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Citations for Thermo Fisher :
7551 - 7600 of 10000+ citations for Biotin Z Antibody Internalization Kit rabbit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2023Quote: ... 7×108 CEA10 (for AF633-labeled) or CEA10-RFP (for FLARE) conidia were rotated in 10 μg/ml Sulfo-NHS-LC-Biotin (Thermo Scientific) in 1 ml of 50 mM carbonate buffer (pH 8.3 ...
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bioRxiv - Neuroscience 2023Quote: The incorporation of biotin moieties for downstream detection of nanobody constructs was achieved using a 5-fold molar excess of EZ-link-NHS-PEG4-biotin (#21330, Thermo Fisher) with nanobody constructs in 50mM sodium carbonate buffer ...
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bioRxiv - Cancer Biology 2023Quote: ... and incubated in click reaction buffer (10 mM sodium-l-ascorbate, 20 µM biotin azide [Life Technologies], and 2 mM CuSO4) at RT for 2 hours on a rotator ...
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bioRxiv - Developmental Biology 2024Quote: Transfected COS7 cells on a 100-mm dish were biotinylated by incubation in 1 mg/ml NHS-SS-Biotin (21331, Thermo Scientific), diluted in 1× PBS containing 1 mM MgCl2 and 0.1 mM CaCl2 (PBS–MC ...
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bioRxiv - Immunology 2023Quote: ... Plates were washed 3 times with PBS-T and incubated with 1.6 μg/ml biotin-conjugated goat anti-mouse IgM (Thermo Fisher Scientific) or 4 μg/ml biotin-conjugated goat anti-mouse IgG1 (Thermo Fisher Scientific ...
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bioRxiv - Biophysics 2023Quote: ... and the adapters were then PCR amplified from this plasmid with 22% of dATP replaced by biotin-14-dATP (Invitrogen 19524016) for the biotin adapter and 22% of dTTP replaced by digoxigenin-11-dUTP (MilliporeSigma 11093088910 ...
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bioRxiv - Microbiology 2024Quote: ... A 1 mL suspension of the bacterial cells was mixed with EZ-Link Sulfo-NHS-LC-Biotin (Thermo Fisher Scientific, #A39257) dissolved in 100 µl of sterile MilliQ water ...
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bioRxiv - Cell Biology 2024Quote: ... 2.5×10^5 cells per well were cultured overnight in a 6-well plate and surface labeled with NHS-LC biotin (Thermofisher#21335) at a concentration of 0.2 mg/ml in PBS for 20 minutes on ice ...
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bioRxiv - Immunology 2024Quote: ... Biotin-coupled DNA-protein complex was pulled down by incubating whole cell lysates with high-capacity streptavidin agarose beads (Thermo Fisher) according to the manufacturer’s instructions ...
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bioRxiv - Genetics 2024Quote: ... Biotin probes hybridized with GFP mRNA were then captured by adding 400 uL of pre-washed Sera Mag Beads (Fisher Scientific) eluted in 500 uL of 20X SSX by agitation at room temperature for 15 minutes ...
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bioRxiv - Biophysics 2024Quote: ... MSP1E3D1 was expressed and purified as previously described (79) and biotinylated with EZ-Link NHS-polyethylene glycol 4 (PEG4)-Biotin (Thermo Fisher) per manufacturer instructions ...
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bioRxiv - Neuroscience 2024Quote: ... astrocytes were switched to high protein conditioning media without biotin (components 50% Neurobasal Thermo 12348017, 50% DMEM LifeTech 11960044, supplemented with Penicillin-Streptomycin [Thermo Fisher Scientific 15140-122] ...
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bioRxiv - Biophysics 2024Quote: ... Streptavidin was bound to the biotin-terminated PEGs by addition of 20 µL of 10 nM streptavidin (Invitrogen, Catalog Number S888) and free streptavidin was removed by rinsing the well 4 times with 70 µL Standard Reaction Buffer ...
