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Citations for Thermo Fisher :
651 - 700 of 10000+ citations for Human Immunodeficiency Virus Tat Protein HIV 1 Clade C TV019 since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2019Quote: ... and 5 µg of human Cot-1 DNA (ThermoFisher Scientific) and then denatured in deionized formamide (Merck ...
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bioRxiv - Bioengineering 2021Quote: ... 1 ng/mL human FGF-2 (ThermoFisher #68-8785-63), and 1% Primocin (Invivogen #ant-pm-1 ...
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bioRxiv - Immunology 2022Quote: ... anti-Human CD279(PD-1) antibody (Invitrogen, eBioscience, PE-Cyanine7), anti-Human CD223(LAG-3 ...
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bioRxiv - Immunology 2021Quote: ... 1:100 mouse anti-human CXCR5 PE-Cy7 (MU5UBEE, ThermoFisher) was added to each well at the start of stimulation ...
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bioRxiv - Neuroscience 2020Quote: ... anti-human PHF-tauThr231 (1:1000; AT180, Thermo Scientific, USA). The secondary antibodies used against above were anti-Mouse Cy3 (1:200) ...
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bioRxiv - Neuroscience 2020Quote: ... anti-human PHF-tauThr231 (AT180; 1:1000, Thermo Scientific, USA), PHF-tauThr181 (AT270 ...
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bioRxiv - Microbiology 2022Quote: ... 0.3 μg of recombinant human CDKL5 (1-498) (ThermoFisher, A33353) was combined with 3 μg of recombinant human SQSTM1/p62 protein (GeneTex ...
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bioRxiv - Immunology 2019Quote: ... ProcartaPlex Human Cytokine/Chemokine/Growth Factor Panel 1 (Thermo Scientific), as previously described [14] ...
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bioRxiv - Bioengineering 2019Quote: ... then with human-CD68 (FITC conjugated 1:20 dilution; Invitrogen), following manufacturer’s protocol ...
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bioRxiv - Immunology 2021Quote: ... rabbit anti-human GRZMK (Invitrogen, LS-C119554-50, 1:100), rabbit anti-human E-cadherin (Abcam ...
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bioRxiv - Bioengineering 2022Quote: ... 1 ng/mL human FGF-2 (ThermoFisher #68-8785-63), and 1% antibiotic-antimycotic (ThermoFisher #1540062) ...
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bioRxiv - Microbiology 2022Quote: ... 1:5000 dilution rabbit anti-human Lcn2 (Invitrogen, Cat. 702248), 1:1000 dilution rabbit anti-human peptidylarginine deiminases 4 (PAD4 ...
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bioRxiv - Neuroscience 2022Quote: ... Rat anti-human CD44 (1:500, ThermoFisher, 14-0441-82), Rabbit anti-human GFAP (1:400 ...
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bioRxiv - Evolutionary Biology 2023Quote: ... goat anti-human Alexa Fluor 546 (1:400, Invitrogen Corporation) and goat anti-mouse Alexa Fluor 555 (1:200 ...
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bioRxiv - Cancer Biology 2023Quote: ... and rat anti-human CD44 (1:50, clone IM7, Invitrogen). The secondary antibodies included alexa fluor 488 labelled donkey anti-mouse (xxx ...
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Critical contribution of mitochondria in the development of cardiomyopathy linked to desmin mutationbioRxiv - Pathology 2023Quote: ... Gibco Human Episomal iPSC clone (Control 1, Thermo Fisher Scientific) and CW30318 (Control 2 ...
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bioRxiv - Cell Biology 2023Quote: ... followed by anti-human IgG–Alexa594 (1:1000, ThermoFisher Scientific) for 30 min and analyzed by flow cytometry ...
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bioRxiv - Developmental Biology 2024Quote: ... 20 μg of human Cot-1 DNA (Invitrogen Cat# 15279011), 20 μg of poly(A ...
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bioRxiv - Cell Biology 2021Quote: ... monoclonal mouse anti-CRABP1 (1:500, MA3-813, C-1, ThermoFisher, USA) was labeled with Alexa Fluor 488 tyramide solution ...
