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Citations for Thermo Fisher :
551 - 600 of 10000+ citations for Recombinant Human Collagen Type II Alpha 1 His tagged since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2021Quote: ... reaction mixes were prepared using 2.5 μg recombinant His-ACBD5 with or without the addition of 0.3 mM ice-cold ATP (Thermo Fisher) and 0.1 ug GST-GSK3β (Abcam) ...
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bioRxiv - Cancer Biology 2023Quote: ... including anti-collagen I (1:200, Thermo Fisher Scientific), anti-collagen IV (1:200 ...
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bioRxiv - Microbiology 2021Quote: ... 5% A+ human serum and 5% AlbuMAX II (Life Technologies). Gametocyte media was changed daily without the addition of fresh erythrocytes for 14 days following induction ...
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bioRxiv - Genetics 2022Quote: 3T3-L1 cells expressing GFP-tagged human CIDEC were incubated with MitoTracker (#M7512; Thermo Fisher Scientific) before fixation ...
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bioRxiv - Neuroscience 2020Quote: ... recombinant human epidermal growth factor (5 ng/ml) and additionally supplemented with freshly added recombinant human fibroblast growth factor-basic (1 ng/ml; Gibco). hCMEC/D3 cells used for the experiments were between passages 6 and 11 ...
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bioRxiv - Molecular Biology 2021Quote: ... or Alexa647-tagged antisera (1:500; Invitrogen, Karlsruhe, Germany) in TBS ...
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bioRxiv - Microbiology 2020Quote: ... NR-52285) using the primers in Table II and cloned into pCDNA-V5/His TOPO (Invitrogen). We introduced a FLAG tag to JUNV GP by PCR amplifying it from a previously described expression plasmid (58 ...
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bioRxiv - Biophysics 2022Quote: The gene encoding N-terminal His-tagged V2HeR3 was cloned into the EcoRI and XbaI site of pPICZB vector (Thermo Fisher Scientific). The recombinant protein was expressed in the Pichia pastoris strain SMD1168H (Philosof et al. ...
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bioRxiv - Biophysics 2022Quote: ... the gene encoding N-terminal His-tagged V2HeR3 was cloned into the EcoRI and XbaI site of pPICZB vector (Thermo Fisher Scientific). For determination of λmax of V2HeR3 WT and mutants ...
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bioRxiv - Microbiology 2021Quote: ... Purification of His-tagged Cg1978 was performed by applying the supernatant to an equilibrated 2 ml Ni-NTA column (Invitrogen, Kalifornien, USA). After washing with 30 ml TNI20 buffer ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: His-tagged relaxins were expressed as secreted proteins in Expi293F cells containing a stably integrated tetracycline repressor (Expi293F tetR, Thermo Fisher Scientific) grown in Expi293 media (Thermo Fisher Scientific) ...
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bioRxiv - Immunology 2022Quote: ... Expi293F™ cells were transiently transfected with His-tagged gB ectodomain plasmids for five days and later harvested with Nickel-NTA resin (Thermo Fisher Scientific).
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bioRxiv - Cell Biology 2021Quote: ... 15 mM HEPES DMEM/F12 no phenol red) were seeded onto plates coated with 50 μg/ml collagen type I from rat tail (ThermoFisher) for 1 to 2h at room temperature ...
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bioRxiv - Developmental Biology 2020Quote: ... Each slice was embedded into 10 μl of rat type I collagen (Roche Diagnosis; diluted at 80% with 1X MEM (GIBCO), 1X GlutaMax (GIBCO ...
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bioRxiv - Developmental Biology 2021Quote: ... the polyacrylamide sheet was washed twice and incubated with a solution of type I collagen (0.2 mg/ml; Gibco BRL) overnight at 4°C.
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bioRxiv - Cell Biology 2022Quote: ... Fresh primary mouse hepatocytes were cultured on Type I collagen-coated 60 mm Permanox plates (Thermo Scientific, Grand Island, NY) in William’s E Medium (Gibco ...
