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Citations for Thermo Fisher :
6551 - 6600 of 10000+ citations for Biotin Z Antibody Internalization Kit rabbit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2019Quote: The digested sticky ends were filled up with 10mM dNTPs (without dATP) and 0.4mM biotin-14-dATP (Life Technologies) and 40U DNA Pol I ...
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bioRxiv - Genomics 2020Quote: ... the restriction fragment overhangs were filled in and the DNA ends were marked with biotin-14-dATP (Life Technologies). The biotin-labeled DNA was sheared and pulled down using Dynabeads MyOne Stretavidin T1 beads (Life Technologies ...
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bioRxiv - Cell Biology 2019Quote: Biotinylated sense (negative control) and antisense biotin-labeled DNA oligomers corresponding to GUARDIN (1μg) were coupled to streptavidin-coupled Dynabeads (Invitrogen). The Dynabeads were subsequently incubated with cell lysates for 4 h and eluted proteins subjected to SDS–PAGE and staining with Coomassie Brilliant Blue G-250 ...
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bioRxiv - Genetics 2021Quote: ... Newly synthesized transcripts were selected for biotin-16-UTP incorporation using streptavidin coated magnetic beads (ThermoFisher, Dynabeads M-280). Beads were extracted using QIAzol reagent ...
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bioRxiv - Pathology 2020Quote: ... intratesticular injection of EZ-Link Sulfo-NHS-LC-Biotin (15μl, 7.5mg/mL) was performed in the left testis (21335, Thermo Scientific). Testes were harvested 30 min after injection and fixed in 4% PFA ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... The liganded beads were blocked with excess biotin and washed with SEA BLOCK Blocking Buffer (Thermo Fisher, Rockford, IL), 1 % BSA ...
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bioRxiv - Bioengineering 2019Quote: ... was biotinylated using a 5:1 molar excess of Ez-Link™ Sulfo-NHS-LC-Biotin (Thermo-Fisher Scientific) overnight at 4°C ...
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bioRxiv - Genomics 2021Quote: 40-80 ng of each probe (labeled with biotin or Dig) and 1µg of unlabeled human COT1-DNA (Invitrogen) were dissolved in 4µl of hybridization buffer (10% dextran sulfate ...
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bioRxiv - Biochemistry 2021Quote: ... Glass slides were treated with 3-aminopropyl triethoxysilane and passivated with a biotin-PEG SVA/mPEG SVA mix (Invitrogen). Treated glass slides were assembled with double sided tape and an aluminum backing to generate flow channels in a custom microscope slide ...
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bioRxiv - Developmental Biology 2021Quote: ... Digestion generated overhangs were filled in with a mixture of 0.4mM biotin-14-dCTP (10 mL; Thermo Fisher Scientific) and 10mM dATP/dGTP/DTTP (0.5 mL each ...
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bioRxiv - Neuroscience 2021Quote: ... then cells were placed on ice and incubated with 1.5 mg/ml Sulfo-NHS-SS-biotin (ThermoFisher Scientific, #21331) for 1 h ...
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bioRxiv - Cell Biology 2021Quote: Cortical neuronal cultures were washed three times with ice-cold PBSCM (PBS + 1mM MgCl2 + 0.1 mM CaCl2) and incubated with 0.5 mg/ml Sulfo-NHS-SS-biotin (Life Technologies) for 1 hour at 4°C ...
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bioRxiv - Genomics 2021Quote: ... 0.5μM oligo-dT (IDT; 100uM 5′-biotin-ACGAGCATCAGCAGCATACGA-T30VN-3′) and 0.5mM dNTPs/each (Thermo Fisher; 25 mM each) and snap frozen at −80 °C ...
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bioRxiv - Cell Biology 2022Quote: ... or with Biotin-SP (1:100; Jackson Immuno Research) followed by fluorescent conjugates of streptavidin 647 (1:500; Invitrogen) in 0.1M DPBS ...
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bioRxiv - Cell Biology 2022Quote: ... EC were washed three-times with ice-cold PBS and incubated with 0.3 mg/ml EZ-link Sulfo-NHS-LC-Biotin (ThermoFisher) in PBS containing 2 mM MgCl2 and 2 mM CaCl2 on ice for 30 minutes with gentle agitation ...
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bioRxiv - Biophysics 2022Quote: ... Equal aliquots of RBCs were then mixed with various concentrations of EZ-Link Sulfo-NHS-LC-Biotin (Thermo Scientific) at a pH of 7.2 for 30 min at room temperature ...
