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Citations for Thermo Fisher :
7701 - 7750 of 10000+ citations for 2 3 5 6 Tetrahydroxy 4 phosphonooxycyclohexyl dihydrogen phosphate since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... (QuantStudio 3, Applied Biosystems, Thermo Fisher) in a 96-well plate (USA Scientific ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... (QuantStudio 3, Applied Biosystems, Thermo Fisher) in a 96-well plate (USA Scientific ...
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bioRxiv - Molecular Biology 2024Quote: ... and laminin (3 μg/mL; ThermoFisher), and placed in a humidified CO2 incubator for 15 min ...
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bioRxiv - Microbiology 2024Quote: ... Glass beads (3 mm; Fisher Scientific) and 1 ml fecal lysis buffer (50 mM Tris pH 7.6 ...
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bioRxiv - Genetics 2024Quote: ... and QuantStudio 3 (Thermo Fisher Scientific) with 10 nM of each primer at a final volume of 10 µL ...
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bioRxiv - Bioengineering 2024Quote: ... 3 μl of Lipofectamine MessengerMAX (Invitrogen) was diluted in 100 μl of Opti-MEM I ...
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bioRxiv - Cancer Biology 2024Quote: ... and Quant Studio 3 (Applied Biosystems). The following primers were used for qPCR ...
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bioRxiv - Cell Biology 2024Quote: ... 3) RNAlater (ThermoFisher Scientific; Cat#AM7021), 4 ...
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bioRxiv - Cell Biology 2024Quote: ... supplemented with 3% FBS (Gibco BRL) and penicillin/streptomycin (Gibco BRL ...
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bioRxiv - Developmental Biology 2024Quote: ... and 3 mM KCN (Thermo Scientific) for 60 min each.
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bioRxiv - Biophysics 2022Quote: Isolated islets were stained with 4 mM Fluo-4 AM (Invitrogen, Carlsbad, CA) in imaging medium (125 mM NaCl ...
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bioRxiv - Bioengineering 2024Quote: ... and Octamer-binding transcription factor 4 (OCT-4; 1:40, Invitrogen, MA5-14845), were incubated for 24 hours ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... 100 µl of assay buffer containing 4 µM Fluo-4-AM (Life Technologies), 0.02 % Pluronic F127 and 2.5 mM probenecid in HBSS-Ca2+ was added to each well and the plate was incubated at room temperature (RT ...
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bioRxiv - Cell Biology 2024Quote: ... were loaded with cell-permeant 4 µM Fluo-4 AM (Invitrogen, F-14201) for 35 minutes ...
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bioRxiv - Molecular Biology 2020Quote: ... RNA was extracted from the amygdala of female offspring at P7 and P90 (n=6/mCHD, n=6/mHFD) using TRIzol Reagent (15596018; ThermoFisher Scientific, Ottawa, ON, CAD) according to manufacturer’s instructions ...
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bioRxiv - Cell Biology 2020Quote: ... or 52- or 78-week-old C57BL/6 mice or C57BL/6-TgTM4SF5 mice by perfusion of the liver using collagenase type II (Life Technologies, Carlsbad, CA, USA) (Ateş et al ...
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bioRxiv - Immunology 2023Quote: ... Monocytes were harvested from 6-week-old C57BL/6 mice and were differentiated into dendritic cells using supplemented culture medium: RPMI 1640 (Life Technologies, Waltham, Massachusetts, USA), 10% HIFBS (Sigma-Aldrich ...
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bioRxiv - Bioengineering 2022Quote: ... pulsing 2 s on and 4 s off for a total of 10 min of “on” time (Fisher Scientific, catalog no. FB120A220, probe CL-18). Note that from harvesting onward the libraries were kept at 4 °C ...
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bioRxiv - Molecular Biology 2020Quote: ... 2 (Thermofisher). A reverse transfection approach was used to deplete AurkA and CEP135 ...
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bioRxiv - Systems Biology 2023Quote: ... 2% (ThermoFisher). Purified amplicons were quantified by Qubit™ dsDNA Quantification Assay Kits (ThermoFisher ...
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bioRxiv - Neuroscience 2023Quote: ... for 2 minutes followed by ice cold 4% PFA for 4 min with a flow rate of 6ml/min and post-fixed (overnight at 4°C) in 4% PFA (Thermo Fisher Scientific) in 1x PBS (pH 7.4) ...
