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Citations for Thermo Fisher :
6401 - 6450 of 9427 citations for Vertical Gel Casting Cassettes since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2021Quote: ... Samples were incubated at 95°C for 10 minutes before loading on 12-15% precast NuPAGE gels (Invitrogen).
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bioRxiv - Neuroscience 2020Quote: ... Aliquots representing 40 μg total proteins were separated on Novex™ 4-20% Tris-Glycine Mini Gels (Invitrogen) or 4-20% Criterion™ TGX Stain-Free™ Protein gels (Bio-Rad ...
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bioRxiv - Neuroscience 2020Quote: ... was added to samples prior to being loaded onto NuPAGE™ 4-12% Bis-Tris Protein Gels (Invitrogen). The separated proteins were subsequently transferred to a PVDF membrane (Bio-Rad Laboratories ...
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bioRxiv - Bioengineering 2021Quote: ... The DNA fragments from PCR and restriction digestion were recovered from agarose gels using GeneJET kits (Thermo Scientific). For ligations ...
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Therapeutic base and prime editing of COL7A1 mutations in recessive dystrophic epidermolysis bullosabioRxiv - Bioengineering 2021Quote: ... equal amounts of proteins from each group were loaded onto Nupage Novex Bis-Tris Gels (Thermo Fisher Scientific), and electrophoresis was performed using an X-cell SureLock Mini-Cell (Thermo Fisher Scientific) ...
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bioRxiv - Bioengineering 2020Quote: ... The stacked gel setup was sandwiched between two pieces of western blotting filter paper (Thermo Fisher Scientific 84783) held together using a laser-cut acrylic holder with a cut-out for the gel stacks ...
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bioRxiv - Biochemistry 2020Quote: ... Lysates were electrophoresed through either 4-12% NuPAGE or 8% E-PAGE gels (Thermo Fisher Scientific, MA, USA) and transferred to nitrocellulose by iBlot 2 dry blotting system (Thermo Fisher Scientific ...
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bioRxiv - Biophysics 2021Quote: ... The quality of each protein preparation was assessed by NuPAGE Novex 4–12% Bis-Tris protein gels (ThermoFisher). Gels were stained with Coomassie Brilliant Blue R-250 (ThermoFisher ...
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bioRxiv - Neuroscience 2020Quote: ... NuPAGE Bis-Tris precast gels and Phosphate buffered saline (PBS) were purchased from Life Technologies (Grand Island, NY). Bicinchoninic Acid (BCA ...
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bioRxiv - Cell Biology 2021Quote: ... Samples were loaded onto a Novex NuPAGE SDS-PAGE gel system with MOPS running buffer (Life Technologies, #NP001). Proteins were transferred onto nitrocellulose membranes and blotted with indicated antibodies ...
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bioRxiv - Cell Biology 2021Quote: ... The elution was analyzed via SDS-PAGE on a gel stained with SYPRO™ Red (Thermo Fisher Scientific).
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bioRxiv - Cancer Biology 2020Quote: ... Equal volumes of the lysates were loaded into each well of Bis-Tris precast polyacrylamide gels (Thermo Fisher). Antibodies used are listed in Table S3.
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bioRxiv - Cancer Biology 2020Quote: ... The protein samples were size fractionated on Novex 4-20% Tris-Glycine Mini Gels (Thermo Fisher Scientific XP04200BOX) at room temperature and subsequently transferred overnight onto Immuno-Blot PVDF membranes (BioRad 1620177 ...
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bioRxiv - Cancer Biology 2020Quote: ... Equal amounts (50 μg) of proteins were loaded and fractionated on 4–12% NuPAGE Bis-Tris Gels (Invitrogen). Protein were transferred to PVDF membranes (Millipore ...
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bioRxiv - Cell Biology 2021Quote: ... Gels were washed with 10 mM EDTA and then blotted on a dry blotting transfer system (iBlot2, Invitrogen). Whi5-V5 was detected with a commercial Anti-V5 mouse antibody (Bio-Rad ...
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bioRxiv - Plant Biology 2021Quote: ... Proteins were transferred onto PVDF membranes for 10 min at 25 V using iBLOT gel transfer system (Invitrogen). After 30 min in 5% saturation solution (50 g L-1 milk ...
