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Citations for Thermo Fisher :
901 - 950 of 10000+ citations for 5 Nitro 1H benzo de isoquinoline 1 3 2H dione since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2022Quote: ... NIM was exchanged daily for 3:1-DMEM/F12-medium (3 parts DMEM (Thermo Fisher Scientific, #31966047) and one-part F12 medium (Ham’s F-12 Nutrient Mix ...
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bioRxiv - Neuroscience 2022Quote: ... washed with PBS-T and then incubated with secondary antibodies for 2h (donkey anti-rabbit lgG Alexa Fluor 488, Invitrogen, Thermo Fisher, A21206; donkey anti-Mouse lgG Alexa Fluor 555, Invitrogen, Thermo Fisher, A21202, 1:600 in blocking buffer). Sections were washed twice with PBS before being mounted onto gelatin subbed slides ...
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bioRxiv - Neuroscience 2023Quote: ... Sections were washed with PBS 3-5 times for 5 minutes each and mounted with ProLong Gold Antifade Mountant (#P36930, Thermo Fisher Scientific, Waltham MA). Confocal images were taken with the 20x and 40x (oil ...
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bioRxiv - Immunology 2024Quote: ... and OtsuR747 (5’- TATGCCTGAGTAAGATACGTGAATGGAATT-3’) primers (IDT, Coralville, IA) and detected with the probe OtsuPr665 (5’-6FAM- TGGGTAGCTTTGGTGGACCGATGTTTAATCT-TAMRA) (Applied Biosystems, Foster City, CA) by SsoAdvanced Universal Probes Supermix (Bio-Rad ...
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bioRxiv - Neuroscience 2021Quote: ... anti-sense, 5’ AAGAGGUUUAGCUGGUAC CTT 3’) or control nontargeting siRNA (Genepharma, Shanghai) using Lipofectamine RNAiMAX (Invitrogen). Six hours after transfection ...
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bioRxiv - Immunology 2021Quote: ... Samples were injected onto a Biobasic AX LC column (5 μm, 50 × 3 mm; Thermo Scientific). The mobile phase consisted of 100 mM ammonium carbonate (A ...
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bioRxiv - Genomics 2020Quote: ... and fragmented by heating at 94 °C for 3 min in 5× First Strand Buffer (Invitrogen). The fragmented mRNAs were reversely transcribed into cDNA by SMARTScribe reverse transcriptase (TaKaRa) ...
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bioRxiv - Cancer Biology 2020Quote: The 3’ & 5’RACE products were cloned using TOPO TA Cloning Kit (Invitrogen, Cat #K4530-20), following the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2022Quote: ... passage 3 fibroblasts were trypsinized 5 min at 37°C using 0.05% trypsin with EDTA (Gibco), centrifuged at 1000 rpm for 5 min at RT and resuspended in HBSS (Fisher Scientific ...
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bioRxiv - Cancer Biology 2022Quote: ... DM-3 and RS-5 cells were cultured in NCTC-109 medium (Gibco, Carlsbad, CA, USA) supplemented with antibiotics ...
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bioRxiv - Cell Biology 2022Quote: ... C12 (4,4-Difluoro-5-Methyl-4-Bora-3a,4a-Diaza-s-Indacene-3-Dodecanoic Acid) (Invitrogen) at 2 mg/ml in PBS for 10 min or with Laurdan (see above ...
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bioRxiv - Genomics 2020Quote: ... commercially available CRISPR control targeting HPRT (IDT) and KANK1 exon-targeting (ThermoFisher Scientific, 5’-GUCUAGUUGAUAACCAUAGG-3’) gRNAs were also obtained ...
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bioRxiv - Microbiology 2021Quote: ... 40 μg/mL 5-bromo-4-chloro-3-indolyl-beta-D-galactopyranoside (X-Gal) (Thermo Scientific) and 1 mM isopropyl beta-D-thiogalactopyranoside (IPTG ...
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bioRxiv - Molecular Biology 2021Quote: ... Coverslips were washed 3 x 5 minutes in PBST and then incubated with secondary antibodies (Invitrogen) diluted in PBST + 0.2% BSA for 1 hour at room temperature ...
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bioRxiv - Bioengineering 2021Quote: A 3 or 5 wt% solution of GelMAL was prepared by dissolving GelMAL in PBS (Gibco Invitrogen ...
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bioRxiv - Neuroscience 2023Quote: ... PBS washes (3 × 5 min) and incubation with primary antibody (mouse anti-GFP, A11120, Life technologies; 1:200 ...
