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Citations for Thermo Fisher :
651 - 700 of 10000+ citations for 5 Nitro 1H benzo de isoquinoline 1 3 2H dione since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2024Quote: ... 3% H2O2 (Fisher Scientific 7722-84-1) was used ...
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bioRxiv - Molecular Biology 2023Quote: ... 1-3 L of Expi293F cells (ThermoFisher) were grown at 37°C to a cell density of 3.2×106 cells/mL in FreeStyle expression medium (ThermoFisher) ...
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bioRxiv - Immunology 2022Quote: ... ICOS-SB436 (ISA-3, Invitrogen, 1:50), IgG-BV480 (goat polyclonal ...
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bioRxiv - Cell Biology 2024Quote: ... Lipofectamine RNAiMAX (Thermo Scientific, 13778030, 1:3), FuGene HD (Promega ...
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bioRxiv - Immunology 2021Quote: ... or control PBS added onto a slant of 2 mL of TSA within a 5 mL round bottom polypropylene tube (Thermo Fisher Scientific Inc., Wilmington, DE, USA) (Supplemental Figure S1) ...
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bioRxiv - Genetics 2021Quote: ... and subsequently counterstained with DAPI (1:30,000 of 5 mg/ml) for 3 minutes and mounted using Antifade reagent (ProLong Gold Antifade Reagent, Invitrogen, cat. number P36934). Images were taken and processed using an Airyscan980 SR confocal microscope (Zeiss).
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Turanose induced WOX5 restores symbiosis in the Medicago truncatula cytokinin perception mutant cre1bioRxiv - Plant Biology 2020Quote: ... and immersed and incubated in the dark in staining solution 1 mM 5-bromo-4-chloro-3-indolyl-β-D-glucuronicacid (X-Gluc, Thermo Scientific), 50mM sodium phosphate buffer ...
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bioRxiv - Cell Biology 2020Quote: ... Flow settings were adjusted to allow individual cells to become encapsulated with single hydrogel beads presenting cell-specific barcoding oligonucleotide primers inside 3-5 nL encapsulation mixture droplets that contain 20 U μL−1 reverse transcriptase (SuperScript III, Thermo Fisher Scientific), First-Strand Buffer (Thermo Fisher Scientific) ...
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bioRxiv - Biochemistry 2021Quote: ... One microgram of peptides in a volume of 1-4 µL was loaded onto the Acclaim µ-Precolumn (0.5 mm х 3 mm, 5 µm particle size, Thermo Scientific) at a flow rate of 10 µL/min for 4 min in an isocratic mode of Mobile Phase C (2% acetonitrile (Sigma-Aldrich) ...
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bioRxiv - Microbiology 2020Quote: ... and a newly designed 5’ FAM-labelled and 3’-minor groove binder (MGB)-modified probe SVIP-MP_P2-MGB ((Table 1; ThermoFisher Scientific, Eurofins Genomics). The primers encompass a 182 nucleotides-long amplicon ...
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bioRxiv - Cancer Biology 2020Quote: ... KTB21 cells were cultured in epithelial cell growth medium (DMEM (low glucose):Ham’s F12 (1:3) medium supplemented with 5% FBS (Thermo Fisher Scientific, USA), 0.4μL/mL hydrocortisone (Sigma ...
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bioRxiv - Microbiology 2021Quote: ... cDNA was synthesized using 0.2 units Klenow 3′→5′exo DNA polymerase (Enzynomics, Daejeon, ROK) and 1 μL RNaseH (Invitrogen, San Diego, CA). The Klenow reaction mixture was incubated at 37°C for 1 hr and 75°C for 15 min ...
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bioRxiv - Immunology 2021Quote: ... Sections were washed 3 x 5 minutes in PBS and stained with secondary antibodies donkey anti-rabbit Alexa Fluor 555 (1/1000, Invitrogen, A-31572) and donkey anti-goat Alexa Fluor 488 (1/1000 ...
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bioRxiv - Genetics 2023Quote: ... Ovaries were washed 3 times for 5 min with PBTx before incubation at room temperature in 1 μg/mL DAPI (Invitrogen, ThermoFisher Scientific) solution in PBS for 30 minutes ...
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bioRxiv - Cancer Biology 2023Quote: ... the sample was washed 3 x 5 min with PBS and stained with secondary antibodies Anti-Rabbit-568 (1:500, ThermoFisher A-21069), Anti-Mouse-488 (1:250 ...
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bioRxiv - Immunology 2024Quote: ... Whole- mounts were then washed 3 times for 5 min at room temperature in PBS followed by incubation with Alexa Fluor 568 anti-rat (Invitrogen, 1:500) for 1 hour at room temperature in PBS with 1% BSA and 0.5% Triton-X-100 ...
