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Citations for Thermo Fisher :
6351 - 6400 of 10000+ citations for Biotin Z Antibody Internalization Kit rabbit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2023Quote: ... and lamin was labeled with a custom-made rabbit polyclonal antibody (Wesolowska et al., 2020) primarily labeled with Alexa 488 NHS ester (Thermo Scientific) according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2023Quote: ... Secondary antibodies were: alexa-488 and alexa-546 -conjugated goat anti-mouse IgG or anti-rabbit IgG (Molecular Probes, Life Technologies). Nuclear counterstaining using DAPI (4’,6-diamino-2-phenylindole ...
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bioRxiv - Cell Biology 2023Quote: ... then with the following antibodies in 5% BSA for 1 h: donkey anti-rabbit IgG Alexa Fluor 488 (A21206, Thermo Fisher) and α-Bungarotoxin Alexa Fluor 594 (B13423 ...
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bioRxiv - Cell Biology 2023Quote: ... Secondary antibodies were: alexa-488 and alexa-546 -conjugated goat anti-mouse IgG or anti-rabbit IgG (Molecular Probes, Life Technologies). Nuclear counterstaining using DAPI (4’,6-diamino-2-phenylindole ...
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bioRxiv - Developmental Biology 2023Quote: ... Sections were then washed 2X for 10 minutes in PBS1X and incubated for 1h at room temperature with blocking solution containing the following fluorescent secondary antibodies: anti-mouse IgG Alexa Fluor 488 and anti-rabbit IgG Alexa Fluor 594 (Thermo Fisher) diluted 1:500 ...
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bioRxiv - Cell Biology 2023Quote: ... The following secondary antibodies were used accordingly: goat anti-rabbit Alexa Fluor 594 (IgG, 1:200, Cat#A-11037, Thermo Scientific) and goat anti-mouse Alexa Fluor 488 (IgG ...
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bioRxiv - Genetics 2023Quote: ... cells were incubated for 1 hour at room temperature with secondary antibodies (Alexa555 - donkey anti-rabbit and Alexa 488 - donkey anti-mouse, 1:2000, Thermofisher Scientific) and DAPI diluted in the same buffer ...
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bioRxiv - Genomics 2023Quote: ... cells were incubated with a secondary antibody (Anti-Rabbit Alexa Fluor-568: Invitrogen A10042; Anti-Mouse Alexa Fluor-488: Invitrogen #A11001) at 1:500 dilution in PBS-T for 1 hour at RT and then washed three times in PBS-T ...
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bioRxiv - Neuroscience 2024Quote: ... the slices were washed three times in 1X PBS and then incubated for 1 h at room temperature with a secondary antibody (Donkey anti-rabbit conjugated with Alexa 568, Thermo Fisher Scientific Cat# A10042 ...
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bioRxiv - Genetics 2023Quote: ... 10 mins) and resuspended in 3 mL MAGIC buffer supplemented with 60 µL Rabbit anti-Mouse IgM FITC Secondary Antibody (Invitrogen, 31557). The sample was gently rocked at 4 °C for 20 hrs ...
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bioRxiv - Cell Biology 2023Quote: ... cells were rinsed and incubated with the secondary antibodies conjugated with Alexa Fluor® 488 (anti-rabbit; 1:500, Molecular Probes) and Alexa Fluor® 594 (anti-mouse ...
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bioRxiv - Bioengineering 2023Quote: ... coli particles were resuspended in PBS and coated with rabbit polyclonal IgG antibodies (Escherichia coli BioParticles™ Opsonizing Reagent, Thermo Fisher) at 37°C for 1 hr ...
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bioRxiv - Neuroscience 2023Quote: ... mounted on Superfrost Plus glass slides and then incubated with the respective combinations of primary antibodies (Rabbit anti-GFP, 1:1000, A-6455, Thermo Fisher; Goat anti-tdTomato ...
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bioRxiv - Neuroscience 2023Quote: ... slices were washed with PBS three times for 10 minutes in PBS and incubated with secondary antibodies (respectively: 1:500 goat anti-rabbit IgG, Life Technologies, or 1:500 goat anti-mouse IgG1 ...
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bioRxiv - Neuroscience 2022Quote: ... 2 × 5 min) and incubated with secondary antibody (AlexaFluor 488-tagged goat anti-rabbit Ab, 1:1000, ThermoFisher Scientific, A-11008) and with Alexa Fluor 633-conjugated Streptavidin (1:400 ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... Cells were washed three times with PBS containing calcium and magnesium and then labeled with Alexa 488 goat anti-rabbit secondary antibody (1:1000; Invitrogen, #A11008) in a blocking buffer for 1 hr ...
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bioRxiv - Genomics 2022Quote: ... immunostained with an acetyl-Histone H3 (Lys9) rabbit antibody (Ac-H3K9, MA5-11195, 1:400, Thermo Fisher Scientific Inc., Waltham, MA) for 24 hours ...
