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Citations for Thermo Fisher :
7301 - 7350 of 10000+ citations for Glutathione Reductase Fluorescent Kit 1 Plate since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2021Quote: ... BBB organoids were labelled with calcein-AM and ethidium homodimer-1 according to the manufacturer’s protocol (Live/DEAD Viability/Cytotoxicity Kit for mammalian cells, ThermoFisher Scientific) and were imaged using a a Nikon Eclipse Ti-E fully motorized microscope (Nikon).
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bioRxiv - Molecular Biology 2022Quote: ... 1 µg of total RNA was used for cDNA synthesis using the Maxima First Strand cDNA kit (Thermo Fisher Scientific). qPCR assays were conducted using Taq platinum (Invitrogen ...
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bioRxiv - Immunology 2022Quote: ... Reverse transcription of 1 μg of total RNA was performed using the SuperScript VILO cDNA Synthesis kit (Thermo Fisher Scientific). Quantitative real-time PCR (qRT-PCR ...
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bioRxiv - Cell Biology 2022Quote: ... Dead cells were excluded using LIVE/DEAD™ Fixable Near-IR Dead Cell Stain Kit (ThermoFisher Scientific, Dilution: 1/1500). Data were analysed using Flowjo v10.
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bioRxiv - Systems Biology 2022Quote: ... Reverse transcription with random primers and subsequent PCR were performed with TaqMan RNA-to-CT 1-Step Kit (Applied Biosystems). Raw data for cycle threshold (Ct ...
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bioRxiv - Bioengineering 2022Quote: ... were cultured in T75 flasks and maintained in complete HUVEC media (VascuLife VEGF Endothelial Medium Complete Kit) supplemented with 1% penicillin/streptomycin (Gibco). HUVEC media was changed every 3 days until cells reached 90% confluency ...
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bioRxiv - Genomics 2022Quote: We diluted the RNA samples to 1 ng/μL for library preparation and confirmed the concentration using a Qubit RNA High Sensitivity Assay Kit (Invitrogen™ ...
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bioRxiv - Microbiology 2022Quote: ... SARS-CoV-2 RNA was detected with the CDC assay kit (IDT) and the TaqMan Fast Virus 1-Step Master Mix (ThermoFisher) on the QuantStudio3 System (Invitrogen).
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bioRxiv - Microbiology 2022Quote: ... A total of 1 µg of DNA was used for in vitro transcription using mMESSAGE mMACHINE T7 Ultra Transcription kit (Invitrogen) with the 5′ end capped with anti-reverse cap analog (ARCA ...
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bioRxiv - Microbiology 2022Quote: ... cDNA was generated using 1 μg of DNAase-treated RNA with the High-Capacity cDNA Reverse Transcription Kit (Applied Biosystems) and kit protocol ...
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bioRxiv - Microbiology 2020Quote: ... consisting of lysis enhancer and resuspension buffer at a 1:10 ratio (both from the CellsDirect One-Step RT-qPCR kit; Invitrogen). Next ...
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bioRxiv - Molecular Biology 2021Quote: ... 0.5–1 μg of RNA were reverse-transcribed to cDNA using the High Capacity cDNA Reverse Transcription Kit (Applied Biosystems) according to the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2020Quote: ... cDNA was prepared using 1 μg RNA and the High-Capacity cDNA Reverse Transcription Kit (Applied Biosystems, Catalog number: 4368814). The qPCR reactions were performed using TaqMan Gene Expression Master Mix (Applied Biosystems ...
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bioRxiv - Cell Biology 2021Quote: ... cDNA of 1000 ng µL-1 was obtained using the High-Capacity cDNA Reverse Transcription Kit (ThermoFisher Scientific, MA, USA), and the absence of DNA contamination was verified by excluding the reverse transcriptase from subsequent PCR reaction ...
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bioRxiv - Cell Biology 2021Quote: NRVMs were derived from 1- or 2-day old Sprague-Dawley rates using the Cellutron Neomyt kit (Thermo Fisher Scientific). Cell purity was routinely >90% cardiomyocytes ...
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bioRxiv - Cancer Biology 2020Quote: ... organoids were incubated with 2 mM Calcein-AM and 4 mM Ethidium homodimer-1 (LIVE/DEAD assay kit, Molecular Probes) for up to 6h ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... RNA (1 μg) was reverse transcribed using the High Capacity cDNA Reverse Transcription kit (Applied Biosystems, Foster City, CA, USA). Quantitative PCR assays were performed using the CFX96 Real Time System (Bio-Rad ...
