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Citations for Thermo Fisher :
701 - 750 of 10000+ citations for Human Immunodeficiency Virus Tat Protein HIV 1 Clade C TV019 since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2022Quote: ... HRP (ThermoFisher; 1:5000, 60 min at 4°C). Detection was achieved with Pierce™ ECL Western Blotting Substrate using a ChemDoc XR+ (BioRad).
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bioRxiv - Cell Biology 2022Quote: ... c-Myc (Invitrogen, clone 9E10, MA1-980, 1:1000), V5 (Invitrogen ...
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bioRxiv - Neuroscience 2023Quote: ... incubated overnight at 4° C (MAP2 1:1000 Invitrogen #MA5-12826 ...
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bioRxiv - Neuroscience 2024Quote: ... mouse anti-c-Myc (Invitrogen, 13-2500, 1:1,000) antibodies for overnight at RT ...
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Dual signaling via interferon and DNA damage response elicits entrapment by giant PML nuclear bodiesbioRxiv - Microbiology 2021Quote: ... cells were isolated from human foreskin tissue and cultivated at 37 °C and 5% CO2 in Eagle’s minimal essential medium (Gibco, Thermo Fisher Scientific Inc. ...
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bioRxiv - Microbiology 2021Quote: ... A549 (male, human lung epithelial-like) cells were obtained from ATCC and grown at 37 °C in DMEM (Gibco) supplemented with 10 % FBS (GE Healthcare Life Science ...
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bioRxiv - Molecular Biology 2020Quote: Human HCT116 cells (ATCC) were cultured at 37°C under 5% CO2 in McCoy’s 5A media (Thermo Fisher Scientific) with 1x penicillin/streptomycin and 10% FBS (Invitrogen) ...
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bioRxiv - Microbiology 2020Quote: ... and human umbilical vein endothelial HUV-EC-C cells (ATCC; CRL-1730) in RPMI 1640 medium (Gibco; #11875-093) containing 10% FBS ...
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bioRxiv - Microbiology 2022Quote: Human HEK-293T adherent cells were seeded at 37°C with 5% CO2 in DMEM + GlutaMAX™ (Gibco, Germany) containing 10% heat inactivated fetal calf serum (dFCS ...
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bioRxiv - Neuroscience 2023Quote: Human tissue blocks (N = 6) were stored at −80 °C and transferred to the cryostat (CryoStar NX70, Thermo Scientific) on dry ice ...
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bioRxiv - Genomics 2023Quote: ... human female) cells were cultured in a 37°C 5% CO2 and 20% O2 humidified incubator with DMEM (Gibco) supplemented with 10% heat-inactivated FBS (Gibco) ...
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bioRxiv - Genomics 2023Quote: ... falciparum clone II313 was maintained in human A+ erythrocytes at 37 °C in RPMI 1640 medium containing AlbumaxII (Invitrogen) supplemented with 2 mM L-glutamine ...
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bioRxiv - Immunology 2022Quote: ... Two micrograms of liver RNA per sample was used for qRT-PCR analysis for Plasmodium 18S rRNA using TaqMan Fast Virus 1-Step Master Mix (Applied Biosystems). Data were normalized for input to the GAPDH control (hepatocytes ...
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bioRxiv - Immunology 2021Quote: ... The isolated RNA was then evaluated in RT-qPCR using the TaqMan Fast Virus 1-step Master Mix (Thermo Fisher Scientific) on a QuantStudio Flex 6 Real-Time PCR System (Applied Biosystems ...
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bioRxiv - Microbiology 2021Quote: ... and infected at a MOI of 1 with the VSV-ΔG-Fluc-2A-GFP/VSV-G virus diluted in 10mL Opti-MEM (Life Technologies). The cells were incubated 1 hour at 37C with 5% CO2 ...
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bioRxiv - Microbiology 2020Quote: ZIKV RNA samples were each measured in triplicate on an Applied Biosystems ViiA 7 machine using a Taqman Fast Virus 1-Step MasterMix (Thermo Fisher) with primers ZIKV 1087 forward (CCGCTGCCCAACACAAG) ...
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bioRxiv - Microbiology 2022Quote: Viral RNA in apical secretions was quantified using the TaqMan™ Fast-Virus-1 Step master mix (Thermo Fisher, Basel, Switzerland) with these cycling parameters ...
