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Citations for Thermo Fisher :
801 - 850 of 10000+ citations for Recombinant Human Collagen Type II Alpha 1 His tagged since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2020Quote: ... using the primers described in Table II followed by restriction digestion with BamHI and XbaI and ligation into pcDNA-V5/His (Invitrogen). The MV H and HPIV-1 HN were PCR amplified using the primers shown in Table II from genomic RNA of MV ...
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bioRxiv - Developmental Biology 2021Quote: ... were coated with 10 μg/ml recombinant human laminin 521 (BioLamina) diluted in DPBS (Thermo Fisher Scientific) for 3 hours at 37°C and washed with DPBS twice ...
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bioRxiv - Neuroscience 2019Quote: ... and 5 μg/mL human recombinant laminin 521 (BioLamina #LN521-02) in E8 basal medium (Gibco #A1517001) supplemented with 1% Penicillin/Streptomycin (Pen/Strep ...
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bioRxiv - Microbiology 2022Quote: Recombinant human CD81 protein (R &D Systems) was coated (4µg/ml) on ELISA plate (Thermo Fisher Scientific) and incubated overnight at 40C ...
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bioRxiv - Neuroscience 2020Quote: ... and 5 μg/mL human recombinant laminin 521 (BioLamina #LN521-02) in E8 basal medium (Gibco #A1517001) supplemented with 1% Penicillin/Streptomycin (Pen/Strep ...
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bioRxiv - Immunology 2020Quote: ... M0 cells were treated for two days with 20 ng/ml recombinant human IFN-y (Life Technologies) and 50 ng/ml Escherichia coli LPS (SigmaAldrich ...
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bioRxiv - Cancer Biology 2022Quote: ... The medium was supplemented with 15% fetal bovine serum (FBS) and recombinant human insulin (Gibco 12585-014) for pancreatic cancer cells and with 10% FBS for both human lung and colon cancer cells ...
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bioRxiv - Neuroscience 2024Quote: ... hiPS cells were cultured on six-well plates coated with recombinant human vitronectin (VTN–N, Gibco, A14700) in Essential 8 medium (Gibco ...
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bioRxiv - Microbiology 2021Quote: ... 5x-His mouse anti-tag was used at 1:500 (Invitrogen). Anti-mouse HRP secondary antibody was used at 1:10,000 (Invitrogen) ...
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bioRxiv - Biophysics 2019Quote: ... 1 nM LanthaScreen Elite Tb-anti-HIS Antibody (Thermo Fisher Scientific), and 5 nM Fluormone Pan-PPAR Green (Invitrogen ...
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bioRxiv - Immunology 2021Quote: ... washed with 1% HI-FBS RPMI-1640 (ThermoFisher Scientific cat. 11875085) and red blood cells were lysed using 1x RBC Lysis Buffer (eBioscience ...
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bioRxiv - Immunology 2021Quote: ... 1◻nM LanthaScreen Elite Tb-anti-His Antibody (ThermoFisher #PV5895), and 400 nM FITC-labeled PGC1α peptide (residues 137–155 ...
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bioRxiv - Microbiology 2023Quote: ... 1-2 μl of primary anti-His (PA1-983B, Thermo Scientific) or anti-alpha RNAP (4RA2 ...
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bioRxiv - Molecular Biology 2022Quote: ... 1 µL of His-Tag magnetic beads (Dynabeads™ Invitrogen #10103D) was added to each tube ...
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bioRxiv - Microbiology 2021Quote: ... sodium bicarbonate and either a) 10% heat-inactivated A+ human plasma (Interstate Blood Bank, Memphis, TN) or b) 1% (w/v) AlbuMAX II (Thermo Fisher) and 320 μM hypoxanthine (Sigma) ...
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bioRxiv - Microbiology 2021Quote: SARS-CoV-2 (438-516) S-RBD((HiS)6 and biotynilated human ACE2 have been purchased from Fisher Scientific (respective references 16534204 and 16545164) ...
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bioRxiv - Biophysics 2019Quote: ... FLAG-tagged hZP3 and HA-tagged hZP4 were synthesized (GenScript; GeneArt/Thermo Fisher Scientific) and subcloned into pHLsec329 ...
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bioRxiv - Bioengineering 2022Quote: ... collagen was acquired from Gibco. Phosphate buffer saline (PBS ...
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bioRxiv - Bioengineering 2021Quote: ... collagen were purchased from Gibco and Phosphate buffer saline (PBS ...
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bioRxiv - Cancer Biology 2022Quote: ... precoated with collagen I (Gibco), ~5-10 x 103 cells in 200 μL DMEM per well ...
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bioRxiv - Cancer Biology 2021Quote: ... and collagen I (Life Technologies) and seeded onto 35 mm glass bottom culture dishes (MatTek) ...
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bioRxiv - Cancer Biology 2021Quote: ... and collagen I (Life Technologies) and seeded onto 35 mm glass bottom culture dishes (MatTek) ...
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bioRxiv - Cell Biology 2022Quote: ... coated with collagen I (Gibco) and cultured for 24 hrs before imaging ...
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bioRxiv - Cancer Biology 2024Quote: ... Collagen IV (Invitrogen #PA5-104508), pDDR1 (Tyr513 ...
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bioRxiv - Bioengineering 2022Quote: ... and digested for 16 h at 37 °C in 1.5 mg/mL collagenase type II solution (Gibco, Canada) in basal media ...
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bioRxiv - Cell Biology 2021Quote: ... 0.25% trypsin/ethylene diamine tetraacetic acid(EDTA) and type II collagenase were purchased from Gibco(Gaithersburg, MD, USA). Trizol and Lipofectamine 3000 was purchased from Invitrogen (Carlsbad ...
