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Citations for Thermo Fisher :
6401 - 6450 of 10000+ citations for Biotin Z Antibody Internalization Kit rabbit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2023Quote: ... slides were washed with PBS and incubated for 2 h at room temperature with the secondary antibody (goat anti-rabbit IgG H+L conjugated with Alexa Fluor 488, Thermo Scientific), washed with PBS and mounted in VECTASHIELD® HardSet™ antifade medium with DAPI (Vector Laboratories ...
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bioRxiv - Cancer Biology 2023Quote: ... Following further washes both secondary antibodies Alexa Fluor 488 (anti-rabbit) and Alexa Fluor 546 (anti-mouse) (Invitrogen, Waltham, MA, USA) diluted 1:1000 were added to coverslips for 1 hr at room temperature in dark conditions before coverslips were washed and exposed to 0.5 μg/ml DAPI solution prior to mounting coverslips onto microscope slides using Prolong Glass Antifade mountant (Thermofisher ...
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bioRxiv - Genetics 2023Quote: ... Labeling with the secondary antibodies was performed in 150 μl 1×PBS/EDTA for two hours on ice using Alexa fluor 488 (A488) or Alexa fluor 647 (A647) conjugated goat anti-mouse IgG or goat anti-rabbit IgG antibodies (Thermo Fisher Scientific ...
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bioRxiv - Neuroscience 2023Quote: ... Secondary goat anti-mouse and anti-rabbit were used according to primary antibodies (1:1,000; A11001, A32727, A21235, A32731, A21428, and A32733; Molecular Probes, Invitrogen). Secondary goat anti-rat (1:1,000 ...
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bioRxiv - Neuroscience 2023Quote: ... Sections were washed (0.2% Triton X-100 in PBS) and incubated with corresponding goat anti-rabbit/mouse secondary antibodies (1:1,000; A32731, A32733, and A21235; Molecular Probes, Invitrogen) for 3 h at room temperature ...
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Autoimmune inflammation triggers aberrant astrocytic calcium signaling to impair synaptic plasticitybioRxiv - Neuroscience 2023Quote: ... The sections were then washed in PBS for 30 min at RT and primary antibodies were detected by incubation with donkey anti-rabbit Alexa 647 (1:500; Invitrogen, A31573) and donkey anti-chicken Rhodamine Red (1:500 ...
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bioRxiv - Evolutionary Biology 2023Quote: ... Slides were washed three times in 1× PBS at RT and incubated in a combination of secondary antibodies (Cy3-conjugated goat anti-rabbit IgG (H+L) (Molecular Probes) and Alexa-488-conjugated goat anti-mouse IgG (H+L ...
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bioRxiv - Immunology 2023Quote: ... whole-cleared tissues were washed for 3-4h-hours and incubated with a secondary Alexa Fluor™ 647 donkey anti-rabbit antibody (A-31573, Invitrogen) for another 3 days in room temperature ...
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bioRxiv - Neuroscience 2023Quote: ... the virally expressed GFP or YFP was enhanced using the rabbit anti-GFP Tag Polyclonal Antibody-Alexa Fluor 488 (Invitrogen #A21311). In the viral rescue experiment (Figure 5A-B) ...
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bioRxiv - Immunology 2023Quote: ... 1:100 fold diluted Goat anti-Rabbit IgG (H+L) Cross-Adsorbed Secondary Antibody (Alexa Fluor™ 488, ThermoFisher, Cat.#R37116) was then added and incubated at room temperature for 1 h ...
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bioRxiv - Immunology 2023Quote: ... Expression of IBA1 was then visualized using a secondary antibody against rabbit IgG conjugated to Alexa-Fluor 488 (Life Technologies A21206) at a 1:500 dilution in Co-Detection Antibody Diluent for 30 minutes at RT in the dark followed by 3 washes with PBS-T ...
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bioRxiv - Biochemistry 2023Quote: ... After three washes with TBS-T the cells were incubated for 1 hr at room temperature with the secondary antibody Alexa Fluor 488-labeled anti-rabbit at 1:1,000 dilution (A-11008, Thermo Fisher Scientific). After three washes in TBS-T ...
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bioRxiv - Biochemistry 2023Quote: ... Cells were then incubated at RT for 2 hours with 1:1000 Alexa Fluor (AF) 647 donkey anti-mouse and 1:200 of AF488 donkey anti-rabbit fluorescence conjugated secondary antibodies (Life Technologies). The cover slip was mounted in mounting solution with DAPI ...
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bioRxiv - Bioengineering 2022Quote: ... The spheroids were then washed thoroughly in PBS and incubated in Alexa Fluor 594®-conjugated goat anti-rabbit IgG secondary antibody (A11037, Thermofisher) at a 1:500 dilution in blocking solution at 4 °C overnight ...
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bioRxiv - Cell Biology 2022Quote: ... and incubated with the specific secondary antibody: goat anti-mouse Alexa Fluor-488 or goat anti-rabbit Alexa Fluor-594 (Molecular Probes). The incubation with secondary antibodies were accomplished in a humidified chamber for 1 h at RT ...