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bioRxiv - Animal Behavior and Cognition 2024Quote: ... slices were placed on ice to stop endocytosis and were washed with ice-cold aCSF containing 0.9 mg/ml sulfo-NHS-SS-biotin (Thermo Fisher scientific) for 15min ...
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bioRxiv - Cell Biology 2024Quote: ... Cell were washed once in 0.5% BSA/PBS and once in PBS before performing the Click-iT reaction with Biotin-Azide (Thermo Fisher Scientific) at RT for 2 h ...
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bioRxiv - Biochemistry 2020Quote: ... Secondary antibody probing was for 1 hour in the dark with the following antibodies at 1:1000 in blocking buffer: goat anti-rabbit Alexa Fluor 594 (Life Technologies, Carlsbad CA, A11037) and goat anti-mouse Alexa Fluor 488 (Life Technologies ...
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bioRxiv - Cell Biology 2020Quote: ... The secondary antibodies used were Alexa 488 goat anti-rabbit IgG and Alexa 568 goat anti-guinea pig IgG (Thermo Fisher Diagnostics, Tokyo, Japan). The lack of cross-reactivity of the antibodies was also confirmed (data not shown) ...
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bioRxiv - Cell Biology 2019Quote: ... membranes were washed with 0.1% Tween in tris-buffered saline before incubation with appropriate secondary antibody (rabbit, Proteintech; goat, Thermo Scientific; mouse, Proteintech; goat, Piece) at a 1:100000 dilution in a chemiluminescent blocking buffer ...
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bioRxiv - Cell Biology 2020Quote: ... followed by secondary antibodies (Alexa Fluor Goat anti-Rat 488 Invitrogen #A-11006, 1:1000 and Alexa Fluor Goat anti-Rabbit 594 Invitrogen #A-11012, 1:1000). Coverslips were mounted on slides with Fluoromount-G mounting media containing DAPI (Southern Biotech ...
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bioRxiv - Microbiology 2020Quote: ... Ultrathin sections of 50 nm were blocked with 5% fetal bovine serum/5% normal goat serum for 30 min and subsequently incubated with rabbit anti-GFP antibody (Life Technologies Corp., Eugene, OR) for 60 min at room temperature ...
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bioRxiv - Molecular Biology 2021Quote: ... Membrane was washed twice with 0.1% PBST and incubated in anti-rabbit or anti-mouse secondary HRP-labeled secondary antibody (ThermoFisher Scientific 31460, 31430 1:100000) for 1 hour at room temperature ...
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bioRxiv - Developmental Biology 2021Quote: ... Secondary antibodies and stains: Goat anti-Rabbit IgG (H+L) Alexa 488 and 594 (1:1000 cells, 1:500 tissue Invitrogen; A-11008, A-11012), Goat anti-Mouse IgG1 Alexa 488 (1:500 Invitrogen ...
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bioRxiv - Neuroscience 2022Quote: ... Secondary antibodies (anti-rabbit Alexa Fluor 555 Plus, 1:200, Thermo Fisher A-32732, anti-chicken Alexa Fluor 488, 1:200, Thermo Fisher A-11039) and nuclear dye (TO-PRO-3 Iodide ...
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bioRxiv - Neuroscience 2021Quote: ... cells were washed with PBS and further incubated in the dark for 2 h with Alexa Fluor® 488-conjugated donkey anti-rabbit IgG secondary antibody (Thermo Scientific; 1:500). Then ...
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bioRxiv - Developmental Biology 2020Quote: ... and incubated for 30 minutes at room temperature with a 1:1000 dilution of donkey anti-rabbit IgG (H+L) secondary antibody conjugated to Alexa Fluor® 647 (ThermoFisher-Invitrogen, catalog A-31573) in blocking solution ...
