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Citations for Thermo Fisher :
7101 - 7150 of 10000+ citations since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Plant Biology 2024Quote: ... PBS with 1 µg/ml WGA-Alexa Fluor 488 (Invitrogen) overnight at 4°C ...
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bioRxiv - Plant Biology 2024Quote: ... Total RNA was isolated following the TRIzol manufacturer’s instructions (Invitrogen) and quantified using a BioPhotometer Plus (Eppendorf AG ...
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bioRxiv - Plant Biology 2024Quote: ... 10 ml SUPERase-In (Invitrogen Cat# AM2694) RNase inhibitor was then added to stop nuclease digestion ...
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bioRxiv - Plant Biology 2024Quote: ... The concentration of DNA library was measured by Qubit (Invitrogen). Libraries were sequenced by an Illumina NovaSeq sequencer (Genergy Biotechnology Shanghai ...
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bioRxiv - Plant Biology 2024Quote: ... using the 2X PowerUp SYBR green master mix (Applied Biosystems, ThermoFisher Scientific) with the following settings ...
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bioRxiv - Plant Biology 2024Quote: ... PPR ORFs were cloned into the pDONR207 vector using Gateway BP clonase enzyme (Invitrogen), then subcloned using Gateway LR clonase enzyme (Invitrogen ...
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bioRxiv - Plant Biology 2024Quote: ... using the 2X PowerUp SYBR green master mix (Applied Biosystems, ThermoFisher Scientific) with the following settings ...
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bioRxiv - Plant Biology 2024Quote: Using LR reactions (Invitrogen), the plasmids pENTR L4-promoter-R1 ...
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bioRxiv - Plant Biology 2024Quote: ... The purity of all RNA samples was confirmed using a NanoDrop2000 instrument (Thermo Scientific). The 3’RNA-seq libraries were prepared from 6 µg total RNA at the Cornell Genomics facility (http://www.biotech.cornell.edu/brc/genomics-facility ...
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bioRxiv - Plant Biology 2024Quote: ... coli cells (Invitrogen). The expression vector encoding 6xHis-MBP-OsHIPP43 was transformed into E ...
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bioRxiv - Plant Biology 2024Quote: ... in a QuantStudio5 machine (Applied Biosystems). Transcripts were amplified and results were normalized to PP2A transcript levels ...
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bioRxiv - Biochemistry 2024Quote: ... BCA assay (Thermo Fisher Scientific) was then used to quantify protein yields and aliquots of 100 μg were subjected to click reaction with TAMRA-N3 (25 μM TAMRA-N3 (BroadPharm) ...
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bioRxiv - Biochemistry 2024Quote: ... cell suspensions were incubated for 20 min at 4°C in 80 µL of L15 medium containing 2.1 µg of anti-rat CD2 antibody (Invitrogen) per 15 million dissociated cells ...
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bioRxiv - Biochemistry 2024Quote: ... 2 mM L-glutamine (Gibco), 100 Units/mL penicillin (Gibco) ...
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bioRxiv - Biochemistry 2024Quote: ... The next day cells were trypsinized and resuspended in optiMEM (no phenol red, Gibco) at the density of 2 x 105 cells/mL ...
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bioRxiv - Biochemistry 2024Quote: Protein cages were packed into liposomes using Lipofectamine CRISPRmax Cas9 transfection reagents (Invitrogen). Protein cages were incubated with Lipofectamine CRISPRmax transfection reagents for 30 minutes in opti-MEM then added dropwise to cells ...
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bioRxiv - Biochemistry 2024Quote: ... The proteins were expressed overnight at 18°C in Terrific Broth (Thermo Scientific) and 0.4% v/v glycerol with 0.5 mM isopropyl ß-D-1-thiogalactopyranoside (IPTG ...
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bioRxiv - Biochemistry 2024Quote: Protein cages were labeled with AF647 Antibody Labeling Kits (Invitrogen). Over 500 µg of protein cage was incubated per reaction for 1 hour at 25°C with periodic inversion ...
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bioRxiv - Biochemistry 2024Quote: ... cells were heated in the VerityPro thermocycler (Thermo Fisher Scientific) as follows ...
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bioRxiv - Biochemistry 2024Quote: ... 1X EDTA-free protease inhibitor cocktail (Thermo Scientific), and 0.1% LDAO ...
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bioRxiv - Biochemistry 2024Quote: ... Coverslips were then mounted with ProLong Glass Antifade Mountant (Thermo Scientific) on microscope slides ...
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bioRxiv - Plant Biology 2024Quote: ... the cells were incubated with the 10 μM H2DCFDA (21,71-dichlorfluorescein-diacetate; Thermo Fisher Scientific ...
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bioRxiv - Plant Biology 2024Quote: ... the corresponding AtPIN5 open reading frame was cloned with the Gateway system (Invitrogen, Thermo Fisher Scientific, Waltham, MA, USA) into the pMDC7 vector (Curtis & Grossniklaus ...
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bioRxiv - Plant Biology 2024Quote: ... the corresponding AtPIN5 open reading frame was cloned with the Gateway system (Invitrogen, Thermo Fisher Scientific ...
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bioRxiv - Plant Biology 2024Quote: SAMs dissected in the net house were immediately transferred to 300 μl RNAlater™ solution (Thermo Fisher Scientific, USA) for storage at -18°C ...
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bioRxiv - Bioengineering 2024Quote: ... and mounted with ProLong Diamond antifade mountant (Invitrogen, cat. no. 36961). Samples were visualized using a scanning laser confocal microscope (A1 confocal system with Eclipse Ti inverted microscope ...
