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Citations for Thermo Fisher :
901 - 950 of 10000+ citations for Human Immunodeficiency Virus Tat Protein HIV 1 Clade C TV019 since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Systems Biology 2023Quote: ... 1 mL of Protein G Dynabeads (ThermoFisher, #10003D) were washed once with 1X PBST (1X PBS + 0.1% Tween-20 ...
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bioRxiv - Genomics 2023Quote: 1 mL of Protein G Dynabeads (ThermoFisher, #10003D) were washed once with 1X PBSt (1X PBS + 0.1% Tween-20) ...
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bioRxiv - Developmental Biology 2024Quote: ... 1 ng TrueCut Cas9 Protein v2 (Invitrogen, A36497), and 125 pg myc-GFP mRNA ...
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bioRxiv - Cancer Biology 2021Quote: ... anti-c-Jun (Thr239) (PA5-104748, dilution 1:1000, Invitrogen) and anti-p-SMAD3 (S423/425 ...
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bioRxiv - Developmental Biology 2020Quote: ... anti-c-Myc (9E10) (1:2000, MA1-980, ThermoFisher Scientific), goat anti-mouse IgG ...
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bioRxiv - Immunology 2021Quote: ... anti-phospho-c-Jun(Ser 73) (Thermofisher; dilution 1:100), anti-LAMP1 (Proteintech ...
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bioRxiv - Genomics 2019Quote: ... 1 μl of ERCC spike-in (c = 0.01x; Thermo Fisher) per library was included ...
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bioRxiv - Cancer Biology 2020Quote: ... and Anti c-MYC (1:1000, 9E10, Thermo Fisher Scientific) antibodies were used to detect proteins.
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bioRxiv - Cell Biology 2021Quote: ... mouse HMB45 (1:20; 4°C overnight; Life Technologies, 081050). Afterwards ...
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bioRxiv - Neuroscience 2022Quote: ... for 2 days at 4 °C (Invitrogen, both 1:500). After washing ...
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bioRxiv - Neuroscience 2023Quote: ... 1 µl of ERCC spike-in (c = 0.01×; Thermo Fisher) per library was included ...
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bioRxiv - Neuroscience 2023Quote: ... and processed at 4°C with NeuroTrace (1:500, Invitrogen) in PBS containing 0.3% Triton-X ...
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bioRxiv - Neuroscience 2023Quote: ... holding stage of 60°C for 1 minute (Applied Biosystems 7500 Fast Real-Time PCR System ...
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bioRxiv - Cancer Biology 2024Quote: ... for 1 hour at 37°C followed by TryLE (Gibco) digestion ...
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bioRxiv - Genomics 2024Quote: ... 5 μL of Streptavidin C-1 beads (Thermo Fisher # 65001) was used to capture the Biotin Datp labeled DNA ...
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bioRxiv - Cell Biology 2019Quote: ... ELISAs were performed using FBLN5 (Fibulin-5) Human ELISA Kit from Fine Test (EH0772) and Thrombospondin 1 (TSP1) Human ELISA Kit from Invitrogen (BMS2100), following the manufacturer’s protocols ...
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bioRxiv - Genomics 2019Quote: ... Proteins (20µg) were heated at 95°C with β-mercaptoethanol and then separated by SDS PAGE (Invitrogen). Nitrocellulose membranes were blocked in 5% nonfat milk in PBST for 1 hr and then incubated in primary antibody overnight at 4ºC ...
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bioRxiv - Biochemistry 2019Quote: ... 4°C and lysed using 400 µL B-PER bacterial protein extraction reagent (Thermo Scientific, MA, USA) according to the instructions of the manufacturer ...
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bioRxiv - Neuroscience 2020Quote: ... and then incubated at 4°C for 30-60 minutes with Protein A Dynabeads (Thermo Fisher Scientific). The beads were washed five times in wash buffer (20 mM Tris ...