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bioRxiv - Molecular Biology 2023Quote: ... for 1 hour at 37°C with 1 μl RNaseOUT (10777019, Invitrogen), then purified by acid phenol extraction at 65°C for 45 min in a thermomixer with shaking at 1400 RPM ...
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bioRxiv - Genomics 2020Quote: ... SDS) for 10 minutes at 95°C and protein concentration was determined using the Pierce™ BCA Protein Assay Kit (ThermoFisher Scientific 23227). Protein samples were separated by SDS-PAGE using 4-20% Mini-PROTEAN® TGX™ gels (BioRad ...
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bioRxiv - Genetics 2020Quote: ... This mixture was incubated overnight at 4°C with beads (Protein A Dynabeads, Invitrogen, for CTCF ChIP; Protein G Dynabeads, Invitrogen, for H3K9me3 ChIP) that had been pre-blocked with 0.5% BSA and mixed with polyclonal CTCF antibody (C15410210-50 ...
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bioRxiv - Genomics 2022Quote: ... for 30 minutes at 4°C and supernatant was retained and protein concentration quantified using the Pierce BCA protein assay kit (Thermo Fisher Scientific, 23227). A total of 25-35μg proteins were loaded onto 12.5% Tris-HCl gel for SDS-PAGE analysis ...
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bioRxiv - Neuroscience 2023Quote: ... Homogenized samples were centrifuged at 14,000 rpm for 15 min at 4°C and the protein concentration of the supernatant was determined by Pierce BCA Protein Assay (ThermoFisher Scientific, cat# 23227). Samples were diluted and supplemented with SDS sample buffer (50 mM Tris-HCl pH 6.8 ...
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bioRxiv - Cell Biology 2023Quote: ... Samples were centrifuged for 10 min at 10,000 times gravity (ξ g) at 4 °C insoluble materials and total protein concentration was measured by the PierceTM BCA protein assay (Thermo Fisher Scientific). 15-20 µg of samples was prepared in Laemmli buffer and run in 8% SDS-PAGE before being transferred onto nitrocellulose membrane at 320 mA for 2.5 h ...
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bioRxiv - Cell Biology 2022Quote: ... The lysates were cleared by centrifugation at 18,000 x g and 4°C for 20 min and protein concentration was determined using the BCA Protein Assay Kit from Pierce™ (Thermo Fisher Scientific) according to the manufacturer’s protocol ...
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bioRxiv - Microbiology 2020Quote: ... vif vpr and vpx coding sequences were amplified with specific primers from pKP59-HIV-2 ROD plasmid and cloned into pcDNA3.1 vector (Invitrogen). Expression vectors encoding the six different mutants of HIV-2 Env cytoplasmic tail were created by introducing stop codon in different position within the cytoplasmic tail by site-directed mutagenesis using the QuickChange II Site-Directed Mutagenesis Kit (Agilent) ...
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bioRxiv - Cancer Biology 2022Quote: ... Dilute 2.5μg of lentiviral expression plasmid and 2.5 μg of Lenti-Pac HIV mix into 200μl of Opti-MEM® I (Invitrogen). In a separate tube diluting 15 μl of EndoFectin Lenti into 200μl of Opti-MEM I ...
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bioRxiv - Neuroscience 2023Quote: ... and 1.3 nM human insulin-like growth factor 1 (hIGF-1, Thermo Fisher Scientific). The growth media were changed after 24 h and further regularly changed every 4 days ...
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bioRxiv - Biochemistry 2022Quote: ... 1 μL pre-washed magnetic-conjugated protein G beads (Dynabeads protein G, Invitrogen) were added to the reactions followed by the incubation for 1 hour at 4°C in the thermomixer (750 rpm) ...
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bioRxiv - Microbiology 2021Quote: ... followed by 30 minutes with 0.1% Tween/PBS at 37°C and 1 hour at 37°C with secondary antibody Alexa-488 goat anti-mouse (1:500) (Life Technologies #A11029) in 1% BSA/PBS before mounting with Prolong Gold with DAPI (Invitrogen #P36935) ...
