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Citations for Thermo Fisher :
7351 - 7400 of 10000+ citations for Glutathione Reductase Fluorescent Kit 1 Plate since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2021Quote: ... The cells were washed with PBS and resuspended in 200 μL FACS buffer with 1 μL of LIVE/DEAD Fixable Aqua Dead Cell Stain Kit (Invitrogen). HeLa and HeLa-ACE2 cells alone and with SARS-CoV-2 Spike only were used as background and positive controls ...
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bioRxiv - Immunology 2020Quote: ... cDNA was synthesized using 1 μg of RNA using the Superscript III First Strand Synthesis Kit (Life Technologies/Thermo Fisher). RNA quality was determined using the 2100 Bioanalyzer (Agilent) ...
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bioRxiv - Immunology 2020Quote: ... cDNA was synthesized using 1 μg of RNA using the Superscript III First Strand Synthesis Kit (Life Technologies/Thermo Fisher). RNA quality was determined using the 2100 Bioanalyzer (Agilent) ...
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bioRxiv - Genomics 2021Quote: ... The lce.2 cDNA was amplified from the reverse transcribed template via PCR (see primer sequences in Supplementary Text 1) and inserted into a TOPO TA cloning kit vector (Invitrogen) according to manufacturer’s instructions ...
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bioRxiv - Genetics 2020Quote: ... Real-time quantitative PCR were performed on 1ml of diluted RT reactions (1/5) using SYBR Green kit (Life Technologies) on a One Step Plus thermocycler (Thermo Fisher) ...
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bioRxiv - Microbiology 2020Quote: ... The concentration of double strand cDNA was evaluated (Table 1) using the Qubit® dsDNA HS Assay Kit (Life Technologies). For six DNA samples ...
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bioRxiv - Neuroscience 2022Quote: Total RNA was isolated from the primary fibroblast cultures derived from patient PFS and 4 healthy unrelated gender-matched controls (3 homozygous for wild-type C allele at rs324420 and 1 heterozygous C/A) using the PureLink RNA Micro Kit (Invitrogen) and run by Eurofins on the human Clariom D transcriptomic array (Thermo Fisher ...
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bioRxiv - Bioengineering 2022Quote: ... cDNA was synthesized from ∼1 µg of total RNA using the High-Capacity RNA-to-cDNA Kit (ThermoFisher Scientific, 4387406) per manufacturer’s protocols ...
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bioRxiv - Bioengineering 2022Quote: ... using a ratio of 1 µg trypsin to 50 µg sample protein as quantified using a Pierce BCA Protein Assay Kit (ThermoFisher). Samples were digested overnight at room temperature using a rotor for mixing and then placed in a speed vacuum to reduce sample volume ...
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bioRxiv - Cancer Biology 2022Quote: ... Total RNA (1 µg) was converted to cDNA using a High-Capacity cDNA Reverse Transcription Kit (ThermoFisher Scientific, Cat. #4368813) according to the manufacturer’s protocol ...
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bioRxiv - Molecular Biology 2022Quote: ... for RT-qPCR gene expression analysis was synthesized from 1 µg of total RNA using the SuperScript VILO cDNA Synthesis Kit (Invitrogen) according to the manufacturer’s guidelines ...
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bioRxiv - Bioengineering 2022Quote: ... and 1 µg of total RNA was reverse-transcribed using the High-Capacity RNA-to-cDNA kit (Applied Biosystems, 4387406) according to the manufacturer’s instructions ...
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bioRxiv - Biophysics 2022Quote: ... cDNA synthesis was carried out using 1 μg of RNA and the High Capacity cDNA Reverse Transcription Kit (Applied Biosystems) according to manufacturer’s instructions ...
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bioRxiv - Biochemistry 2022Quote: ... At least 1 × 106 colonies were scrape-harvested with LB broth and plasmids were extracted using a PureLink Plasmid Midiprep kit (Invitrogen).
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bioRxiv - Cancer Biology 2022Quote: ... 1 µg total RNA was used in cDNA synthesis using RevertAid First Strand cDNA Synthesis Kit (Thermo Fisher Scientific#K1622) following manufacturer’s instruction ...
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bioRxiv - Cancer Biology 2022Quote: ... Synthesis of cDNA was performed using 0.2-1 µg total RNA and the High Capacity cDNA Reverse Transcription kit (Applied Biosystems). Quantitative real-time PCR reactions were performed using SYBR® Green on the Step One plus Real-time PCR system (Applied Biosystems) ...
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bioRxiv - Microbiology 2022Quote: ... Cell re-suspensions were stained using the LIVE/DEAD Fixable Aqua Dead Cell Stain kit (1:1,000; Thermo Fisher Scientific) at room temperature for 30 mins ...