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bioRxiv - Microbiology 2022Quote: ... HuNoV RNA genome equivalents (GEs) were determined by reverse transcription-quantitative PCR (RT-qPCR) analysis using TaqMan Fast Virus 1-Step Master Mix (Thermo Fisher) and GII specific primer/probe sets (Kageyama et al. ...
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bioRxiv - Bioengineering 2020Quote: ... vRNA levels were determined by quantitative real-time PCR (qRT-PCR) using Applied BiosystemsTM TaqManTM Fast Virus 1-Step Master Mix (Thermofisher #4444432) and a primer-probe combination recognizing a conserved region of gag (GAG5f ...
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bioRxiv - Neuroscience 2020Quote: Total RNA (~40 μg per reaction) was reverse-transcribed and PCR amplified using Taqman Fast Virus 1-Step Master Mix (Applied Biosystems) and primetime assays for specific genes (IDT) ...
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bioRxiv - Microbiology 2021Quote: ... The eluted RNA was combined with Applied Biosystems™ TaqMan™ Fast Virus 1-step master mix (ThermoFisher, Waltham, MA, USA). The target regions for the CSG ...
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bioRxiv - Microbiology 2021Quote: ... 5 μl of the RNA extraction final elution was reverse-transcribed to cDNA and amplified according to the manufacturer’s protocol using TaqMan™ Fast Virus 1-Step Master Mix (ThermoFisher Scientific). The primer pair was as follows ...
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bioRxiv - Microbiology 2022Quote: ... 7 µl total RNA was amplified in a mix containing 5 µl of TaqMan Fast Virus 1-step master mix (Life Technologies), 0.4 µl primer-probe mix for IAV 40 and 9.6 µl distilled water ...
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bioRxiv - Microbiology 2022Quote: Copies of the SARS-CoV-2 N gene (genomic) were measured by qRT-PCR TaqMan Fast Virus 1-step assay (Applied Biosystems). SARS-CoV-2 specific primers and probes from the 2019-nCoV RUO Assay kit (Integrated DNA Technologies ...
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bioRxiv - Microbiology 2022Quote: Copies of SARS-CoV-2 E gene (subgenomic) were measured by qRT-PCR with TaqMan Fast Virus 1-step assay (Thermo Fisher). SARS-CoV-2 specific primers and probes sgLeadSARSCoV2-F 5’-CGATCTCTTGTAGATCTGTTCTC-3’ ...
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bioRxiv - Microbiology 2022Quote: ... 5 µl total RNA was amplified in a mix containing 5 µl of TaqMan Fast Virus 1-step master mix (Life Technologies), 1 µl primer-probe mix for β-actin ...
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bioRxiv - Microbiology 2023Quote: ... The samples were used for quantitating HuNoV RNA GEs by means of reverse transcription-quantitative PCR (RT-qPCR) analysis using a TaqMan Fast Virus 1-Step Master Mix (Thermo Fisher) and GII specific primer/probe sets ...
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bioRxiv - Immunology 2023Quote: ... Both assays were performed using the Taqman Fast Virus 1-Step Master Mix RT-qPCR kit (Applied Biosystems Inc., Carlsbad, CA) on an LC96 instrument (Roche ...
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bioRxiv - Immunology 2023Quote: ... 2μg liver RNA per sample was used for qRT-PCR analysis for Plasmodium 18S rRNA using TaqMan Fast Virus 1-Step Master Mix (Applied Biosystems). Data were normalized for input to the GAPDH control for each sample and are presented as ratios of Plasmodium 18s rRNA to GAPDH RNA signals ...
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bioRxiv - Cancer Biology 2024Quote: ... Subsequent qPCR was performed using the TaqMan Fast Virus 1-Step Master Mix and StepOnePlus Real-Time PCR System (Thermo Fisher). The primers purchased from TaqMan were ...
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bioRxiv - Neuroscience 2020Quote: ... Lysate was centrifuged at 14,000g for 5min at 4°C and protein concentration was measured using Pierce BCA protein assay kit (Thermo Fisher Scientific, MA, USA PI23227) following manufacturer’s instructions ...
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bioRxiv - Physiology 2019Quote: ... The total protein of the kidneys was extracted by centrifugation at 14,000g for 15 minutes at 4°C and protein concentration was determined using micro bicinchoninic acid (BCA) protein assay kit (Thermo Scientific, Rockford, Il, USA) according to manufacturer’s guidelines ...
