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Citations for Thermo Fisher :
6601 - 6650 of 10000+ citations for Biotin Z Antibody Internalization Kit rabbit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2020Quote: ... membranes were incubated with the respective secondary donkey anti-rabbit IgG highly cross-adsorbed (A10043) or anti-mouse 680 Alexa Fluor antibodies (A21058, Thermo Fisher Scientific). Membranes were washed three times with PBS containing 0.05% Tween-20 and visualized on the Odyssey infrared imaging system ...
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bioRxiv - Cell Biology 2020Quote: ... 1000 for overnight at 4°C and then incubated with respective HRP-labelled secondary antibodies [(anti-Rabbit IgG; 656120; Thermo Fisher Scientific), (anti-Mouse IgG ...
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bioRxiv - Cell Biology 2020Quote: ... was used at a 1:1000 dilution overnight at 4°C followed by incubation with fluorescently tagged goat anti mouse-A647 and donkey anti-rabbit-A555 secondary antibodies at 1:100 dilution (Invitrogen, Carlsbad, CA). TUNEL staining to detect apoptotic cells was completed prior to antibody stainig per manufactuors instructions (Promega ...
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bioRxiv - Cell Biology 2020Quote: ... the plate was washed with 200 μL PBST for 4 times and 100 μL HRP-linked goat anti-rabbit IgG secondary antibody (Invitrogen™ #31460) at 0.4 μg/ml concentration in PBST buffer was added for 1 h incubation in the dark ...
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bioRxiv - Developmental Biology 2021Quote: ... domes were washed in PBS containing 0.1% Triton X-100 and incubated in Alexa Fluor 568 conjugated secondary donkey anti-rabbit antibody (Invitrogen: A10042; 1:1000) for 4h at room temperature ...
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bioRxiv - Genetics 2020Quote: ... abdomens of control flies and flies with CRISPR/Cas9 generated GFP-tagged GR8A were dissected and immunostained as previously described (Lu et al., 2012; Zheng et al., 2014) using a Rabbit anti-GFP antibody (1:1000; A-11122, Thermo Fisher Scientific) followed by AlexaFluor 488 anti-rabbit secondary antibody (1:1000 ...
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bioRxiv - Neuroscience 2021Quote: ... Cryosections of the cochlea and brain slices were washed in PBS for 1 h and subsequently incubated in secondary antibody solution containing blocking solution and the secondary antibodies Alexa Fluor 488-conjugated goat anti-rabbit (1:1000, cochlear cryosections) (Molecular Probes (A11008)) ...
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bioRxiv - Developmental Biology 2021Quote: ... cells were washed and incubated with the Alexa fluor 488-conjugated donkey anti-mouse and/or 594-conjugated donkey anti-rabbit secondary antibodies (1: 400; Thermo Fisher Scientific) for 1 hour ...
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bioRxiv - Developmental Biology 2021Quote: ... We used anti-rabbit or anti-mouse Alexa Fluor 594 or Alexa Fluor 488 conjugated secondary antibodies (1:1000, Invitrogen, California, USA) and captured the images on a Nikon Eclipse 90i ...
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bioRxiv - Evolutionary Biology 2021Quote: ... Sections were then washed three times in PBS containing 0.5% Tween 20 (v/v) then incubated for 45 min with goat anti-rabbit Alexa Fluor Plus 555 secondary antibody (A32732, Invitrogen, ThermoFisher Scientific) at either 1:300 dilution (SHH) ...
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bioRxiv - Developmental Biology 2020Quote: ... 1:1000 dilution), Alexa Fluor 647-conjugated goat anti-rabbit IgG (H+L) antibody (#A32733, 1:1000 dilution)(all Thermo Fisher Scientific).
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bioRxiv - Systems Biology 2022Quote: ... Cells were immunostained according to standard protocols [21] using a primary rabbit anti-human antibody directed against zona occludens-1 (ZO-1; 1:100, Thermo-Fisher Scientific, UK) and a AF488-conjugated secondary goat anti-rabbit antibody (1:500 ...