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bioRxiv - Cell Biology 2022Quote: ... cell surface proteins were biotinylated with 0.5 mg/ml Ez-link sulfo-NHS-SS-Biotin (21331, Thermo Fisher Scientific) in PBS containing 1 mM CaCl2 and 0.5 mM MgCl2 (PBS/CM ...
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bioRxiv - Synthetic Biology 2022Quote: The RBDs were biotinylated by amine coupling with sulfo-NHS-SS-Biotin according to the manufacturer’s manual (Thermo Fisher). Briefly ...
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bioRxiv - Neuroscience 2022Quote: ... Approximately 1 µl of a 12 mM solution of dextran (3kDa, tetramethylrhodamine and biotin labeled, D7162, Thermo Fisher Scientific) in sterile DMSO was injected using a pulled glass pipette (tip diameter ∼30 μm ...
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bioRxiv - Immunology 2022Quote: ... Equal aliquots of RBCs were then mixed with various concentrations of EZ-Link Sulfo-NHS-LC-Biotin (Thermo Scientific) at a pH of 7.2 for 30 min at room temperature ...
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bioRxiv - Cell Biology 2022Quote: ... cells (5×106/0.5 ml) were labeled with 100 μg Immunopure Sulfo-NHS-LC-Biotin (Thermo Scientific, Rockford, IL) for 30 min at 22 °C ...
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bioRxiv - Molecular Biology 2024Quote: ... DNA purification and enrichment of biotin-labeled DNA with Dynabeads™ M-280 streptavidin (Thermo Fisher Scientific, Cat# 11205D). The enriched bead-bound DNA was subjected to end repair ...
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bioRxiv - Molecular Biology 2024Quote: ... The biotin-16-UTP-labelled NEAT1_1 IVT product (1 µg) was immobilized on 25 µL streptavidin C1 dynabeads (Invitrogen), washed twice with 800 µL IP buffer and incubated (2 h ...
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bioRxiv - Neuroscience 2024Quote: ... The coding sequences of a five glycine linker and intracellular GFP and biotin tags followed and were inserted in a pcDNA3.1(-)/myc-His (Invitrogen) vector backbone ...
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bioRxiv - Microbiology 2024Quote: ... both RBD and HEL were site-specifically biotinylated in house using EZ-link maleimide-PEG2-biotin (ThermoFisher, CAT#A39261) so that the binding epitopes on these proteins were not modified ...
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bioRxiv - Cell Biology 2024Quote: ... 0,5µM Smartseq3 OligodT30VN (IDT; 5’-Biotin-ACGAGCATCAGCAGCATACGAT30VN-3’) adjusted to RT volume and 0,5mM dNTPs/each (Thermo Fisher, #R0181). After cell sorting lysis plates were centrifuged before storage at -80°C ...
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bioRxiv - Cancer Biology 2022Quote: ... Cells were then incubated with EZ-Link™ Sulfo-NHS-SS-Biotin (0.5 mg ml-1, #21331; Thermo Fisher) for 30 min at 4°C ...
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bioRxiv - Neuroscience 2022Quote: ... supplemented with 2 mM MgCl2 and 0.1 mM CaCl2 (PBSCM) and incubated in 0.5 mg/mL Sulfo-NHS-LC-Biotin (Thermo Fisher) dissolved in PBSCM at 4°C for 10 minutes ...
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bioRxiv - Molecular Biology 2023Quote: ... The membrane was blocked with 5% bovine serum albumin in TBST and biotin was detected with Streptavidin-HRP (ThermoFisher).
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bioRxiv - Immunology 2023Quote: ... cerevisiae was subjected to biotinylation in 1 mg/ml EZ-Link™ Sulfo-NHS-SS-Biotin (Thermo Scientific #21331) for 1h at RT with rotation ...
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bioRxiv - Biophysics 2023Quote: ... Protein at 2 mg/mL in interaction buffer (150 mM NaCl 50 mM Phosphate pH 8) was treated with 1.25 mM Biotin LC-Hydrazide (Thermofisher) and 5 mM EDC for two hours then dialyzed against the initial buffer ...
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bioRxiv - Genomics 2023Quote: ... Biotin pull down was initiated py pipetting 20 µL Dynabeads My-One Streptavidin T1 beads (ThermoFisher Scientific, Cat # 65306) into DNA lo-bind tubes ...
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bioRxiv - Microbiology 2023Quote: ... a biotin-labeled 64-nt single-stranded DNA and the Century-Plus RNA ladder (Thermo Fisher Scientific, MA, USA) were loaded to an 8% polyacrylamide gel and electrophoresed in 1× TBE buffer ...