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bioRxiv - Developmental Biology 2022Quote: ... The sliced tissue was then transferred to a glass tube along with a magnetic stir bar and 1.5 ml digestion buffer in Dulbecco’s modified Eagle’s medium containing collagenase type II (250 U/ml) (17101015; Gibco), collagenase type IV (300 U/ml ...
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bioRxiv - Molecular Biology 2022Quote: ... After digestion with Type II collagenase (2mg/mL in PBS/2% BSA, Life Technologies Inc., Carlsbad, CA, USA) at 37°C for 60 min ...
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bioRxiv - Synthetic Biology 2021Quote: ... Two types of FRT-KanR-FRT fragments were amplified using Platinum Polymerase II or Phusion polymerase (Thermo Fisher) with different flanking homology arms (41-43 bp ...
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bioRxiv - Bioengineering 2023Quote: ... cloning was achieved via one-pot restriction-ligation reactions with type IIS restriction enzymes (NEB or Thermo Scientific) and T4 DNA ligase (NEB) ...
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bioRxiv - Neuroscience 2022Quote: ... mouse alpha-smooth muscle actin –α-SMA antibody (Invitrogen, # 14-9760-82; 1:200), mouse TGF-β antibody (Abcam ...
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bioRxiv - Neuroscience 2022Quote: ... mouse alpha-smooth muscle actin –α-SMA antibody (Invitrogen, # 14-9760-82; 1:500), rabbit TGF-β antibody (Cell Signaling Technology ...
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bioRxiv - Molecular Biology 2020Quote: ... and alpha smooth muscle actin with SMaA-AF488 (1:100, 53-9760-82, Invitrogen). Aortic root plaques were also stained for CD68 (1:250 ...
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bioRxiv - Neuroscience 2020Quote: ... mouse monoclonal anti-CaMKII alpha subunit (clone 6G9, Thermo Scientific, #MA1-048, 1:1000), mouse monoclonal anti-NaV1.8 (clone N134/12 Neuromab Facility ...
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bioRxiv - Cell Biology 2024Quote: ... Complete MSC medium were prepared with MEM Alpha (1X) + GlutaMAX™ -1 (GIBCO, 32561037), PLTMax GMP Clinical Grade Supplement (Mill Creek Life Sciences ...
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bioRxiv - Bioengineering 2022Quote: ... Human bone marrow mesenchymal stem cells (hMSCs, RoosterBio, Maryland, USA) were cultured in alpha Minimal Eagle’s medium (α-MEM) (Gibco, Carlsbad, USA) with 10% FBS and 1% penicillin/streptomycin ...
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bioRxiv - Bioengineering 2024Quote: ... Human bone marrow mesenchymal stem cells (hMSCs, RoosterBio, Maryland, USA) were cultured in alpha Minimal Eagle’s medium (α-MEM) (Gibco, Carlsbad, USA) with 10% FBS (cat# 16141079 ...
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bioRxiv - Developmental Biology 2019Quote: ... Immunohistochemical analyses were performed on paraffin sections with traditional antigen retrieval and colorimetric development methodologies with the following primary antibodies: anti-Collagen II (Thermo Scientific, MS235B), anti-Collagen X (Quartett ...
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bioRxiv - Cell Biology 2022Quote: ... 1 mg/mL Collagenase Type I (Gibco 17100-017), and 0.1 mg/mL of DNase I (Roche 10104159001) ...
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bioRxiv - Developmental Biology 2021Quote: ... and 1 mg/ml type IV collagenase (17104019, GIBCO) for 30min at 37 ℃ with intermittent shaking ...
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bioRxiv - Cancer Biology 2023Quote: ... 1 mg/mL Collagenase Type I (Gibco 17100-017), and 0.1 mg/mL of DNase I (Roche 10104159001) ...
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bioRxiv - Molecular Biology 2022Quote: The human wild-type full-length D1R gene was cloned into a pcDNA3.1(+) vector (Thermo Fisher Scientific) with the signal peptide substituted by that of hemagglutinin (HA) ...
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bioRxiv - Cancer Biology 2020Quote: ... Normal cell type references were human neural stem cells induced from H9 ES cells (Invitrogen, N7800-100), Human iPSC-derived astrocytes (Tempo Bioscience) ...
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bioRxiv - Cancer Biology 2019Quote: ... 1 % (w/v) Dispase II (Gibco) and 30 μg/ml DNase I in PBS for 30-45 min at 37 °C ...
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bioRxiv - Cancer Biology 2019Quote: ... 1 mg/ml dispase II (Invitrogen) was added to the medium of the organoids and these were incubated for 15 min at 37° C to digest the BME ...
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bioRxiv - Cancer Biology 2019Quote: ... 1 mg/ml dispase II (Invitrogen) was added to the medium of the organoids and these were incubated for 15 min at 37° C to digest the BME ...
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bioRxiv - Immunology 2022Quote: ... 1 mg/ml Collagenase II (Thermofisher) and 1 mg/ml Pronase K (Roche ...
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bioRxiv - Cell Biology 2021Quote: ... SDHA (complex II) (1/1,000; Invitrogen), PRDM16 (1/1,000 ...
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bioRxiv - Systems Biology 2021Quote: ... Tn5 underwent a His-tag (Dynabeads His-Tag Isolation & Pulldown, Invitrogen, 10103D) clean-up according to the manufacturer’s protocol to remove unbound oligos before using.
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bioRxiv - Developmental Biology 2024Quote: ... Human TS cells were cultured in six-well plates precoated with 5 μg/mL of CorningTM collagen IV (CB40233, Thermo Fisher) and Basal Human TS Cell Medium [Dulbecco’s Modified Eagle Medium/F12 (DMEM/F12 ...