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bioRxiv - Genomics 2023Quote: ... cells were washed 3 times with PBS for 5 min and stained with donkey anti-goat Alexa Fluor® conjugated secondary antibody (Thermo Fisher Scientific Inc., Cat. No. A110-55) at 1:500 for 1 hour at room temperature in the dark ...
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bioRxiv - Immunology 2021Quote: ... were co-transfected with the EGFP-Caspase 2 or EGFP-Δ Caspase 2 plasmid and the synthesized miR-2 (miR-2-mimic) using Cellfectin II transfection reagent (Invitrogen) according to the manufacturer’s protocol ...
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bioRxiv - Biochemistry 2022Quote: ... and 5 µg/mL and 5 units/mL penicillin-streptomycin (Gibco). One T175 flask of HEK293 cells of 70% confluency per condition was transiently transfected using 90 µg of EGFP-PABPi or EGFP-PABPi mut ...
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bioRxiv - Neuroscience 2021Quote: ... 5 U/ml penicillin and 5 U/ml streptomycin (Invitrogen, USA). Cells were plated on poly-D-lysine (Sigma-Aldrich ...
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bioRxiv - Cell Biology 2021Quote: ... using forward primer 5’-CACAGGAAGCCCTGGAAGCT and reverse primer 5’-GAGCAGGTCAGGTTTTTGGA (Invitrogen). SIGLEC22P was amplified similarly with forward primer 5’-GCACCTCAGAGTGGAAGGAC and reverse primer 5’-GAAGGGGTGACTGAGGTACA ...
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bioRxiv - Cell Biology 2021Quote: ... with forward primer 5’-GCACTTGCAGCCGGATTTTTGGATCCATAGCCAGGGCC and reverse primer 5’-GGCCCTGGCTATGGATCCAAAAATCCGGCTGCAAGTGC (Invitrogen) to generate the pcDNA3.1-KIR-CD33-V5/HIS vector ...
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bioRxiv - Biochemistry 2024Quote: hSSB2 was labeled with 5-IAF (5-iodoacetamido-fluorescein, Thermo Fisher) on the intrinsic cysteines ...
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bioRxiv - Cell Biology 2023Quote: ... and 5 µL of 5% ammonium persulfate (APS) (Thermo Scientific, 17874). 50 µl was added to a strip of parafilm in a humidity chamber on ice ...
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bioRxiv - Biophysics 2023Quote: ... samples were incubated with a 5 μM DRAQ-5 (Thermo Scientific) DNA staining buffer at 37° C for 30 minutes with agitation ...
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bioRxiv - Biochemistry 2024Quote: ... 0.3 × 5 mm trapping column (5 µm, 100 Å, Thermo Scientific) and analyzed by nanoLC-ESI-MS/MS analysis using a Thermo Ultimate 3000 RSLC nanoUPLC coupled online to an Orbitrap Exploris™ 240 mass spectrometer (Thermo Fisher Scientific) ...
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bioRxiv - Microbiology 2024Quote: ... containing 5 unit/mL penicillin and 5 ug/mL streptomycin (Gibco).
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bioRxiv - Genetics 2021Quote: ... The expression levels of candidate genes were then quantified through qPCR using the 2 × T5 Fast qPCR Mix (TsingKe, Beijing, China) on an ABI QuantStudio 5 system (Thermo Fisher, Waltham, MA, USA). The GAPDH gene was used as the internal reference and the final volume of the qPCR reaction was 20 μl ...
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bioRxiv - Cancer Biology 2021Quote: Cell proliferation was examined by 5-bromo-2-deoxyuridine (BrdU) cell proliferation enzyme-linked immunosorbent assay (ELISA) according to the kit manufacturer’s protocol (Life Technologies, Grand Island, NY, USA). The cells were incubated in presence of various concentrations of CLEFMA (0 – 10 µM) ...
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bioRxiv - Neuroscience 2020Quote: ... HEPES media for 30 minutes at 37°C with 5% CO2 and 2 drops/mL of NucBlue™ Live ReadyProbes™ Reagent (Invitrogen™, R37605) for 20 minutes ...
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bioRxiv - Biochemistry 2020Quote: The MuSIC-probe labeled antibodies from above are suspended in 200 ml of 0.02% (2 ml/10ml) Tween 20 (Fisher Scientific Cat: 9005-64-5) in PBS and added to 50 ml of protein A dynabeads (Fisher Scientific Cat ...
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bioRxiv - Pathology 2020Quote: ... Acclaim PepMap, 100 μm × 20 mm, 5 μm, 100 Å, separation column: Acclaim PepMap, 75 μm × 500 mm, 2 μm, 100 Å, Thermo Fisher Scientific) equipped with the Dionex UltiMate 3000 RSLCnano System (Thermo Fisher Scientific ...