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bioRxiv - Plant Biology 2024Quote: ... and recombined into the Gateway destination vector pEarleyGate100 (pEG100) (Earley et al., 2006; Helm et al., 2022) using LR Clonase II (Invitrogen), thereby generating C- terminal-tagged effector-fluorescent protein fusions.
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bioRxiv - Plant Biology 2024Quote: ... The fluorescent POD staining reaction was performed with Tyramide Signal Amplification Kit (Fisher Scientific, Cat No: 15611892) according to (Rozier et al. ...
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bioRxiv - Plant Biology 2024Quote: ... was used for cDNAs synthesis with using the High-Capacity cDNA Reverse Transcription kit (Applied Biosystems) with DNAse I treatment (Roche) ...
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bioRxiv - Plant Biology 2024Quote: ... oligo d(T)20 primers (Invitrogen) and 1 µg of RNA in a final reaction volume of 20 µl.
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bioRxiv - Plant Biology 2024Quote: ... and treated with DNA-Free kit (Thermo Fisher Scientific). RNA purity ...
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bioRxiv - Biochemistry 2024Quote: ... a protease inhibitor cocktail (Thermo Scientific) was added to the resuspended bacterial pellet and cell lysis was carried out by sonication (probe-tip sonicator Branson) ...
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bioRxiv - Biochemistry 2024Quote: ... A Flp-In 293 cell line containing a single stably integrated FRT site at a transcriptionally active genomic locus was purchased from Invitrogen (R75007, Thermo Fisher Scientific). The FDA approved drug library was from APExBIO (L1021 ...
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bioRxiv - Biochemistry 2024Quote: ... Purified pOG44 and pcDNA5/FRT-SUGCT plasmids were co-transfected into Flp-In 293 cells at a 9:1 ratio by using Lipofectamine 2000 reagent (Invitrogen, Thermo Fisher Scientific). Flp-In 293 cells were cultured in DMEM supplemented with 10% FBS ...
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bioRxiv - Biochemistry 2024Quote: ... and transformed to TOP10 chemically competent cells (Thermo Fisher Scientific). Successful cloning was confirmed by sequencing ...
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bioRxiv - Biochemistry 2024Quote: ... A Flp-In 293 cell line containing a single stably integrated FRT site at a transcriptionally active genomic locus was purchased from Invitrogen (R75007, Thermo Fisher Scientific). The FDA approved drug library was from APExBIO (L1021 ...
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bioRxiv - Biochemistry 2024Quote: ... Purified pOG44 and pcDNA5/FRT-SUGCT plasmids were co-transfected into Flp-In 293 cells at a 9:1 ratio by using Lipofectamine 2000 reagent (Invitrogen, Thermo Fisher Scientific). Flp-In 293 cells were cultured in DMEM supplemented with 10% FBS ...
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bioRxiv - Biochemistry 2024Quote: ... goat anti-mouse Alexa Fluor 596 (ThermoFisher Scientific, dilution IF: 1’000), goat anti-rabbit IRDye 800CW conju-gated (LI-COR Biosciences ...
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bioRxiv - Biochemistry 2024Quote: ... Luminescence was detected using VarioskanTM Flash multimode reader (Thermo Scientific). A standard curve for ATP was prepared with differing concentrations of ATP according to the manufacturer’s protocol.
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bioRxiv - Plant Biology 2024Quote: Total RNA was isolated from quinoa leaves using TRIzol Reagent (Invitrogen) following the manufacturer’s instructions ...
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bioRxiv - Plant Biology 2024Quote: ... All protein samples were exchanged into the same buffer prior to each experiment via overnight dialysis in Slide-A-Lyzer MINI Dialysis Devices (Thermo Scientific). The Pwl effectors were placed in the experimental cell at 20 μM and titrated with OsHIPP43 at 200-300 μM at 25 °C ...
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bioRxiv - Plant Biology 2024Quote: ... and converted to cDNA with a SuperScript IV First-Strand Synthesis System (Thermo Fisher, USA). Primers were designed for CcSOC1a ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... For the final 30 minutes of staining LIVE/DEAD yellow (1:1000) (Thermo Scientific L34967) was added to cell mixtures ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... CD11c rat anti-mouse Super Bright 780 (Invitrogen - 78-0114-82), CD3 anti-mouse PE (Invitrogen - 12-0032-82) ...
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bioRxiv - Plant Biology 2024Quote: ... The resulting fragment was introduced into the vector pDONR221 (Thermo Scientific) by carrying out a BP reaction ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... a Rat bFGF ELISA Kit (Invitrogen, CA), and an AssayMax™ Dihydrotestosterone ELISA Kit (Assaypro ...
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bioRxiv - Physiology 2024Quote: ... Membranes were washed and developed using the ECL method (Thermo Fisher Scientific, Waltham, MA 34577). Imaging was performed on a Syngene GBOX Chemi XX6 (Syngene ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... grew as a mixture of floating and adherent cells in DMEM/F-12 (Gibco) medium supplemented with 1% penicillin/streptomycin (Sigma-Aldrich) ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... 2 mM GlutaMax (Gibco), 0,5 % FBS (BioChrom AG ...
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bioRxiv - Biochemistry 2024Quote: Peptide identification and TMT-based protein quantification was performed in Proteome Discoverer 2.4 (PD, Thermo Fisher). MS/MS spectra were searched using SEQUEST-HT against a Uniprot human proteome database (released 03/2014 and containing 20,337 entries ...