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Citations for Thermo Fisher :
7351 - 7400 of 10000+ citations for 6H 1 3 Dioxolo 4 5 g 1 benzopyran 6 one 7 8 dihydro since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2024Quote: ... overnight incubation at 4°C was performed 1:500 dilution of goat anti-rabbit secondary antibody AlexaFluor 488 (Invitrogen) and goat anti-mouse secondary antibody AlexaFluor 488 (Invitrogen) ...
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bioRxiv - Neuroscience 2024Quote: ... the slices were incubated overnight at 4 °C in a primary antibody solution (polyclonal rabbit anti-zo-1, Invitrogen Cat# 40-2200 ...
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bioRxiv - Neuroscience 2024Quote: ... incubated with the secondary antibody solution at 4 °C overnight (anti-mouse Alexa Fluor 488 (1:400; Invitrogen; A11001), anti-mouse Cy3 (1:1000 ...
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bioRxiv - Cancer Biology 2024Quote: ... Gels were then run at 4°C in 0.5X TBE before staining in 1/10000 SYBR Gold (ThermoFisher Scientific) for 1 hour at room temperature on a rocker and visualization on the Typhoon 9400 Variable Mode Imager using the A488 channel.
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bioRxiv - Biophysics 2023Quote: ... sonicated plasma membranes were fixed with 4% PFA before incubation with primary (GFP Thermo Fisher A11122 Rabbit 1/20) and secondary antibodies coupled to gold beads (rabbit-gold 815.011 AURION goat 1/25) ...
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bioRxiv - Bioengineering 2023Quote: ... monolayers reaching desired time-points were rinsed in 1× PBS followed by fixing in 4 % formaldehyde (28906, Thermofisher Scientific) for 15 minutes ...
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bioRxiv - Neuroscience 2023Quote: ... and 1% n-Dodecyl-β-D-maltoside were loaded onto Novex 4-16% Bis/Tris gradient gels (Life Technologies). When assaying stoichiometry of AMPAR-auxiliary subunit complexes ...
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bioRxiv - Cell Biology 2023Quote: ... was maintained in a medium composed of 4:1 of Dulbecco’s minimal essential medium (DMEM, Gibco, Invitrogen, NY, USA) and Medium 199 ...
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bioRxiv - Neuroscience 2023Quote: iPSC-derived neurons were washed with Krebs HEPES Buffer and incubated with 1 μm Fluo 4-AM (Thermofisher F14201) in Krebs HEPES Buffer for 20 min at room temperature ...
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bioRxiv - Plant Biology 2023Quote: ... at 4°C overnight and the secondary goat anti-rabbit-Alexa Fluor 488 antibody (1:300, Molecular Probes, A11008) at 37°C for 90 min ...
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bioRxiv - Cell Biology 2023Quote: ... was maintained in a medium composed of 4:1 of Dulbecco’s minimal essential medium (DMEM, Gibco, Invitrogen, NY, USA) and Medium 199 ...
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bioRxiv - Molecular Biology 2023Quote: ... Brain slices were then incubated overnight at 4 °C with the rabbit anti-GFP primary antibody (1:1000, Invitrogen) diluted in blocking solution ...
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bioRxiv - Molecular Biology 2023Quote: ... and cells were then incubated overnight at 4 °C with the rabbit anti-GFP primary antibody (1:1000, Invitrogen) diluted in 3 % BSA in PBS ...
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bioRxiv - Neuroscience 2023Quote: ... fixed in 4% PFA for 10 minutes at room temperature and incubated in 1 μg/ml DAPI (Life Technologies) for 10 minutes at room temperature in DPBS+/+ ...
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bioRxiv - Cell Biology 2022Quote: ... mixed with PBS (4:1) adjusted to a concentration of 17,000 cells/µl and cultured in culture medium Advanced DMEM/F12 (Gibco), Primocin 50 µg/ml (Invivogen) ...
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bioRxiv - Cancer Biology 2023Quote: ... the medium was changed to differentiation medium containing DMEM/M199 at a 4:1 ratio (Gibco, 10566016 and 31150022), 20 mM Hepes (Biochrom ...
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bioRxiv - Cell Biology 2023Quote: ... 1 mM MnCl2 with a final assay concentration of 75 µM 6,8-difluoro-4-methylumbelliferyl phosphate (DiFMUP) (Invitrogen D6567). Generation of 6,8-difluro-4-methylumbelliferone (DiFMU ...
