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Citations for Thermo Fisher :
601 - 650 of 10000+ citations for Human Immunodeficiency Virus Tat Protein HIV 1 Clade C TV019 since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2020Quote: ... followed by a 1.5 hrs protein-chromatin pull-down with a 1:1 mix of protein A and protein G Dynabeads (Thermo Fisher; 10001D and 10003D). ChIP samples were washed extensively ...
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bioRxiv - Bioengineering 2022Quote: ... Anti-human IL6 Ab concentrations were quantified using the Quant-it protein assay kit (Life Technologies). Ab conjugates were validated on 10% TBE urea denaturing gels and validated on an agarose gel (Supplementary Figure S8) ...
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bioRxiv - Immunology 2022Quote: ... The secreted fusion protein was purified on a CaptureSelect™ human albumin affinity matrix (Life Technologies), and protein eluted by adding 20 mM Tris and 2.0 M MgCl2 ...
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bioRxiv - Genomics 2022Quote: ... human preadipocytes in a 6-well plate were transfected with 6.25ug Cas9 protein (Fisher Scientific, A36498) and 800ng sgRNAs (IDT) ...
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bioRxiv - Cell Biology 2021Quote: ... 5 μCi γ32P-ATP and 350 ng CDK1/Cyclin B Recombinant Human Protein (Thermo Fisher PV3292) in kinase buffer (50 mM HEPES pH 7.5 ...
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bioRxiv - Microbiology 2023Quote: Human ACE2 fusion proteins were obtained from transient transfection using the Expi293TM system (Thermo Fisher Scientific). All plasmids used in this study were generated by ATUM Bio and transient transfection was carried out following the manufacturer’s protocol for the ExpiFectamineTM 293 transfection kit (Thermo Fisher Scientific) ...
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bioRxiv - Immunology 2019Quote: ... Protein A and Protein G coupled magnetic beads (mixture 1:1, Dynabeads, Thermo Fisher, 10002D and 10004D) were pretreated with BSA (NEB ...
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bioRxiv - Molecular Biology 2022Quote: ... Complexes were captured with a 1:1 mixture of magnetic Protein A and Protein G Dynabeads (Invitrogen) for 3 h at 4°C ...
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Enhancer Remodeling Promotes Tumor-Initiating Activity in NRF2-Activated Non-Small Cell Lung CancersbioRxiv - Cancer Biology 2020Quote: ... were crosslinked to a 1:1 mixture of Dynabeads protein A and protein G (Thermo Fisher Scientific) with DMP and incubated with the supernatant at 4°C for 2 hr ...
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bioRxiv - Bioengineering 2022Quote: ... A 40 μL aliquot of a 1:1 mixture of Protein A and Protein G Dynabeads (Invitrogen) was added and incubated for an additional 4 h at 4°C on a rotator (400 rpm) ...
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bioRxiv - Immunology 2023Quote: ... A 20 μL aliquot of a 1:1 mixture of Protein A and Protein G Dynabeads (Invitrogen) was added and incubated for 4 h at 4°C with rotation (50 rpm) ...
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bioRxiv - Developmental Biology 2021Quote: ... where EGF and noggin were replaced with human forms with 10 ng ml−1 human EGF (Invitrogen, PHG0311), 100 ng ml−1 human noggin (Peprotech ...
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bioRxiv - Immunology 2020Quote: ... mouse anti-human IgG3 (HP6050) or mouse anti-human IgM (clone HP6083) at 1/1000 (Thermo Fisher Scientific) for 1 hour at RT ...
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bioRxiv - Microbiology 2021Quote: ... Both human and simian hepatocytes were maintained at 37 °C in 5% CO2 in William’s E medium (Gibco) supplemented with 10% fetal clone III serum (FCS ...
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bioRxiv - Bioengineering 2020Quote: The human fetal osteoblast (hOB) 1.19 cell line (ATCC) was expanded at 33.5°C in DMEM/F12 media (Gibco) containing 10% FBS (Invitrogen ...
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bioRxiv - Cell Biology 2022Quote: ... at 37 °C and 5% CO2 in EMEM (ATCC) supplemented with 0.01 mg/mL human recombinant insulin (Gibco) and 10% (vol/vol ...
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bioRxiv - Cancer Biology 2020Quote: ... and human embryonic kidney epithelial cell line (HEK293T) were maintained at 37°C in RPMI 1640 (Life Technologies) and DMEM supplemented with 10% fetal bovine serum and Penicillin and Streptomycin (1mM) ...