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bioRxiv - Neuroscience 2022Quote: ... and in the secondary antibody diluted in 10% normal donkey serum for 2 hours at room temperature [Secondary antibody: Alexa Fluor 647 goat anti-rabbit (1:500; Thermo Fisher Scientific)].
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bioRxiv - Neuroscience 2022Quote: ... and in secondary antibodies diluted in 10% normal donkey serum for 1 hour at room temperature [Secondary antibodies: Alexa Fluor 405 goat anti-rabbit (1:500; Thermo Fisher Scientific) and Alexa Fluor 647 goat anti-mouse IgG1 (1:500 ...
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bioRxiv - Biophysics 2022Quote: ... The blot was then incubated for 10 min at room temperature with a 1:1000 dilution of rabbit polyclonal anti-ASIC1a antibody (OSR00097W, Thermo Fisher Scientific) in blocking buffer followed by 10 min incubation at room temperature with goat anti-rabbit IgG/horseradish peroxidase-conjugate (1:10,000 dilution of 1 mg/mL stock in blocking buffer ...
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bioRxiv - Biochemistry 2022Quote: ... and probed for 2 h with an FITC-conjugated goat anti-rabbit antibody (1:300) and the endothelial cell-specific marker Alexa 647-Isolectin B4 (1:1000; Thermo Fisher Scientific). The number of NG2-positive pericytes was counted in the superficial vascular plexus of the retina per mm2 vascular area.
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bioRxiv - Neuroscience 2021Quote: ... Secondary antibodies were incubated in 1% NGS in wash buffers at 4°C with agitation with a 1:400 dilution at the same durations as the first antibodies (goat anti-mouse-Alexafluor-488, A-11017; goat anti-rabbit-Alexafluor-555, A-21430; ThermoFisher Scientific/Invitrogen, MA, USA). Then ...
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bioRxiv - Neuroscience 2021Quote: ... for 15 min at room temperature with the secondary antibodies [Alexa488-conjugated anti-rabbit IgG and Alexa647-conjugated anti-mouse IgG: 1:500 (Thermo Fisher, USA)] ...
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bioRxiv - Neuroscience 2021Quote: ... followed by 2 hr incubation at RT in secondary antibody diluted 1:500 in block (goat anti-rabbit Alexa 568, Life Technologies; 1:500 goat anti-chicken Alexa 488, Life Technologies). Sections were again washed in TBS ...
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bioRxiv - Neuroscience 2021Quote: ... followed by overnight incubation at 4 °C in secondary antibody diluted 1:500 in block (donkey anti-rabbit Alexa 568, Life Technologies A10042). Sections were again washed in TBS ...
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bioRxiv - Molecular Biology 2021Quote: ... membranes were washed in 1X PBST and incubated with goat anti-rabbit Alexa Fluor 680 and/or goat anti-mouse DyLight 800 secondary antibodies (Thermo Fisher Scientific) for 1 hour at room temperature ...
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bioRxiv - Molecular Biology 2021Quote: ... Input samples were taken before incubating the rest of the cell lysates overnight with 10 µg rabbit anti-GFP antibody (Thermo Fisher Scientific) and Protein G Dynabeads ...
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bioRxiv - Molecular Biology 2021Quote: ... 2008) at 4°C overnight and then incubated with Alexa Fluor 488-conjugated anti-rabbit antibody (1/800, Life Technologies, Oregon, USA). For in situ hybridization of the mouse Mesp1 and Mesp2 mRNA ...
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bioRxiv - Molecular Biology 2021Quote: ... squashes were washed in PBST and incubated in secondary antibody (Alexa Fluor 488-conjugated goat anti-rabbit and Alexa Fluor 568–conjugated goat anti--mouse IgG antibodies, 1:500; Thermo Fisher Scientific) in blocking solution for 1h ...
