Labshake search
Citations for Thermo Fisher :
7501 - 7550 of 10000+ citations for Aldosterone Chemiluminescent ELISA Kit 5 Plate since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Biochemistry 2021Quote: OD600 and GFP measurements were made using a Tecan Infinite 200 plate reader and 200 ul black-walled plates purchased from Fisher Scientific (Hampton, NH; Cat#07-000-136) at a 40-fold dilution ...
-
bioRxiv - Plant Biology 2020Quote: ... the sample was loaded on a trap column (C18 PepMap 100, 300 µm x 5 mm, 5 mm particle size, 100 Å pore size; Thermo Scientific) and desalted for 5 min using 0.05 % (v/v ...
-
bioRxiv - Cancer Biology 2021Quote: ... clone IMAGE ID 4977050 was obtained from Source Bioscience and PCR cloned using oligos (Tet2fwd: 5’-GGGGACAAGTTTGTACAAAAAAGCAGGCTTAatgccaaatggcagtacagt-3’ and Tet2rev: 5’-GGGGACCACTTTGTACAAGAAAGCTGGGTTtcatacaaatgtgttgtaag-3’) into pDonor221 (Invitrogen Gateway, ThermoFisher) and sequenced ...
-
bioRxiv - Cell Biology 2020Quote: ... or a 1:1 mixture of two siRNA to CHMP2A (5’-CAGGCCGAGAUCAUGGACAUG-3’ and 5’-GAAGAUGAAGAGGAGAGUGAC-3’) using Lipofectamine 2000 (Thermo Fisher Scientific) according to the manufacturer’s recommendations ...
-
bioRxiv - Cell Biology 2020Quote: ... 5 µg DNA was used per flask (in optiMEM) and 5 hours after transfection replaced by F10-DMEM media (Thermo Fisher Scientific). 48 hours post-transfection cells were washed with PBS and scraped off ...
-
bioRxiv - Developmental Biology 2021Quote: ... and the reverse primer containing the Sp6 promoter (5’ ATTTAGGTGACACTATAGCCTCTTCAGTTCCTTCTTCCATC-3’).The aqp1a.1 probe was generated from whole extracted cDNA at 30hpf and amplified with the primers (5’-GTCATGAACGAGCTGAAGAGC-3’) and (5’-GGGTCACTTTGAGGACATCTC-3’),incorporated into the pCR-BluntII-TOPO vector (Invitrogen, 45-0245) (containing both the SP6 and T7 promoters) ...
-
bioRxiv - Developmental Biology 2020Quote: ... A volume of 5 µL was loaded onto a 5 mm C18 trap column (Acclaim PepMap 100, 5 µm particles, 300 µm inside diameter, Thermo Fisher Scientific) at 20 µL/ min for 2.5 min in 99% phase A (0.1% formic acid in water ...
-
bioRxiv - Molecular Biology 2020Quote: ... and shRNA plasmids or pCDH plasmids by PEI-40000 with the ratio of 5: 1: 5 in opti-MEM (Gibco, Thermo Fisher Scientific). Virions were collected after 48 h after transfection ...
-
bioRxiv - Molecular Biology 2021Quote: ... Cas9 as well as the flanking 5’ and 3’ nuclear localizing sequences and 5’-V5 tag from the GeneArt CRISPR Nuclease Vector (Thermo Fisher Scientific). HEK293-Cas9 cells were maintained in DMEM supplemented with 10% FBS ...
-
bioRxiv - Neuroscience 2019Quote: ... were loaded at 5 μL/min onto a trap column (100 μm × 2 cm, PepMap nanoViper C18 column, 5 μm, 100 Å, Thermo Scientific) equilibrated in 98% buffer A ...
-
bioRxiv - Neuroscience 2019Quote: ... mice were euthanized and both striatum were dissected immediately and homogenized using a glass dounce homogenizer (5 loose and 5 tight strokes) in buffer A of mitochondria isolation buffer (Thermo Scientific 89874). and kept on ice for 2 minutes ...
-
bioRxiv - Genomics 2020Quote: ... The 16S rRNA gene was amplified from the extracted genomic DNA using 16S universal primers 27F (5’-AGA GTTTGATCMTGGCTCAG-3’) and 1492R (5’-GGTTACCTTGTTACGACTTC-3’) and sequenced using an automated ABI3730XL capillary DNA sequencer (Applied Biosystems, USA) for taxonomic identification at Cosmo Genetech (Seoul ...