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bioRxiv - Neuroscience 2020Quote: ... 4 °C) and the protein concentration of the supernatant was determined using a BCA assay (ThermoFisher 23225). Samples were denatured (70% sample ...
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bioRxiv - Microbiology 2022Quote: ... Protein lysates were boiled at 95 °C for 5 mins in 6x SDS sample buffer (Thermo Scientific). Proteins were separated on 4-12% Bis-Tris SDS-PAGE gels (Thermo Fisher ...
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bioRxiv - Developmental Biology 2022Quote: ... at 95°C for 5 min and separated using NuPAGE 4%–12% Bis-Tris Protein Gel (Invitrogen) in NuPAGE MES SDS Running Buffer (Invitrogen) ...
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bioRxiv - Molecular Biology 2020Quote: ... The protein was denatured at 70 °C for 10 min with LDS Sample Buffer (Invitrogen, Cat#: NP0007) and Sample Reducing Agent (Invitrogen ...
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bioRxiv - Microbiology 2020Quote: The purified JEV structural proteins (C, M, E, ED III) from the S2 insect expression system (Invitrogen) were quantified by using the bicinchoninic acid (BCA ...
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bioRxiv - Biochemistry 2021Quote: ... 0.03% NP40) and proteins were eluted by incubation in 1x LDS-sample buffer (Thermofisher, c/n NP0007) at 65°C for 10 minutes ...
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bioRxiv - Microbiology 2021Quote: ... sheared chromatin was precleared for 20 min at 4’C using Protein G Dynabeads (Thermo Fisher Scientific). Immunoprecipitations were performed using fresh beads and anti-Histone H3 acetyl K27 (H3K27Ac ...
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bioRxiv - Molecular Biology 2022Quote: ... and stored at −30°C before separation on 4-12% NuPAGE Bis-Tris protein gels (ThermoFisher Scientific).
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bioRxiv - Immunology 2022Quote: Recombinant tri-S protein was heat-denatured at 100°C for 3 min in loading buffer (Invitrogen) containing 1X sample reducing agent (Invitrogen) ...
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bioRxiv - Molecular Biology 2019Quote: ... Immunoprecipitation was performed at 4°C for 3 hr with 50 µl protein G dynabeads (Thermo Fisher). Beads were washed five times with ice-cold Wash buffer 1 (50 mM Hepes-KOH (pH7.6) ...
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bioRxiv - Microbiology 2020Quote: Proteins with C-terminal biotin tags were attached to streptavidin-coated dynabeads (Dynabead M-280 Streptavidin, Invitrogen) following standard procedures ...
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bioRxiv - Genetics 2020Quote: ... Proteins were denatured for 10 min at 70°C in NuPAGE LDS Sample Buffer (4X) (Invitrogen; NP0007) and NuPAGE Sample Reducing Agent (10X ...
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bioRxiv - Physiology 2021Quote: ... The total protein was inoculated with anti-HA magnetic beads at 4 C (Thermo Scientific, Rockford, IL) overnight ...
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bioRxiv - Immunology 2022Quote: ... STAT1 was immunoprecipitated by incubating for five hours at 4°C with Protein-G DynaBeads (Invitrogen #10004D). DNA bound to STAT1 complexes was extracted using 1% SDS and 0.6 mg/ml of Proteinase K (New England Biolabs #P81075 ...
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bioRxiv - Microbiology 2023Quote: ... Protein samples were denatured at 90 °C for 10 min with 10× reducing agent (Thermo Fisher Scientific) before gel electrophoresis ...
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bioRxiv - Biophysics 2024Quote: ... precleared and immunoprecipitated overnight at 4 °C with 50 μL of GFP-conjugated Dynabeads Protein A (Invitrogen). Beads had previously been conjugated with 5 μL anti-GFP antibody (Invitrogen ...
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bioRxiv - Immunology 2023Quote: ... sheared chromatin was precleared for 20 min at 4°C using Protein G Dynabeads (Thermo Fisher Scientific). Immunoprecipitations were performed using fresh beads and anti-Histone H3 acetyl K9 (H3K9Ac ...
