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Citations for Thermo Fisher :
7301 - 7350 of 10000+ citations since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Bioengineering 2024Quote: ... at 20-25V for 7 minutes using the iBlot Gel Transfer Device and transfer stacks (Invitrogen).
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bioRxiv - Bioengineering 2024Quote: ... Membranes were washed and stained overnight at 4°C with anti-PODXL (Invitrogen, PA5-28116, 1:1000 concentration), anti-CD63 (Abcam ...
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bioRxiv - Bioengineering 2024Quote: ... Electrophoresis was perfomed in Bolt MOPS SDS Running Buffer (Invitrogen) at 200V for 35 minutes or until protein front reached the bottom of gels ...
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bioRxiv - Plant Biology 2024Quote: ... Library size and concentration were determined using Agilent 2100 Bioanalyzer and Qubit (Thermo Fisher Scientific), respectively ...
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bioRxiv - Plant Biology 2024Quote: ... as the primary antibody at 1:100 dilution and donkey anti-rabbit AlexaFluor 555 antibodies (A31572, Thermo Fisher Scientific, Waltham, MA) as the secondary antibody at 1:1000 dilution ...
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bioRxiv - Plant Biology 2024Quote: ... A Microm HM525 cryostat (Thermo Fisher, Waltham, MA, USA) was used for making 16 µm thick sections ...
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bioRxiv - Plant Biology 2024Quote: LC-MS/MS was carried out on a Q-Exactive HF hybrid quadrupole-Orbitrap mass spectrometer (Thermo Fisher Scientific), equipped with an Easy LC 1200 UPLC liquid chromatography system (Thermo Fisher Scientific) ...
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bioRxiv - Plant Biology 2024Quote: ... 1:100 dilution) as the primary antibody and donkey anti-mouse AlexaFluor 488 antibodies (A21202, Thermo Fisher Scientific ...
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bioRxiv - Plant Biology 2024Quote: ... and centrifuged at 4700 g for 30 min before loading the supernatant on a HisPur Ni-NTA Spin Column (Thermo Fisher). The column with cleared lysate was rotated for 1 h after which it was washed three times with washing buffer (50 mM Tris-HCl pH 7.5 ...
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bioRxiv - Plant Biology 2024Quote: ... Quality assessment was conducted using a NanoDrop ONE spectrophotometer (Thermo Fisher Scientific, USA). RNA samples were checked for any degradation on 1.2% agarose gels ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... CD19 anti-mouse Brilliant Ultra Violet 805 (Invitrogen – 368-0193-82), CD14 anti mouse PerCP-710 (Invitrogen – 46-0141-82 ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... After 24 hours we added the differentiation medium containing Dulbecco’s modified eagle medium DMEM/F12 without phenol red (#21041025, Thermo Fisher), supplemented with 1% penicillin/streptomycin (Sigma-Aldrich) ...
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bioRxiv - Physiology 2024Quote: ... One-half μg of RNA was converted to cDNA using the Verso cDNA synthesis kit (Thermo Fisher Scientific, Waltham, MA). PCR amplification of the cDNA was performed by quantitative real-time PCR using TrueAmp SYBR Green qPCR SuperMix (Smart Bioscience ...
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bioRxiv - Plant Biology 2024Quote: ... were integrated into the pENTRTM/D-TOPOTM vector using the pENTRTM/D-TOPOTM cloning kit (Thermo Fisher), and subsequently transferred into the pGWB435 Gateway-compatible destination vector (Nakagawa et al. ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... CD8a anti-mouse APC (Invitrogen – APC-65069-100UG), CD19 anti-mouse Brilliant Ultra Violet 805 (Invitrogen – 368-0193-82) ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: pcDNA4TO-TS-SNAP-β2AR or pcDNA4TO-TS-SNAP-β2AR-nLuc were stably transfected into T-RexTM-293 cells (Invitrogen) using polyethylenimine (PEI) ...