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bioRxiv - Plant Biology 2019Quote: ... Aliquots were examined on a 10% SDS-PAGE followed by Coomassie Blue staining or Western Blot analysis with rabbit α-GST-HRP antibody (Life Technologies, Carlsbad, CA) at a 1:50,000 dilution in Tris-buffered saline (20 mM Tris ...
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bioRxiv - Developmental Biology 2019Quote: ... we washed the cryosections and incubated them in secondary antibody (goat anti-rabbit conjugated to Alexa Fluor 568, 1:200, Molecular Probes, no. A-11011) at 4 °C overnight ...
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bioRxiv - Developmental Biology 2019Quote: ... The secondary antibodies used were donkey anti-rabbit IgG Alexa Fluor 488 and goat anti-mouse IgG Alexa Fluor 555 (Molecular Probes A21206 and A21424).
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bioRxiv - Neuroscience 2019Quote: ... The secondary antibody Alexa Fluor®568 anti-rabbit IgG and Alexa Fluor®633 anti-mouse IgG (1:1000; Invitrogen, Eugene, OR, USA) were applied ...
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bioRxiv - Neuroscience 2019Quote: ... The following secondary antibodies were applied: Alexa Fluor® 488 and 568 anti-mouse or anti-rabbit IgG (1:1000; Invitrogen, Eugene, OR, USA).
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bioRxiv - Cell Biology 2021Quote: ... Cells were then incubated with horseradish peroxidase (HRP)-conjugated secondary antibodies (1:1000, chicken-anti mouse IgG Invitrogen Cat# A15975 or chicken-anti rabbit IgG Invitrogen Cat# A15987, Table 4) in cell culture medium containing 10% FBS for 1 h at RT ...
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bioRxiv - Neuroscience 2020Quote: ... Secondary antibodies used were α-mouse or -rabbit Alexa Fluor 488-conjugate raised in goat (1:300, Invitrogen A-11001 or A-11008, respectively), and α-mouse or rabbit Alexa Fluor 594-conjugate raised in goat (1:300 ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... Slides were thoroughly washed the next day then incubated in goat anti-rabbit IgG Alexa Fluor 647 secondary antibody for 1 hour at room temperature (ThermoFisher A-21245, 1:1000). Slides were thoroughly washed then mounted with ProLong Gold Antifade with DAPI (Cell Signaling Technology 8961) ...
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bioRxiv - Neuroscience 2020Quote: ... samples were incubated overnight at +4C with goat anti-rabbit Lyve-1 and Prox-1 antibody (1:500; Invitrogen, Molecular Probes, Eugene, Oregon, USA). After several rinses in PBS ...
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bioRxiv - Neuroscience 2020Quote: ... samples were incubated overnight at +4C with goat anti-rabbit Lyve-1 and Prox-1 antibody (1:500; Invitrogen, Molecular Probes, Eugene, Oregon, USA). After several rinses in PBS ...
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bioRxiv - Neuroscience 2020Quote: ... Cells were then incubated overnight at 4°C in blocking solution (PBS, 2% normal goat serum, 3% BSA) and primary antibodies (mouse anti-HA, Invitrogen; rabbit anti-GFP, Invitrogen). Cells were then immunolabeled with Alexa-conjugated secondary antibodies (Thermo Fisher Scientific ...
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bioRxiv - Molecular Biology 2021Quote: ... The bound antibodies were detected by incubation with Alexa Fluor 488-conjugated donkey anti-rabbit IgG (Thermo Fisher Scientific, Rockford, IL, USA; 1:300) or Alexa Fluor 546-conjugated donkey anti-goat IgG (Thermo Fisher Scientific ...
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bioRxiv - Cell Biology 2021Quote: ... Cells were then washed with PBS thrice and incubated with diluted anti-rabbit secondary antibody conjugated with Alexa Fluor 594 (Thermo Fisher Scientific, A-11072) at 1:1,000 for 1 h at room temperature ...
