Labshake search
Citations for Thermo Fisher :
651 - 700 of 10000+ citations for Recombinant Human Collagen Type II Alpha 1 His tagged since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Pathology 2024Quote: ... The adventitial membrane was chopped to the size of 1-2mm2 and placed directly into serum-free low-glycemic medium containing 2mg/ml type II collagenase (Cat#17101015, Thermo Fisher Scientific) for digestion for about 4 hours ...
-
bioRxiv - Cancer Biology 2019Quote: ... bait GST-tagged EIF4E or 6xHis-tagged CELF1 proteins (1 μM) were initially bound to glutathione-agarose beads (ThermoFisher Scientific) or Ni2+ beads (GE Healthcare) ...
-
bioRxiv - Immunology 2019Quote: ... containing 1 mg/ml collagenase Type 1 (Life Technologies), 0.4 units Dispase (Life Technologies) ...
-
bioRxiv - Cell Biology 2022Quote: ... The His SNX32ΔC protein bound to the lipids was detected by anti-His antibody [1:10,000 (Thermo Scientific)]
-
bioRxiv - Immunology 2020Quote: ... Primary antibodies against the His-tag (1:2000, Invitrogen) were followed by secondary HRP conjugated antibody (1:2000 ...
-
bioRxiv - Microbiology 2021Quote: ... Primary antibodies against the His-tag (1:2000, Invitrogen) were followed by secondary HRP conjugated antibodies (1:2000 ...
-
bioRxiv - Biophysics 2019Quote: ... 1 nM LanthaScreen Elite Tb-anti-His Antibody (ThermoFisher), and 400 TRAP220 peptide in a buffer containing 20 mM potassium phosphate (pH 7.4) ...
-
bioRxiv - Microbiology 2022Quote: ... anti-His×6 (mouse monoclonal, Invitrogen, 1:10,000-20,000), anti-OmpA (rabbit polyclonal ...
-
bioRxiv - Immunology 2024Quote: ... 1 mg of magnetic beads (Dynabeads His-Tag, Invitrogen) were coupled with 200 µg of histidine-tagged SARS-CoV-2 S protein ...
-
bioRxiv - Cancer Biology 2021Quote: ... 1% Dispase II (Gibco) and 30 μg/ml DNase I in PBS for 45 min at 37°C ...
-
bioRxiv - Neuroscience 2021Quote: ... collagen IV (anti-collagen IV, COL-94, ThermoFisher Scientific MA1-22148), NeuN (anti-NeuN ...
-
bioRxiv - Biochemistry 2023Quote: ... the elutes of GlnR147 (his-TEV GlnR C60SL147C) from Ni-NTA column were cleaved with recombinant tobacco-etch virus protease (Life Technologies) overnight at 4 °C ...
-
bioRxiv - Immunology 2019Quote: ... and human bronchial carcinoma Calu3 epithelial cells (ATCC_HTB-55) were grown in 10% FCS in alpha MEM media (Gibco). Human alveolar epithelial carcinoma A549 epithelial cells (ATCC_CCL-185 ...
-
bioRxiv - Cell Biology 2020Quote: ... Human alpha thrombin (Nordic Diagnostica AB, Billdal, Sweden) was resuspended in OptiMEM reduced serum medium (RSM) (Thermo Fisher Scientific) to 1 U/μl and stored at −20 °C ...
-
bioRxiv - Microbiology 2023Quote: Human pharyngeal carcinoma Detroit 562 epithelial cells (ATCC_CCL-138) were grown in 10% FCS in alpha MEM media (Gibco). For each set of experiments ...
-
bioRxiv - Immunology 2022Quote: ... The recombinant bacmid was extracted and further transfected into Sf9 cells using Cellfectin II (Invitrogen, #10362100). The recombinant viruses were harvested from the transfected supernatant and amplified to generate high-titer virus stock ...
-
bioRxiv - Evolutionary Biology 2022Quote: ... were transfected with recombinant bacmids using Cellfectin II reagent (Gibco-Thermo Fisher Scientific™; Cat#10362100). After a three-day incubation period ...