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bioRxiv - Physiology 2023Quote: ... R: 5’ TGCCTTCGTGCCGTACTG 3’) and confirmed by DNA sequencing following sub-cloning into pCRTM-Blunt (Invitrogen). Further genotypes were determined by real-time PCR with specific probes designed for each gene (Transnetyx ...
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bioRxiv - Molecular Biology 2022Quote: ... qPCR was carried out on a QuantStudio 3 or QuantStudio 5 Real-Time PCR System (ThermoFisher) with a passive reference of ROX using the following cycling conditions ...
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bioRxiv - Developmental Biology 2022Quote: ... Cells were passaged approximately every 3-5 days (70-85% confluency) using Accutase (Gibco, #11105-01) to detach cells ...
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bioRxiv - Cancer Biology 2024Quote: ... plasmid containing a sgRNA targeting APC (5‘-GGCACTCAAAACGCTTTTGA-3‘) using lipofectamin-2000 (Thermo Fisher Scientific, 11668027). WT organoids were grown in WENR medium and APC truncated organoids were grown in ENR medium and are later refererred to as mCol WT and mCol APC ...
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bioRxiv - Genetics 2023Quote: ... and the random primer 5′-GTTTCCCAGTCACGATCNNNNNNNNNNNNNNN-3′ for the RT step and recombinant Taq polymerase (Invitrogen) for the PCR step using D388 specific primers 5′-GAGAGAAGGATTAGATTGTG-3′ and 5′-CCTGTATAATAGAAGTACCA-3′ ...
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bioRxiv - Physiology 2023Quote: ... were incubated for 20 minutes with 5 µM of Di-3-ANEPPDHQ (Thermo Fisher Scientific, USA) membrane dye ...
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bioRxiv - Neuroscience 2022Quote: ... washed 3 × 5 min with TBS and embedded in ProLongTM Gold Antifade Mountant (Thermo Fisher Scientific). Images were taken with a Nikon Eclipse90i microscope and acquisition was performed with the NIS Elements software (Version 5.01) ...
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bioRxiv - Cancer Biology 2022Quote: ... 100 nM (final concentration) shRNA or 3 ∼ 5 µg plasmid in 50 µL Opti-MEM (Gibco) was mixed with 5 µL transfection reagent (Lipofectamine ...
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bioRxiv - Microbiology 2022Quote: ... Data analysis was performed with the Quantstudio 3&5 Design and analysis software (Applied Biosystems, v1.5.1). Cq values were obtained via automated threshold determination ...
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bioRxiv - Neuroscience 2023Quote: ... PBS washes (3 × 5 min) and incubation with primary antibody (mouse anti-GFP, A11120, Life technologies; 1:200 ...
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bioRxiv - Microbiology 2024Quote: ... Amplifications were achieved using a 3-step program on a QuantStudio 5 system (Thermo Fisher Scientific). Transcript abundance was calculated using the ΔΔCT method (75 ...
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bioRxiv - Neuroscience 2024Quote: ... Slices were subsequently washed 3 x 5 minutes in PBST 0.01% (DPBS ThermoFisher, Tween P1379 Sigma), permeabilised for 15 minutes in PBST 0.5% and washed in PBST 0.01% a further three times ...
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bioRxiv - Neuroscience 2024Quote: ... Slides were then washed for 5 minutes 3 times in HistoClear (50-899-90147, Fisher Scientific) and briefly dried before the addition of Permount mounting media (SP15-100 ...
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bioRxiv - Cancer Biology 2024Quote: ... and were passaged in aggregates every 3-5 days using Versene solution (Thermo Fisher Scientific, #15040066) according to the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2024Quote: ... iPSCs were split by incubating for 3-5 min at +37 L in EDTA (15575020, Invitrogen) and kept in 5 µM Y-27632 2HCl (S1049 ...
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bioRxiv - Developmental Biology 2024Quote: ... then blocked with blocking buffer (3% bovine serum albumin with 5% normal goat serum (Gibco, 16210064), and 5% normal donkey serum (Sigma-Aldrich ...
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bioRxiv - Molecular Biology 2022Quote: ... pDNOR207-ZNF451-1 (isoform 1) and pDNOR207-ZNF451-3 (isoform 3) were generated using the Gateway® cloning BP reaction (Thermo Fisher Scientific) upon cDNA amplification using BP-tailed primers and pDNOR207 as donor vector ...