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bioRxiv - Neuroscience 2022Quote: ... followed by cDNA preparation from 0.5–1 μg total RNA using the High Capacity cDNA Reverse Transcription kit (Applied Biosystems). The resulting cDNA samples were diluted ...
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bioRxiv - Evolutionary Biology 2022Quote: ... Pooled DNA was separated on a 1% TAE-agarose gel and desired band extracted using PureLink Quick Gel Extraction Kit (Invitrogen). The extracted DNA was cleaned and concentrated using a DNA Clean & Concentrator Kit (Zymo ...
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bioRxiv - Plant Biology 2022Quote: ... Samples were then treated with Deoxyribonuclease I and 1 μg of total RNA was used for cDNA synthesis using the SuperScript III First-Strand synthesis system kit (Invitrogen).
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bioRxiv - Immunology 2019Quote: ... The gag-protease region was amplified by reverse transcription (RT)-PCR from plasma HIV-1 RNA using Superscript III One-Step Reverse Transcriptase kit (Invitrogen) and the following gag-protease -specific primers ...
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bioRxiv - Cancer Biology 2019Quote: ... KRASWT and KRASHRAS transcript abundances were quantified by RT-qPCR (Power SYBR Green RNA-to-CT 1-Step Kit, ThermoFisher). Primers for FLAG-KRASWT amplification ...
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bioRxiv - Genomics 2019Quote: ... 1 million PBMCs were plated in medium supplemented with four OSKM reprogramming factors (CytoTune-iPS Sendai Reprogramming Kit, Life Technologies) according to the manufacturer’s recommendations ...
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bioRxiv - Molecular Biology 2019Quote: ... ama-1 was cloned into pJET 1.2/blunt cloning vector using CloneJET PCR Cloning Kit (Thermo scientific, catalogue number K1232). The cloned plasmid was amplified in DH5-Alpha cells and purified with QIAfilter™ Plasmid Maxi Kit (Qiagen ...
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bioRxiv - Physiology 2019Quote: GM-1 clusters were labeled using a Vybrant® Alexa Fluor® 488 Lipid Raft Labeling Kit (ThermoFisher Scientific: V34404) as previously described (69) ...
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bioRxiv - Physiology 2019Quote: ... Endogenous peroxidase activity was blocked with 3% hydrogen peroxide in phosphate-buffered saline (PBS) followed by an additional blocking step with 1% Tyramide Signal Amplification (TSA) blocking reagent (TSA kit, T20935, Invitrogen) and Mouse-on-Mouse blocking reagent (Vector Laboratories ...
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bioRxiv - Pathology 2020Quote: ... complementary DNA (cDNA) was synthesized from 1 μg of total RNA using the Verso® cDNA Synthesis Kit (ThermoFisher Scientific). The partial sequence encoding the nucleoprotein from HPWMoV was amplified using specific primers (Table 1 ...
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bioRxiv - Microbiology 2020Quote: ... Equal amounts of untreated or RNase R-treated RNA (1 µg) were used in reverse transcription reactions using the High Capacity RNA-to-cDNA Kit (Invitrogen), followed by qPCR reactions using SYBR® Green PCR Master Mix (Invitrogen).
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bioRxiv - Neuroscience 2020Quote: ... RNA samples (1 μg each) were then reverse-transcribed into cDNA using a SuperScript III First-Strand Synthesis kit (Invitrogen) according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2021Quote: ... of THP-1 monocytes +/− ML-60218 exposure and THP-1 macrophages (72 h PMA) using the mirVana miRNA isolation kit according to instructions (AM1560, Invitrogen). Nuclear and cytoplasmic THP-1 fractions were isolated using the NE-PER Nuclear and Cytoplasmic Extraction Reagents (ThermoFisher ...
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bioRxiv - Molecular Biology 2021Quote: ... Human globin mRNA was depleted from all samples except from samples #1 and #2 using the GLOBINclear kit (ThermoFisher Scientific). The quality of the RNA was assessed using the Agilent 6000 Pico kit with the Bioanalyzer 2100 (Agilent ...
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bioRxiv - Physiology 2021Quote: ... In-situ hybridization imaging of RAGE was conducted using the QuantiGene ViewRNA ISH Tissue 1-plex Assay kit and procedure from Affymetrix. We performed the assay using a modified 1-plex protocol for Fast Blue Detection using Type 1 Mouse AGER probe and Type 1 Mouse GAPDH probe (Affymetrix) ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... the treated and untreated Jurkat cells were washed with 1×PBS once before staining with propidium iodide (PI) cell stain kit (Invitrogen) and Hoechst 33342 (Cell Signaling Technology ...