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Allelic diversity uncovers protein domains contributing to the emergence of antimicrobial resistancebioRxiv - Microbiology 2022Quote: ... Samples were diluted 1:5 in sample loading buffer and quantified using the Pierce BCA Protein Assay Kit (Thermo Scientific). Lysates were normalized to 100μg/mL in 1X Bolt™ LDS Sample Buffer (Invitrogen) ...
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bioRxiv - Microbiology 2022Quote: ... or with 200 μL of Exosome Resuspension Buffer (1-2.5% Triton X-100) from the Total Exosome RNA and Protein Isolation Kit (Invitrogen, Thermo-Fisher Scientific) to lyse the EVs for proteomic analysis.
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bioRxiv - Plant Biology 2022Quote: ... 1 μg of total RNA was used for reverse transcription reactions using RevertAid RT Reverse Transcription Kit (Thermo Fisher Scientific). qPCR was performed using SYBR green (Thermo Scientific Maxima SYBR Green qPCR Master Mix (2x)) ...
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bioRxiv - Plant Biology 2022Quote: ... PCR products were run on 1% agarose gel and fragments of the correct size were extracted and purified using the GeneJET Gel Extraction Kit (ThermoFisher) following manufacturer instructions ...
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bioRxiv - Neuroscience 2023Quote: ... The synthesis of single strand cDNA and quantification of each mRNA was performed using the TaqMan RNA-to Ct 1-step Kit (#4392938, ThermoFisher) a ViiA Real-Time PCR System (Applied Biosystems) ...
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bioRxiv - Physiology 2023Quote: ... 1 µg RNA was reverse transcribed into cDNA using High-Capacity cDNA Reverse Transcription Kit (Thermo Fisher Scientific, Massachusetts, USA). The gene expression level relative to β-actin was determined by qPCR with gene-specific primers (Supplementary Table S1 ...
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bioRxiv - Plant Biology 2023Quote: ... First-strand cDNA was synthesized from 1 μg of total RNA using a Thermo Scientific RevertAid RT Kit (Thermo Scientific). Quantitative RT-PCR was performed using a CFX Connect Real-Time PCR Detection System (Bio-Rad ...
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bioRxiv - Microbiology 2022Quote: ... Vector propagation was performed in 1.5 mL LB (low salt + Zeocin 75 μg mL−1) and GeneJET PCR Purification Kit (ThermoFisher, Germany). P ...
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bioRxiv - Cancer Biology 2024Quote: ... cDNA was synthesized from 1 μg of total RNA using Maxima First Strand cDNA Synthesis Kit (K1672; Thermo Fisher Scientific). RT-qPCR was performed using Applied Biosystems™ PowerUP™ SYBR™ Green Master Mix (A25778 ...
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bioRxiv - Neuroscience 2022Quote: ... 1 ng-5 μg of RNA per reaction was used in SuperScript™ First-Strand synthesis kits (Invitrogen, ID: 11904018). Nucleic acid purity was assessed using a NanoPhotometer® (Implen).
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bioRxiv - Neuroscience 2022Quote: ... and 1 μg RNA was reverse-transcribed with oligo-dT18 using the First Strand cDNA Synthesis Kit (Thermo Fisher Scientific). The coding sequence of full-length hnRNP R was then PCR-amplified from the cDNA using the KAPA HiFi HotStart ReadyMix (Roche ...
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bioRxiv - Molecular Biology 2022Quote: ... The unspiked and spiked samples were then subjected to amplification using the Verso 1-step RT-PCR kit (Thermo Scientific); the reaction was set up in 10 μl final volumes with 600 nM of labelled triplex primer cocktail and 1-3 μl of RNA template ...
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bioRxiv - Developmental Biology 2022Quote: ... 48 hpf embryos were incubated at 4°C for 1 h in E3 supplemented with 500 μm of EdU (ClickiT-Alexa 488 fluorophore kit, Invitrogen) in 10% DMSO ...
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bioRxiv - Synthetic Biology 2024Quote: ... cell pellets were flash-frozen and stored in -80℃ before processing for cell lysis and analysis by Cells-to-CT 1-Step TaqMan Kit with Taqman probes (ThermoFisher). For Western blots ...
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bioRxiv - Microbiology 2024Quote: ... Genomic DNA concentration was quantified using a NanoDrop One spectrophotometer (Thermo) and 1 ng of DNA was amplified by PCR using the AmpFℓSTR Identifiler Plus PCR Amplification Kit (Applied Biosystems) following the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2024Quote: ... lines (Table 1) were generated from blood collected from three control subjects and three ARXPAE patients using an episomal reprogramming kit (Invitrogen) or CytoTune-iPS Sendai Reprogramming kit (Thermofisher ...