-
bioRxiv - Molecular Biology 2022Quote: ... The recombinant bacmid was transfected into Sf9 cells using Cellfectin® II reagent (Thermo Fisher Scientific). The Sf9 cells were incubated for 120 hours at 28 to produce P1 baculoviruses ...
-
bioRxiv - Immunology 2023Quote: ... cells at the end of 1-day culture were pulsed for 20 minutes with 40 ng/ml of recombinant human IFN-γ (Gibco/Thermo Fisher), 100 IU/ml of recombinant human IL-2 (Roche Diagnostics ...
-
bioRxiv - Cancer Biology 2023Quote: ... were cultured in in K-SFM + Human recombinant Epidermal Growth Factor (rEGF) and Bovine Pituitary Extract (BPE) supplemented with 1% penicillin and streptomycin (all from Thermo Fisher Scientific). PCa cell line PC-3 (CRL-1435) ...
-
bioRxiv - Bioengineering 2023Quote: ... type 1 (0.4 mg/mL, GIBCO™) for 1 h at 37 °C ...
-
bioRxiv - Bioengineering 2023Quote: ... type 1 (0.4 mg/mL, GIBCO™) for 1 h at 37 °C to degrade the collagen for flow cytometry experiments ...
-
bioRxiv - Immunology 2024Quote: ... 1 mg/ml collagenase type IV (Gibco), and 0.2 mg/ml DNase (Sigma-Aldrich) ...
-
bioRxiv - Synthetic Biology 2023Quote: Tetra-cysteine (TC) tagged proteins (CCPGCC) were site-specifically labelled using the TC-ReAsH II TC detection dye (Invitrogen). TC-HERD-2.2–TnaA and TC-HERD-2.2–FMO (100 μM ...
-
bioRxiv - Developmental Biology 2022Quote: ... The bottom layer of the culture was first prepared by pouring 100 μL of type I collagen mix solution into each well of an 8-well coverglass chamber (177402PK, Nunc, Roskilde, Denmark). The middle layer of the culture was prepared by adding 100 μL of the type I collagen mix solution containing 3 × 103 cells/well of the Klf5-iKO line and laid over the bottom layer ...
-
bioRxiv - Cell Biology 2021Quote: ... cells were plated in the center of the well by spotting 9000 cells on 3uL of type I collagen (Gibco A10483-01). Following treatment ...
-
bioRxiv - Cell Biology 2020Quote: ... LPS-stimulated BMDCs (2.5×105 cells per well) were seeded into 3D collagen (type I, rat tail)/Matrigel matrix (both from Corning, via Thermo Fisher Scientific) supplemented with 10% minimum essential medium alpha medium (MEMalpha ...
-
bioRxiv - Cell Biology 2021Quote: ... transfected cells were seeded at low density 1000 cells/well in 96-well microtiter IncuCyte® ImageLock Plate (Essen BioScience) pre-coated with rat tail Collagen type I (Gibco™). After 24Lhours ...
-
bioRxiv - Cell Biology 2021Quote: ... cells were seeded onto a 96-well microtiter IncuCyte® ImageLock Plate (Essen BioScience) pre-coated with rat tail Collagen type I (Gibco™). When cells were at full confluence ...
-
bioRxiv - Molecular Biology 2024Quote: ... transfected cells were seeded at the density of 2000 cells/well in 96-well microtiter IncuCyte® ImageLock Plate (Essen BioScience) pre-coated with rat tail Collagen type I (Gibco BRL) overnight ...
-
bioRxiv - Microbiology 2021Quote: ... The cells were then stimulated with 20 ng/mL of recombinant human IL-4 (Gibco) and 20 ng/mL of recombinant human IL-13 (Sigma-Aldrich ...
-
bioRxiv - Cancer Biology 2020Quote: ... One ng/mL recombinant human interleukin (IL)-6 (Gibco, Thermo Fisher Scientific, Waltham, MA, USA), was added to the media of the IL-6 dependent myeloma cell lines INA-6 ...
-
bioRxiv - Cancer Biology 2020Quote: ... 10ng/ml recombinant human basic Fibroblast Growth Factor (bFGF) and 1x B27 supplement (Life Technologies). Cells were then counted and passed through a 25G needle to obtain a single cell suspension ...