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bioRxiv - Neuroscience 2022Quote: ... the medium was replaced every 2-3 days and cells passaged 1:2 or 1:3 weekly with 0.25% Trypsin/EDTA (Thermo Fisher Scientific, #25200-056) pre-warmed at 37°C.
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bioRxiv - Cancer Biology 2023Quote: ... Unbound primary antibody was washed three times in PBS and secondary Alexa-conjugated antibodies were used to detect the signal (ThermoFisher, 1:1000, 1h at RT). Finally nuclei were labelled with Hoechst 33258 (1 μg/ml ...
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bioRxiv - Cell Biology 2022Quote: ... 3 ml was diluted 1:1 with PBS (Thermo Fisher Scientific, Waltham, MA) and kept on ice for purity analysis by flow cytometry ...
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bioRxiv - Plant Biology 2020Quote: ... The cDNA was quantified in a Nanodrop-2000 spectrophotometer (Thermo Scientific, Wilmington DE, USA).
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bioRxiv - Plant Biology 2020Quote: ... then its concentration was measured by a Nanodrop 2000 spectrophotometer (Thermo Scientific, Wilmington, DE). Serial dilutions were prepared by adding 10 μl of the original amplicon to 90 μl of nuclease free water for each case ...
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bioRxiv - Developmental Biology 2021Quote: ... RNA purity was determined using a NanoDrop 1000 (Thermo Fisher Scientific, Wilmington, DE, USA). Reverse transcription was performed using a NovoScript® 1st Strand cDNA Synthesis Kit (Novoprotein ...
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bioRxiv - Evolutionary Biology 2020Quote: ... Following DNA quantification using a Qubit fluorometer (Invitrogen, Thermo Fisher Scientific, Wilmington, DE, USA), individuals were pooled to equal molarity without PCR-enrichment to obtain a single DNA library per sampling site (and per time point in the case of the marsh site M1) ...
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bioRxiv - Genomics 2021Quote: ... RNA quantity and quality were assessed by NanoDrop 1000 spectrophotometer (Thermofisher Scientific, DE, UK), and TapeStation (Agilent Technologies ...
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bioRxiv - Developmental Biology 2021Quote: ... The quantity and quality of RNA were verified with NanoDrop 1000 (Thermo Scientific, DE). RNA-Seq analysis was performed at GENEWIZ following the manufacturer’s protocol ...
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bioRxiv - Physiology 2021Quote: ... Samples were de-salted using SOLAμ SPE plates (Thermo Scientific, Cat no 60109-103). Columns were equilibrated using 100% acetonitrile (ACN) ...
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bioRxiv - Molecular Biology 2021Quote: ... The isolated DNA was quantified spectrophotometrically using a Nanodrop 2000c (Thermo Scientific, Wilmington, DE). Assessment of the quality of the isolated DNA was performed by electrophoresis on 0.8% agarose gels stained with SYBR™ Green I Nucleic Acid Gel stain (Invitrogen ...
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bioRxiv - Microbiology 2020Quote: ... the samples were quantified via NanoDrop® spectrophotometry (Thermo Fisher Scientific; Wilmington DE, USA). Normalization and sequencing of the library pool was performed at the Agriculture and Food Laboratory ...
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bioRxiv - Plant Biology 2020Quote: The peptide was analysed using EASY-nLC 1000 (Thermo Fisher Scientific, Wilmington, DE, USA) coupled with Orbitrap Fusion mass spectrometry (Thermo Scientific ...
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bioRxiv - Physiology 2020Quote: ... The concentration and purity were determined using NanoDrop 2000 (Thermo Fisher Scientific, Wilmington, DE) and cDNA was synthesized from total RNA using the iScript cDNA Synthesis Kit (Bio-Rad ...
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bioRxiv - Physiology 2020Quote: ... The concentration and purity were determined using NanoDrop 2000 (Thermo Fisher Scientific, Wilmington, DE) and cDNA was synthesized from total RNA using the iScript cDNA Synthesis Kit (Bio-Rad ...
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bioRxiv - Developmental Biology 2021Quote: ... DE differentiation was then carried out in RPMI-1640 media (Thermo Fisher; 11875-093) supplemented with non-essential amino acids (ThermoFisher ...
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bioRxiv - Microbiology 2020Quote: ... the quality of DNA was measured using NanoDrop™ one (Thermo Fisher Scientific, DE) and quantified using Qubit Fluorometer 3.0 (Invitrogen ...