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bioRxiv - Immunology 2023Quote: ... sequences of HLA-A*02:01:01:01 exons 1-4 (GenBank no. HG794376.1) were synthesized (Thermo Fisher Scientific) and cloned into two previously described TÜ165-CAR-epHIV7 vectors by using NheI and RsrII restriction sites to ...
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bioRxiv - Neuroscience 2023Quote: ... and were incubated overnight at 4°C in polyclonal rabbit IgG fraction anti-Lucifer yellow (1:500, Life Technologies - Thermo Fisher Scientific ...
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bioRxiv - Neuroscience 2023Quote: ... slices were incubated overnight at 4#x00B0;C with rabbit anti-mCherry primary antibody (1:200, Invitrogen, #PA5-34974). Sections were then washed with PBS and incubated for 2 hr at room temperature with a secondary antibody (1:500 ...
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bioRxiv - Neuroscience 2023Quote: ... sections were washed in 0.1M PBS again with the same sequence and incubated with agitation in the dark for 2 hrs in 0.4% Triton-X in 0.1M PBS with secondary antibodies: goat anti-chicken IgG (Alexa Fluor 488; 1:600; Invitrogen) and for Fos detection ...
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bioRxiv - Molecular Biology 2023Quote: ... 1×105 HEK 293T cells were plated on each respective well of a 4 well chamber slide (Thermofisher Scientific) and incubated overnight at 37°C with 5% CO2 ...
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bioRxiv - Cell Biology 2023Quote: ... monolayers reaching desired time-points were rinsed in 1× PBS followed by fixing in 4 % formaldehyde (28906, Thermofisher Scientific) for 15 minutes ...
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bioRxiv - Immunology 2023Quote: ... purified cells were plated in 96-well round bottom plate in Yssel’s media (IMDM + 4% v/v Yssel’s supplement (made in house, Amsterdam UMC) + 1% v/v human AB serum (Invitrogen)) ...
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bioRxiv - Cell Biology 2023Quote: ... lysates were separated by SDS-PAGE gel electrophoresis on 1 mM NuPAGE 4 – 12% bis-tris gels (Thermo Fisher) using MOPS running buffer and transferred to nitrocellulose membranes using Tris-Glycine-methanol transfer buffer (30.3 g Tris Base ...
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bioRxiv - Cancer Biology 2023Quote: ... the membrane was stained using the respective antibody (MGMT: 1:500 dilution, 4°C overnight incubation, #MA5-13506, Invitrogen, Waltham ...
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bioRxiv - Cell Biology 2023Quote: ... Immunostaining was performed at 4 °C for Scribble and CHIKV proteins using anti-Scrib (1:500, Invitrogen PA5-54821), anti-E2 (1:500 ...
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bioRxiv - Microbiology 2024Quote: ... cells were plated at 1×106 in 4 10cm plates 24 hours prior to harvest through trypsinization (ThermoFisher Scientific) for adherent cells ...
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bioRxiv - Developmental Biology 2024Quote: Tissue samples were fixed overnight at +4°C in periodate–lysine–paraformaldehyde (PLP) buffer [1% paraformaldehyde (Thermo Scientific, 28908), 0.01M sodium periodate (Sigma ...
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bioRxiv - Cell Biology 2024Quote: ... 1:1000 primary antibody overnight at 4°C and a AlexaFluor goat anti-rabbit secondary antibody (ThermoFisher, A-11008) at 1:500 for 4 hours at RT with PBS washes in between antibody steps.
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bioRxiv - Bioengineering 2024Quote: ... PEGαMA hydrogels were made by dissolving the PEGαMA in pH 8.4 4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid (HEPES; Life Technologies) at 12.5-15.5 weight percent (wt%) ...
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bioRxiv - Neuroscience 2024Quote: ... slices were washed and stained for 1-4 hours with goat anti-chicken Alexa Fluor 488 (A-11039, Invitrogen) secondary solution ...
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bioRxiv - Bioengineering 2024Quote: ... Protein (10 μg) from each sample was combined with 1X Bolt LDS Sample Buffer (1:4, Invitrogen catalog #B0007) and 1X Bolt Reducing Agent (1:10 ...
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bioRxiv - Cell Biology 2024Quote: ... 1 µg or 4 µg of modRNA was mixed with Opti-MEM reduced serum medium plus GlutaMAX (ThermoFisher Scientific) and Lipofectamine RNAiMAX (ThermoFisher Scientific ...
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bioRxiv - Cell Biology 2024Quote: ... MDA-MB-231 cells were co-transfected with pXPR_001-sgRab21 and pUC57-Rab21 HDR plasmids in 1:4 ratio using Lipofectamine 3000 (Invitrogen). Five days post-transfection ...
