology 2020Quote: ... and either PFA-fixed at indicated times for immunofluorescence imaging or subjected to incubation on ice with Sulfo-NHS-SS-Biotin (Thermo Scientific) to biotinylate all surface proteins ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: Cell surface proteins were labeled by incubating cells with 1.6 mM Sulfo-NHS-SS-Biotin (Thermo Fisher Scientific, Waltham, MA USA) or 1.6 mM Biotin-dPEG3-Maleimide (MilliporeSigma ...
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bioRxiv - Bioengineering 2021Quote: ... biotin-streptavidin binding was used to functionalize magnetic particles (Dynabeads MyOne™ Streptavidin C1, Cat. #65001, Life Technologies, Carlsbad, CA, USA) with antibodies for CTXII (cat ...
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bioRxiv - Developmental Biology 2022Quote: ... Biotin pulldown was performed by adding an equal volume of Hi-C sample with Dynabeads MyOne Streptavidin T1 beads (Invitrogen, 65602) and incubated by 15 minutes with rotation ...
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bioRxiv - Biochemistry 2022Quote: Purified protein at a concentration of 1 mg/ml was chemically biotinylated using EZ link NHS-PEG4-Biotin (Thermo Scientific, A39259) at a 10-30 times molar excess ...
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bioRxiv - Biophysics 2022Quote: 96 well glass plates (Matek, PBK96G-1.5-5-F) were incubated with 80 μL of 100 ug/ml BSA-Biotin (Thermo Fisher, 29130) in PBS for 2 hours at room temperature ...
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bioRxiv - Plant Biology 2022Quote: ... The electrophoretic migration of biotin-labeled probes was resolved on 6% native polyacrylamide gels and detected using an enhanced chemiluminescence substrate (Thermo Scientific).
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bioRxiv - Microbiology 2022Quote: ... 1mM DTT, 300 mM KCl, 20µM biotin-11-ATP, -UTP, -GTP, -CTP, 0.4 U/ml RNase inhibitor [RNaseOUT ThermoFisher] and 1% Sarkosyl) was added to thawed nuclei ...
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bioRxiv - Molecular Biology 2022Quote: ... targeting receptor-binding domains of SARS-CoV-2 spike glycoprotein which were discovered in Song et al.22 Biotin-modified aptamers and ACE2 were bound to streptavidin-coated polystyrene plates (ThermoFisher #PI15500). The aptamers and ACE2 captured the recombinant spike protein ...
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bioRxiv - Biophysics 2022Quote: ... 20–25 oocytes per sample were washed x6 in ND96 and labeled with 0.5 mg/ml EZ-Link™ Sulfo-NHS-SS-Biotin (Thermo Fisher) for 30 min at room temperature ...
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bioRxiv - Pathology 2024Quote: ... with a biotin conjugate was used (1:200) followed by AB reagent or streptavidin conjugated with AlexaFlour 488/594 fluorophore (ThermoFisher Scientific). For IF detection ...
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bioRxiv - Microbiology 2024Quote: ... A 1 mL suspension of the bacterial cells was mixed with EZ-Link Sulfo-NHS-LC-Biotin (Thermo Fisher Scientific, #A39257) dissolved in 100 µl of sterile MilliQ water ...
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bioRxiv - Developmental Biology 2024Quote: Transfected COS7 cells on a 100-mm dish were biotinylated by incubation in 1 mg/ml NHS-SS-Biotin (21331, Thermo Scientific), diluted in 1× PBS containing 1 mM MgCl2 and 0.1 mM CaCl2 (PBS–MC ...
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bioRxiv - Molecular Biology 2024Quote: ... The resulting chains were then incubated overnight at room temperature with 10x molar excess of EZ-Link Maleimid-PEG2-Biotin (Thermo Scientific). The next day ...
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bioRxiv - Neuroscience 2024Quote: ... The cell culture medium was removed and cells were suspended with 1 mL ice-cold PBS and then treated with 1 mL sulfo-N-hydroxysuccinimide-SS-biotin (Thermo Scientific) (1 mg/mL in PBS ...
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bioRxiv - Cell Biology 2024Quote: ... human TIM4-Fc protein (FUJIFILM Wako Pure Chemical Corporation) was biotinylated with EZ-Link™ Maleimide-PEG11-Biotin (Thermo Fisher Scientific) and conjugated to Dynabeads™ MyOne™ Streptavidin C1 magnetic beads (65001 ...
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bioRxiv - Immunology 2023Quote: ... Plates were washed 3 times with PBS-T and incubated with 1.6 μg/ml biotin-conjugated goat anti-mouse IgM (Thermo Fisher Scientific) or 4 μg/ml biotin-conjugated goat anti-mouse IgG1 (Thermo Fisher Scientific ...