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bioRxiv - Biochemistry 2021Quote: ... The gel band corresponding to the cross-linked complex was excised and digested with trypsin (Thermo Scientific Pierce) [107] and the resulting tryptic peptides were extracted and desalted using C18 StageTips [108] ...
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bioRxiv - Biochemistry 2020Quote: ... Total proteins were detected in the gel by silver staining using the Pierce Silver Stain Kit (Life Technologies). Gels were imaged using a Syngene imager (Cambridge ...
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bioRxiv - Bioengineering 2021Quote: 500 μg of RNA was extracted from hBMMSC-seeded ADC gels (n = 3) using TRIzol reagent (Invitrogen, USA) as per manufacturer’s instructions following the initial 48 h of mechanical stimulation ...
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bioRxiv - Biochemistry 2020Quote: ... Intermolecular G4 formation was confirmed by native gel electrophoresis followed by staining with SYBR® Gold (Life Technologies), as described (34) ...
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bioRxiv - Cell Biology 2021Quote: ... All pellets were boiled with samples buffer and run into a 4-12% NuPAGE gradient gel (Invitrogen, NP0322BOX). Gels were stained with Coomassie blue (Bio-Rad ...
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bioRxiv - Microbiology 2020Quote: ... 10 µl of the sample was then resolved on a 4-12% bis-trisNuPage gel (Thermo Fisher Scientific) with 1X MOPS SDS gel buffer (Thermo Fisher Scientific ...
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bioRxiv - Microbiology 2020Quote: ... BN-PAGE gels were stained using the Colloidal Blue Staining Kit according to the manufacturer’s instructions (Life Technologies). For SDS PAGE ...
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bioRxiv - Microbiology 2020Quote: ... Coomassie blue staining of SDS-PAGE gels was performed using the PageBlue Protein Staining Solution (Thermo Fisher Scientific).
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bioRxiv - Microbiology 2020Quote: ... Proteins in parasite lysates were resolved NuPAGE using 4-12% gradient bis-Tris polyacrylamide gels (Thermo Fisher Scientific). Separated proteins were then transferred from the gels to a nitrocellulose membrane ...
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bioRxiv - Plant Biology 2020Quote: ... DNA quality was assessed by agarose gel electrophoresis and quantified using the Qubit dsDNA BR Assay Kit (Thermofisher). Whole genome libraries for use in bait capture were prepared using Ion Plus Fragment Library kits according to the manufacturer’s instructions with a target insert size of 400bp and unique Ion Xpress barcodes for each sample ...
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bioRxiv - Plant Biology 2021Quote: ... RNA integrity was visualized in 0.9% agarose gels stained with Sybr™ Safe (Thermo Fisher Sci. Cat. #S33102). One microgram of total RNAs from each sample was treated with DNAse (Thermo Fisher Sci ...
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bioRxiv - Plant Biology 2020Quote: ... and afterwards digested overnight at 37 °C with trypsin (Sigma; in-gel tryptic digestion kit, Thermo Fisher Scientific), desalted with C18 Ziptips (Millipore) ...
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bioRxiv - Plant Biology 2021Quote: ... Equal amounts of total protein were loaded and separated on Nu-PAGE 4-12% Bis-Tris gels (Invitrogen). Proteins were immune-detected using anti-IRT1 (Agrisera ...
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bioRxiv - Microbiology 2021Quote: ... Samples were separated on Bolt 4–12% Bis-Tris gels in Bolt MOPS SDS Running Buffer (Thermo Scientific) and proteins were transferred onto a PVDF membrane (BioRad ...
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bioRxiv - Developmental Biology 2021Quote: Whole-cell lysates and immunoprecipitated proteins were subjected to NuPAGE 4%-12% bistris acrylamide gel electrophoresis (Life Technologies). Using standard protocols ...
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bioRxiv - Neuroscience 2020Quote: ... 20μL of protein samples were run on 4-20% gradient tris-glycine gels (Invitrogen, Gand Island, NY, USA) for 120min at 130V and were transferred onto nitrocellulose membranes overnight at 20V ...
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bioRxiv - Microbiology 2021Quote: ... & 0.008% Triton x-100 (v/v))(45) containing 0.2 µL/mL SYBR Green I Nucleic Acid Gel Stain (10,000x in DMSO; ThermoFisher) was added to each well and incubated for 1 h at RT before fluorescence intensity was read on a Glomax® Explorer Fully Loaded (Promega ...