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bioRxiv - Bioengineering 2024Quote: Detection was performed with the 1-Step™ TMB Substrate Kit (Thermo Fisher Scientific, cat.: 34021) and quenched with 3M H2SO4 ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... T75 and T175 mammalian cell culture flasks were purchased from Fisher scientific (Loughborough, UK). All cell culture reagents ...
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bioRxiv - Physiology 2024Quote: ... rabbit anti-SAP30 (Invitrogen PA5-103284 ...
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bioRxiv - Physiology 2024Quote: ... F-Actin was stained with Alexa Fluor 568 phalloidin (1:200; Invitrogen, #A12380). For DAPI and phalloidin staining ...
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bioRxiv - Physiology 2024Quote: RNA-seq was performed at the DNA Core at the CCHMC facility with 10 ng – 150 ng of total RNA used after quantification by Qubit RNA HS assay kit (Cat #Q32852; Invitrogen, Waltham, MA). Based RNA integrity value above 7 determined by the spectrofluorometric measurement RNA samples was poly-A selected and reverse transcribed using Illumina’s TruSeq stranded mRNA library preparation kit (Cat# 20020595 ...
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bioRxiv - Physiology 2024Quote: ... rabbit anti-ARID5a (Invitrogen MA518292), rabbit anti-Phospho IRS (S307 ...
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bioRxiv - Physiology 2024Quote: ... The male reproductive tracts were dissected in Schneider’s Drosophila Medium (SDM; Thermo Fisher Scientific, #21720024), and male accessory glands were removed from the male reproductive tracts using forceps ...
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bioRxiv - Physiology 2024Quote: The separation of nuclear and cytoplasmic protein analysis was performed using NE-PER nuclear and cytoplasmic extraction kit (Invitrogen #78835). Briefly ...
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bioRxiv - Physiology 2024Quote: ... The tissue sample was cut into pieces using a sterile blade and transferred to a 2 ml Eppendorf tube to which 1ml of Trizol reagent (Invitrogen # 15596018) was added ...
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bioRxiv - Plant Biology 2024Quote: Approximately 2,000 seeds of Arabidopsis thaliana wild type accession Columbia (Col-0) were surface sterilized using a solution of 30% household bleach supplemented with 0.1% Triton X-100 (Thermo Scientific. Catalog number: A16046-AE) for 5 minutes ...
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bioRxiv - Physiology 2024Quote: ... the detection of chemiluminescence was achieved by incubation of the membrane with a substrate such as SuperSignal™ West Femto Maximum Sensitivity Substrate (34094, Thermo Scientific) or SuperSignal™ West Pico PLUS Chemiluminescent Substrate (34577 ...
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bioRxiv - Physiology 2024Quote: ... following quantification using Qubit ds DNA quantification assay kit (Invitrogen #Q32851) was done and DNA concentration of 1ng was taken for analysis ...
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bioRxiv - Physiology 2024Quote: ... rabbit anti-PPARG (Invitrogen PA3-821A), rabbit anti-SAP30 (Invitrogen PA5-103284 ...
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bioRxiv - Physiology 2024Quote: Excised muscle tissues were fixed in 10% formaldehyde (Cat #245-684; Fisher Scientific, Waltham, MA) at room temperature for ∼24 hours ...
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bioRxiv - Physiology 2024Quote: ... Pierce A/G magnetic beads (Invitrogen # 80105G) of 30 µl were washed using FA lysis buffer 2 times and mixed with the IP samples ...
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bioRxiv - Physiology 2024Quote: ... or SuperSignal™ West Pico PLUS Chemiluminescent Substrate (34577, Thermo Scientific). The substrate was removed ...
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bioRxiv - Physiology 2024Quote: ... followed by incubation with fluorophore (Alexa Fluor 488)-conjugated secondary antibodies (Thermo Fisher Scientific; 1:200) and in blocking solution for 2 hr at RT or overnight at 4 °C ...