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bioRxiv - Microbiology 2021Quote: ... virus stocks were prepared in serum-free VP-SF media (Invitrogen). Viruses were harvested before CPE development ...
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bioRxiv - Cell Biology 2021Quote: ... The virus packaging was performed in HEK293FT cells (Thermo Fisher Scientific) based on a calcium precipitation method using pUMVC and pCMV-VSV-G vectors (37 ...
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bioRxiv - Immunology 2021Quote: ... Virus particles in 2% FBS/DMEM/1mM Pen/Strep (Thermofisher, 15140122) were incubated with cells for 2 hours at room temperature ...
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bioRxiv - Microbiology 2021Quote: ... cells and purified virus particles were lysed with RIPA (ThermoFisher Scientific) buffer supplemented with complete protease inhibitor cocktail (Roche ...
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bioRxiv - Microbiology 2020Quote: ... Virus stocks were treated with 40 U/mL DNase I (Invitrogen) for 1 hr at 37°C before they were used to infect cells for 2 hr at an MOI of 0.5-2 ...
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bioRxiv - Physiology 2022Quote: ... and 200 U of Moloney murine leukemia virus reverse transcriptase (Invitrogen) in a final reaction volume of 20 μL (Evans-Molina et al. ...
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bioRxiv - Genetics 2020Quote: ... Cells were then transduced using CytoTune-iPS 2.0 Sendai virus (Invitrogen) as outlined in the supplier’s protocol at an MOI of 5:5:3 (KOS [Klf4 ...
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bioRxiv - Microbiology 2021Quote: Virus replication assays were performed in RPMI 1640 (Gibco 11875-093) supplemented with 10% FBS ...
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bioRxiv - Biochemistry 2023Quote: ... virus inoculum was exchanged with serum-free MEM (Gibco, Life Technologies) containing 0.75% carboxymethylcellulose (Sigma-Aldrich ...
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bioRxiv - Biochemistry 2023Quote: ... virus inoculum was exchanged with serum-free MEM (Gibco, Life Technologies) containing 0.75% carboxymethylcellulose (Sigma-Aldrich ...
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bioRxiv - Microbiology 2023Quote: ... dNTP and Moloney murine leukemia virus (M-MLV) reverse transcriptase (Invitrogen) were used to synthesize cDNA ...
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Neuronal L-Type Calcium Channel Signaling to the Nucleus Requires a Novel CaMKIIα-Shank3 InteractionbioRxiv - Neuroscience 2019Quote: ... and rocked end-over-end at 4°C for 1 hour with 10 μl prewashed Dynabeads Protein A (Thermo Fisher Scientific, Cat. #10001D, for rabbit) or Protein G (Thermo Fisher Scientific ...
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bioRxiv - Immunology 2021Quote: ... Protein secretion levels in culture supernatant were quantified via ELISA for human IgG (Invitrogen, 50-112-8849) and human BAFF (R&D Systems ...
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bioRxiv - Microbiology 2022Quote: Recombinant human CD81 protein (R &D Systems) was coated (4µg/ml) on ELISA plate (Thermo Fisher Scientific) and incubated overnight at 40C ...
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bioRxiv - Cell Biology 2021Quote: Peak lists were searched against the complete human proteome (UniProt release 2014_08, with 68.049 proteins) using SEQUEST (Proteome Discoverer 1.3, ThermoFisher) as search engine ...
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bioRxiv - Cell Biology 2020Quote: ... Cytochrome c oxidase subunit 1 (COX1; 459600) from Invitrogen; Cytochrome c oxidase subunit 2 (COX2 ...
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bioRxiv - Cancer Biology 2019Quote: ... 5 μL of Streptavidin C-1 beads (Thermo Fisher) were washed with Tween Wash Buffer (5 mM Tris-HCl pH 7.5 ...
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bioRxiv - Molecular Biology 2019Quote: ... 5 µL of Streptavidin C-1 beads (Thermo Fisher) were washed with Tween Wash Buffer (5 mM Tris-HCl pH 7.5 ...
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bioRxiv - Systems Biology 2021Quote: ... Frost® Cryo 1°C Freezing Container (ThermoFisher scientific) with Isopropyl alcohol at −80°C over-night ...