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bioRxiv - Neuroscience 2020Quote: ... Alexa Fluor conjugated secondary antibodies (Donkey anti-Rabbit 488, Thermo Fisher Scientific, Cat#: R37118; Goat anti-Guinea Pig Alexa Fluor 488, Thermo Fisher Scientific ...
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bioRxiv - Genomics 2020Quote: ... LECs were washed three times for 5min in DPBS and subsequently stained with donkey Alexa488-conjugated anti-rabbit secondary antibody (Thermo Fisher Scientific) diluted 1:1000 ...
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bioRxiv - Genomics 2020Quote: ... at a dilution of 1:100 overnight at 4°C followed by donkey Alexa488-conjugated anti-rabbit secondary antibody (Thermo Fisher Scientific) at a dilution of 1:200 for 30min at room temperature ...
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bioRxiv - Genomics 2020Quote: ... SEMA3C protein levels were checked by western blot as described above using rabbit anti-human SEMA3C antibody (1:1000, Thermo Fisher Scientific). Beta-actin (housekeeping gene ...
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bioRxiv - Cell Biology 2019Quote: ... Secondary antibodies (Alexa Fluor 488-, 594-, or 647-goat α–rabbit IgG, Alexa Fluor 488-, 594-, or 647-goat α–mouse IgG (Invitrogen)) were used at 1:1000.
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bioRxiv - Neuroscience 2019Quote: ... For visualization horseradish peroxidase-conjugated secondary antibodies were used (goat anti-mouse: Jackson ImmunoResearch Laboratries, 115-035-062. Goat-anti rabbit: Invitrogen, A21245).
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bioRxiv - Neuroscience 2019Quote: ... 40 µm sections were collected using a cryostat and stained with primary antibody overnight at 4° C (1:1000 rabbit α-GFP, Life Technologies) and incubated with secondary antibodies (2h at room temperature ...
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bioRxiv - Plant Biology 2019Quote: ... SDS-PAGE was followed by western blotting with a 1:10,000 dilution of rabbit anti-GFP antibody (Invitrogen Molecular Probes, Eugene, OR). A secondary goat anti-rabbit horseradish peroxidase antibody (Bio-Rad Laboratories ...
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bioRxiv - Plant Biology 2019Quote: ... SDS-PAGE was followed by western blotting with a 1:10,000 dilution of rabbit anti-GFP antibody (Invitrogen Molecular Probes, Eugene, OR). A secondary goat anti-rabbit horseradish peroxidase antibody (Bio-Rad Laboratories ...
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bioRxiv - Developmental Biology 2020Quote: ... Excess of antibody was removed by three washes in TBS-T followed by incubation with the secondary antibody (Goat anti-Rabbit IgG Alexa Flour 488; # A-11034; Thermo Fisher Scientific) at a 1:500 dilution in blocking buffer in dark for 2.5h at RT ...
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bioRxiv - Molecular Biology 2019Quote: ... Cells were washed three times with PBS supplemented with 0.2% tween-20 and were incubated fluorophore-conjugated secondary antibodies (1:1000 dilutions of mouse-Alexa 488 and rabbit-Alexa647 (Thermo Fisher Scientific)) in Blocking buffer for one hour at room temperature ...
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bioRxiv - Biophysics 2019Quote: ... Cultures were then incubated overnight at 4°C with primary antibodies at a concentration of 1:500 for rabbit anti-MAP7 (Thermofisher PA5-31782), 1:500 for mouse monoclonal anti-Tau-1 (Millipore MAB3420) ...
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bioRxiv - Molecular Biology 2019Quote: ... 2016) were used as a 1/500 dilution and detected with goat anti-rabbit antibody (Alexa-Fluor 488; Molecular Probes; 1/1,000). Nuclei were stained with ToPRO 3-Iodide (Molecular Probes) ...
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Cell culture dimensionality influences mesenchymal stem cell fate through cadherin-2 and cadherin-11bioRxiv - Cell Biology 2019Quote: ... Secondary antibodies used were goat anti-mouse Alexa Fluor 488 and goat anti-rabbit Alexa Fluor 647 (both 1:500; Thermo Fisher Scientific).