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bioRxiv - Cell Biology 2023Quote: ... The secondary antibodies used in these experiments are goat anti-rabbit 488 and goat anti mouse H+L 568 (Life Technologies) at 1:1000 and goat anti guinea pig 568 (1:1000 ...
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bioRxiv - Microbiology 2023Quote: ... Samples were subsequently incubated with 10 % BSA to block the unspecific binding followed by incubation with a secondary α-rabbit antibody conjugated to Alexa Fluor 488 (Invitrogen) and diluted 1:1,000 (in PBS) ...
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bioRxiv - Immunology 2022Quote: ... formalin-fixed lung section was stained for rabbit polyclonal aSMA (Abcam #ab5694; diluted 1:750 in antibody diluent reagent solution [Life Technologies] ...
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bioRxiv - Microbiology 2023Quote: ... Sections were then incubated for 1 hour at room temperature in their corresponding goat anti-rabbit antibodies conjugated to Alexa Fluor 568 (1:1000, Thermofisher Scientific) and DAPI ...
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bioRxiv - Microbiology 2023Quote: ... The membranes were then incubated with goat anti-rabbit IgG (H+L) HRP conjugated secondary antibody (1:5000) (Thermofisher Scientific, 31460) for 1 hour at RT ...
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bioRxiv - Neuroscience 2023Quote: ... Secondary Antibodies (all used at 1:1000 dilution) were Alexa Fluor 568 goat anti-rabbit (Cat.# A11036, Thermofisher Scientific, Waltham, MA), Alexa Fluor 488 goat anti-chicken (Cat.# A11039 ...
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bioRxiv - Bioengineering 2023Quote: ... sections were counterstained for 1 hour at room temperature with secondary antibodies (donkey anti-rabbit Alexa Fluor 555 and donkey anti-mouse Alexa Fluor 647; 1:200; Thermo Fisher) and DAPI (1:1000 ...
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bioRxiv - Neuroscience 2022Quote: ... then processed for 48 hours at 4 degrees with secondary antibodies in blocking solution (1:1000, Alexa Fluor 647 goat anti-rabbit, Jackson Laboratories, plus 1:500 NeuroTrace, Invitrogen). Tissue sections were then rinsed again 3×10 minutes in PBS ...
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bioRxiv - Neuroscience 2023Quote: ... the sections were incubated with goat anti-rabbit IgG Alexa 488 secondary antibody (1:500 for 2 hours at 4°C; Invitrogen by ThermoFisher Scientific) in solution with PBS ...
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bioRxiv - Neuroscience 2023Quote: ... and incubated for 2 h with secondary antibodies diluted in blocking buffer to 1:600 (donkey anti-rabbit IgG Alexa Fluor 488, Invitrogen, Thermo Fisher, A21206 ...
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bioRxiv - Genomics 2023Quote: ... or 4 μl of rabbit polyclonal anti-GAF [101] antibodies per sample were immobilized on Protein G Dynabeads (Thermo Fisher Scientific), incubated with lysates for 90 min at 25°C ...
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bioRxiv - Cell Biology 2023Quote: ... the cells were incubated for 2 hours with secondary antibody AlexaFluor 488 Goat Anti-Rabbit IgG (1:1000 in 4% BSA, Invitrogen #A11008) at RT in the dark ...
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bioRxiv - Developmental Biology 2023Quote: ... for one hour at RT and stained overnight at 4°C with the primary antibody rabbit anti-GFP diluted 1:500 (#A11122 Thermo Fisher). Samples were washed three times using 0.3% PBT and stained with secondary antibody Alexa Fluor 488 Goat anti-Rabbit diluted 1:1000 (#R37116 ...
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bioRxiv - Immunology 2023Quote: ... whole-cleared tissues were washed for 3-4h-hours and incubated with a secondary Alexa Fluor™ 647 donkey anti-rabbit antibody (A-31573, Invitrogen) for another 3 days in room temperature ...
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bioRxiv - Cancer Biology 2023Quote: ... The sections were then washed three times in PBS-T followed by incubation with secondary antibodies (1:500) Alexa fluor (AF)-488 goat anti-rabbit IgG (ThermoFisher A32731) and AF-647 goat anti-chicken IgG (ThermoFisher A32933 ...
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bioRxiv - Cell Biology 2023Quote: ... cells were washed 3 times with PBS and AlexaFluor (AF488, AF568) conjugated anti-rabbit or anti-mouse antibodies (#A-11008, A-11004 Fisher Scientific) were added and incubated for 1h at room temperature (RT) ...
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bioRxiv - Cell Biology 2023Quote: ... The following secondary antibodies and dilutions were used: Alexa Fluor 568- and 647-conjugated goat anti-human and anti-rabbit (Life Technologies), 1:1000 ...
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bioRxiv - Genetics 2023Quote: ... Labeling with the secondary antibodies was performed in 150 μl 1×PBS/EDTA for two hours on ice using Alexa fluor 488 (A488) or Alexa fluor 647 (A647) conjugated goat anti-mouse IgG or goat anti-rabbit IgG antibodies (Thermo Fisher Scientific ...