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bioRxiv - Bioengineering 2023Quote: ... then incubated with the corresponding secondary antibody (Alexa-488 conjugated goat anti-mouse and Alexa-594 conjugated goat anti-rabbit (Life Technologies)) (1:150 ...
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bioRxiv - Cell Biology 2022Quote: ... for 2 h at RT followed by PBS washes and incubation with 1:300 diluted secondary antibody (goat anti-rabbit coupled to Alexa488, Thermo Scientific). After a final PBS wash ...
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bioRxiv - Cell Biology 2023Quote: ... the membranes were washed 3 times in TBS-T for 10 minutes each and incubated with an alkaline phosphatase-linked secondary goat anti-mouse/anti-rabbit antibody (1:10,000; Thermo Scientific Pierce) at RT for 1h ...
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bioRxiv - Cell Biology 2023Quote: ... the coverslips were washed 4 times with PHEM containing 0.05% Tween-20 and incubated with appropriate Alexa Fluor 633-conjugated secondary antibodies (Goat anti-Mouse, Invitrogen A21052 or Goat anti-Rabbit, Invitrogen A21071) at a 1:10000 dilution for 45 min at room temperature in the dark ...
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bioRxiv - Cancer Biology 2023Quote: ... The horseradish peroxidase (HRP)-conjugated Protein A (#101023) and HRP-conjugated rabbit anti-mouse secondary antibody (#31430) for immunoblotting were from Thermo Fisher. Antibodies used for immunofluorescence studies were as follows ...
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bioRxiv - Cell Biology 2022Quote: ... antibodies were used followed by specific secondary Alexa Fluor antibodies (1:500 anti-mouse Alexa Fluor 546 and 1:500 anti-rabbit Alexa 488 Thermo Fisher). For actin staining ...
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bioRxiv - Developmental Biology 2023Quote: ... The secondary antibodies Alexa 488-conjugated goat anti-mouse and Alexa-568-conjugated goat anti-rabbit (Molecular Probes, Waltham, MA, USA) were diluted 1:400 and 1:1000 ...
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bioRxiv - Developmental Biology 2023Quote: ... 1:1000) Alexa Fluor 546-conjugated and anti-rabbit (A-31573, 1:1000) Alexa Fluor 647-conjugated antibodies (both from Thermo Fisher).
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bioRxiv - Developmental Biology 2023Quote: ... slides were incubated 45-60 min at room temperature with secondary antibodies in PBT (1:500 goat anti-rabbit-Alexa488 and goat anti-mouse-Alexa568 (ThermoFisher Scientific). Slides were counterstained with 1 μg/ml Hoechst 33258 (Sigma ...
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bioRxiv - Immunology 2023Quote: ... in the same blocking solution overnight for 16 h and then with Alexa Fluor 594-conjugated donkey anti-rabbit IgG secondary fluorescent antibody (1:500 dilution, Invitrogen A21207) for 2 h at room temperature ...
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bioRxiv - Neuroscience 2023Quote: ... Rpl22HA-containing ribosomes were immunoprecipitated (“IP”) from the remaining supernatant by gently mixing with 5µl of rabbit anti-HA antibody (#71–5500 Thermo Fisher Sci) and incubating with gentle rocking at 4°C for 2 hours before isolation with 50 µl of protein A/G magnetic beads previously equilibrated in polysome buffer and a magnetic stand ...
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bioRxiv - Cancer Biology 2022Quote: ... Proliferation index within metastatic foci was calculated by the staining with a rabbit anti-mouse Ki67 antibody (clone SP6, Thermo Fisher). For CD45 staining ...
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bioRxiv - Neuroscience 2023Quote: ... The following primary antibody (diluted in the blocking solution) was used: rabbit anti–PGP9.5 (1:400, Thermo Fisher Scientific, #PA5-29012), rabbit anti-S100β (1:500 ...
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bioRxiv - Developmental Biology 2023Quote: ... embryos were incubated in blocking buffer containing secondary antibodies (Alexa Fluor 555 donkey anti-rabbit IgG, 1:500 dilution, A31572, Thermo Fisher Scientific ...
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bioRxiv - Cancer Biology 2023Quote: ... The cells were then washed with cold 1X PBS three times for 3 min each and incubated with Alexa 555-labeled anti-rabbit and Alexa 488-labeled anti-mouse secondary antibody (Thermo Fisher) at room temperature for 1 hour ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... and 50 μL of a 1:4,000 dilution of rabbit anti-SARS-CoV-2 nucleocapsid antibody (Invitrogen, Waltham, MA, Cat# MA536086) in blocking buffer was added and incubated for 2 h at 37°C ...
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bioRxiv - Neuroscience 2023Quote: ... then stained with Alexa Fluor 488 or 555 goat anti-rabbit IgG secondary antibody (1:500; Thermo Fisher Scientific, MA, USA) in 1x PBST for 1 h at room temperature ...