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Cell culture dimensionality influences mesenchymal stem cell fate through cadherin-2 and cadherin-11bioRxiv - Cell Biology 2019Quote: ... Primary antibodies used were rabbit polyclonal anti-cadherin-11 and mouse monoclonal anti-cadherin-2 (3B9) (both 1:100; Thermo Fisher Scientific). Secondary antibodies used were goat anti-mouse Alexa Fluor 488 and goat anti-rabbit Alexa Fluor 647 (both 1:500 ...
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bioRxiv - Genomics 2020Quote: ... and Alexa-Fluor® conjugated secondary antibodies (1:1000 dilution for 4 hours, anti-rabbit Alexa-Fluor®-647, ThermoFisher Z25308; anti-mouse Alexa-Fluor®-647, ThermoFisher Z25008 ...
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bioRxiv - Genomics 2020Quote: ... Key Resources Table) and Alexa-Fluor® conjugated secondary antibodies (1:1000 dilution for 4 hours, anti-rabbit Alexa-Fluor®-647, ThermoFisher Z25308 ...
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bioRxiv - Cancer Biology 2019Quote: ... and incubated with appropriate secondary antibody (1:100 anti-mouse IgG3 Alexa 488 and 1:300 goat anti rabbit Alexa 568; Thermo Fisher Scientific). Nuclei were counterstained with DAPI solution ...
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bioRxiv - Neuroscience 2019Quote: ... cells were rinsed and incubated with the corresponding secondary antibodies: Alexa Fluor® 488 (anti-mouse or anti-rabbit; 1:500, Molecular Probes), Alexa Fluor® 594 (anti-mouse or anti-rabbit ...
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bioRxiv - Microbiology 2019Quote: ... The biofilm was then washed with 3% BSA-PBS and incubated with secondary antibody (IgG horseradish peroxidase-conjugated anti-rabbit or anti guinea-pig, Thermo Scientific Singapore) at 1:500 dilution for 30 minutes and washed with 3% BSA-PBS ...
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bioRxiv - Pathology 2019Quote: ... blots were incubated 1 h with horseradish peroxidase (HRP)-conjugated goat anti-rabbit IgG secondary antibody (1:20000; Invitrogen cat. Number 31460) in blocking buffer ...
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bioRxiv - Microbiology 2019Quote: ... fixed with 4% PFA for 20 min at room temperature and incubated with secondary antibody (Alexa Fluor 488-conjugated goat anti-rabbit, Thermo Fisher, A21441). The images are single plane images taken with a Leica SP5 Confocal Microscope using a water-immersion 63× objective.
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bioRxiv - Neuroscience 2019Quote: ... sections were incubated 2 hours at room temperature with the corresponding secondary antibodies—Alexa fluor 633 conjugated goat anti-rabbit (1:500; ThermoFisher A-21070) and Alexa fluor 633 conjugated goat anti-mouse (1:500 ...
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bioRxiv - Pharmacology and Toxicology 2019Quote: ... Proteins were detected with horseradish peroxidase-conjugated secondary antibody (1:5,000; goat anti-rabbit (Thermo Fisher Scientific #31460 or 1:3,000; goat anti-mouse; Thermo Fisher Scientific #31430) and Clarity Western ECL Blotting Substrate (Bio-Rad).
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bioRxiv - Molecular Biology 2019Quote: ... and pol μ antibodies were affinity purified from rabbit serum by coupling each full-length recombinant protein to AminoLink Coupling Resin (Thermo Scientific) and following manufacturer’s instructions for IgG purification.
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bioRxiv - Neuroscience 2020Quote: ... 1:1000 anti-Sox2 polyclonal antibody overnight followed by 1:500 Alexa Fluor 488 and 1:500 Alexa Fluor 694 goat anti-rabbit IgG (Invitrogen, A-11034).