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bioRxiv - Microbiology 2020Quote: ... and SARS-CoV-2 RdRp IP2-targeted RdRp Institute Pasteur RT-PCR was performed on a QuantStudio™ 5 System (Applied Biosystems, Thermo Fisher Scientific). Log10 reduction values (LRV ...
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bioRxiv - Biochemistry 2020Quote: ... The nanoLC system was equipped with an Acclaim™ Pepmap™ 100 C18 nano-trap column (1×2 cm, 5 μm particle size, Thermo Scientific, USA) and an Acclaim™ Pepmap™ 100 C18 analytical column (length 15 cm ...
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bioRxiv - Neuroscience 2022Quote: ... slides were rinsed with 0.025% Triton X-100/1xTBS 2 × 5 min and incubated in goat anti-rabbit AlexaFlour488 secondary antibody (1:1000, Life Technologies, #A27034, Carlsbad, CA) at RT for at least 2 h followed by 4’,6-diamidino-2-phenylindol (DAPI ...
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bioRxiv - Immunology 2022Quote: We estimated hemocyte differentiation by visualizing and quantifying the synthesis of new DNA in vivo based on 5-ethynyl-2′-deoxyuridine (EdU) incorporation and subsequent detection using the Click-iT EdU Alexa Fluor 647 kit (Invitrogen, Grand Island, NY, USA) as previously described (36,37) ...
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bioRxiv - Plant Biology 2022Quote: ... The seedlings were screened by microscopy for YFP and then fixed and processed for immunofluorescence microscopy using a 1:2000 dilution of monoclonal anti-α-tubulin B-5-1-2 antibody (Life Technologies; 32-2500) followed by 1:2000 dilution of Alexa-568 goat anti-mouse antibody (Thermo Fisher ...
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bioRxiv - Plant Biology 2023Quote: ... Peptides were reconstituted in 0.1% trifluoroacetic acid (TFA) and 2% acetonitrile (ACN) and loaded onto a PepMap C18 5 µm 1 cm trapping cartridge (Thermo-Fisher Scientific, Waltham, MA, USA) at 12 µL/min for 6 min before switching the pre-column in line with the analytical column (nanoEase M/Z Peptide BEH C18 Column ...
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bioRxiv - Molecular Biology 2022Quote: ... Cell proliferation was also assessed by 5-ethynyl-2’-deoxyuridine (EdU) incorporation assay using Click-iT™ EdU Alexa Fluor™ 647 Flow Cytometry Assay Kit (Thermo Fisher Scientific) according to the manufacturer’s instructions.
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bioRxiv - Molecular Biology 2024Quote: ... The gel was prepared by adding 5 µL of the PCR probes and 2 µL of the ladder (Thermo Fisher Scientific, Waltham, MA, USA) and running the reaction at 90 V for 2 hours and 45 minutes ...
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bioRxiv - Neuroscience 2024Quote: Cultured sensory neurons from wild-type and TRESK-/- mice were loaded with 5 μM fura-2/AM (F1221, Invitrogen, ThermoFisher Scientific, Waltham, MA, USA) for 45-60 min at 37°C in culture medium ...
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bioRxiv - Microbiology 2023Quote: ... Bodies and legs/wings were placed into 2-mL tubes containing 500 µL PBS and a 5-mm glass bead (Thermo Fisher Scientific, Emeryville, CA), then homogenized at 30 hertz for 5 minutes in a TissueLyser (Retsch ...
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bioRxiv - Molecular Biology 2022Quote: ... and U-2 OS cells were cultured at 37°C in 5% CO2 and maintained in DMEM supplemented with 10% FBS (Thermo Fisher Scientific, Waltham, MA). Cells were routinely tested (negative ...
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bioRxiv - Microbiology 2023Quote: ... mock and SARS-CoV-2 infected cultures were washed with calcium/magnesium-free PBS and disassociated using TrypLE (Gibco, 5 minutes at 37° C), after which samples were centrifuged (200 x g ...
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bioRxiv - Neuroscience 2024Quote: ... we washed the sections six times (5 min each) and then incubated them for 2 hours in donkey anti-rat-Alexa Flour 594 antiserum (Invitrogen, Product#: A21209, Lot#: 1744721) and biotinylated donkey anti-rabbit secondary antiserum (Jackson ImmunoResearch ...