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bioRxiv - Cell Biology 2024Quote: Live cells were washed three times with ice-cold PBS complete (including Ca++ and Mg++) and incubated for 30 minutes on ice in PBS complete with 0,8 mM Sulfo-NHS-SS-Biotin (Thermo Fisher Scientific). Cells were washed once with PBS complete ...
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bioRxiv - Genomics 2024Quote: ... Biotinylation was performed in labelling buffer (10 mM HEPES–KOH pH 7.5 and 1 mM EDTA) and 0.2 mg/mL Biotin-HPDP (ThermoFisher Scientific, Cat# 21341) for 3 h at room temperature at 24°C in the dark and with gentle agitation ...
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bioRxiv - Biochemistry 2024Quote: ... 0.15 pmol DNA of 3 kb PCR substrates with a single biotin (GC227 and GC228) were coupled with 5 μl of Dynabeads M-280 streptavidin (Invitrogen 11205D) in 10 μl of binding buffer (5 mM Tris-HCl ...
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bioRxiv - Cancer Biology 2024Quote: ... Telomeric DNA was identified by hybridization with a Biotin-OO-(CCCTAA)4 PNA probe (TelC, Biosynthesis) followed by incubation with Alexa Fluor 350-conjugated NeutrAvidin (Molecular Probes), followed by two rounds of incubation first with a biotinylated anti-avidin antibody (Vector ...
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bioRxiv - Neuroscience 2024Quote: ... the protein GFP selector beads were washed with pH 6.2 LB twice and treated with 1.6 µM EZ-LinkTM BMCC-Biotin (ThermoFisher Scientific, Waltham, MA) in pH 6.2 LB for 45 min at 4°C ...
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bioRxiv - Microbiology 2023Quote: ... 100 μl of His-GLPR at 25 μM was incubated with 20x molar excess of EZ-Link NHS-PEG4-Biotin (Thermo Scientific) following supplier instructions ...
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bioRxiv - Plant Biology 2022Quote: ... Prior to streptavidin affinity pulldown free biotin was removed from protein samples using a Zeba™ Spin Desalting column (#89891, Thermofisher). To determine their protein concentration ...
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bioRxiv - Microbiology 2023Quote: ... All probes were single-stranded DNA oligonucleotides synthesised with biotin at the 3’ end (Integrated DNA Technologies) and were detected using streptavidin-Alexa Fluor 488 conjugate (Life Technologies). Cells were then immuno-stained for viral proteins following the immunofluorescence staining protocol ...
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bioRxiv - Neuroscience 2023Quote: ... Cell surface proteins were biotinylated using the EZ-Link™ Sulfo-NHS-SS-Biotin reagent (Cat No. 21331, Thermo Fisher Scientific). Cells were treated in three experimental conditions (VLIUS ...
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bioRxiv - Microbiology 2023Quote: ... anti-mouse IL-1β biotin conjugate (13-7112-81), and anti-mouse IL-6 (MP5-20F3, 14-7061-85) were purchased from Thermo Fisher Scientific ...
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bioRxiv - Molecular Biology 2023Quote: ... cells were washed three times with ice cold PBS-CM (PBS-CaCl2/MgCl2) and incubated with 0.5 mg/ml Ez-Link Sulfo-NHS-LC-Biotin solution (Thermo Scientific, 21335) diluted in PBS-CM on the shaking for 30 min at 4C ...
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bioRxiv - Cancer Biology 2023Quote: ... Nascent RNAs labeled with biotin were isolated from total RNA using streptavidin-coated beads (Dynabeads MyOne Streptavidin C1, Thermo Fisher Scientific). TruSeq small RNA adaptor was ligated to 3’ ends of nascent RNAs and transcripts purified with a second round of streptavidin bead purification ...
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bioRxiv - Immunology 2023Quote: ... Excess of biotin was removed by size exclusion chromatography using ZebaTM Spin Desalting Columns 7K MWCO 0.5 ml (Thermo Fisher Scientific) according to manufacturer’s protocol ...
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bioRxiv - Immunology 2023Quote: ... 7×108 CEA10 (for AF633-labeled) or CEA10-RFP (for FLARE) conidia were rotated in 10 μg/ml Sulfo-NHS-LC-Biotin (Thermo Scientific) in 1 ml of 50 mM carbonate buffer (pH 8.3 ...
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bioRxiv - Immunology 2023Quote: ... Beads were washed using the PTFE filters and resuspended in 1x PBS or further biotinylated with 6 mg/ml EZ-link™ Sulfo-NHS-SS-biotin (#21331; ThermoFisher). Streptavidin conjugated to either AlexaFluor-488 or -568 was then added for 1h RT incubation ...
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bioRxiv - Biochemistry 2023Quote: Biotin-conjugated cholera toxin B subunit (CTB) used for the CRISPR screen was purchased from Life Technologies (catalog no. C-34779). Azide-free cholera holotoxin (CT ...