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bioRxiv - Bioengineering 2023Quote: ... Biotinylation of the cell surface was performed by adding 1 mM Sulfo-NHS-LC-Biotin (Thermo Fisher, Waltham, MA) to the cell pellet at 250 μL per 1 million cells ...
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bioRxiv - Cell Biology 2023Quote: ... Cell surface proteins were subsequently labelled with 0.3 mg/ml EZ-Link™ Sulfo-NHS-SS-Biotin (Thermo Scientific) at 4 °C ...
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bioRxiv - Genomics 2023Quote: ... Biotin pull down was initiated py pipetting 20 μL Dynabeads My-One Streptavidin T1 beads (ThermoFisher Scientific, Cat # 65306) into DNA lo-bind tubes ...
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bioRxiv - Genomics 2023Quote: Fragment ends were filled in by adding 37.5 μL of 0.4 mM biotin-14-dATP (ThermoFisher Scientific, # 19524-016), 1.5 μL each of 10 mM dCTP ...
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bioRxiv - Bioengineering 2023Quote: ... free NHS-AF647 was eliminated with 0.5mL 7kDa MWCO Zeba Dye and Biotin Removal Spin Columns (Thermo Fisher Scientific). Conjugated antibodies were used immediately or stored at 4°C ...
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bioRxiv - Cell Biology 2024Quote: ... was coated for 1 hr at room temperature with the CaptureSelect™ Biotin anti-AAV9 conjugate (Thermo Fisher Scientific), at a dilution of 1:10,000 in PBST ...
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bioRxiv - Immunology 2024Quote: Zymosan particles were then washed twice with 100 mM Tris-HCl pH 8.0 to deactivate free-floating biotin and incubated with streptavidin-AlexaFluor633 (Invitrogen) in PBS ...
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bioRxiv - Molecular Biology 2024Quote: ... Biotin conjugated EdU-labeled DNA was then pulled down using 5 μL of Dynabeads M-280 streptavidin (Invitrogen, 11205D). Beads were pre-washed three times with 1x Tween buffer (5 mM Tris HCl pH = 8 ...
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bioRxiv - Microbiology 2020Quote: ... cells were then washed 3 times with PBS and incubated with goat anti-rabbit Alexa Fluor 488 or goat anti-human Alexa Fluor 488 secondary antibodies (Thermo Fisher Scientific) for 1h at RT ...
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bioRxiv - Cancer Biology 2021Quote: ... Cells were washed three times with PBS on the next day and incubated with corresponding secondary antibodies goat anti-rabbit IgG (H+L) FITC (Thermo Fisher Scientific), goat anti-mouse IgM (H ...
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bioRxiv - Cancer Biology 2021Quote: ... coverslips were incubated for 1 hour at room temperature in blocking buffer containing the following secondary antibodies: Goat anti-Rabbit Alexa Fluor® 594 conjugate (1:250, #A-11037, Life Technologies) and Donkey anti-Mouse Alexa Fluor® 488 conjugate (1:250 ...
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bioRxiv - Cancer Biology 2021Quote: ... samples were incubated with secondary antibodies (FITC anti-mouse, 1:1000, Jackson Immuno Research, #715-545-150; AlexaFluor564 anti-rabbit, 1:500, Thermo Fisher, #A11035) for 2hr at RT ...
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bioRxiv - Neuroscience 2020Quote: ... the sections were blocked with goat/pig serum hybridized with an anti-GFP antibody (rabbit, Thermo Fisher Scientific, A-11122, 1:500), and then the signal was developed using the Rabbit Vectastain ABC HRP kit and diaminobenzidine substrate (Vector Inc. ...
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bioRxiv - Cell Biology 2020Quote: ... Cells were washed and incubated with Alexa Fluor 488 goat anti-rabbit (H+L) antibody (1:1,000; Thermo Fisher Scientific; A-11008) for 1 h at RT ...
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bioRxiv - Cell Biology 2020Quote: ... The sections were then incubated with an anti-rabbit/mouse secondary antibody conjugated with Alexa Fluor 594 (A-11037/A-21203, Invitrogen, Carlsbad, CA) and rinsed ...
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bioRxiv - Cell Biology 2020Quote: ... followed by incubation with donkey anti-rabbit secondary antibody coupled with red fluorescent Alexa Fluor 555 (1:400, Molecular Probes, ThermoFisher Scientific) for 2 hours at room temperature ...
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bioRxiv - Cell Biology 2020Quote: ... followed by incubation with donkey anti-rabbit secondary antibody coupled with red fluorescent Alexa Fluor 555 (1:400, Molecular Probes, ThermoFisher Scientific) for 2 hours at room temperature ...