-
bioRxiv - Neuroscience 2021Quote: ... were loaded into a µ-Precolumn (300µm i.d x 5 mm, C18 PepMap100, 5 µm, 100 Å, C18 Trap column; Thermo Fisher Scientific) at a flow rate of 15 µL/min and separated using a C18 analytical column (Acclaim PepMap TM RSLC ...
-
bioRxiv - Biochemistry 2020Quote: ... Peptides were loaded into a PepMap C18 (5 mm × 300 μm ID, 5 μm particles, 100 Å pore size; Thermo Fisher Scientific) trap column on an UltiMate 3000 RSLC nano HPLC system (Thermo Fisher Scientific ...
-
bioRxiv - Biochemistry 2020Quote: ... The samples were loaded onto a trapping column (Acclaim PepMap C18, 300 μm x 5 mm, 5 μm, 100Å, Thermo Fisher Scientific) and washed for 15 min with 0.1 % (v/v ...
-
bioRxiv - Cell Biology 2020Quote: ... Samples were pre-concentrated on a cartridge trap column (300 μm i.d. × 5 mm) packed with C18 PepMap100 sorbent with 5 μm particle size (Thermo Scientific, MA, USA) using a mobile phase composed from 0.05% trifluoroacetic acid (TFA ...
-
bioRxiv - Biochemistry 2021Quote: ... followed by 10 cycles of 5’ 60 °C -> 5’ 24 °C (HDV cleavage of construct to release pure R3C) and DNAse I treatment (Thermo Scientific, USA). 75-85 nt randomised oligomer was expressed using artificial DNA template (Eurofins Genomics ...
-
bioRxiv - Microbiology 2020Quote: ... Peptides were loaded onto an Acclaim PepMap100 C18 trap column (300 µm ID × 5 mm, 5 µm, Thermo Fisher Scientific; Cat. # 160454) and then separated on a PepMap RSLC C18 analytical column (75 µm ID×15 cm ...
-
bioRxiv - Microbiology 2020Quote: ... 229E-RP reverse primer (5’-CCAACACGGTTGTGACAGTGA-3’) and the TaqMan minor groove binder (MGB) probe 229E-TP (FAM-5’-TCCTGAGGTCAATGCA-3’-NFQ-MGB; Thermo Fisher Scientific), derived from the HCoV 229E membrane protein gene sequence as described previously (Vijgen et al. ...
-
bioRxiv - Evolutionary Biology 2020Quote: ... with two repetitive DNA sequences Oligo-pSc119.2 labeled with Alexa Fluor 488-5-dUTP (green coloration) and Oligo-pTa535 with Texas Red-5-dCTP (red coloration) (synthesized by Invitrogen, Shanghai, China), which have successfully identified all 21 homologous chromosomes pairs of Chinese Spring (CS ...
-
bioRxiv - Microbiology 2021Quote: ... Samples were concentrated onto the trap column at 5 μl/minute with Buffer A for 5 minutes and infused into an Orbitrap Elite™ Mass Spectrometer (Thermo Scientific) at 300 nl/minute via the analytical column using an Dionex Ultimate 3000 UPLC (Thermo Scientific) ...
-
bioRxiv - Microbiology 2021Quote: The mcr-1 gene was amplified from the clinical isolate ESBL20150072 (European Nucleotide Archive, accession number; SAMEA104060671) using primers: 5’-TTTTGGATCCCGGTTTTCGGGCTTTTTA-3’ and 5’-ATATGTCGACTCAGCGGATGAATGCGGT-3’ and Phusion polymerase (Thermo Fisher Scientific). PCR product was digested with BamHI and SalI and ligated into pACYC184 digested with the same enzymes (Thermo Fisher Scientific).
-
bioRxiv - Microbiology 2021Quote: ... cells were incubated upon shaking at the growth temperature for 5 min with following concentrations: FM 5-95 (2 µg/ml; Thermo Fisher Scientific), DiSC3(5 ...
-
bioRxiv - Microbiology 2020Quote: ... consisting of a reversed-phase trap column (Acclaim PepMap100 C18, 5 μm, 100 Å, 300 μm i.d. × 5 mm, Thermo Fisher Scientific) and a reversed-phase analytical column (30 cm ...
-
bioRxiv - Microbiology 2020Quote: ... COL1A1 (5’-CGAAGACATCCCACCAATC-3’ and 5’-ATCACGTCATCGCACAACA-3’), ALP (5’-TCACTCTCCGAGATGGTGGT-3’ and 5’-GTGCCCGTGGTCAATTCT-3’) (IDT, Coralville, USA) and viral non-structural (nsP1) gene (Applied Biosystems, USA) (5’-GGGCTATTCTCTAAACCGTTGGT-3’ and 5’-CTCCCGGCCTATTATCCCAAT-3’ ...