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bioRxiv - Developmental Biology 2023Quote: ... de-paraffinized and re-hydrated serial sections were subjected to antigen retrieval using 1x PT module buffer 1 (Thermo Scientific) for 36 min at 93°C using a KOS microwave Histostation (Milestone) ...
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bioRxiv - Cancer Biology 2021Quote: ... Samples were subject to reducing SDS-PAGE and immunoblotted using antibodies recognizing recombinant protein (anti-NRG1 NRG1abcam ab180808 1:5000 dilution in 5% milk) or Lamin A (Invitrogen MA1-06101, 1:1000 in 3% BSA).
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bioRxiv - Molecular Biology 2021Quote: ... counted and reseeded in 3 mL A medium (1:3 mix DMEM/F12 (Gibco) and Neurobasal (Gibco) ...
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bioRxiv - Bioengineering 2023Quote: ... and EDC (1-ethyl-3-(3-dimethylaminopropyl) carbodiimide hydrochloride) were purchased from Thermo Fisher Scientific ...
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bioRxiv - Molecular Biology 2021Quote: ... The soluble fraction was then incubated 1h with HisPur Ni-NTA Resin (Thermofisher), and the proteins bound to the resin purified as described before ...
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bioRxiv - Bioengineering 2021Quote: ... was added for 1h and samples were fixed using ProLong Diamond (Thermo Fisher) for at least 24 h ...
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bioRxiv - Cancer Biology 2021Quote: ... and for 1h with rabbit anti-goat alexa647 conjugated secondary antibody (Invitrogen A21446). Images were acquired using Zeiss axioimager Z1 microscope with DAPI ...
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bioRxiv - Cancer Biology 2021Quote: ... followed by 1h incubation with HRP anti-rabbit conjugated secondary antibody (ThermoFisher; #31462) at room temperature under constant agitation ...
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bioRxiv - Microbiology 2024Quote: ... SDM products were digested at 37ºC for 1h with FastDigest DpnI (Thermo Scientific) and transformed into NZY5α competent cells (NZYTech) ...
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bioRxiv - Cancer Biology 2022Quote: ... Proteins separated by their approximate sizes on the gel were then transferred onto nitro-cellular membrane (Thermo Fisher Scientific; 88018). We next blocked the membrane in blocking buffer (Li-Cor ...
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bioRxiv - Cell Biology 2021Quote: ... Cells were blocked in blocking buffer (1% BSA, PBST) for 1h at room temperature and incubated with primary antibody LC3A/B (Thermo Scientific, USA) overnight at 4 °C ...
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bioRxiv - Bioengineering 2021Quote: ... Washed samples were incubated for 1h at RT with the secondary antibody (1:200 Alexa Fluor 647 goat derived anti-rabbit antibody, Thermo Fisher Scientific). Then ...
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Overexpression of HLA class I predominantly on alpha cell in at risk individuals for type 1 diabetesbioRxiv - Immunology 2020Quote: ... for 1h at room temperature (RT) followed by detection using polyclonal goat anti-mouse IgG-Alexa Fluor 647 (Life Technologies, 1:1000) for 30 min at RT ...
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bioRxiv - Biochemistry 2023Quote: ... the slides were incubated with primary antibodies in PBS containing 5% BSA for 2 h and then with the following secondary antibodies for 1h at room temperature: Donkey Anti-Rabbit Alexa 488 (1:1000; Invitrogen; A21206, 2376850) and Donkey Anti-Chicken Alexa 594 (1:1000 ...
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bioRxiv - Cancer Biology 2022Quote: ... diluted at 1/50 or 1/150 followed by an incubation for 1h at room temperature with a secondary goat Alexa FluorTM 488 antibody (ThermoFisher Scientific, #A11029) diluted at 1/1000 ...
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bioRxiv - Bioengineering 2023Quote: ... stained with primary antibody for 1h at 37° C and labeled against F-actin (1:60 in PBS Alexa Fluor® 488 Phalloidin-Invitrogen, # A12379) and DNA (NucBlue ...
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bioRxiv - Cell Biology 2023Quote: ... primary antibodies in 10% GS in 1xPBS overnight at 4°C followed by a 1h incubation with anti-mouse (A11003, Invitrogen, 1:800) secondary antibody in 10% GS in 1xPBS at room temperature ...
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bioRxiv - Neuroscience 2024Quote: ... sections were washed with PBS and incubated for 1h at room temperature in secondary antibody solution containing goat anti-rabbit Alexa Fluor 488 (1:1000, Molecular Probes A11034) and goat anti-mouse IgG1 Alexa Fluor 568 (1:1000 ...
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bioRxiv - Cell Biology 2024Quote: ... incubated in blocking buffer supplemented with 1 µg/ml Hoechst 33342 for 1h at RT with secondary antibodies: Alexa Fluor 647 goat anti-mouse (Invitrogen, A-21236), Alexa Fluor 488 chicken anti-rabbit (Invitrogen ...
