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Citations for Thermo Fisher :
7251 - 7300 of 10000+ citations for Glutathione Reductase Fluorescent Kit 1 Plate since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Bioengineering 2024Quote: ... was prepared in a black 96-well plate (ThermoFisher Scientific, USA, Cat. No. 165305) ranging from 100 to 107 CFU/well in a total volume of 100 μL ...
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bioRxiv - Bioengineering 2024Quote: ... HEK293T cells were transfected in 24-well plates using Lipofectamine 2000 (Thermo Fisher Scientific) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2024Quote: Samples were incubated in plates coated with anti-myeloperoxidase (MPO) (Thermo Fisher, #PA5-16672), washed ...
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bioRxiv - Immunology 2024Quote: ... Supernatant was removed and plates were washed with PBS containing 0.04% Tween20 (ThermoFisher Scientific) followed by dH2O three times ...
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bioRxiv - Cell Biology 2024Quote: ... IMR90 PD 34 to PD 40 were seeded on 6 well plates (ThermoFisher, 140675) as the beginning points for the assays ...
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bioRxiv - Molecular Biology 2024Quote: ... The cells were then transferred in 135μl aliquots to white 96 well plates (Nunc) containing the indicated concentrations of stressor or solvent ...
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bioRxiv - Immunology 2024Quote: ... Plates were then washed and 15 µl of CDP-substrate (Thermo Fisher Scientific #T2146) was added ...
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bioRxiv - Cancer Biology 2024Quote: ... Quantitative PCR was performed in a MicroAmp® 96-well reaction plate (Life Technologies) on a StepOne Plus Real Time PCR instrument (Thermo Fisher Scientific) ...
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bioRxiv - Cell Biology 2024Quote: ... C2C12 cells on 6-well plates were transiently transfected with RNAiMAX (Invitrogen, 13778-150) according to the manufacturer’s directions in two stages to prevent over-confluency at the time of cellular treatment and lysis ...
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bioRxiv - Developmental Biology 2024Quote: ... Cells were transfected with Lipofectamine 3000 (Invitrogen, L3000-008, Nunc 24-well plates #142475) transfection reagent ...
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bioRxiv - Immunology 2024Quote: ... Cultures were performed in V-shaped 96-well plates in RPMI 1640 (Life Technologies) supplemented with 10% FBS ...
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bioRxiv - Bioengineering 2021Quote: ... The RNA samples (1 μg each) reverse transcribed with the maxima H minus First Strand cDNA Synthesis kit (Thermo Fisher) with oligo-dT primers in a final volume of 20 μL ...
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bioRxiv - Cancer Biology 2021Quote: ... Purified total RNA (1 μg) was reverse transcribed using the superscript iii first strand synthesis supermix for qRT PCR kit (Invitrogen), according to the manufacturer’s instructions ...
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bioRxiv - Systems Biology 2020Quote: ... the total RNA was extracted from 1 μL of the reaction mixture using PureLink RNA Mini Kit (Thermo Fisher Scientific) and stored at −80°C ...
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bioRxiv - Molecular Biology 2020Quote: ... Synthesis of cDNA was performed using 0.5-1 µg total RNA and the High Capacity cDNA Reverse Transcription kit (Applied Biosystems). Quantitative real-time PCR reactions were performed using SYBR® Green on the Step One plus Real-time PCR system (Applied Biosystems) ...
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bioRxiv - Genetics 2021Quote: ... RNA (0.5 – 1 µg) was reverse transcribed to cDNA using Maxima H minus First Strand cDNA synthesis kit (ThermoFisher Scientific) using oligo dT18 (250 ng) ...
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bioRxiv - Developmental Biology 2021Quote: ... 1 ng of RNA template was subjected to cDNA synthesis using SuperScript™ VILO™ cDNA Synthesis Kit (Thermo Fisher). qPCR analysis was performed using the primers in Table S1 on CFX384 Touch Real-Time PCR Detection System (BioRad) ...
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bioRxiv - Evolutionary Biology 2021Quote: ... an antisense digoxigenin-labeled riboprobe against Nematostella vectensis polycystin 1 was synthesized by using 3’ RACE product as a template (MEGAscript transcription kit; Ambion), and was used at a final concentration of 1 ng/μl ...
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bioRxiv - Developmental Biology 2020Quote: ... 1 µg total RNA was used to synthesize the first-strand cDNA with M-MLV reverse transcription kit (Invitrogen, USA). RT-qPCR was performed on a LightCycler480 machine (Roche Diagnostics ...