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bioRxiv - Biochemistry 2023Quote: ... in PBS containing 5% normal goat serum for 30 min at RT followed by incubation with goat anti-chicken and anti-rabbit secondary antibodies (1:400, A-11039, A-11011, Invitrogen, USA) for 30 min at RT ...
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bioRxiv - Microbiology 2023Quote: ... diluted at 1:250 in blocking solution was incubated 1h at room temperature followed by a 45min incubation of a goat anti-rabbit Alexa-488 secondary antibody (Invitrogen, #A11034) diluted to 1:500 together with Hoechst and rhodamine-phalloidin ...
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bioRxiv - Neuroscience 2023Quote: ... Slides were incubated with secondary antibodies of goat-anti-rat 488 and goat-anti-rabbit 594 (1:500, Invitrogen, A11006, A11037) 1.5 hours in room temperature ...
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bioRxiv - Cancer Biology 2023Quote: ... Cells were then washed three times and incubated with Alexa 488/647-labeled anti-mouse/rabbit secondary antibody (Invitrogen, A21202, A31573) for 1h at RT ...
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bioRxiv - Immunology 2023Quote: ... The following secondary antibodies were used at 1:300 dilution: Alexa Fluor 555 donkey anti-rabbit IgG (H+L) cross-adsorbed (ThermoFisher Scientific), Alexa Fluor 647 donkey anti-rat IgG (H+L ...
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bioRxiv - Molecular Biology 2023Quote: ... with AF488-conjiugated secondary antibodies diluted 1:3000 in BPS (goat anti-mouse IgG, A11001 or goat anti-rabbit IgG, A11008, Life Technologies). For dual-color IF ...
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bioRxiv - Neuroscience 2023Quote: ... sections were washed and incubated with secondary antibody diluted in blocking solution: Goat anti-Rabbit IgG Alexa-488 (Invitrogen A-11008), Goat anti-Rat IgG Alexa 594 (Invitrogen A-11007) ...
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bioRxiv - Genomics 2023Quote: ... or 4 μl of rabbit polyclonal anti-GAF [101] antibodies per sample were immobilized on Protein G Dynabeads (Thermo Fisher Scientific), incubated with lysates for 90 min at 25°C ...
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bioRxiv - Biochemistry 2023Quote: ... followed by probing with secondary antibodies (goat anti-rabbit Alexafluor 488, A11034, 1:500; goat anti-rat Alexafluor 647, A21247, 1:500, ThermoFisher Scientific) applied and washed as per the primary antibodies ...
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bioRxiv - Cell Biology 2023Quote: ... cells were rinsed several times with PBS and incubated with secondary antibodies (donkey anti-rabbit Alexa Fluor 647 Plus [PIA32795; Thermo Fisher Scientific] ...
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bioRxiv - Neuroscience 2023Quote: ... the sections were rinsed four times for 20 minutes each with new PBST solution before incubation in a secondary antibody solution containing a 1:1,000 dilution of Goat anti Rabbit-AlexaFluor568 (Invitrogen, A-11011) or Goat anti-chicken AlexaFlour 488(Invitrogen ...
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bioRxiv - Developmental Biology 2023Quote: ... Sections were then incubated with secondary antibodies at room temperature for 2 h at 1:500 dilution: anti-rabbit AF594 (Invitrogen, A21207); anti-rat AF488 (Invitrogen ...
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bioRxiv - Developmental Biology 2023Quote: ... embryos were incubated overnight at 4°C with secondary antibodies conjugated with Alexa Fluor 633 (1:500; Goat anti-Rabbit Invitrogen A21070, Goat anti-Mouse Invitrogen A21053).
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bioRxiv - Developmental Biology 2023Quote: ... for one hour at RT and stained overnight at 4°C with the primary antibody rabbit anti-GFP diluted 1:500 (#A11122 Thermo Fisher). Samples were washed three times using 0.3% PBT and stained with secondary antibody Alexa Fluor 488 Goat anti-Rabbit diluted 1:1000 (#R37116 ...
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bioRxiv - Cell Biology 2023Quote: ... The following secondary antibodies and dilutions were used: Alexa Fluor 568- and 647-conjugated goat anti-human and anti-rabbit (Life Technologies), 1:1000 ...
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bioRxiv - Cell Biology 2023Quote: ... All samples were incubated with the secondary antibody conjugated with donkey anti-rabbit Alexa Fluor Plus 488 (Invitrogen, A32790, 1:500) and donkey anti-mouse Alexa Fluor Plus 594 (Invitrogen ...
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bioRxiv - Developmental Biology 2023Quote: ... sections were incubated for 1 hour at room temperature with the primary rabbit polyclonal antibody anti-CD3 (1:100; ThermoFisher Scientific), then incubated for 30 minutes with MACH 1™ Universal HRP Polymer Kit (Biocare Medical) ...
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bioRxiv - Genomics 2023Quote: ... washed with 1XPBS then incubated for 40 min at RT with Alexa Fluor 488 nm anti-rabbit or Alexa Fluor 594 nm anti-mouse secondary antibodies (Life Technologies). Cells were then washed in 1XPBS and mounted in Vectashield plus DAPI (Vector Laboratories) ...