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bioRxiv - Physiology 2020Quote: ... cDNA synthesis was performed with 1 μg of total RNA using the MultiScribe™ Reverse Transcriptase Kit (Thermo Fisher Scientific). Primers were designed from cavia porcellus sequences available in Ensembl or NCBI databases using the NCBI/Primer-BLAST tool ...
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bioRxiv - Molecular Biology 2021Quote: ... RNA interference plasmids were built using annealed DNA oligonucleotides (Supplementary Note 1) in the pcDNA6.2-GW/EmGFP plasmid (Block-iT™ miRNA expression kit from Invitrogen). Specific primers for pre-miRNA were designed using Life Technologies’ online tool (www.lifetechnologies.com/rnai) ...
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bioRxiv - Molecular Biology 2021Quote: ... 1 μL rDNase I and 2 μL DNase I Buffer from DNase Treatment and Removal Reagents Kit (ThermoFisher Scientific AM1906) were added to each RNA sample and then incubated at 37°C for 30 min ...
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bioRxiv - Developmental Biology 2019Quote: ... The ERlucT-IRES-Rluc bicistronic expression cassette was then PCR amplified using primers listed in Table 1 and then subcloned using the pCR8/GW/TOPO TA Cloning Kit (Invitrogen), to produce the Gateway cloning-compatible ERlucT-IRES-Rluc-pCR8 middle entry vector ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... and cDNA was prepared from 0.5–1 μg total RNA using the High Capacity cDNA Reverse Transcription kit (Applied Biosystems). The resulting cDNA samples were amplified using the iQ SYBR Green Supermix (Biorad ...
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bioRxiv - Cell Biology 2020Quote: 1 µg of total RNA was used to synthesize cDNA using SuperScript III RT First Strand cDNA Synthesis Kit (Invitrogen). Real-time PCRs were performed on an ABI7900HT system ...
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bioRxiv - Immunology 2020Quote: ... and detection antibody against mouse IgA (1 in 250) from the mouse total IgA ELISA kit from Invitrogen (CA, USA). The positive control (anti-S IgG ...
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bioRxiv - Biochemistry 2020Quote: ... The pellet was re-suspended in 5mL of 1% SDC + 50mM ABC pH 8.5 and protein concentration was measured with Pierce BCA protein assay kit (Thermo Scientific). Samples were then aliquoted into 1.5mL LoBind eppendorf tubes (Sorenson BioScience ...
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bioRxiv - Genomics 2020Quote: ... Genomic DNA was extracted from 1 ml liquid cultures using the PureLink® Genomic DNA Mini Kit (Thermo Fisher Scientific) according to manual instructions ...
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bioRxiv - Cell Biology 2021Quote: ... the 1 ng of RNA was reverse-transcribed into cDNA using a Verso cDNA Synthesis Kit (Thermo Scientific, Lithuania, USA). The cDNA was used for qRT-PCR to quantify the expression of 20 genes (Table 3 ...
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bioRxiv - Biochemistry 2021Quote: RNA was isolated from the aqueous phase of homogenized spleens mixed with 1-bromo-3-chloropropane and purified with the PureLink RNA kit (Invitrogen) separately ...
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bioRxiv - Molecular Biology 2021Quote: ... Reverse transcription with random primers and subsequent PCR were performed with TaqMan RNA-to-CT 1-Step Kit (Applied Biosystems). All experimental groups were assessed as 6 technical replicates and repeated at least three times.
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bioRxiv - Cell Biology 2021Quote: ... The cDNA was generated using 1 μg of total RNA using the kit First-Strand cDNA Synthesis (Thermo Scientific # 18080051) using oligoDT and random hexamer primers ...
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bioRxiv - Cell Biology 2021Quote: ... HeLa and BJ 1-2 μg of total cell RNA was reverse transcribed into cDNA using SuperScript VILO cDNA Synthesis Kit (11754050, ThermoFisher). A volume corresponding to 25-50 ng of cDNA was used for each RT-qPCR reaction using Roche LightCycler 480 SYBR Green I Master (04707516001 ...
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bioRxiv - Developmental Biology 2021Quote: ... 0.5–1 µg of RNA was reverse transcribed to cDNA using the High Capacity cDNA Reverse Transcription Kit (Applied Biosystems) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2021Quote: ... was synthesized from isolated RNA (1 µg) using the SuperScript III First-Strand Synthesis System for RT-PCR kit (Invitrogen), following the manufacturer’s instructions ...
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bioRxiv - Microbiology 2020Quote: RNA was extracted from 47 mm flat filter halves from all three expeditions (2012, 2014, and 2017, Table 1) using a modified version of the mirVana RNA extraction kit (Ambion) protocol (Supplemental Methods) ...