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Citations for Thermo Fisher :
7401 - 7450 of 10000+ citations for 6H 1 3 Dioxolo 4 5 g 1 benzopyran 6 one 7 8 dihydro since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2022Quote: ... CR9114 was expressed in 293T cells and purified 4 days post-infection using NAB protein A/G spin kits (no. 89950; Thermo Fisher).
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bioRxiv - Biochemistry 2022Quote: ... a total of 120 μg of protein from lysates was migrated on a pre-cast 4-12% Novex NuPAGE SDS Mini Gel (Invitrogen/Thermo Fisher) using the Bis/Tris buffer system (product number NP0326BOX ...
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bioRxiv - Neuroscience 2020Quote: ... at 4 ng/μL and then pulled-down for 2 h using protein G-coupled dynabeads (50 μL, Thermo Fisher Scientific). After washing 3 times with 0.3 M NaCl solution ...
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bioRxiv - Cancer Biology 2021Quote: ... Lysates (10 µg of protein/well) were run on 20-well 4-12% NuPage BisTris midi gels in 1X MES buffer (ThermoFisher Scientific) and transferred to polyvinylidene difluoride (PVDF ...
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bioRxiv - Biophysics 2021Quote: ... Cells were harvested by centrifugation at 5,000 x g for 15 min at 4 °C in a Fiberlite F9 rotor (ThermoFisher, cat. # 13456093). Cells were lysed by sonication in lysis buffer (20 mM Tris-HCl pH 8.0 ...
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bioRxiv - Cell Biology 2022Quote: ... for 1 hr at 4°C, then proceeded for immunoprecipitation with specific antibodies (Rab5A, Rab 11 and Rab7) and Protein A/G bead (Thermo Fisher) for overnight at 4°C ...
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bioRxiv - Systems Biology 2022Quote: ... equal amounts of protein lysates were incubated with pull-down antibody overnight at 4°C followed by 2 h treatment with Dynabeads Protein G magnetic beads (10004D, ThermoFisher, MA) for pull down ...
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bioRxiv - Neuroscience 2019Quote: ... A total of 30 µg of protein per sample was separated on 4-12% Bis-Tris gradient gels in MOPS SDS running buffer (Thermo Fisher) at 100 V for 3 h followed by transfer to nitrocellulose membrane at 120 V for 1.5 h at room temperature ...
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bioRxiv - Microbiology 2020Quote: ... Lysates were centrifuged (14.000x g, 10 min at 4°C) and soluble protein concentrations were determined by a Bradford assay (Thermo Fisher Scientific). For digestion ...
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bioRxiv - Microbiology 2020Quote: HAP- or ΔXKR8-produced purified rVSV/G or rVSV/EBOV-GP (1.5×109 genomes) were conjugated to 4-μm aldehyde/sulfate latex beads (Thermo Fisher Scientific) overnight at 4°C with gentle shaking ...
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bioRxiv - Developmental Biology 2020Quote: ... Sheared chromatin was cleared and incubated overnight at 4 degrees with primary antibodies that were pre-incubated with protein G magnetic DynaBeads (Thermo Fisher). Primary antibodies used included anti-H3K27Ac (9ug/sample ...
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bioRxiv - Immunology 2021Quote: ... The filtrate was collected in sterile tubes and the cells were pelleted by centrifugation at 4°C for 10min at 250×g in a centrifuge (Thermo Scientific). The pellet containing red blood cells (RBCs ...
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bioRxiv - Immunology 2020Quote: ... Lysates were cleared by centrifugation at 20,000 g for 15 min at 4°C then protein concentrations determined using Coomassie Protein Assay Kit (Thermo Scientific, UK). For each sample ...
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bioRxiv - Biochemistry 2019Quote: ... Cells were harvested by centrifugation at 5,000 x g for 10 min at 4°C in a Fiberlite F9 rotor (ThermoFisher, cat.# 13456093).
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bioRxiv - Cancer Biology 2019Quote: ... or IgG (mouse or rabbit based on the antibody used for the immunoprecipitation) for 4 hours followed by addition of 20 μl protein A/G agarose beads (ThermoFisher Scientific) for 2 hours ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... The lysate was clarified via centrifugation at 16,100 × g for 10 min at 4°C then total protein concentration was measured using a detergent-compatible Bradford assay (ThermoFisher 23246). Lysate was transferred to a 384-well plate (30 μL × 3 TR-FRET replicates ...
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bioRxiv - Cell Biology 2020Quote: ... Lysates were centrifuged at 13800 x g for 15 min at 4°C and supernatants quantified by Bradford assay (Thermo Scientific) and 60 μg and 400 μg of proteins used for immunoblotting and coimmunoprecipitation assay ...