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bioRxiv - Microbiology 2023Quote: ... before being incubated overnight at 4 degrees C with the antibodies bound to Protein A Dynabeads (Invitrogen). Samples were washed for 3 minutes each at 4 degrees C with low salt wash (0.01% SDS ...
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bioRxiv - Biochemistry 2022Quote: ... Target protein was coated overnight (O/N) at 4 °C on MaxiSorp™ 96-well plates (Nunc). ELISA-blocking buffer (PBS containing 0.5% (w/v ...
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bioRxiv - Developmental Biology 2023Quote: ... at 95°C for 5 min and separated using NuPAGE 4%–12% Bis-Tris Protein Gel (Invitrogen) in NuPAGE MES SDS Running Buffer (Invitrogen) ...
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bioRxiv - Plant Biology 2023Quote: ... The protein samples were denatured at 65 °C in the 1X LDS sample buffer (Invitrogen, Waltham, MA) with 2.5% β-mercaptoethanol for 5 minutes ...
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bioRxiv - Biophysics 2024Quote: ... precleared and immunoprecipitated overnight at 4 °C with 50 μL of GFP-conjugated Dynabeads Protein A (Invitrogen). Beads had previously been conjugated with 10 μL anti-GFP antibody (Invitrogen ...
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bioRxiv - Neuroscience 2024Quote: ... 4°C at 20817 x g and protein concentrations were determined by BCA assay (Thermo Fisher, 23225). Cell lysates (10 μg for HEK293E cells or 15 μg for MEFs ...
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bioRxiv - Biochemistry 2024Quote: ... 4°C) and the protein concentration determined by a BCA assay (Pierce BCA Kit, Thermo Fisher Scientific). The human protein extracts were further processed to peptides in the same manner as described above for bacterial protein extracts.
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bioRxiv - Biochemistry 2024Quote: ... The obtained supernatants (cell lysates) were incubated at 4°C with Dynabeads Protein G (Thermo Fisher Scientific) pre-bound with antibodies ...
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bioRxiv - Microbiology 2023Quote: ... xanthus was grown at 32°C in 1% CTT broth (1% Bacto Casitone (Gibco), 10mM Tris-HCl pH 8.0 ...
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bioRxiv - Cell Biology 2021Quote: ... Protein extracts were then incubated for 1 hour with protein A-conjugated Dynabeads (Life Technologies), after which the beads were washed with lysis buffer at incremental KOAc concentrations (100 mM ...
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bioRxiv - Microbiology 2021Quote: ... 1 µl was used for protein quantification with the Pierce BCA Protein Assay Kit (Thermofisher). The remaining 9 µl were mixed with SDS (0.2% final) ...
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bioRxiv - Cancer Biology 2021Quote: ... the mixture of 2.5 μg of each lentiviral ORF expression plasmid and 0.25 μg of the Lenti-Pac HIV plasmid in 15 μL of EndoFectin Lenti was incubated with 200 μL of Opti-MEM® (Invitrogen). The DNA-EndoFectin Lenti complex was directly added into cultured cells before being allowed for overnight incubation at 37°C in a CO2 incubator ...
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bioRxiv - Cancer Biology 2021Quote: ... Lentiviral vector CSII-CMV-MCS-IRES2-Bsd harboring a cDNA was transfected with the packaging vectors pCAG-HIV-gp and pCMV-VSV-G-RSV-Rev into X293T cells using Lipofectamine2000 transfection reagent (Life Technologies/Thermo Fisher Scientific ...
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bioRxiv - Molecular Biology 2021Quote: KBM7 (a kind gift from Thijn Brummelkamp) and Hap-Lat (latent HIV infected KBM7-derived cell lines) were cultured in IMDM media (ThermoFisher Scientifc) supplemented with 10% FCS and 2% Pen/Strep ...