-
bioRxiv - Cell Biology 2022Quote: ... were combined with 0.36 μg of recombinant human protein CDK1/Cyclin B (Thermo Fisher, PV3292), 1x Kinase Buffer (Cell Signaling ...
-
bioRxiv - Immunology 2022Quote: ... 2-mercaptoethanol (RP-10) and 10 ng/ml recombinant human IL-2 (Thermo Fisher Scientific) for 18-22 hours ...
-
bioRxiv - Immunology 2020Quote: ... Recombinant human TNF-α (PHC3011) and antibody against OAS3 (PA5-31090) was from Thermo Fisher Scientific ...
-
bioRxiv - Genetics 2022Quote: ... Cells were resuspended in S12 media supplemented with 4 mg/L Recombinant Human Insulin (Gibco) (S12+ media) ...
-
bioRxiv - Bioengineering 2022Quote: ... the medium was supplemented with Human Interferon-gamma Recombinant Protein (IFN-γ) (ThermoFisher, Cat. #. PHC4031) at a concentration of 50 ng/mL ...
-
bioRxiv - Pathology 2022Quote: ... Cells were resuspended in S12 media supplemented with 4 mg/L Recombinant Human Insulin (Gibco) (S12+ media) ...
-
bioRxiv - Cell Biology 2023Quote: Reconstitute Human FGF-basic (FGF-2/bFGF) Recombinant Protein (here we use ThermoFisher 13256-029). Briefly ...
-
bioRxiv - Cell Biology 2023Quote: ... supplemented with human recombinant epidermal growth factor and bovine pituitary extract (Thermo Fisher Scientific AG), 1% penicillin and streptomycin ...
-
bioRxiv - Neuroscience 2024Quote: ... and plated in the fibroblast medium onto truncated recombinant human vitronectin (VTN-N, Gibco, A14700)-coated plates ...
-
bioRxiv - Neuroscience 2023Quote: ... Purified recombinant human antibodies (rHumAbs) were desalted (Zeba™ Spin Desalting Columns, Thermo Fisher 89891) and concentrated using a Christ Rotational Vacuum Concentrator System ...
-
bioRxiv - Immunology 2024Quote: ... and recombinant human interleukin 4 (rhIL-4) (300 U/mL; Thermo Fisher Scientific, Waltham, MA). On day 2 and day 5 post-monocyte isolation ...
-
bioRxiv - Cell Biology 2019Quote: Human embryonic stem cells (hESCs) and human induced pluripotent stem cells (hiPSCs) were cultured on Vitronectin (VTN-N) recombinant human protein (ThermoFisher Scientific, A14700). Culture vessels were coated with 200μL/cm2 of Vitronectin that had been diluted to 6μg/ml with PBS and incubated at 37°C for at least 1 hour ...
-
bioRxiv - Immunology 2022Quote: ... 1% MEM NEAA and 50 Units/mL recombinant human IL-2 (Myltenyi; #130-097-745) with Dynabeads™ Human T-activator CD3/CD28 (Gibco, #11132D) the same day of the purification (day 0 ...
-
bioRxiv - Immunology 2019Quote: ... total PBMC or IEL were cultured in the presence or absence of recombinant human osteopontin (2 μg/ml) (R&D) and anti-human-CD44 (5 μg/ml) (Thermofisher; clone IM7).
-
bioRxiv - Neuroscience 2019Quote: FLAG-tagged human LRRK2 proteins were enriched from injected rat striatum using Protein G-Dynabeads (50 µl; Invitrogen) pre-coupled with mouse anti-FLAG-M2 antibody (5 µg ...
-
bioRxiv - Molecular Biology 2023Quote: ... Human PKCβII was YFP-tagged at the N-terminus in a pcDNA3 vector using Gateway Cloning (Life Technologies) as described in (6) ...
-
bioRxiv - Immunology 2023Quote: ... recombinant human IL-3 (R&D, Cat. 203-IL-050/CF, 25 ng mL-1) or c) human M-CSF recombinant protein (Invitrogen, Cat. PHC9501, 100 ng mL-1). A partial medium change was performed every 48 hours with 2x cytokine composites to replenish cytokines ...