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Citations for Thermo Fisher :
901 - 950 of 10000+ citations for Recombinant Human Collagen Type II Alpha 1 His tagged since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2021Quote: ... The expression vectors encoding mHVEM (Q39-Q206) fused with human IgG1 and a subsequent hexa-His tag sequences were transfected into Expi293 (Gibco) cells using ExpiFectamine 293 transfection kit (Gibco ...
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bioRxiv - Immunology 2021Quote: ... The DNA sequences encoding a protein biologic composed of mHVEM residues (Q39- Q206) followed by human IgG1 and a subsequent hexa-His tag sequences were cloned into pcDNA 3.3 vector (Life technologies) using In-fusion HD cloning enzyme premix (Clontech) ...
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bioRxiv - Immunology 2021Quote: ... were coated at 1 μg/mL 6x-His tag polyclonal antibody (Invitrogen) for >4 hours at ambient temperature and blocked ≥1 hour with 5% milk/1x PBS/0.01% Tween-20 at 4°C ...
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bioRxiv - Genetics 2022Quote: ... supplemented with 2% HI-FBS and 1% penicillin-streptomycin (Gibco 15140-122). Cells were maintained in humidified incubators at 37°C and 5% CO2 ...
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bioRxiv - Immunology 2023Quote: ... and coated with 1 mg of magnetic beads (Dynabeads His-Tag, Invitrogen) as previously described21 ...
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bioRxiv - Evolutionary Biology 2020Quote: ... 10 ml bMSC media (MEM-alpha [ThermoFisher, Waltham ...
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bioRxiv - Bioengineering 2019Quote: ... and alpha Minimum Essential Medium (αMEM, Gibco) then left overnight at room temperature ...
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bioRxiv - Bioengineering 2020Quote: ... alpha smooth muscle actin (Invitrogen PA5-18292) or cTnT (Abcam ab8295 ...
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bioRxiv - Cancer Biology 2020Quote: ... Alpha-tubulin (MA1-80189, Thermo Fisher Scientific), Ubiquitin (BML-PW8810 ...
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bioRxiv - Bioengineering 2020Quote: ... mouse anti-alpha-fetoprotein (MA1-19342; Invitrogen) diluted 1:500 and DAPI (D1306 ...
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bioRxiv - Cell Biology 2021Quote: ... alpha modification (α-MEM; Invitrogen, Carlsbad, CA) supplemented with 10% fetal bovine serum (FBS ...
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bioRxiv - Immunology 2022Quote: ... IFN-alpha rabbit polyclonal (#PA5-115430 ThermoFisher). CD123-goat polyclonal (#ab205365 Abcam ...
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ISL2 is an epigenetically silenced tumor suppressor and regulator of metabolism in pancreatic cancerbioRxiv - Molecular Biology 2020Quote: ... and alpha-tubulin (Thermo Fisher Scientific # 62204) were used in the western blot.
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bioRxiv - Cell Biology 2021Quote: ... with MSC derivation medium (alpha-MEM (Invitrogen), 10% FBS (Hyclone) ...
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bioRxiv - Cell Biology 2020Quote: ... Alpha-tubulin (Thermo-Fisher Scientific, MA1-80189), Active-β-catenin (anti-ABC ...
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bioRxiv - Genomics 2021Quote: ... Differentiation Media: MEM alpha (Gibco 12571-063) supplemented with 10% FBS ...
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bioRxiv - Bioengineering 2022Quote: ... in Minimum Essential Medium (MEM) Alpha (Gibco™ MEM α ...
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bioRxiv - Synthetic Biology 2022Quote: ... coli strain DH5-alpha (Invitrogen, 18265-017) was used for molecular cloning ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: Alpha-asarone powder was obtained from ThermoFisher Gmbh (Kandel ...
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bioRxiv - Neuroscience 2024Quote: ... mouse anti-CAMKII alpha (ThermoFisher, MA1-048), rat anti-GAPDH (Biolegend ...
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bioRxiv - Cell Biology 2024Quote: ... rat anti-alpha tubulin (MA1-80017, Invitrogen), and human anti-centromere protein antibody (Antibodies Incorporated) ...
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bioRxiv - Cancer Biology 2022Quote: ... Minced tissues were incubated in digestion medium (Collagenase type II 5 mg/ml [GIBCO], Dispase 5 mg/ml [GIBCO] ...
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bioRxiv - Bioengineering 2022Quote: ... the recellularized liver after perfusion culture was incubated with RPMI (Wako) containing 10 mg/mL collagenase type II (Gibco) for 15 min at 37°C ...
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bioRxiv - Bioengineering 2022Quote: ... after which an additional 440 U of Type 1A collagenase was added along with Dispase II (Life Technologies, #17105041) and DNAse I (Bio Basic ...
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bioRxiv - Genomics 2022Quote: ... Cardiac tissue pieces were then digested in tissue dissociation media with 200U/mL collagenase type II (Gibco 17100-015), 1 mg/ml dispase (Sigma D4693) ...
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bioRxiv - Bioengineering 2023Quote: ... Tissues were minced and then digested with 100U/ml of Collagenase Type II (Gibco, Life Technologies, Carlsbad, CA, USA) in Phosphate Buffered Saline (PBS ...