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bioRxiv - Genomics 2021Quote: ... followed by washes with PBS before the addition of the secondary antibody (goat anti-rabbit or mouse IgG conjugated to Alexa Fluor 594, (Life Technologies, A11012 and A11005), diluted 1:500 in 0.5% BSA ...
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bioRxiv - Biochemistry 2021Quote: ... the membrane was incubated with the HRP-conjugated anti-rabbit secondary antibodies at room temperature for 1 h and developed using Pierce™ ECL Western Blotting Substrate (Thermo Fisher Scientific, #32106). The images were captured on ChemiDoc™ Touch Imaging System (Bio-Rad) ...
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bioRxiv - Cell Biology 2021Quote: ... slides were washed and incubated with Alexa Fluor 546 goat anti-rabbit or Alexa Fluor 488 goat anti-mouse secondary antibodies (Life Technologies, A11001 and A11010) at room temperature for 1 h ...
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bioRxiv - Neuroscience 2020Quote: ... washed in the permeabilizing solution for 1 day, and incubated in the secondary antibody (goat anti-rabbit Alexa Fluor 488, 1:200) and Streptavidin-Alexa 594 (ThermoFisher Scientific S11227, 1:100) for 6 days at 4°C.
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bioRxiv - Neuroscience 2020Quote: ... Samples were washed three times in PBS-T and were incubated for 2 hr with the secondary antibodies Alexa Fluor® 488 or 546 Goat Anti-Mouse/Rat/Rabbit IgG (Life Technologies; 1:200) and then with DAPI (Life Technologies ...
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bioRxiv - Neuroscience 2020Quote: ... 0.05% Tween-20 in Tris-buffered saline followed by overnight incubation with the following antibodies: rabbit-anti-HDAC1 (Thermo Fisher; PA1-860; 1:2000), HDAC1 phosphorylation on serine residue 421 (rabbit-anti-pHDAC1(S421) ...
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bioRxiv - Physiology 2021Quote: ... sections were washed three times with phosphate-buffered saline containing 0.1% Tween-20 for 5 min and then incubated in secondary antibody (goat anti-rabbit AlexaFluor® 647, Thermo Fisher Scientific (Waltham, Massachusetts), Lot 1910774 for laminin ...
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bioRxiv - Neuroscience 2021Quote: ... Slices were washed with PBS and then secondary antibodies applied (Goat anti-rabbit AlexaFluor 488 and Goat anti-mouse AlexaFluor 633, Invitrogen, UK, both 1:500) in PBS with 3% normal goat serum ...
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bioRxiv - Cancer Biology 2021Quote: ... the sections were incubated with secondary antibody 1:1000 (Alexa Flour 594 goat anti-rabbit IgG and Alexa Flour 488 goat anti-mouse IgG (Invitrogen #A11037 and #A10680 respectively)) for 1 h at room temperature followed by washing thrice ...
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bioRxiv - Cancer Biology 2022Quote: ... the slices were incubated with a secondary goat-anti-mouse or rabbit Alexa-488 or Alexa 563 antibodies or goat-anti-mouse-HRP (Invitrogen, Life Technologies, Carlsbad, CA) for 2 h followed by DAPI cell nuclei staining or Hematoxylin staining ...
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bioRxiv - Synthetic Biology 2022Quote: ... Bound Q-GRFT was detected using an HRP-conjugated goat anti-rabbit polyclonal antibody at 1:5000 dilution and TMB (Cat. #65-6120 and #N301, respectively, Thermo Fisher Scientific, Waltham, MA).
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Targeting Noradrenergic Neurons of the Locus Coeruleus: A Comparison of Model Systems and StrategiesbioRxiv - Neuroscience 2022Quote: ... slices were incubated with secondary antibodies (Alexa Fluor 546 goat anti-rabbit, A11035, Invitrogen, Thermo Fisher Scientific; and Alexa Fluor 488 goat anti-chicken, A11039, Invitrogen, Thermo Fisher Scientific) diluted 1:1000 in the above-mentioned carrier solution over night at 4°C ...