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bioRxiv - Neuroscience 2023Quote: ... explants were seeded in 96-well plates coated with poly-L-lysine/collagen type I and neurobasal medium supplemented with of 1X B27 (50X Gibco), 20 μM L-glutamine ...
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bioRxiv - Cancer Biology 2023Quote: ... Cells were seeded at the final density of 2×106 cells/ml in the central chamber of the DAX-1 chips embedded in a matrix composed of type I rat tail collagen at the final concentration of 2.3 mg/ml (Thermofisher, #A1048301). Each chip can be loaded with 10µl of gel ...
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bioRxiv - Cancer Biology 2022Quote: ... This suspension was in turn dissolved in a mixture of 50% Collagen type I (4mg/ml; Thermo Fisher Scien-tific), 25% Matrigel ...
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bioRxiv - Neuroscience 2023Quote: ... Full-length human LRRC4B was subcloned and inserted into the pcDNA6-V5-His vector (Invitrogen). FAM19A5 (NM_001252310.1 ...
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bioRxiv - Cell Biology 2021Quote: ... Cells were transiently transfected with mCherry-tagged wild type or mutant ORP1L at 70% confluency using Lipofectamine 2000 reagent (Invitrogen) according to the manufacturer’s protocol ...
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bioRxiv - Cancer Biology 2022Quote: ... Minced tissues were incubated in digestion medium (Collagenase type II 5 mg/ml [GIBCO] ...
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bioRxiv - Bioengineering 2019Quote: Droplets were created with deionized (DI) water (Type II, Harleco; Fisher Scientific, Hampton, NH) tinted with either yellow or green dye (Spice Supreme ...
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bioRxiv - Cell Biology 2023Quote: ... The following morning the cardiomyocytes undergo digestions with collagenase type II (ThermoFisher Scientific, 17101015) in HBSS ...
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bioRxiv - Bioengineering 2023Quote: ... The subchondral bone samples were treated with collagenase Type II (Thermo Fisher Scientific, Australia) before washing with 1× PBS 3 to 4 times to remove the debris ...
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bioRxiv - Cancer Biology 2023Quote: ... Minced tissues were incubated in digestion medium (Collagenase type II 5 mg/mL [GIBCO] ...
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bioRxiv - Microbiology 2023Quote: ... and the Type II MTases M.HpyAI (HP1208) and M.HpyAII (HP1368) were synthesized by ThermoFisher. The sequences of all probes and reporters used can be found in Supplementary Table 2 ...
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bioRxiv - Immunology 2022Quote: ... 10% hi-FBS and 1% penicillin/streptomycin (ThermoFisher) in absence or presence of APRIL (Recombinant Human APRIL/TNFSF13 Protein ...
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bioRxiv - Cell Biology 2023Quote: ... mouse anti-His (Invitrogen, #MA1-21315, 1:1000), rabbit anti-GST (Merck,#ZRB1223,1:1000) ...
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bioRxiv - Genetics 2021Quote: Wild-type human FAN1 gene was cloned in a derivative of pFASTBAC1 (Invitrogen) with GST- ...
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bioRxiv - Cell Biology 2023Quote: ... containing 1 mg.ml-1 collagenase type IV (Gibco) and 2 mg.ml-1 dispase (Gibco) ...
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bioRxiv - Immunology 2022Quote: Expression vectors for human His-NLRP1PYD (aa 1-92) and GST-NLRP1PYD were generated by Gateway cloning with destination vector pDEST17 (Thermo Fisher Scientific) and a customized destination vector based on pGEX-2T (a kind gift of Mikko Taipale ...
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bioRxiv - Immunology 2021Quote: ... and RBD-his tag protein-bound antibody was detected with goat-anti-human IgG1 (H+L) with horseradish peroxidase (HRP) conjugated (Invitrogen,1:1000) The plates were washed and developed using TMB substrate solution (Biolegend ...
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bioRxiv - Cell Biology 2022Quote: ... differentiated ALI-cultures were apically washed with PBS and treated with collagenase type II (1 mg/mL; Gibco, Waltham, MA, USA), diluted in advanced DMEM/F12 ...