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bioRxiv - Immunology 2020Quote: ... Equal amounts of protein (20-30 μg) were loaded on NuPAGE Bis-Tris 4%–12% gradient polyacrylamide gels in the MOPS or MES buffer system (ThermoFisher Scientific) and subsequently electrotransferred from mini gels to a solid nitrocellulose membrane using the iBlot 2 system (ThermoFisher Scientific) ...
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bioRxiv - Cell Biology 2021Quote: ... Soluble proteins were retrieved after centrifugation at 15000 g at 4°C for 10 minutes and quantified using the Coomassie protein assay (Thermo Scientific). 25 µg of proteins were separated on a NuPAGE 4% -12% gel (Invitrogen ...
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bioRxiv - Cancer Biology 2020Quote: ... centrifuged at 4 °C and 12000 x g for ten minutes (Heraeus Fresco 17 Centrifuge Refrigerated, Thermo Scientific, Waltham MA, USA) to remove crude debris.
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bioRxiv - Cancer Biology 2022Quote: ... sonicated chromatin was incubated overnight at 4°C with antibodies that had first been coupled to Protein G beads (Thermo Fisher). After incubation ...
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bioRxiv - Neuroscience 2022Quote: Approximately 10 μg of synaptosome protein was separated on a precast gradient gel (NuPAGE 4-12% Bis-Tris Protein Gels, NP0336BOX, Thermo Fisher) and transferred to nitrocellulose membrane (AmershamTM Protran® Western Blotting Membrane ...
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bioRxiv - Molecular Biology 2023Quote: ... Lysates were cleared by centrifugation at 21,000 x g for 10 minutes at 4°C and protein concentration was determined by Bicinchoninic acid assay (Pierce, ThermoFisher Scientific). Proteins were reduced with 5 mM dithiothreitol for 30 minutes at 55°C ...
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bioRxiv - Molecular Biology 2023Quote: ... 2 mg of total protein was incubated with the DNA-PKcs monoclonal antibody (25–4) and Protein A/G beads (Thermo Fisher) overnight at 4° C with mixing ...
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bioRxiv - Physiology 2022Quote: ... and loaded at 1.08 μg (Aug. 2021) or 1.3 μg (Aug. 2022) protein per well into a 4– 12% tris-glycine gel (Thermo Fisher Scientific). Electrophoresis was performed for 30 minutes at 60 V followed by an hour at 160 V ...
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bioRxiv - Neuroscience 2022Quote: ... Protein lysates were pre-cleared 1h at 4°C on a rotating wheel with 50μl of previously washed Dynabeads Protein G (Invitrogen, Thermo Fisher Scientific), to avoid aspecific bindings ...
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bioRxiv - Neuroscience 2022Quote: ... followed by overnight incubation at 4°C with either 10 μl of pre-equilibrated protein A or G Ultralink beads (Thermo Scientific). The resin was washed several times with immunoprecipitation buffer spinning at 700 g for 30 sec ...
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Identification of echinacoside as a tobramycin potentiator against Pseudomonas aeruginosa aggregatesbioRxiv - Microbiology 2024Quote: ... Samples were then pelleted at 5000×g at 4 for 15 mins and re-suspended in 200 µL of freshly prepared 25 mg/mL lysozyme (Thermo Fisher) dissolved in 1×Tris-EDTA buffer (Fisher) ...
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Parkinson’s VPS35[D620N] mutation induces LRRK2 mediated lysosomal association of RILPL1 and TMEM55BbioRxiv - Neuroscience 2023Quote: ... 10-20 µg of lysate or 25% of the immunoprecipitated samples were loaded onto NuPAGE 4–12% Bis–Tris Midi Gels (Thermo Fisher Scientific ...
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bioRxiv - Biochemistry 2023Quote: ... Lysates were centrifuged at 20,000 x g for 10 min at 4 °C and protein concentrations were determined using Pierce BCA Protein Assay Kit (ThermoFisher Scientific) according to manufacturer’s instructions ...
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bioRxiv - Immunology 2023Quote: ... The soluble fractions of cell lysates were isolated by centrifugation at 20,000 g at 4 °C for 10 min and quantified by a BCA kit (Thermo Fisher Scientific). Protein samples were denatured with the addition of 6 × SDS sampling buffer and incubated at 95 °C for 5 min ...
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bioRxiv - Microbiology 2023Quote: ... The supernatant was centrifuged at 15,000 ×g at 4 °C for 15 min with Sorvall LYNX4000 (Thermo Scientific, Waltham, MA, USA). To remove the remaining bacterial cells ...
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bioRxiv - Genomics 2023Quote: ... or 4 μl of rabbit polyclonal anti-GAF [101] antibodies per sample were immobilized on Protein G Dynabeads (Thermo Fisher Scientific), incubated with lysates for 90 min at 25°C ...