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bioRxiv - Evolutionary Biology 2023Quote: ... Slides were washed three times in 1× PBS at RT and incubated in a combination of secondary antibodies (Cy3-conjugated goat anti-rabbit IgG (H+L) (Molecular Probes) and Alexa-488-conjugated goat anti-mouse IgG (H+L ...
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bioRxiv - Developmental Biology 2023Quote: ... samples were incubated for 2 h at room temperature with secondary antibody Alexa 488 goat anti-rabbit IgG or rhodamine-phalloidin (1:500, Invitrogen, Molecular Probes). Samples were washed three times in 0.5% PBS-T and mounted in Vectashield containing DAPI.
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bioRxiv - Cancer Biology 2023Quote: ... Following further washes both secondary antibodies Alexa Fluor 488 (anti-rabbit) and Alexa Fluor 546 (anti-mouse) (Invitrogen, Waltham, MA, USA) diluted 1:1000 were added to coverslips for 1 hr at room temperature in dark conditions before coverslips were washed and exposed to 0.5 μg/ml DAPI solution prior to mounting coverslips onto microscope slides using Prolong Glass Antifade mountant (Thermofisher ...
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bioRxiv - Cell Biology 2023Quote: ... to detect HA-tagged TRPM2 channel domain and the lower part of the membrane was incubated with rabbit-anti-FLAG (DYKDDDDK) antibody (1:1000; 701629; Invitrogen, USA) to detect FLAG-tagged NUDT9H domain and NUDT5 ...
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bioRxiv - Cell Biology 2023Quote: ... slides were washed with PBS and incubated for 2 h at room temperature with the secondary antibody (goat anti-rabbit IgG H+L conjugated with Alexa Fluor 488, Thermo Scientific), washed with PBS and mounted in VECTASHIELD® HardSet™ antifade medium with DAPI (Vector Laboratories ...
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Autoimmune inflammation triggers aberrant astrocytic calcium signaling to impair synaptic plasticitybioRxiv - Neuroscience 2023Quote: ... The sections were then washed in PBS for 30 min at RT and primary antibodies were detected by incubation with donkey anti-rabbit Alexa 647 (1:500; Invitrogen, A31573) and donkey anti-chicken Rhodamine Red (1:500 ...
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bioRxiv - Neuroscience 2023Quote: ... Secondary goat anti-mouse and anti-rabbit were used according to primary antibodies (1:1,000; A11001, A32727, A21235, A32731, A21428, and A32733; Molecular Probes, Invitrogen). Secondary goat anti-rat (1:1,000 ...
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bioRxiv - Neuroscience 2023Quote: ... Sections were washed (0.2% Triton X-100 in PBS) and incubated with corresponding goat anti-rabbit/mouse secondary antibodies (1:1,000; A32731, A32733, and A21235; Molecular Probes, Invitrogen) for 3 h at room temperature ...
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bioRxiv - Neuroscience 2023Quote: ... the virally expressed GFP or YFP was enhanced using the rabbit anti-GFP Tag Polyclonal Antibody-Alexa Fluor 488 (Invitrogen #A21311). In the viral rescue experiment (Figure 5A-B) ...
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bioRxiv - Neuroscience 2023Quote: ... Sections were washed with 0.1 M PBS containing 0.3% triton X-100 then incubated in secondary antibodies (donkey anti-rabbit Alexa-fluor 594, Invitrogen A21207 and donkey anti chicken Alexa-fluor 647, Invitrogen A78952) diluted 1:200 in block buffer at room temperature for 1 hour ...
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bioRxiv - Molecular Biology 2023Quote: ... cells were washed and incubated for 45 min at 37 °C with the goat anti-rabbit secondary antibody (1:250, Thermo Scientific), also diluted in 3% BSA in PBS.
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bioRxiv - Systems Biology 2023Quote: ... Sections were then washed with the wash buffer thrice for 10 min each time and incubated with an poly-HRP-conjugated secondary antibody solution (SuperBoost™ Goat anti-Rabbit Poly HRP, IgG, Invitrogen) for 1 h at room temperature with gentle shaking ...
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bioRxiv - Neuroscience 2023Quote: ... slices were incubated for 2 h at room temperature in secondary antibody goat anti-rabbit IgG (H+L) Alexa 647 (Invitrogen, A32733) diluted 1:500 in PB 0.1 M containing 0.3% Triton X-100 and 3% NGS.
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bioRxiv - Neuroscience 2023Quote: ... we washed 5 times with PB 0.1 M and incubated them for 1 h at room temperature in secondary antibody goat anti-rabbit IgG (H+L) Alexa 647 (Invitrogen, A32733) diluted 1:1000 in PB 0.1 M containing 1% NGS ...
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bioRxiv - Neuroscience 2023Quote: ... sections were incubated with the following fluorophore-conjugated secondary antibodies for 2 h at room temperature: anti-rabbit Alexa Fluor 488 (1:400, goat, Invitrogen, A11008), anti-rabbit Alexa Fluor 568 (1:400 ...