-
bioRxiv - Cell Biology 2021Quote: ... 100 million HEK293T cells were seeded into a 15 cm2 plate with complete DMEM and transfected with 5 μg of lentiviral plasmid packaging mix and 5 μg of pooled sgRNA library plasmid using lipofectamine 2000 (ThermoFisher Cat# 11668019) and incubated for 2 days.
-
bioRxiv - Cell Biology 2021Quote: ... PCSK9 mRNA expression was assessed by real-time PCR using PCSK9 primer sets 5’-TGTCTTTGCCCAGAGCATC-3’ and 5’-GTCACTCTGTATGCTGGTGTC-3 (Integrated DNA Technologies) and SYBR Select Master Mix (ThermoFisher, ABI 4472908).
-
bioRxiv - Cell Biology 2020Quote: ... Peptides were loaded onto a micro precolumn (300 μm i.d. × 5 mm, particles were C18 PepMap 100, 5 μm particle size, 100 Å pore size, Thermo Fisher Scientific) using the loading pump for 3 min ...
-
bioRxiv - Biophysics 2021Quote: ... Thermo Scientific Tomography 5 (Tomo 5) is the official solution provided with Thermo Scientific microscopes for electron tomography experiments (ThermoFisher Scientific, 2020). We provide a small command line tool mdocspoofer for the generation of these metadata files for a directory containing multi-frame micrograph files of the form
_ [ ]_fractions.mrc ... -
bioRxiv - Microbiology 2022Quote: ... 2.5 x 107, or 5 x 107 PFU/mL (MOI of 5, 50, or 100 respectively) in CO2-independent medium (Gibco Life Technologies) supplemented with 0.1% (w/v ...
-
bioRxiv - Genetics 2022Quote: ... 5’-GGCAAGCUGACCCUGAAGUdTdT-3’ / 5’-ACUUCAGGGUCAGCUUGCCdTdT-3’ or with a hsa-miR-34a mimic duplex: 5’-P-UGGCAGUGUCUUAGCUGGUUGUU-3’ / 5’-P-CAAUCAGCAAGUAUACUGCCCUA-3’ according to the protocol for Lipofectamine 2000 Transfection Reagent (Thermo Fisher Scientific).
-
bioRxiv - Cell Biology 2022Quote: ... an equivalent of 1 μg total protein starting material was injected using the μL-pickup method with 0.1% v/v formic acid as a transport liquid from a cooled autosampler (5 °C) and loaded onto a trap column (μPrecolumn cartridge, Acclaim PepMap100 C18, 5 μm, 100 Å, 300 μmx5 mm, Thermo Scientific). Peptides were separated on the nano-LC column using a linear gradient from 2-45% v/v acetonitrile plus 0.1% v/v formic acid in 85 min at a flowrate of 300 nL/min ...
-
bioRxiv - Cell Biology 2022Quote: ... and loaded onto a trap column (μPrecolumn cartridge, Acclaim PepMap100 C18, 5 μm, 100 Å, 300 μm id, 5 mm Thermo Scientific). Peptides were separated on the nano-LC column using a linear gradient from 3-60 % v/v acetonitrile plus 0.1% v/v formic acid in 100 minutes at a flowrate of 300 nL/min ...
-
bioRxiv - Cell Biology 2021Quote: ... The sgRNA sequences targeting CD33 exon 2 were 5’-TCCATAGCCAGGGCCCCTGT and 5’-GCATGTGACAGGTGAGGCAC.25 U937 cells were washed three times in PBS (Gibco 10010-023) and resuspended in complete Nucleofector Kit C (Lonza Biosciences VCA-1004 ...
-
bioRxiv - Cell Biology 2022Quote: ... injected with a full sample loop injection of 5 µL into a C18 Pepmap trap column (20 × 100 μm i.d., 5 μm, 100 Å; Thermo Fisher Scientific), and separated in EASY-Spray column (500 × 75 μm i.d. ...
-
bioRxiv - Plant Biology 2019Quote: ... The sample was loaded on a trap column (C18 PepMap 100, 300 μm x 5 mm, 5 mm particle size, 100 Å pore size; Thermo Scientific) and desalted for 5 min using 0.05 % (v/v ...
-
bioRxiv - Pathology 2020Quote: ... Aliquots of 15 μL per sample were loaded at a rate of 5 μL/min onto a trap column (100 μm × 2 cm, PepMap nanoViper C18 column, 5 μm, 100 Å, Thermo Scientific) which was equilibrated with 98% Buffer A ...