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bioRxiv - Physiology 2024Quote: ... The blocks were then treated with 1 mg/ml collagenase type II in Leibovitz’s L-15 Medium (Thermo Fisher Scientific Inc.) at 37°C for 30-45 minutes ...
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bioRxiv - Cancer Biology 2019Quote: ... The fragments were digested in digestion medium (advanced DMEM/F12 with 2% FBS, Pen/Strep, 100 U/mL collagenase type XI [SigmaAldrich], and 125 μg/mL dispase type II [Invitrogen, Waltham, MA, USA]) at 37 °C for 40 min and then further digested for 10 min by adding a half-volume of TrypLE Express ...
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bioRxiv - Microbiology 2020Quote: One 96-well plate was coated with 200ng/well of recombinant N protein and another plate was coated with 200ng/well of recombinant 6xHis-tagged SARS-CoV-2 spike protein receptor binding domain (RBD) produced using the FreeStyle 293 Expression System (Thermo Fisher) from BEI Resources (Cat# NR-52366 ...
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bioRxiv - Cell Biology 2021Quote: ... The His-tagged recombinant fusion protein was separated by cobalt immobilized metal chelate affinity beads using the HisPur Cobalt Purification Kit (Thermo Scientific) following the procedure described by the manufacturer ...
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bioRxiv - Cell Biology 2020Quote: Recombinant 6XHis-tagged TbVps24 (N-terminus tag) was cloned using the pTrcHis TOPO TA expression system (Thermo Fisher Scientific, Carlsbad, CA) as per the manufacturer’s instructions ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: Human MSCs at passage 2 were maintained in growth media consisted of alpha-MEM (Life Technologies, 32561--037) supplemented with 17% FBS (Sigma-Aldrich ...
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bioRxiv - Microbiology 2022Quote: ... A recombinant SPATR (rSPATR) fused to a polyhistidine tag (His-tag) was produced in BL21-CodonPlus-RIL competent cells (Invitrogen) and protein purification was performed under denaturing conditions using Ni-NTA Agarose (Qiagen ...
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bioRxiv - Biochemistry 2023Quote: Plasmids for recombinant protein expression were constructed using mega primer insertion PCR [64] using the plasmid Champion pET303/CT-His vector (Invitrogen). The double-stranded DNA inserts (Integrated DNA Technologies ...
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bioRxiv - Microbiology 2020Quote: ... in MEM-alpha (Gibco) with 20% FBS (Hyclone) ...
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bioRxiv - Bioengineering 2023Quote: ... in alpha-MEM (Gibco). MSCs were cultured to 80% confluence following standard protocols (65) ...
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bioRxiv - Neuroscience 2021Quote: ... supplemented with 20 ng/mL human recombinant basic fibroblast growth factor (bFGF; Invitrogen, USA), 20 ng/mL epidermal growth factor (EGF ...
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bioRxiv - Genetics 2019Quote: ... cells were incubated in differentiation medium (DM) (10 µg/ml recombinant human insulin (Gibco), 2% HI horse serum (Thermo Fisher) ...
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bioRxiv - Cell Biology 2022Quote: ... Basal Medium plus 50 ng/ml human recombinant VEGFA (PHC9391 Gibco, Frederick, MD, USA) or Basal Medium plus 100 ng/ml recombinant human NTN1 (6419-N1 ...
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bioRxiv - Developmental Biology 2019Quote: ... Cells were cultured on 6-well plates coated with Vitronectin Recombinant Human Protein (Invitrogen) at a concentration of 0.5 µg/cm2 ...
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bioRxiv - Cell Biology 2022Quote: ... 4h prior treatment with recombinant human epidermal growth factor (EGF) (Cat# RP-10927, Invitrogen) at 5 ...
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bioRxiv - Immunology 2023Quote: ... supplemented with 294.4 ng/l human recombinant Epidermal Growth Factor (EGF) (Gibco, 10450-013), 25 mg/l Bovine Pituitary Extract (Gibco ...