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bioRxiv - Cell Biology 2023Quote: ... The lysates were centrifuged at 14,000 x g at 4 °C and RNA concentration was measured using Qubit BR RNA Assay Kits (ThermoFisher Scientific, Q10210). For IP ...
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bioRxiv - Genomics 2023Quote: ... Immunoprecipitations were performed overnight at 4°C on 40μg of chromatin with antibodies pre-bound to Dynabeads protein G (Thermo Fisher Scientific). The antibodies used are listed in Table S10 ...
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bioRxiv - Genetics 2023Quote: ... Equal amounts of protein (30-50 μg) were loaded on NuPAGE Bis-Tris 4%–12% gradient polyacrylamide gels in the MOPS or MES buffer system (ThermoFisher Scientific) and subsequently electrotransferred from mini gels to a solid nitrocellulose membrane using the iBlot 2 system (ThermoFisher Scientific) ...
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bioRxiv - Microbiology 2023Quote: ... Lysates were clarified by centrifugation (15,000 x g, 20 min, 4 °C) and their concentrations quantified via the BCA assay (Thermo Fisher Scientific). Lysates were run on NuPAGE 4-12% Bis-Tris protein gels (Thermo Fisher Scientific ...
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bioRxiv - Cell Biology 2023Quote: ... Debris was removed by centrifugation (4°C, 30 min, 20’000 g) and total protein concentrations measured using the Bradford protein assay (Thermo Fisher Scientific). Typically ...
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bioRxiv - Cancer Biology 2023Quote: ... Cell lysates were incubated overnight at 4 °C with mouse anti-ee antibodies pre-bound to Dynabeads protein G (Thermo Fisher Scientific( ...
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bioRxiv - Molecular Biology 2023Quote: ... 10µg of protein was loaded per well and run on a 4- 12% bis-tris precast SDS page gel (Invitrogen, NP0323BOX). Semi-dry transfer was performed using the TransBlot Turbo transfer system (BioRad ...
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bioRxiv - Microbiology 2023Quote: ... Lysates from the secretomes and intracellular fractions were centrifuged (10 min, 14,000 g, 4 °C) and soluble protein concentrations were determined (Bradford assay, Thermo Fisher Scientific). For digestion ...
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bioRxiv - Biochemistry 2024Quote: Proteins (between 5 and 10µg) from tissue preparations were solubilized in Laemmli buffer before loading on top of a 4–12% NuPAGE gel (Life Technologies), stained with R-250 Coomassie blue (Bio-Rad ...
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bioRxiv - Molecular Biology 2024Quote: ... The supernatant was precleared for on a rotating wheel at 16 rpm 30 min at 4°C with 10 µl of Protein G dynabeads (Thermo Fisher) that were previously washed with 100 µl of RIPA buffer (10□mM Tris–HCl pH 8.0 ...
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bioRxiv - Biophysics 2024Quote: ... Cells were harvested via centrifugation at 5000 x g for 15 min at 4 °C using a Sorvall BIOS 16 centrifuge (Thermo Scientific). Pellets were flash frozen in liquid nitrogen and stored at -80 °C.
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bioRxiv - Biophysics 2024Quote: ... Cells were harvested via centrifugation at 5000 x g for 15 min at 4 °C using a Sorvall BIOS 16 centrifuge (Thermo Scientific). Pellets were flash frozen in liquid nitrogen and stored at -80 °C.
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bioRxiv - Biophysics 2024Quote: ... Cells were harvested via centrifugation at 5000 x g for 15 min at 4 °C using a Sorvall BIOS 16 centrifuge (Thermo Scientific). Pellets were flash frozen in liquid nitrogen and stored at -80 °C.
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bioRxiv - Molecular Biology 2024Quote: ... The lysate was cleared by centrifugation at 21 130 x g at 4 °C for 30 min and protein content quantified using the Pierce BCA protein assay kit (ThermoFisher Scientfic). Twenty µg of the lysate ...
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bioRxiv - Microbiology 2024Quote: ... Supernatants were then pre-cleared twice for 30 min with Protein G sepharose 4 fast flow (Fisher Scientific 45-000-116) at 4°C before either antibody (mouse anti-FLAG ...
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bioRxiv - Biophysics 2021Quote: MDCK-II wild type canine kidney epithelial cells were a gift from the Nelson Laboratory at Stanford University and were cultured in customized media consisting of low-glucose (1 g/L) DMEM with phenol red (Gibco, USA; powder stock), 1 g/L sodium bicarbonate (lower than standard DMEM) ...
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bioRxiv - Molecular Biology 2021Quote: ... and their derivatives were cultured in DMEM containing 1 g/l glucose supplemented with 10% heat-inactivated FCS (Fetal Calf Serum, Gibco, Cat#: 10270-106), 2 mM L-glutamine (Thermo Scientific ...