-
bioRxiv - Cell Biology 2019Quote: Cultured mCSCs and mBMSCs were suspended at 5 × 10^5 cells/mL in PBS and 200uL was loaded into EZ Cytofunnels (ThermoFisher, Carlsbad, CA) within a Shandon Cytospin 4 (ThermoFisher ...
-
bioRxiv - Genomics 2019Quote: We cotransfected 10-12 replicates of HCT-116 cells with 5 µg of PB-SRT-Puro plasmid and 5 µg PBase plasmid via Neon electroporation (Thermo Fisher #MPK10025). Each replicate contained 2×106 cells ...
-
bioRxiv - Cell Biology 2020Quote: ... the gel bands were incubated in 150 μL of 100% ACN for 5 minutes at RT and dried for 5 minutes in a SpeedVac Vacuum Concentrator (Thermo Fisher Scientific). Ingel digestion was performed overnight in 50 μL reactions containing 12.5 ng/μL trypsin in 50 mM TEAB at 30°C ...
-
bioRxiv - Molecular Biology 2021Quote: ... After 5 h incubation at 37°C with 5% CO2 cell were washed twice with PBS containing proteases inhibitors (Thermo Fisher Scientific), spun down at 1000 g for 5 min at 4°C and snap frozen in liquid nitrogen ...
-
bioRxiv - Microbiology 2020Quote: ... The negative controls of our RT-PCR reactions were formed from the same reaction mix as our samples switching only the 5 uL of DNA by 5 uL of ultrapure™ DNase/RNase-Free Distilled Water (Invitrogen France), thus one negative control is placed after every 5 samples on a Light cycler 480 multiwell plates 96-well plate (Roche).
-
bioRxiv - Microbiology 2020Quote: ... and hyphae were washed three times with PBS (pH 7.4) and then incubated in 5 mL of PBS containing 5 mg EZ-Link Sulfo-NHS-LC-Biotin (Thermo Fisher Scientific, 21335) for 1 h at 4°C ...
-
bioRxiv - Developmental Biology 2020Quote: ... Peptides were loaded onto a trap column (PepMap C18, 5 mm × 300 μm ID, 5 μm particles, 100 Å, Thermo Fisher Scientific) at a flow rate of 25 μL min−1 using 0.1% TFA as mobile phase ...
-
bioRxiv - Cancer Biology 2019Quote: ... (5’-CUAUGAACAUAAAGUCUGCTT-3’) or its non-specific control (5’-UUCUCCGAACGUGUCACGUTT-3’) were transfected into cells using Lipofectamine 3000 reagent (Invitrogen, Carlsbad, CA).
-
bioRxiv - Molecular Biology 2019Quote: ... supplemented with protease inhibitors (1 mM PMSF, 5 μg/ml aprotinin and 5 μg/ml leupeptin, #78443, Thermo Fisher Scientific, Waltham, MA). Fifty ul of Dynabeads Protein A/Protein G (#10015D ...
-
bioRxiv - Microbiology 2019Quote: ... free bacteria were washed off and the infected cells were resuspended in Hl5c containing 5 U/mL of penicillin and 5 μg/ml streptomycin (Thermo Fisher Scientific). The time of addition of bacteria to the adherent cells is referred to as 0 hours post infection (hpi) ...
-
bioRxiv - Neuroscience 2021Quote: ... Drosophila dSF2 sequence was PCR amplified (dSF2 F 5’-CACCATGGGATCACGCAACGAGTGCCG-3’ and dSF2 R 5’- ATAGTTAGAACGTGAGCGAGACCTGG-3’) was cloned into pEntry-TOPO vector (Thermo Fisher Scientific). The pENTR-dSF2 vector was recombined with Gateway plasmid pTWH (Drosophila Genomics Resource Center ...
-
bioRxiv - Physiology 2021Quote: ... To load 5 µl of sample peptide was aspirated into a 50 µl loop and then loaded onto the trap column (Acclaim PepMap100 C18, 5 μm, 100 Å, 300 μm i.d. × 5 mm Cat #160454, Thermo Fisher Scientific). Separation on an analytical column (Acclaim PepMap RSLC 75 μm ...
-
bioRxiv - Cell Biology 2020Quote: ... organoids (after 1 week of culture) were incubated (37°C, 5% CO2, 1 h) with 10 µM EdU (5-ethynyl-2’-deoxyuridine, Thermo Fisher Scientific), a nucleoside analog of thymidine incorporated into DNA during active DNA synthesis ...