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Bidirectional neuronal migration coordinates retinal morphogenesis by preventing spatial competitionbioRxiv - Developmental Biology 2021Quote: ... zebrafish embryos were incubated at 4°C for 1 h in E3 supplemented with 500 μm of EdU (ClickiT-Alexa 488 fluorophore kit, Invitrogen) while human retinal organoids were incubated at 37°C for 1h in organoid medium supplemented with 10 μm of EdU ...
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bioRxiv - Molecular Biology 2020Quote: ... equal amounts of RNA (0.5-1 μg) were reverse transcribed using the High-Capacity cDNA Reverse Transcription Kit (Applied Biosystems) with a pool of random primers or a single ...
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bioRxiv - Molecular Biology 2020Quote: ... Detection of SARS-CoV-2 N1 gene sequences and host RNase P mRNA from de-identified SARS-CoV-2 positive samples was carried out with the one-step TaqMan RNA-to-Ct 1-Step Kit (ThermoFisher), using 2 μL of undiluted PEARL extract ...
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bioRxiv - Molecular Biology 2021Quote: ... 1 μg purified DNaseI-treated RNA was used for reverse transcription (RT) (High Capacity RNA-to-cDNA kit, Applied Biosystems). Quantitative RT–PCR (qRT–PCR ...
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bioRxiv - Genomics 2020Quote: ... 1 μl of RNA solution was used to make cDNA using the High Capacity cDNA Reverse Transcription Kit (Applied Biosystems) according to manufacturer instructions ...
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bioRxiv - Molecular Biology 2019Quote: ... Templates were generated by PCR (see Supplementary Table 1 for primer sequences) and in vitro transcription was carried out using the MEGAscript T7 transcription kit (Invitrogen). RNA was isolated by phenol/chloroform extraction and ethanol precipitation.
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bioRxiv - Molecular Biology 2019Quote: ... Annealed duplexes were diluted to 1 μM in 0.1M NaCl and transcription was then carried out with the T7 MEGAshortscript kit (Thermofisher Scientific). To label standards with 5EU ...
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bioRxiv - Microbiology 2019Quote: ... RNA was purified from DNA as described previously46 and approximately 1 μg from spring samples were rRNA subtracted using the MICROBExpress Bacterial mRNA Enrichment Kit (Ambion). All RNAs were reverse transcribed following the manufacturer’s instructions for cDNA synthesis and metatranscriptome libraries prepared with an Ovation RNA-Seq System (NuGEN ...
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bioRxiv - Molecular Biology 2019Quote: ... 1 μg of RNA treated with DNase I was reverse-transcribed using the High Capacity Reverse Transcription Kit (Applied Biosystems). cDNA was quantified using SYBR Green PCR master mix (Bioline ...
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bioRxiv - Cancer Biology 2020Quote: ... 1 µg of purified RNA was used for cDNA synthesis using the High-Capacity cDNA Reverse Transcription Kit (ThermoFisher Scientific) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2019Quote: ... burnetii total RNA extracted from LCVs (3dpi) infecting Vero (carA and metK) or THP-1 (cvpD) cells using a 5’ RACE System kit (Invitrogen) according to manufacturer protocols and with gene-specific primers (see S9 Fig) ...
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bioRxiv - Immunology 2019Quote: Annealed oligonucleotides containing the sequence of the HCV 5’ppp poly-U/UC region (Table 1) (20) were in vitro transcribed using the Megashortscript T7 transcription kit (Ambion) followed by ethanol precipitation ...
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bioRxiv - Cancer Biology 2019Quote: ... The concentration of total RNA was estimated by Qubit and 1 μg of RNA was reverse transcribed into cDNA using Superscript III First strand cDNA synthesis kit (Invitrogen). 2 μl of out of 20 μl obtained cDNA was used for PCR using gene specific primers (Table 2).
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bioRxiv - Immunology 2021Quote: ... Amplification was carried out with TaqMan® RNA-to-CT™ 1-Step Kit (Applied Biosystems, Foster City, CA, USA) or Power SYBR® Green RNA-to-CT™ 1-Step Kit (ThermoFisher Scientific ...
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bioRxiv - Neuroscience 2020Quote: ... Undigested bands were gel extracted and subcloned into pJet2-1 using the Clone JET PCR Cloning kit (Thermo Fisher Scientific) and sequence-verified.