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bioRxiv - Biophysics 2022Quote: ... designated as ‘Cav1.2(ΔC)’) construct was obtained from a full-length human Cav1.2 α1 (Uniprot Q13936-20) template in pcDNA3.1 (Invitrogen) using a Gibson Assembly kit (NEB) ...
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bioRxiv - Microbiology 2023Quote: Human 293F cells were maintained at 37°C with 5-8% CO2 in FreeStyle 293 Expression Medium (ThermoFisher) supplemented with penicillin and streptomycin ...
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bioRxiv - Bioengineering 2023Quote: ... then incubated for 1 hour at 37°C followed by proteinase K digestion (1 μL - 56°C for 10 minutes; ThermoFisher, #EO0491). Products were resolved on a 3% agarose gel stained with SYBR Gold and analyzed using ImageJ.
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bioRxiv - Biochemistry 2021Quote: ... 4°C and the protein was purified from the supernatant using Ni-NTA Agarose (Invitrogen). The beads were washed with His-SUMO buffer and protein was eluted with His-SUMO elution buffer (50 mM Tris-HCl pH 7.5 ...
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bioRxiv - Plant Biology 2021Quote: ... Chromatin samples were incubated for 12 h at 4 °C with Protein A Dynabeads (Invitrogen) precoated with the corresponding antibodies ...
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bioRxiv - Cell Biology 2021Quote: ... endogenous c-MET was immunoprecipitated on MET antibody-bound beads (Protein G from Thermo Fisher) and Flag-tagged HGF WT and HGF 4Cys-4Ala were in-vitro translated (TNT quick coupled Transcription/Translation system ...
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bioRxiv - Immunology 2020Quote: ... 000 x g at 4°C and protein concentration measured using Pierce BCA (Thermo Fisher). Equivalent amount of protein in each sample were immunoprecipitated by rotating with 0.25-1 µg of FADD antibody (M19 ...
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bioRxiv - Neuroscience 2019Quote: ... 500 µg of proteins were used for c-Myc-tagged tomosyn co-immunoprecipitation (Thermo Fisher Scientific ...
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bioRxiv - Microbiology 2020Quote: ... For IP of hnRNP-C and RALY 50 μl of Protein G Dynabeads (Thermo Fisher) per sample were washed 3x in IP buffer (50 mM HEPES pH 7.4 ...
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bioRxiv - Plant Biology 2023Quote: ... The eluted protein was then immobilized on anti-c-Myc magnetic beads (Thermo Fisher 88843) and washed three times with 100 μl IP buffer to isolate homogeneous SEP3Δtet3M-AG or SEP3Δtet-AGΔtet complexes ...
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bioRxiv - Molecular Biology 2023Quote: ... protein samples were denatured at 95°C using NuPAGE LDS sample buffer (Thermo Fisher Scientific) and separated by 4-12% sodium dodecyl sulfate-polyacrylamide gel electrophoresis ...
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bioRxiv - Plant Biology 2023Quote: ... Chromatin samples were incubated for 12 h at 4 °C with Protein G Dynabeads (Invitrogen) precoated with the antibodies anti-FLAG (Abcam ab18230 ...
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bioRxiv - Immunology 2023Quote: ... the recombinant proteins were purified using a CaptureSelectTM C-tagXL Affinity Matrix prepacked column (ThermoFisher), followed the manufacturer’s guidelines as previously detailed (56 ...
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bioRxiv - Immunology 2024Quote: The recombinant proteins were purified using a CaptureSelectTM C-tagXL Affinity Matrix prepacked column (ThermoFisher), followed the manufacturer’s guidelines as reported previously (25 ...
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bioRxiv - Plant Biology 2024Quote: ... Chromatin samples were incubated for 12 h at 4 °C with Protein G Dynabeads (Invitrogen) precoated with the antibodies anti-FLAG (Abcam ab18230 ...
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bioRxiv - Neuroscience 2019Quote: ... 5µl each from the standards and the rAAV dilutions along with 15µl of mastermix were subjected to qPCR at 95°C 10 min / 95°C 15 sec / 60°C 1 min/ repeat 40×/ melt curve using StepOne machine (Applied Biosystems, USA). Data analyses were performed by StepOne2.3 software and by Microsoft excel.