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bioRxiv - Physiology 2024Quote: ... The samples were filtered through a silicon membrane (1 μm retention limit, Microplastic Sample Preparation Kit, Thermo Fisher Nepean, ON) using glass vacuum filtration funnel ...
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bioRxiv - Neuroscience 2024Quote: ... 1 µg total RNA of each sample was reverse transcribed (High capacity cDNA reverse transcription kit, Thermo Fisher Scientific, USA), and cDNA concentrations were determined (Qubit ssDNA assay kit ...
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bioRxiv - Immunology 2024Quote: ... Full differentiation into mature MCs was confirmed by expression of lineage-specific c-KIT (rat anti-CD117 FITC-conjugated, 1:200, Invitrogen) and FcεRI (armenian hamster anti-FcεRIα Alexa Fluor 647-conjugated ...
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bioRxiv - Developmental Biology 2024Quote: ... cDNA was synthesized from 1 μg of total RNA using a High-Capacity cDNA Reverse Transcription Kit (4368813; Thermo Fisher) and diluted 10 times with Milli-Q water ...
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bioRxiv - Developmental Biology 2024Quote: ... RT-qPCR analysis was performed using Power SYBR Green RNA to CT 1 step RT-PCR kit (Applied Biosystems, #4389986) on a QuantStudio 3 Real-Time PCR System (Applied Biosystems ...
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bioRxiv - Molecular Biology 2023Quote: ... The purified RNA (1 μg) was converted to cDNA by random hexamers using Verso c-DNA Kit (Thermo Fisher Scientific) following manufacturers’ protocol ...
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bioRxiv - Immunology 2023Quote: ... RNA (1 μg) was reverse transcribed to make complementary DNA using SuperScript™ VILO™ cDNA Synthesis Kit (Invitrogen, 11754) using random primers ...
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bioRxiv - Immunology 2024Quote: ... The CCL2 levels in tumor lysate was measured by MCP-1 Mouse Uncoated ELISA Kit (Thermo Scientific, #88-7391-22) with comparable sized tumors (∼3 mm diameter) ...
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bioRxiv - Microbiology 2022Quote: ... Reverse transcription was performed with 1 μg of total RNA using Maxima H Minus First Strand cDNA Synthesis Kit (Cat.# K1652, ThermoFisher). Quantitative PCR was done using SsoAdvanced Universal SYBR Green Supermix (1725271 ...
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bioRxiv - Microbiology 2023Quote: ... any contaminating DNA was removed by treating 1-5 μg of RNA with the TURBO DNase enzyme kit and purified according to the manufacturer’s instructions (ThermoFisher Scientific). For mouse bladder RNA samples ...
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bioRxiv - Molecular Biology 2023Quote: ... 2.5 µg of RNA was treated with 1 unit of Turbo DNase using the TURBO DNA-free Kit (AM1907, Invitrogen) and incubated for 30 min at 37°C ...
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bioRxiv - Cancer Biology 2023Quote: ... and 1 μg RNA was transcribed using the Maxima First Strand cDNA Synthesis Kit for RT-qPCR (K1641, Thermo Scientific). Quantitative real-time PCR (qRT-PCR ...
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bioRxiv - Microbiology 2023Quote: ... 5μL of the viral RNA was analyzed using a TaqMan™ RNA-to-CT™ 1-step kit (Thermo Fisher). Primers specific to the matrix gene (M ...
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bioRxiv - Cancer Biology 2023Quote: Cells were incubated with 10 μM EdU for 1 h using a Click-iT EdU Alexa Fluor 647 Flow Cytometry Assay Kit (cat. no. C10419; Invitrogen) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2023Quote: ... 1 μg of total RNA was reverse transcribed using High-Capacity cDNA reverse transcription kit (Thermo Fisher scientific, Waltham, MA) according to manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2023Quote: ... Horseradish-peroxidase conjugated secondary antibodies diluted 1:5000 in 5% non-fat milk were used and visualized with SuperSignal West Pico or Femto substrate kits (ThermoFisher). The following commercial primary antibodies supplemented with 0.02% sodium azide were used for immunoblot analysis ...
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bioRxiv - Physiology 2023Quote: ... cDNA was generated from 1 μg of total RNA using a High-Capacity cDNA Reverse Transcription Kit (Thermo Fisher Scientific). qPCR was performed using a ViiA7 system (Applied Biosystems ...