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bioRxiv - Cell Biology 2020Quote: ... 1 μg of RNA was reverse transcribed with the High-Capacity cDNA Reverse Transcription Kit (Thermo Scientific, Waltham, MA, USA). The amplified cDNA was diluted to 10 ng/μl ...
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bioRxiv - Biochemistry 2020Quote: ... first-strand cDNA synthesis was performed using 1 μg total RNA with “Superscript III First-strand Synthesis System for RT-PCR Kit” (Invitrogen) and poly-T primer ...
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bioRxiv - Bioengineering 2021Quote: ... the cells were incubated with 2 μM EthD-1 and 4 μM Calcein AM solutions of cell viability/cytotoxicity kit (Invitrogen) for 20 minutes ...
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bioRxiv - Bioengineering 2021Quote: ... Quality and quantity of extracted RNA was verified by NanoDrop spectrophotometry prior to implementation of the 1 step RNA to Ct kit (ThermoFisher). Each measurement was conducted in triplicate with non-template controls using a BioRad CFX96 instrument ...
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bioRxiv - Neuroscience 2021Quote: The Q205L mutation was engineered into goa-1::gfp gene pMK376 using the GeneArt Site-Directed Mutagenesis PLUS Kit (Invitrogen) and the primers SKB48 (5’-tgtgggaggtctgagatcagaaag-3’ ...
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bioRxiv - Cell Biology 2021Quote: ... 1 μg of total RNA was used to transcribe cDNA using a high-capacity RNA-to-cDNA kit (Applied Biosystems) per the manufacturer’s protocol ...
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bioRxiv - Neuroscience 2020Quote: ... Complementary DNA from 1 µg of input RNA was generated with the High Capacity cDNA Reverse Transcription Kits (Life Technologies). SYBR Green PCR master mix (Life Technologies ...
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bioRxiv - Biochemistry 2021Quote: ... 1 µg of RNA was used for cDNA synthesis (First Strand cDNA Synthesis Kit, Thermo Fisher Scientific, Waltham, MA, USA). Another 30 mg aliquot of lung powder was transferred into RIPA buffer ...
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bioRxiv - Microbiology 2020Quote: ... T4 phage were added (3×108 phages ml-1) to the AFM bio-cell together with SYTO 9 and PI (BacLight Bacterial Viability and Counting Kit, Invitrogen, ThermoFisher Scientific ...
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bioRxiv - Microbiology 2021Quote: ... the extracted DNA samples were checked for quality and quantity by a 1% agarose gel (Midori Green-stained) electrophoresis and a Qubit dsDNA HS Assay Kit (Invitrogen). Prior to library preparation ...
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bioRxiv - Plant Biology 2020Quote: ... cDNA was synthesized from 1 ug of RNA using the High-Capacity cDNA Reverse Transcription Kit (Thermo Fisher Scientific 4368814). RT-qPCR analysis was performed with iQ SYBR Green Supermix in an iCycler iQ5 system (Bio-Rad ...
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bioRxiv - Microbiology 2021Quote: ... and placed in a prefilled bead tube containing 1.00mm glass beads (Beadbug, Z763756) and 600μl of lysis buffer with 1% BME from the Purelink RNA mini kit (Invitrogen, USA). The tissue was homogenized for 1 minute disruption at 5 m/s and 1 min cooling in ice ...
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bioRxiv - Neuroscience 2020Quote: ... cDNA was then synthesized from 1 μg RNA using Superscript III First Strand Synthesis kit (Invitrogen; #18080051, San Diego, CA). Amplification of target sequences was performed using TaqMan Fast Advanced Master Mix (Applied Biosystems ...
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bioRxiv - Microbiology 2020Quote: 0.5-1 µg total RNA was prepared for first-strand cDNA synthesis using the superscript IV synthesis kit (Invitrogen, USA) and quantitative real-time RT-PCR (qRT-PCR ...
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bioRxiv - Neuroscience 2020Quote: ... Barcoding was performed with the indices from the Ion Xpress™ RNA-Seq Barcode 1-16 Kit (Life Technologies 4471250) according to the manufacturer’s protocol and as previously published28 ...
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bioRxiv - Biophysics 2020Quote: Expression was conducted according to the manual of 1-Step Human High-Yield Mini in vitro translation (IVT) kit (Product No. 88891X) distributed by ThermoFisher Scientific (Pierce Biotechnology ...