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bioRxiv - Cancer Biology 2021Quote: ... Reverse transcription and quantitative PCR (RT-qPCR) was performed using 0.25 µl of lysate and Taqman Fast Virus 1-step Master Mix (Applied Biosystems) on a CFX384 thermal cycler (Bio-Rad) ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: Copies of SARS-CoV-2 E gene were measured by qRT-PCR TaqMan Fast Virus 1-step assay (Thermo Fisher). SARS-CoV-2 specific primers and probes from the 2019-nCoV RUO Assay kit (Integrated DNA Technologies ...
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bioRxiv - Microbiology 2021Quote: ... 1μL of each extracted RNA was used to performed one step RT-qPCR using TaqMan Fast Virus 1-Step Master Mix (Invitrogen). The relative quantities of envelope (E ...
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bioRxiv - Microbiology 2020Quote: ... qRT-PCR was performed using 100ng total RNA compared to an RNA standard curve using TaqMan Fast Virus 1-Step Master Mix (ThermoFisher) on a Quant Studio 3 (Applied Biosystems).
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bioRxiv - Microbiology 2020Quote: ... Viral RNA was quantified by RT-qPCR using the TaqMan(tm) Fast Virus 1-Step Master Mix (Thermo Fisher Scientific). Primers and probes were based on (21 ...
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bioRxiv - Cell Biology 2021Quote: ... Viral RNA was quantified by RT-qPCR using the TaqMan™ Fast Virus 1-Step Master Mix (Thermo Fisher Scientific). Primers and probes were used as described previously (45) ...
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bioRxiv - Immunology 2022Quote: ... The extracted RNA was then added to TaqMan™ Fast Virus 1-Step Master Mix (Thermo Fisher Scientific, Waltham, MA) containing primers and a fluorescently labeled hydrolysis probe specific for mRNA from the nucleocapsid gene of SARS-CoV-2 (forward primer 5’-CGATCTCTTGTAGATCTGTTCTC-3’ ...
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bioRxiv - Microbiology 2022Quote: ... SARS-CoV-2 RNA was detected with the CDC assay kit (IDT) and the TaqMan Fast Virus 1-Step Master Mix (ThermoFisher) on the QuantStudio3 System (Invitrogen).
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bioRxiv - Microbiology 2022Quote: ... The nCoV_IP2 primer-probe set was used in combination with TaqMan fast virus 1-step master mix (Thermo Fisher Scientific) to detect viral RNA ...
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bioRxiv - Microbiology 2022Quote: ... 1μL of each extracted RNA was used to performed one step RT-qPCR using TaqMan Fast Virus 1-Step Master Mix (Invitrogen). The relative quantities of envelope (E ...
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bioRxiv - Microbiology 2021Quote: ... RT-qPCR was performed as previously described(42) using TaqMan Fast Virus 1-Step Master Mix (Thermo Fisher, Cat#4444436) and a OneStepPlus Real-Time PCR System (96-well format ...
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bioRxiv - Microbiology 2021Quote: ... qRT-PCR was performed as previously described [39] using TaqMan Fast Virus 1-Step Master Mix (Thermo Fisher, Cat#4444436) and a OneStepPlus Real-Time PCR System (96-well format ...
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bioRxiv - Immunology 2020Quote: ... 1μL of each extracted RNA was used to performed one step RT-qPCR using TaqMan Fast Virus 1-Step Master Mix (Invitrogen). The relative quantities of nucleocapsid (N ...
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bioRxiv - Microbiology 2022Quote: Blood virus mixtures were titrated by ten-fold serial dilution in virus media (RPMI 1640 cell culture media supplemented with 5% fetal bovine serum [FBS] and 1% Penicillin-Streptomycin [Gibco]) in a 96-well plate before transferring dilutions to equivalent wells of a 96 well plate containing near confluent C6/36 cell monolayers ...
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bioRxiv - Microbiology 2022Quote: ... The RT-qPCR was performed according to the manufacturer’s protocol using TaqMan Fast Virus 1-Step Master Mix (Thermo Fisher). Fluorescent signals were acquired using QuantStudio 6 Real-time PCR system (Thermo Fisher).
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bioRxiv - Plant Biology 2023Quote: ... RT-PCR was performed with 1 µg of RNA using 100 units of Moloney murine leukemia virus reverse transcriptase (Invitrogen) according to instruction of the manufacturer ...
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bioRxiv - Genomics 2019Quote: ... Probes were mixed with Human Cot-1 DNA (Invitrogen, 15279011) and salmon sperm (Invitrogen ...