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bioRxiv - Bioengineering 2023Quote: ... Tissues were minced and then digested with 100U/ml of Collagenase Type II (Gibco, Life Technologies, Carlsbad, CA, USA) in Phosphate Buffered Saline (PBS ...
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bioRxiv - Cancer Biology 2023Quote: ... Minced tissues were incubated in digestion medium (Collagenase type II 5 mg/mL [GIBCO], Dispase 5 mg/mL [GIBCO] ...
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bioRxiv - Bioengineering 2022Quote: ... Primary human MSCs (Cyagen Biosciences, Suzhou, China) from bone marrow were cultured in a minimum essential medium alpha (MEM-α, Gibco, Carlsbad, CA) medium with 10% fetal bovine serum (FBS ...
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bioRxiv - Microbiology 2020Quote: ... Tsp-his was detected with the Anti-6X His tag antibody (ThermoFisher Scientific), which is already conjugated with HRP and does not require a secondary antibody for detection ...
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bioRxiv - Developmental Biology 2023Quote: ... For antibodies we used a His antibody (6x-His Tag Monoclonal Antibody, ThermoFisher), a MBP antibody (Anti-MBP Monoclonal Antibody ...
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bioRxiv - Molecular Biology 2024Quote: T7 double stranded DNAs (T7-dsDNAs) were amplified by T7-tagged primer pairs (Table 2) by Phire Hot Start II PCR Master Mix kit (Thermo Fisher, Cat # F125L) using proper plasmids as templates ...
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bioRxiv - Immunology 2019Quote: ... pneumoniae for 6 hours in 1% FCS phenol free alpha MEM (base media, Life Technologies). Cells were washed three times in PBS and gently lifted from the plate using a cell scraper in 300μl of base media supplemented with 1mM EDTA ...
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bioRxiv - Cancer Biology 2021Quote: MM-1 feeder cells were cultured in MEM Alpha+GlutaMAX medium (ThermoFisher Scientific, Winsford, UK) supplemented with 10% FBS (Gibco) ...
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bioRxiv - Physiology 2020Quote: ... Smooth Muscle Actin Alpha (SMA or ACTA2, Invitrogen, PA5-18292, RRID: AB 10980764, 1:100), Anti-Goat 488 (Jackson ImmunoResearch ...
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bioRxiv - Cancer Biology 2021Quote: ... Antibodies used were as follows: mouse anti alpha-SMA (1:200, Invitrogen, Carlsbad, CA, USA); Myosin-IIB (1:1000 ...
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bioRxiv - Cell Biology 2020Quote: ... Anti-alpha-tubulin was detected using Alexa Fluor 488-goat anti mouse (Invitrogen, 1:1000). In addition ...
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bioRxiv - Cancer Biology 2022Quote: ... and mouse anti-alpha smooth muscle actin (Thermo Fisher Scientific; 14-9760-82; 1:400). The secondary antibodies were horseradish peroxidase- conjugated goat anti-rabbit IgG antibodies (Nichirei ...
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bioRxiv - Immunology 2023Quote: ... and TNF (PE rat anti-mouse TNF alpha, 1:100; Invitrogen, Cat. 12-7321-82) for 30 min at RT ...
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bioRxiv - Microbiology 2021Quote: ... The bacmid was transfected into Sf9 cells to obtain recombinant baculovirus using Cellfectin II Reagent (Invitrogen Co., Carlsbad, CA, USA) according to the manufacturer’s instructions.
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bioRxiv - Microbiology 2019Quote: ... Eight μL of Cellfectin II reagent and 3 ng of each recombinant plasmid were diluted in 100 μL Grace’s medium (Thermofisher Scientific), respectively ...
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bioRxiv - Microbiology 2019Quote: ... Stable cell lines expressing the recombinant protein were initially maintained in complete medium comprising of serum free medium Sf-900 II SFM (ThermoFisher) complemented with 10% v/v foetal bovine serum (Sigma) ...
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bioRxiv - Developmental Biology 2023Quote: ... transgenic fragments were further shuffled into the pLenti-Gateway vector via Gateway LR recombinant reaction using Gateway LR Clonase II Enzyme Mix (Invitrogen). In the case of KCNA2 and OVOL1 ...
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bioRxiv - Genetics 2021Quote: ... collagen-coated well (Gibco, A11428-02) and passaged 1:3 while subconfluent until reaching 85% confluence on a 10cm diameter dish ...
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bioRxiv - Bioengineering 2020Quote: ... collagen I rat protein (Gibco, A1048301), x10 phosphate-buffered saline (PBS ...
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bioRxiv - Cancer Biology 2019Quote: ... Collagen I was from Thermo Fisher Scientific ...
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bioRxiv - Physiology 2021Quote: ... rat tail collagen I (Invitrogen, USA) at the final concentration of 6 or 10 µg/cm2 ...
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bioRxiv - Cell Biology 2022Quote: ... coated with bovine collagen I (Gibco) was used in RHPE ...
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bioRxiv - Cell Biology 2019Quote: ... collagen I (Col I) (Fisher Scientific) overnight at 4°C ...
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bioRxiv - Systems Biology 2020Quote: ... Collagen (Thermo Fisher Scientific, Catalogue #A1048301), sterile 10X phosphate buffered saline (Thermo Fisher Scientific ...