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bioRxiv - Plant Biology 2022Quote: 5’-biotinylated RNA and unmodified RNA of Motif 3 (5’-AAAUCGCCGGAG-3’ 5’-Bio-AAAUCGCCGGAG-3’) were synthesized by Eurofins (Hamburg, Germany) and used for LightShift™ Chemiluminescent RNA EMSA Kit (Thermo Scientific). Total protein was extracted from LL and 10 min LL➔HL-treated plants using extraction buffer (25 mM Tris ...
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bioRxiv - Microbiology 2021Quote: ... the hypervariable V3 region of the 16S rDNA gene was amplified from 20 ng of DNA using the primers 5′-CCTACGGGAGGCAGCAG-3′ and 5′-ATTACCGCGGCTGCTGG-3′ (Integrated DNA Technologies BVBA, Leuven, Belgium),(18) 1U Platinum® PCR SuperMix High Fidelity (ThermoFisher Scientific) and 10 μM of primer-mix ...
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bioRxiv - Neuroscience 2022Quote: ... sections were incubated in 0.05 M Tris-HCl buffer (pH 7.6) containing 5 mg 3-3′ diaminobenzidine (Thermo Scientific, TA-060-HDX) per 10 mL buffer and a final concentration of 0.01% hydrogen peroxide for 15 min at RT ...
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bioRxiv - Immunology 2022Quote: ... and hybridized first using RT primer (5′/5Biosg/AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTVN-3′) and then reverse transcribed into cDNA using TSO primer (5′-AAGCAGTGGTATCAACGCAGAGTACATrGrGrG-3’) and RT maxima reverse transcription (Thermo Fisher Scientific). cDNA was amplified using ISPCR primer (5′-AAGCAGTGGTATCAACGCAGAGT-3′ ...
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bioRxiv - Plant Biology 2024Quote: ... The purified RNA was reverse transcribed using the CLIP-3 PCR primer (5’-GTCTCGTGGGCTCGG-3’) and SuperScript IV reverse transcriptase (18090010; Thermo Fisher Scientific). The first-strand cDNA was then subjected to PCR using the CLIP-5 PCR primer (5’-TCGTCGGGCAGCGTC-3’) ...
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bioRxiv - Microbiology 2024Quote: ... total RNA was harvested from a 10 ml parasite culture (5% hematocrit; >3% parasitaemia) by resuspending the pelleted RBCs in 3 ml pre-warmed Trizol (Invitrogen #15596-018). After a 5 min incubation step at 37°C ...
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bioRxiv - Cell Biology 2020Quote: ... or DiD (1,1’-dioctadecyl-3,3,3’,3’-tetramethylindodicarbocyanine perchlorate, aka DiIC18(5)) (Thermo Fisher #D307) at a final concentration of 4 μg/mL ...
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bioRxiv - Cell Biology 2020Quote: ... Cells were washed 3 times in 5% Bovine Serum Albumin (BSA; ThermoFisher Scientific, 11423164) in PBS then incubated with primary antibody to H2AX (Ser139) ...
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bioRxiv - Cell Biology 2021Quote: ... 5 μg mtDNA were digested with 3 ul DraI Fastdigest restriction enzyme (Thermo Scientific) at 37°C for 3h and extracted with 1 volume phenol-chloroform ...
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bioRxiv - Microbiology 2022Quote: ... FAM-5=CAT CCA ATC AAA GAC ATT GCG A 3=-TAMRA (Life Technologies).Viral RNA was detected using the CFX96 detection system (Bio-Rad Laboratories ...
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bioRxiv - Neuroscience 2021Quote: ... and 2-(4-Iodophenyl)-3-(4-nitrophenyl)-5-phenyltetrazolium Chloride (INT, #I00671G, Fisher Scientific).
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bioRxiv - Cell Biology 2021Quote: ... Coverslips were washed 3 × 5 min and mounted on slides using Prolong Gold (Invitrogen). Deconvolution wide-field microscopy was performed using the DeltaVision Elite system equipped with an NA 1.42 60x PlanApo objective (Olympus ...
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bioRxiv - Cell Biology 2023Quote: ... The following siRNA target sequences were used: GTPBP5 siRNA: 5’-CGGUGGACACGUCAUUCUGTT-3’ (134621, Ambion); GTPBP7 siRNA ...
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bioRxiv - Cell Biology 2022Quote: ... CCDC66 was depleted using an siRNA with the sequence 5′-CAGTGTAATCAGTTCACAAtt-3′ (Thermo Scientific). Silencer Select Negative Control No ...
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bioRxiv - Cell Biology 2024Quote: ... si-PRELID1: 5’-CCCGAAUCCCUAUAGCAAA-3’) were transfected using Lipofectamine RNAiMAX (Thermo Fisher Scientific, 13778075). Assays were normally carried out 48 h after transfection ...