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bioRxiv - Cancer Biology 2021Quote: ... 20-25 μg of proteins were separated using 4-12% gradient NuPAGE Novex Bis-Tris gel (Life technologies). Protein transfer was performed on methanol-activated PVDF or Nitrocellulose membrane ...
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bioRxiv - Microbiology 2021Quote: ... The gel was washed twice with water for 5 min followed by incubation with renaturation buffer (ThermoFisher, #LC2670) for 21 h at 30° C ...
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bioRxiv - Microbiology 2020Quote: ... Samples were re-boiled and electrophoresed using the tricine buffer system with Novex tricine 16%-acrylamide gels (Invitrogen). Spectra Multicolor Low Range Protein Ladder (Thermo ...
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bioRxiv - Molecular Biology 2021Quote: ... The DNA integrity was verified using agarose gel (1%) electrophoresis stained with 0.5x SYBR® Green (Invitrogen®) and observed under UV light ...
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bioRxiv - Molecular Biology 2021Quote: ... The amplified products were separated by electrophoresis in an agarose gel (1%) stained with 0.5x SYBR Green (Invitrogen).
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bioRxiv - Microbiology 2019Quote: ... purified using PureLink™ Quick Gel Extraction and PCR Purification Combo Kit (Thermo Fisher Scientific, Carlsbad, California, USA) and finally quantified using a Qubit® Fluorometer (Thermo Fisher Scientific ...
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bioRxiv - Microbiology 2020Quote: ... Thirty µl of the lysate was loaded immediately onto a precast 10% Tris-Bis NuPAGE gel (Invitrogen, USA) using 1 x MOPS solution (Invitrogen ...
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bioRxiv - Genomics 2020Quote: ... and separated by electrophoresis in MOPS buffer at 200 V in a Bolt gel tank (Thermo Scientific #A25977) according to manufacturer instructions ...
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bioRxiv - Cancer Biology 2021Quote: ... Gels were gently transferred to 24-wells and cultured in 1ml Medium-199 with insulin-transferrin-selenium (Gibco). Adipocyte gels were used for experiments with other cell types within 7 days of isolation ...
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bioRxiv - Cancer Biology 2021Quote: ... was made for 3D mono- and co-cultures mixing (per 100μl gel) 34μl of 3mg/ml rat-tail collagen I (Gibco), 4μl of 10x DMEM low-glucose (Sigma) ...
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bioRxiv - Cancer Biology 2020Quote: ... The slides were dried at 37°C and stained with SYBR gold nucleic acid gel stain (Thermofisher Scientific). Slides were mounted with Vectashield antifade mounting media (Vector laboratories ...
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bioRxiv - Neuroscience 2020Quote: ... 15-20ug of protein from each sample was loaded on 4-12% Bolt Bis-Tris gradient gels (Invitrogen), followed by semi-dry transfer onto nitrocellulose membranes ...
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bioRxiv - Cell Biology 2021Quote: ... The lysates were subjected to electrophoresis on NuPAGE 4% to 12% Bis-Tris gels (NP0321; Thermo Fisher Scientific). Proteins were transferred to polyvinylidene fluoride membranes and probed using anti-p62 (SQSTM1 ...
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bioRxiv - Cell Biology 2020Quote: ... samples were boiled for 5 min before analysis by SDS-PAGE gels stained with SimplyBlue Safe Stain (Invitrogen).
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bioRxiv - Cell Biology 2020Quote: ... Samples were boiled 5 min at 95 °C and loaded in pre-cast Novex Tris-Glycine gels (Invitrogen). Pre-cast gels were run in an XCell SureLock™ Mini-Cell Electrophoresis tank (ThermoFisher ...
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bioRxiv - Cell Biology 2020Quote: Proteins were separated using NuPAGE 4 to 12% Bis-Tris gels and transferred onto nitrocellulose membranes (Life Technologies). The blots were blocked with 5% nonfat dry milk at room temperature for 1 h and incubated overnight at 4°C with desired primary antibodies at concentration per the manufacturer’s protocol ...
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bioRxiv - Biophysics 2020Quote: ... The resulting gels were stained with ethidium bromide for 30 minutes (0.5 μg/mL ddH20, Thermo Fisher Scientific), washed in ddH20 (30 minutes) ...