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bioRxiv - Biochemistry 2022Quote: ... were centrifuged at 16,000 g for 30 minutes at 4 °C and the supernatant was collected for protein estimation using Pierce™ BCA Protein Assay Kit (ThermoFisher Scientific; Cat# 23227). Equal amounts of liver protein (100 μg ...
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bioRxiv - Cancer Biology 2023Quote: ... for 2hours at 4°C and this was followed by precipitation of the protein-antibody complex with dynabeads protein G (Invitrogen, #300D, Carlsbad, CA, USA) for 2h in 4°C on rotator ...
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bioRxiv - Plant Biology 2023Quote: ... The nuclear and cytosolic fractions were boiled in 1X Laemmli buffer (Laemmli, 1970) for 10mins at 100°C and protein was quantified using BCA compatible protein kit (Thermo Fisher Scientific, Whatham, USA). Fifty micrograms of protein were loaded onto a 4-20% gradient SDS-PAGE denaturing protein gel as described above ...
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bioRxiv - Genomics 2024Quote: ... was coupled for 4 hours to 40µl of 1:1 mix Protein G and Protein A Dynabeads (Thermo Scientific) and used for overnight chromatin immunoprecipitation in IP buffer (1% Triton X-100 ...
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bioRxiv - Genetics 2021Quote: ... Epitope-tagged bait proteins were detected with mouse anti-c-myc monoclonal antibody (Invitrogen, MA1-980) and IR-680 conjugated donkey anti-mouse secondary antibody (LI-COR ...
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bioRxiv - Immunology 2021Quote: ... and protein was incubated overnight at 4°C with a Pierce endotoxin removal column (88274, ThermoFisher). Protein was eluted by spin filtration ...
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bioRxiv - Cell Biology 2019Quote: ... The protein lysates were incubated over night at 4 °C with streptavidin agarose beads (Thermo Scientific) to precipitate LecB-biotin-protein complexes ...
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bioRxiv - Immunology 2022Quote: ... for protein expression were maintained at 37°C and 8% CO2 in Expi293 Expression medium (GIBCO), transfected using Expi293 Expression System Kit (GIBCO ...
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bioRxiv - Microbiology 2022Quote: ... The protein with a C-terminal Strep-tag was expressed in Expi293 cells (Thermo Fisher Scientific) and subsequently purified using Strep-Tactin XT Superflow high capacity resin (IBA Lifesciences ...
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bioRxiv - Cancer Biology 2019Quote: ... followed by 3–4-hour incubation at 4°C with protein A/G agarose (20421, Invitrogen). The beads were then collected for western blot detection ...
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bioRxiv - Molecular Biology 2020Quote: ... 4°C and supernatant protein concentration was quantified with Enhanced BCA protocol (Thermo Fisher Scientific, Pierce). Equivalent amounts of proteins were separated on an SDS-page (approximately 30 µg ...
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bioRxiv - Plant Biology 2019Quote: Microsomal proteins were predigested for three hours with endoproteinase Lys-C (0.5 µg/µl; Thermo Scientific) at 37 °C in 6 M urea 2 M thiourea ...
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bioRxiv - Physiology 2021Quote: ... The reactions were then incubated for 1h at 4°C with protein A/G dynabeads (Invitrogen) pre-blocked in PBS/0.5% BSA/0.5% Tween ...
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bioRxiv - Microbiology 2020Quote: ... Bound protein was detected via the C-terminal 6XHis Tag using 6XHis Antibody MA1-135 (Invitrogen) followed by three washes in TBS-T and incubation with donkey anti-mouse horseradish peroxidase (HRP)-coupled secondary antibody (Dianova) ...
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bioRxiv - Biochemistry 2021Quote: ... followed by protein precipitation with 300 µL of cold (−20°C) acetonitrile (Thermo Fisher, Waltham, MA). The sample was vortexed again for 30 s and chilled for 30 min at −20°C ...
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bioRxiv - Cell Biology 2022Quote: ... for 2 hours at 4°C and incubated with 40ul washed Dynabeads Protein G (Invitrogen 10003D) for 1 hour ...
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bioRxiv - Immunology 2022Quote: The recombinant proteins were purified using a CaptureSelect™ C-tagXL Affinity Matrix prepacked column (ThermoFisher) and followed the manufacturer’s guideline 66 ...
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bioRxiv - Physiology 2023Quote: ... 0.5 mg protein was precleared for 30 minutes at 4°C with 4% Agarose beads (ThermoFisher). To immunoprecipitate RYR1 ...