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bioRxiv - Physiology 2024Quote: Immunoprecipitation of proteins without the contaminant of antibody heavy and light chain through bead antibody conjugation was carried out using the Pierce Co-IP kit (Invitrogen #26149). Briefly ...
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bioRxiv - Plant Biology 2024Quote: ... DNA samples were quantified using a Qubit 2.0 Fluorometer (Invitrogen, Carlsbad, CA, USA) and 30-50 ng of DNA were used to generate amplicons using a MetaVx™ Library Preparation kit (GENEWIZ ...
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bioRxiv - Physiology 2024Quote: ... AlexaFluor488 anti-IgG1 and AlexaFluor594 anti-IgM (Cat #A21140, A21121, 1010111; Life Technologies, Grand Island, NY). Type 1 fibers stained blue ...
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bioRxiv - Physiology 2024Quote: ... Cells were stained with LIVE/DEAD Yellow (1:1000) (Thermo Scientific L34967) for 30 minutes on ice ...
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bioRxiv - Physiology 2024Quote: ... or 1:1,000 Alexa fluor 555 goat anti Rabbit IgG (H+L) (Life Technologies, cat#A-A21428) respectively ...
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bioRxiv - Physiology 2024Quote: ... the total amount of soluble protein in each sample was measured with a BCA Assay Kit (Thermo Scientific) according to manufacturer’s instructions ...
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bioRxiv - Physiology 2024Quote: ... Nuclei were stained with 1:2,000 propidium iodide (Invitrogen, cat#P21493). All immunofluorescence images were captured using a confocal microscope (Olympus FV1000).
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bioRxiv - Physiology 2024Quote: ... Sections were washed (3xPBS) and counterstained with DAPI (Thermo Fisher Scientific D3571) before mounting ...
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bioRxiv - Physiology 2024Quote: ... 1:1,000 Alexa fluor 568 goat anti Mouse IgG2b (H+L) (Life Technologies, cat#A-A21144) or 1:1,000 Alexa fluor 555 goat anti Rabbit IgG (H+L ...
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bioRxiv - Physiology 2024Quote: ... 0.5 mg/ml rhodamine-dextran (Life Technologies, 10 kDa, neutral) in 20 µl Ringer’s solution was added mucosally ...
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bioRxiv - Physiology 2024Quote: ... during primary incubation and DAPI (1:500 - Thermo Fisher D3571) and Wheat-Germ-Agglutinin-Alexa Fluor 647 (5 μg/m – Thermo Fisher W32466 ...
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bioRxiv - Physiology 2024Quote: ... Sections were incubated with secondary antibody 1:1,000 Alexa fluor 488 goat anti Rabbit IgG (H+L) (Invitrogen, cat# A-11008) 1 hr at room temperature ...
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bioRxiv - Physiology 2024Quote: ... Intracellular Ca2+ ([Ca2+]i) measurements were conducted using Fluo-4/AM (5 µM; Invitrogen)-loaded cells ...
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bioRxiv - Physiology 2024Quote: ... and incubated with secondary antibody 1:1,000 Alexa fluor 555 goat anti Rabbit IgG (H+L) (Life Technologies, cat#A-A21428), 1:1,000 Alexa fluor 568 goat anti Mouse IgG2b (H+L ...
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bioRxiv - Physiology 2024Quote: ... with cDNA prepared using a SuperScript™ VILO™ cDNA synthesis kit (Invitrogen, #11756050). RT-qPCR using TaqMan assays (Applied Biosystems ...
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bioRxiv - Physiology 2024Quote: ... The samples were filtered through a silicon membrane (1 μm retention limit, Microplastic Sample Preparation Kit, Thermo Fisher Nepean, ON) using glass vacuum filtration funnel ...
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bioRxiv - Physiology 2024Quote: ... Slides were covered with ProLong™ Gold Antifade Mountant (Invitrogen P36930), a coverslip ...