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bioRxiv - Physiology 2019Quote: ... 1 μL of a FAM-labeled MT-ATP 8-probe or 1 μL of a MVIC-labeled GAPDH-probe (both probes from Applied Biosystems, Branchburg, New Jersey, USA), and 2 μL of TE buffer containing cf DNA isolated from plasma ...
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bioRxiv - Microbiology 2020Quote: Purified PFABN was suspended in water at a concentration of 1 mg/mL and transferred to 8 mm crimp top glass vials (Fisher Scientific; Cat. No.: C4008-632C). 175 μL of 2 M trifluoroacetic acid containing 15 ng of D6-myo-inositol was added and the vials were capped with Teflon-coated aluminum caps (Fisher Scientific ...
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bioRxiv - Cell Biology 2019Quote: ... the cell lysates were centrifuged at 12,000 g for 15 min at 4 °C and the supernatant was precleared with Pierce Protein A/G Magnetic Beads (88802, Thermo scientific) for 15 min on ice and then incubated with 2 μg anti-CRIPT antibody at 4 °C for 2 hours and with Pierce Protein A/G Magnetic Beads for another 2 hours ...
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bioRxiv - Microbiology 2020Quote: ... 500 µl of the subculture was centrifuged for 4 min at 2’000 x g and the bacterial pellet was resuspended in 500 µl of M199 (Gibco, 22340-020) supplemented with 10% of heat-inactivated fetal calf serum (FCS) ...
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bioRxiv - Immunology 2022Quote: ... serum Igs were purified from H10ssF-immunized mice (combined 2-4 weeks after the third immunization) by using protein A/G (Pierce Thermo Scientific). Seven mg of purified heperimmune Igs or control Igs or 100 μg (5 mg kg-1 ...
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bioRxiv - Genomics 2020Quote: ... diluted chromatin was incubated overnight at 4°C with Protein A and Protein G Dynabeads (ThermoFisher Scientific 10002D, 10004D; 25µL each) and indicated antibodies (Key Resources Table) ...
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bioRxiv - Immunology 2019Quote: ... Gastric homogenates were centrifuged at 13,000 × g at 4°C and the supernatants collected for analysis by the Qubit™ protein assay (ThermoFisher Scientific) and ELISA.
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bioRxiv - Synthetic Biology 2021Quote: ... Immunoprecipitation was performed for 16h at 4°C on a rotating platform by incubating with Dynabeads protein-G (Thermo Fisher Scientific) conjugated with antibodies ...
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bioRxiv - Developmental Biology 2020Quote: ... Immunoprecipitation was performed for 16h at 4°C on a rotating platform by incubating with Dynabeads protein-G (Thermo Fisher Scientific) conjugated with 5 μg of one of the following antibodies ...
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bioRxiv - Biophysics 2020Quote: ... The expression culture was finally centrifuged for 10 min at 4°C and 5316 × g (Thermo Scientific H-12000 BioProcessing Rotor). The cell pellet was separated from the supernatant and resuspended with 40 ml HEPES-buffer per 2 l culture on ice ...
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bioRxiv - Systems Biology 2021Quote: ... Lysates were cleared by centrifugation at 16,000 x g for 10 minutes at 4°C and protein concentration was determined using the BCA protein assay kit (Thermo Scientific). Fifty micrograms of protein was reduced with 5 mM Tris 2-carboxyethylphosphine (TCEP ...
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bioRxiv - Biophysics 2020Quote: ... Cells were harvested by centrifugation at 5,000 x g for 15 min at 4 °C in a Fiberlite F9 rotor (ThermoFisher, cat. # 13456093). Cells were lysed by sonication in lysis buffer (20 mM Tris-HCl pH 8.0 ...
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bioRxiv - Cell Biology 2021Quote: ... Lysates were clarified by centrifugation (20000 g, 10 minutes, 4 °C) and protein concentrations were determined using the BCA protein assay kit (ThermoFisher Scientific). The same amount of protein (1.2 μg ...
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bioRxiv - Neuroscience 2022Quote: ... Samples were centrifuged (14000 g, 30 min, 4 °C) and protein concentration in the supernatant was determined with the BCA method (ThermoFisher, Germany). After boiling with 4x Laemmli buffer (1 M Tris pH 6.8 ...
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bioRxiv - Neuroscience 2022Quote: Cell culture supernatants from cerebral cortex neurons were centrifuged at 10,000 g and 4°C and subjected either to protein precipitation or to immunoprecipitation using Reelin antibody G10 and Dynabeads Protein G (ThermoFisher Scientific).